Article
Biotechnology & Applied Microbiology
Barbara Kubickova, Joerg A. Schenk, Franziska Ramm, Kornelija Markuskiene, Jochen Reetz, Paul Dremsek, Paulius Lukas Tamosiunas, Laima Cepulyte, Hoai Anh Trinh, Johannes Scholz, Henry Memczak, Marc Hovestaedt, Rene Ryll, Rasa Petraityte-Burneikiene, Victor M. Corman, Anika Andersson, Dietmar Becher, Martin H. Groschup, Stefan Kubick, Frank Sellrie, Reimar Johne, Rainer G. Ulrich
Summary: A hepatitis E virus (HEV) genotype 3-specific monoclonal antibody (mAb) was successfully generated by immunising BALB/c mice with a carboxy-terminal part of its capsid protein expressed in Escherichia coli. The mAb, named G117-AA4, showed strong reactivity with homologous and heterologous HEV-3 capsid proteins, as well as with capsid protein derivatives from other genotypes and related viruses. This novel mAb may prove valuable for further studies on HEV infections and diagnostic purposes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Virology
Simon D. Lytton, Rakibul Hassan Bulbul, Kishor Barua, Mosa C. Begum, Bani Chowdhury, Zahirul Islam, Kazi Ishrak Faiyaz, Shahnawaz Khan Chandan, Ahmed Shakeel, Olfert Landt, Rashid Mamunur
Summary: In an HEV outbreak in Chittagong, Bangladesh, the HEV-Ag ELISA proved to be a reliable and practical diagnostic tool for acute HEV infections, showing higher positivity in viral RNA-positive cases and a positive correlation with viremia.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Microbiology
Mengnan Fan, Yuhang Luo, Beibei Zhang, Jiaxi Wang, Tianxiang Chen, Baoyuan Liu, Yani Sun, Yuchen Nan, Julian A. Hiscox, Qin Zhao, En-Min Zhou
Summary: The study confirmed the direct interaction between HEV and CDC42, and identified different CDC42 downstream signaling pathways used by non-enveloped and quasi-enveloped HEV during infection. These results provide new insights into the life cycle of HEV in host cells and offer a new drug design target for combating HEV infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Cell Biology
Daniela Lenggenhager, Jonas Grossmann, Jerome Gouttenoire, Christine Sempoux, Achim Weber
Summary: Immunohistochemistry for HEV ORF2 protein is a useful tool for tissue-based diagnosis of hepatitis E. However, we report an unexpected immunoreactivity of the HEV ORF2 antibody to CMV proteins. The staining pattern of CMV-infected cells is limited to the nucleus, in contrast to the cytoplasmic and occasional canalicular positivity observed in hepatitis E.
Review
Microbiology
Zhaobin Zhou, Yinqian Xie, Chunyan Wu, Yuchen Nan
Summary: Hepatitis E virus (HEV) is a zoonotic pathogen responsible for acute hepatitis outbreaks globally. The HEV-ORF2 protein, in addition to forming the viral capsid, plays multiple roles in viral replication and pathogenesis, making it a potential target for vaccine development and treatment of HEV infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Bo Wang, Debin Tian, Harini Sooryanarain, Hassan M. Mahsoub, C. Lynn Heffron, Anna M. Hassebroek, Xiang-Jin Meng
Summary: Hepatitis E virus (HEV) infection in pregnant women often leads to fulminant hepatic failure (FHF) with high mortality rates. Mutations in HEV-1 have been found to be associated with FHF, and two specific mutations have been identified in all FHF cases in Bangladesh. These mutations greatly enhance HEV replication and may explain the rapid viral replication and severe disease in FHF patients. However, these mutations do not affect the replication ability of HEV-3 and do not alter the sensitivity to ribavirin, suggesting that ribavirin can still be used for antiviral therapy in FHF patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Elitsa Golkocheva-Markova
Summary: Understanding the molecular mechanism of hepatitis E virus (HEV) is crucial for revealing the pathogenesis of the virus.
Article
Microbiology
Hassan M. M. Mahsoub, C. Lynn Heffron, Anna M. M. Hassebroek, Harini Sooryanarain, Bo Wang, Tanya LeRoith, Guillermo Raimundi Rodriguez, Debin Tian, Xiang-Jin Meng
Summary: HEV causes adverse pregnancy outcomes with a mortality rate of >30% in pregnant women. However, the underlying mechanisms are poorly understood. This study utilizes HEV-3ra and pregnant rabbits to investigate the potential mechanisms of pregnancy-associated adverse outcomes during HEV infection.
