Article
Microbiology
Jing Xu, Jie Gao, Maolin Zhang, Danwei Zhang, Ming Duan, Zhenhong Guan, Yidi Guo
Summary: Rabies virus infects N2a cells in a manner dependent on microtubule integrity as well as the function of dynein and kinesin. Microtubule-depolymerizing drugs, dynein inhibitors, and disruption of kinesin function can all hinder Rabies virus infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Agata Gluszek-Kustusz, Benjamin Craske, Thibault Legal, Toni McHugh, Julie P. Welburn
Summary: During mitosis, the microtubule crosslinker protein PRC1 recruits motor proteins CENP-E and Kif4A to the central spindle to coordinate chromosome segregation and cytokinesis. This recruitment is mediated by a conserved hydrophobic motif and is spatially and temporally regulated by CENP-E. The interaction between PRC1 and microtubule motor proteins is essential for controlling chromosome partitioning, maintaining central spindle integrity, and ensuring cytokinesis during anaphase.
Article
Multidisciplinary Sciences
Man-Xiu Xie, Xian-Ying Cao, Wei-An Zeng, Ren-Chun Lai, Lan Guo, Jun-Chao Wang, Yi-Bin Xiao, Xi Zhang, Di Chen, Xian-Guo Liu, Xiao-Long Zhang
Summary: ATF4 plays a crucial role in heat nociception in mice by mediating the membrane trafficking of TRPM3, affecting the neuronal response to heat stimuli.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Jennifer M. Kunselman, Achla Gupta, Ivone Gomes, Lakshmi A. Devi, Manojkumar A. Puthenveedu
Summary: The study shows that while multiple physiological agonists can activate the same receptor, it may not necessarily be true redundancy, but rather a way to regulate downstream signaling specificity by controlling the spatiotemporal profile of signaling molecules.
Article
Oncology
Michael Guenther, Sai Agash Surendran, Michael Haas, Volker Heinemann, Michael von Bergwelt-Baildon, Jutta Engel, Jens Werner, Stefan Boeck, Steffen Ormanns
Summary: This study found that high TPX2 expression is associated with increased malignancy in PDAC and gemcitabine resistance. It suggests that high TPX2 expression can serve as a negative predictor of gemcitabine-based palliative and adjuvant chemotherapy in PDAC, providing valuable information for clinical therapy decisions.
BRITISH JOURNAL OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Hyun-Dong Cho, Nguyen Thi Thanh Nhan, Christopher Zhou, Kayeman Tu, Tara Nguyen, Nicolene A. Sarich, Kaori H. Yamada
Summary: Excessive VEGF-A signaling induces vascular leakage and angiogenesis. KIF13B-mediated recycling of internalized VEGFR2 through Rab11-positive recycling vesicle regulates endothelial permeability. Inhibition of KIF13B-mediated VEGFR2 trafficking mitigates vascular leakage in models of blinding eye disease wet AMD.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biology
Kayla Davis, Himanish Basu, Ismael Izquierdo-Villalba, Ethan Shurberg, Thomas L. Schwarz
Summary: Mitochondrial transport relies on the interaction between Miro1 and other components of the motor-adaptor complex, and the N-GTPase domain of Miro1 plays a critical role in regulating this interaction and mitochondrial motility.
LIFE SCIENCE ALLIANCE
(2022)
Article
Cell Biology
Zhiwei He, Jie Wang, Jian Xu, Xueyi Jiang, Xinyuan Liu, Jianxin Jiang
Summary: The study found that KIF15 can promote the migration and metastasis of pancreatic cancer cells. By controlling the endosome recycling of integrin beta 1, KIF15 interacts with PI3K-C2 alpha, thereby promoting the turnover of focal adhesions and distant metastasis of pancreatic cancer.
CELL DEATH & DISEASE
(2022)
Article
Virology
Nicholas Weaver, Jason Hammonds, Lingmei Ding, Grigoriy Lerner, Krista Dienger-Stambaugh, Paul Spearman
Summary: The molecular motor protein KIF16B regulates the outward sorting step involved in human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) trafficking, limiting lysosomal degradation and enhancing particle incorporation. The absence of KIF16B reduces Env expression on cell surface, leading to a decrease in Env incorporation into particles and particle infectivity. HIV-1 replication is substantially reduced in KIF16B knockout cells.
