期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 27, 页码 11085-11089出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0904716106
关键词
5-fold averaging; cryoelectron microscopy reconstruction; Paramecium bursaria Chlorella virus-1; cell entry; specialized vertex protein
资金
- National Institutes of Health [AI11219, GM32441, GM33050]
Paramecium bursaria Chlorella virus-1 is an icosahedrally shaped, 1,900-angstrom-diameter virus that infects unicellular eukaryotic green algae. A 5-fold symmetric, 3D reconstruction using cryoelectron microscopy images has now shown that the quasiicosahedral virus has a unique vertex, with a pocket on the inside and a spike structure on the outside of the capsid. The pocket might contain enzymes for use in the initial stages of infection. The unique vertex consists of virally coded proteins, some of which have been identified. Comparison of shape, size, and location of the spike with similar features in bacteriophages T4 and P22 suggests that the spike might be a cell-puncturing device. Similar asymmetric features may have been missed in previous analyses of many other viruses that had been assumed to be perfectly icosahedral.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据