Article
Biology
Yakubu Egigogo Raji, Ooi Peck Toung, Niazlin Mohd Taib, Zamberi Bin Sekawi
Summary: HEV is a global public health and economic challenge affecting both humans and animals worldwide. Recent studies suggest endemicity of HEV in both developing and industrialized nations. The virus has diverse transmission routes and potential risk factors.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Virology
Gergana Zahmanova, Katerina Takova, Valeria Tonova, Tsvetoslav Koynarski, Laura L. Lukov, Ivan Minkov, Maria Pishmisheva, Stanislav Kotsev, Ilia Tsachev, Magdalena Baymakova, Anton P. Andonov
Summary: Hepatitis E virus (HEV) is a major cause of acute viral hepatitis transmitted mainly through ingestion of contaminated water or food, or contact with infected animals and their raw meat products. HEV is a re-emerging virus in developed countries, posing a risk to public health. Preventative efforts and further research on HEV vaccine development are needed to reduce the incidence of HEV infection and its associated complications.
Article
Immunology
Cassandra C. Lautredou, Bonnie Dao, Prabhu Gounder
Summary: In Los Angeles County, 38 out of 48 individuals who tested positive for hepatitis E virus (HEV) immunoglobulin M did not meet the criteria for acute HEV cases during a 3-year period. Healthcare providers should limit HEV serologic testing to individuals with clinically compatible symptoms or epidemiologic risk factors.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Bo Wang, Hassan M. M. Mahsoub, Wen Li, C. Lynn Heffron, Debin Tian, Anna M. M. Hassebroek, Tanya LeRoith, Xiang-Jin Meng
Summary: HEV-3 causes chronic hepatitis E that requires antiviral therapy in immunosuppressed individuals, and ribavirin (RBV) is the main therapeutic option, although it is an off-label use. Treatment failure may occur due to mutations in the viral RNA-dependent RNA polymerase (RdRp), including Y1320H, K1383N, and G1634R.
Article
Gastroenterology & Hepatology
Patrick Behrendt, Birgit Bremer, Daniel Todt, Eike Steinmann, Michael Peter Manns, Markus Cornberg, Heiner Wedemeyer, Benjamin Maasoumy
Summary: Different methods for RNA extraction and quantification have a significant, compartment-specific impact on the sensitivity of HEV detection. Knowledge about the favourable combinations of extraction and quantification has important implications for diagnosis and patients receiving antiviral therapy.
LIVER INTERNATIONAL
(2021)
Article
Virology
Giulia Pezzoni, Lidia Stercoli, Eleonora Pegoiani, Emiliana Brocchi
Summary: In the study evaluating the antigenic properties of HEV ORF2 proteins, 37 MAbs were raised, with one antigenic region competing with positive sera and efficiently recognizing the recombinant antigen. The ORF2 portion from 394-608 aa contains most immunogenic epitopes, with 85% of positive sera reacting to the region from 461-544 aa, while only 5% of selected sera reacted to the ORF3 antigen.
Article
Chemistry, Multidisciplinary
Hyun Woo Won
Summary: The performance of hybrid electric vehicles heavily relies on subsystem components and their architecture, necessitating comprehensive reviews before manufacturing. Designers can utilize simulations to develop virtual prototypes and quickly assess design modifications without the need for costly physical prototypes. Control strategies and tools such as computational modeling and optimization are essential for achieving emission and hardware cost targets. The author's rule-based hybrid simulation tool offers enhanced flexibility for users to select different control strategies and explore a variety of hybrid topologies, providing the ability to modify subsystems as needed.
APPLIED SCIENCES-BASEL
(2021)
Article
Pathology
Kent Lewandrowski, Alissa Keegan, Vladislav Makarenko, Elena Maryamchak, Douglas A. Mata, Michael J. Palte, Joseph W. Rudolf, Nida Safdar, Ryan J. Schmidt, Lena Stuart, Yiqin Xiong, Lokinendi Rao, Stacy E. F. Melanson, L. Michael Snyder
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2018)
Article
Biology
Alan M. West, Scott C. Rosenbereg, Sarah N. Ur, Madison K. Lehmern, Qiaozhen Ye, Goetz Hagemann, Iracema Caballero, Isabel Uson, Amy J. MacQueen, Franz Herzog, Kevin D. Corbett
Editorial Material
Urology & Nephrology
Julie A. Szymaniak, Douglas A. Mata, Alexander P. Cole
Article
Neurosciences
Kathryn K. Ridout, Samuel J. Ridout, Constance Guille, Douglas A. Mata, Huda Akil, Srijan Sen
BIOLOGICAL PSYCHIATRY
(2019)
Article
Biochemistry & Molecular Biology