JOURNAL OF VIROLOGY
(2023)
Review
Cell Biology
Rupkatha Banerjee, Shermali Gunawardena
Summary: It has been 25 years since the discovery of phosphorylation in molecular motors, but the specific contribution of kinases to motor functions, particularly in vivo, and the evolutionary conservation of these processes, still remain largely unanswered. There is a critical need to understand how kinases regulate molecular motors due to their crucial role in intracellular processes and cell viability, as disruptions can lead to diseases such as cancer and neurodegeneration. This review focuses on the role of phosphorylation in controlling kinesin-1 motility and presents evidence for a novel regulatory mechanism involving the kinase glycogen synthase kinase 3 beta (GSK3 beta) and the scaffolding protein presenilin (PS).
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Diksha Kumari, Krishanu Ray
Summary: Phosphorylation of Kinesin subunits and adaptors plays a crucial role in regulating the activity and interactions of Kinesin motors, influencing intracellular transport dynamics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Allergy
Cyril Longe, Manuela Bratti, Mathieu Kurowska, Shamila Vibhushan, Pierre David, Valere Desmeure, Jian-Dong Huang, Alain Fischer, Genevieve de Saint Basile, Fernando E. Sepulveda, Ulrich Blank, Gael Menasche
Summary: This study demonstrates the crucial role of Rab44 in regulating secretory granule transport during degranulation in mast cells. Deficiency in Rab44 impairs granule translocation and degranulation, leading to reduced sensitivity to IgE-mediated allergic reactions. Rab44 can be considered a potential target for modulating MC degranulation and inhibiting allergic reactions.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Valerie Siahaan, Ruensern Tan, Tereza Humhalova, Lenka Libusova, Samuel E. Lacey, Tracy Tan, Mariah Dacy, Kassandra M. Ori-McKenney, Richard J. McKenney, Marcus Braun, Zdenek Lansky
Summary: Tau and MAP2 can form envelopes on microtubules by altering lattice spacing, providing a molecular basis for spatial regulation of microtubule-based processes. Envelopes can affect the activity of other MAPs and motor proteins on microtubules.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Laura Schnoeder, Inge Tomic, Laura Schwindt, Dominic Helm, Mandy Rettel, Walter Schulz-Schaeffer, Elmar Krause, Jens Rettig, Klaus Fassbender, Yang Liu
Summary: Deficiency of p38 alpha-MAPK in neurons leads to increased retrograde transportation of BACE1 in axons, shifting it from axonal terminals to lysosomes in the cell body, which exacerbates amyloid deposition in the brain of Alzheimer's disease.
Review
Biochemistry & Molecular Biology
Lejia Xu, Yuki Nagai, Yotaro Kajihara, Genta Ito, Taisuke Tomita
Summary: Rab proteins can be regulated by phosphorylation, which affects their interactions with regulatory proteins and effector proteins, thus influencing intracellular vesicle trafficking. This phosphorylation-dependent regulation plays a crucial role in determining the physiological and pathological roles of Rab proteins.
Article
Immunology
Eva Poveda, Andres Tabernilla, Wendy Fitzgerald, Angel Salgado-Barreira, Marta Grandal, Alexandre Perez, Ana Marino, Hortensia Alvarez, Nieves Valcarce, Juan Gonzalez-Garcia, Jose Ignacio Bernardino, Felix Gutierrez, Hisashi Fujioka, Manuel Crespo, Ezequiel Ruiz-Mateos, Leonid Margolis, Michael M. Lederman, Michael L. Freeman
Summary: A massive release of platelet-derived microvesicles occurs during HIV infection, some of which contain mitochondria. However, the proportion and mitochondrial density of these microvesicles are lower in HIV infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Microbiology
Emilio Bouza, Jose Ramon Arribas, Belen Alejos, Jose Ignacio Bernardino, Maye Coiras, Pep Coll, Jorge Del Romero, Maria Jose Fuster, Miguel Gorgolas, Alipio Gutierrez, Diego Gracia, Victoria Hernando, Javier Martinez-Picado, Jose Manuel Martinez Sesmero, Esteban Martinez, Santiago Moreno, Beatriz Mothe, Maria Luisa Navarro, Daniel Podzamczer, Federico Pulido, Jose Tomas Ramos, Ezequiel Ruiz-Mateos, Ines Suarez Garcia, Esteban Palomo
Summary: This article discusses the future, challenges, and prospects of HIV infection, emphasizing the importance of reflection and going beyond technical approaches.
REVISTA ESPANOLA DE QUIMIOTERAPIA
(2022)
Article
Microbiology
Helena Codina, Irene Vieitez, Alicia Gutierrez-Valencia, Vasso Skouridou, Cristina Martinez, Lucia Patino, Mariluz Botero-Gallego, Maria Trujillo-Rodriguez, Ana Serna-Gallego, Esperanza Munoz-Muela, Maria M. Bobillo, Alexandre Perez, Jorge Julio Cabrera-Alvar, Manuel Crespo, Ciara K. O'Sullivan, Ezequiel Ruiz-Mateos, Eva Poveda
Summary: The kinetics of viral and host immune response during acute COVID-19 and after remission of symptoms require further characterization. Research has found that higher levels of antibodies and cytokines early on are associated with COVID-19 severity.
Article
Microbiology
Hortensia Alvarez, Ezequiel Ruiz-Mateos, Pedro Miguel Juiz-Gonzalez, Joana Vitalle, Irene Vieitez, Maria Del Carmen Vazquez-Friol, Isabel Torres-Beceiro, Alberto Perez-Gomez, Pilar Gallego-Garcia, Nuria Estevez-Gomez, Loretta De Chiara, Eva Poveda, David Posada, Josep M. Llibre
Summary: This study reports a severely immunosuppressed individual with HIV-1/SARS-CoV-2 co-infection and a long-term course of SARS-CoV-2 infection. Despite the presence of SARS-CoV-2 variants, adaptive intra-host evolution was not identified. Spike-specific T-cell response protected against severe COVID-19, while increased immunological exhaustion markers may have favored SARS-CoV-2 persistence.
Article
Oncology
Laia Reverte, Elena Yeregui, Montserrat Olona, Alicia Gutierrez-Valencia, Maria Jose Buzon, Anna Marti, Frederic Gomez-Bertomeu, Teresa Auguet, Luis F. Lopez-Cortes, Joaquin Burgos, Clara Benavent-Bofill, Carme Boque, Graciano Garcia-Pardo, Ezequiel Ruiz-Mateos, Maria Teresa Mestre, Francesc Vidal, Consuelo Vilades, Joaquim Peraire, Anna Rull
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Esperanza Munoz-Muela, Maria Trujillo-Rodriguez, Ana Serna-Gallego, Ezequiel Ruiz-Mateos, Nuria Espinosa, Critina Roca-Oporto, Mohamed Rafii-El-Idrissi Benhnia, Luis F. Lopez-Cortes, Alicia Gutierrez-Valencia
Summary: This study aimed to evaluate the role of anti-CD4 IgG in the poor immune recovery of immunological non-responder people with HIV (INR). The results suggest that autologous anti-CD4 IgG does not trigger CD4(+) T-cell death by ADCC and is not responsible for CD4(+) lymphocyte depletion in INR.