Rebecca K. Lau, Qiaozhen Ye, Erica A. Birkholz, Kyle R. Berg, Lucas Patel, Ian T. Mathews, Jeramie D. Watrous, Kaori Ego, Aaron T. Whiteley, Brianna Lowey, John J. Mekalanos, Philip J. Kranzusch, Mohit Jain, Joe Pogliano, Kevin D. Corbett
Article
Biochemistry & Molecular Biology
Qiaozhen Ye, Rebecca K. Lau, Ian T. Mathews, Erica A. Birkholz, Jeramie D. Watrous, Camillia S. Azimi, Joe Pogliano, Mohit Jain, Kevin D. Corbett
Article
Biochemistry & Molecular Biology
Qiaozhen Ye, Alan M. V. West, Steve Silletti, Kevin D. Corbett
Article
Multidisciplinary Sciences
Shan Lu, Qiaozhen Ye, Digvijay Singh, Yong Cao, Jolene K. Diedrich, John R. Yates, Elizabeth Villa, Don W. Cleveland, Kevin D. Corbett
Summary: The SARS-CoV-2 nucleocapsid (N) protein binds viral RNA and undergoes liquid-liquid phase separation driven by its central intrinsically-disordered region, modulated by phosphorylation. Additionally, the N protein interacts with the membrane protein M to form mutually exclusive compartments in a three-component mixture with RNA.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Qiaozhen Ye, Shan Lu, Kevin D. Corbett
Summary: The COVID-19 pandemic caused by SARS-CoV-2 is a severe public health event, requiring rapid and accurate diagnostic methods. Antigen tests, especially those detecting the SARS-CoV-2 Nucleocapsid protein, are effective in detecting active infections. Research has shown that three llama-derived single-domain antibodies have high affinity for the SARS-CoV-2 Nucleocapsid protein, offering potential for developing affordable diagnostic tests for all circulating variants of SARS-CoV-2.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chelsea L. Blankenchip, Justin Nguyen, Rebecca K. Lau, Qiaozhen Ye, Yajie Gu, Kevin D. Corbett
Summary: Bacteria utilize diverse immune systems to defend against viruses, with the transcription factor CapW associated with regulating the CBASS bacterial immune system response. CapW represses CBASS gene expression in uninfected cells and its activation is not necessary for strong anti-phage activity, suggesting it may play a role in response to signals other than phage infection. CapW is part of a family of universal defense signaling proteins, similar to the BrxR transcription factor associated with the BREX anti-phage system.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Qishan Liang, Sara T. Richey, Sarah N. Ur, Qiaozhen Ye, Rebecca K. Lau, Kevin D. Corbett
Summary: The CBASS immune systems in bacteria protect against bacteriophage infection by triggering cell death. Cap18, a 3'-5' exonuclease associated with CBASS systems, was found to have nonspecific DNA exonuclease activity and may coordinate with transcription factors to regulate CBASS transcription in response to DNA damage.
Article
Cell Biology
Shan Lu, Jiaojiao Hu, Olubankole Aladesuyi Arogundade, Alexander Goginashvili, Sonia Vazquez-Sanchez, Jolene K. Diedrich, Jinge Gu, Jacob Blum, Spencer Oung, Qiaozhen Ye, Haiyang Yu, John Ravits, Cong Liu, John R. Yates, Don W. Cleveland
Summary: This study identifies HSPB1 as a regulator of cytoplasmic TDP-43 phase separation and aggregation, and suggests that decreased HSPB1 may be involved in the pathogenesis of ALS and other neurodegenerative diseases.
NATURE CELL BIOLOGY
(2022)
Article
Oncology
Michael Michal, Oleksandr Kravtsov, Jeffrey S. Ross, Daniela Skanderova, Petr Martinek, Elaheh Mosaieby, Douglas A. Mata, Erik A. Williams, Yin P. Hung
Summary: In this study, a patient with clear cell mesothelioma was identified, and the presence of an inactivating VHL mutation was confirmed. Further analysis revealed a cohort of four VHL-mutant clear cell mesotheliomas with distinct clinicopathologic and molecular features. These tumors were characterized by prominent clear cells, inactivating VHL mutations, and genomic near-haploidization.
GENES CHROMOSOMES & CANCER
(2023)
Letter
Medicine, General & Internal
Douglas A. Mata, Lisa S. Rotenstein, Marco A. Ramos, Anupam B. Jena
Summary: From 2013 to 2022, there was an increase in the number of cancer patients undergoing genomic testing, with an annual increase of 0.45 percentage points in patients of African ancestry.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Neurosciences
Douglas A. Mata, Jamal K. Benhamida, Andrew L. Lin, Chad M. Vanderbilt, Soo-Ryum Yang, Liliana B. Villafania, Donna C. Ferguson, Philip Jonsson, Alexandra M. Miller, Viviane Tabar, Cameron W. Brennan, Nelson S. Moss, Martin Sill, Ryma Benayed, Ingo K. Mellinghoff, Marc K. Rosenblum, Maria E. Arcila, Marc Ladanyi, Tejus A. Bale
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2020)