Article
Immunology
Jenifer Masip, Norma Rallon, Elena Yeregui, Montserrat Olona, Salvador Resino, Jose M. Benito, Consuelo Vilades, Graciano Garcia-Pardo, Jose Alcami, Ezequiel Ruiz-Mateos, Frederic Gomez-Bertomeu, Montserrat Vargas, Marta Navarro, Jose A. Oteo, Juan A. Pineda, Anna Marti, Veronica Alba, Francesc Vidal, Joaquin Peraire, Anna Rull
Summary: Long-term elite controllers exhibit elevated tricarboxylic acid (TCA) cycle function and alpha-ketoglutaric acid is identified as a key metabolite in persistent control. Conversely, lipidomics analysis suggests that an elevated lipidomics plasma profile could predict the reignition of viral replication in LTEC individuals.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Alberto Perez-Gomez, Carmen Gasca-Capote, Joana Vitalle, Francisco J. Ostos, Ana Serna-Gallego, Maria Trujillo-Rodriguez, Esperanza Munoz-Muela, Teresa Giraldez-Perez, Julia Praena-Segovia, Maria D. Navarro-Amuedo, Maria Paniagua-Garcia, Manuel Garcia-Gutierrez, Manuela Aguilar-Guisado, Inmaculada Rivas-Jeremias, Maria Reyes Jimenez-Leon, Sara Bachiller, Alberto Fernandez-Villar, Alexandre Perez-Gonzalez, Alicia Gutierrez-Valencia, Mohammed Rafii-El-Idrissi Benhnia, Daniela Weiskopf, Alessandro Sette, Luis F. Lopez-Cortes, Eva Poveda, Ezequiel Ruiz-Mateos
Summary: The quality of SARS-CoV-2 specific T-cell response is associated with disease severity, immune memory, and response to other coronaviruses. In this study, it was found that mild disease is linked to a high polyfunctionality of T-cells biased towards IL-2 production, while severe disease is characterized by a predominance of IFN-gamma production without perforin production. Seven months after infection, both non-hospitalized and previously hospitalized patients showed robust anti-S IgG levels and specific T-cell responses. These findings have implications for protective immunity and vaccine strategies.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Maria Trujillo-Rodriguez, Esperanza Munoz-Muela, Ana Serna-Gallego, Juan Manuel Praena-Fernandez, Alberto Perez-Gomez, Carmen Gasca-Capote, Joana Vitalle, Joaquim Peraire, Zaira R. Palacios-Baena, Jorge Julio Cabrera, Ezequiel Ruiz-Mateos, Eva Poveda, Luis Eduardo Lopez-Cortes, Anna Rull, Alicia Gutierrez-Valencia, Luis Fernando Lopez-Cortes
Summary: Clinical and routine laboratory data at admission can effectively predict patients who will not worsen during the first two weeks, allowing for early discharge and alleviating hospital overcrowding. The determination of pro-inflammatory cytokines moderately improves the predictive accuracy.
Article
Medicine, Research & Experimental
Joana Vitalle, Alberto Perez-Gomez, Francisco Jose Ostos, Carmen Gasca-Capote, Maria Reyes Jimenez-Leon, Sara Bachiller, Inmaculada Rivas-Jeremias, Maria Del Mar Silva-Sanchez, Anabel M. Ruiz-Mateos, Maria Angeles Martin-Sanchez, Luis Fernando Lopez-Cortes, Mohammed Rafii-El-Idrissi Benhnia, Ezequiel Ruiz-Mateos
Summary: The immune factors associated with impaired SARS-CoV-2 vaccine response in elderly people are mostly unknown. This study found that aging is related to lower antibody levels, decreased T cell response, and altered immune functionality, which may contribute to the weaker vaccine response in older individuals. Understanding these factors is important for improving vaccination strategies and developing new vaccine prototypes.
Article
Immunology
Victoria Ceperuelo-Mallafre, Laia Reverte, Joaquim Peraire, Ana Madeira, Elsa Maymo-Masip, Miguel Lopez-Dupla, Alicia Gutierrez-Valencia, Ezequiel Ruiz-Mateos, Maria Jose Buzon, Rosa Jorba, Joan Vendrell, Teresa Auguet, Montserrat Olona, Francesc Vidal, Anna Rull, Sonia Fernandez-Veledo
Summary: The immune response to SARS-CoV-2 in COVID-19 patients is driven by mitochondrial-associated mechanisms, which can disrupt glucose metabolism and extracellular signaling. This study examined mitochondrial energy-related metabolites as potential biomarkers for predicting the risk of severe COVID-19 illness. The findings suggest that circulating pyruvate levels, along with other clinical factors, can serve as independent predictors of critical COVID-19 outcomes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pediatrics
Nathalia Garrido-Torres, Lucas Cerrillos, Susana Garcia Cerro, Alberto Perez Gomez, Manuel Canal-Rivero, Beatriz de Felipe, Luis Alameda, Renata Marques Rodriguez, Sergio Anillo, Julia Praena, Cristina Duque Sanchez, Cristina Roca, Maria Paniagua, Alvaro Lopez Diaz, Rafael Romero-Garcia, Peter Olbrich, Martin de Porres Puertas Albarracin, Pablo Reguera Pozuelo, Irene Lujan Sosa, Maria Begona Moreno Duenas, Rocio Pineda Cachero, Lidia Zamudio Juan, Veronica Garcia Rumi, Mercedes Guerrero Benitez, Rosario Figueroa, Antonio Manuel Martin Rendon, Antonio Partida, Maria Isabel Rodriguez Cocho, Carmen Gallardo Trujillo, Isabel Gallego Jimenez, Sarah Garcia Spencer, Marta Gomez Verdugo, Cintia Bermejo Fernandez, Maria Perez Benito, Rafael Esteban Castillo Reina, Angela Cejudo Lopez, Candela Sanchez Tomas, Maria Angeles Chacon Gamero, Ana Rubio, Amanda Moreno Mellado, Victor Ramos Herrero, Ella Starr, Marta Gonzalez Fernandez de Palacios, Elena Garcia Victori, Antonio Pavon Delgado, Ismael Fernandez Cuervo, Alejandro Arias Ruiz, Irene Esperanza Menendez Gil, Ines Dominguez Gomez, Itziar Coca Mendoza, Rosa Ayesa-Arriola, Lourdes Fananas, Juan C. Leza, Jose M. Cisneros, Javier Sanchez Cespedes, Ezequiel Ruiz-Mateos, Benedicto Crespo-Facorro, Miguel Ruiz-Veguilla
Summary: The COVID-19 pandemic provides an important opportunity for studying the pathophysiological mechanisms of neuropsychiatric diseases. This study focuses on investigating the relationship between inflammation, brain development, and the increased risk of neuropsychiatric disorders in newborns with prenatal SARS-CoV-2 infection. The study involves monitoring pregnant women and collecting biological samples before and after childbirth. The findings from this study will contribute to our understanding of the impact of COVID-19 on maternal and newborn health.
FRONTIERS IN PEDIATRICS
(2022)
Letter
Immunology
Esperanza Munoz-Muela, Ezequiel Ruiz-Mateos, Alicia Gutierrez-Valencia, Luis F. Lopez-Cortes
Article
Immunology
Eva Poveda, Wendy Fitzgerald, Cristina Reglero, Alexandre Perez-Gonzalez, Ana Marino, Hortensia Alvarez, Nieves Valcarce, Josep Maria Llibre, Santiago Moreno Guillen, Maria Angeles Munoz Fernandez, Ezequiel Ruiz-Mateos, Leonid Margolis, Michael M. Lederman, Michael L. Freeman
Summary: Elite controllers (EC) had higher levels of plasma extracellular vesicle (EV)-associated cytokines compared to other people with HIV, and persistent controllers (PC) had higher levels than transient controllers (TC). EV-associated IL-18 could distinguish EC from ART-treated individuals, while EV-associated IL-3 and TRAIL could distinguish PC from TC. Further investigation is needed to understand the role of EV-cytokines in intercellular communication and endogenous control of HIV expression.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Maria R. Jimenez-Leon, Carmen Gasca-Capote, Laura Tarancon-Diez, Beatriz Dominguez-Molina, Macarena Lopez-Verdugo, Ryan Ritraj, Isabel Gallego, Ana I. Alvarez-Rios, Joana Vitalle, Sara Bachiller, Maria Ines Camacho-Sojo, Alberto Perez-Gomez, Nuria Espinosa, Cristina Roca-Oporto, Mohamed Rafii-El-Idrissi Benhnia, Alicia Gutierrez-Valencia, Luis F. Lopez-Cortes, Ezequiel Ruiz-Mateos
Summary: Plasmacytoid dendritic cells (pDCs) can sense viral and bacterial products through Toll-like receptor (TLR)-7 and -9, and this sensing can lead to Interferon-alpha (IFN-alpha) production and T-cell activation. Understanding the mechanisms involved in pDCs stimulation could contribute to the development of HIV-cure immunotherapeutic strategies. This study aimed to investigate the immunomodulatory effects of TLR agonist stimulations in different HIV-1 disease progression phenotypes and non-infected donors.
