Article
Cell & Tissue Engineering
Mariam F. Farid, Yara S. Abouelela, Noha A. E. Yasin, Asmaa K. Al-Mokaddem, Abdelbary Prince, Marwa A. Ibrahim, Hamdy Rizk
Summary: This study aimed to evaluate the regenerative capacity of low-level laser-activated SVF in an MS cat model. The results showed that SVF treatment significantly improved locomotion activity, BBB scores, lesion extent, and expression of neurotrophic factors. SVF treatment also led to an increase in oligodendrocyte transcription factor and myelin basic protein, and a reduction in apoptosis and axonal degeneration, demonstrating its potential for remyelination and regeneration in MS.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Immunology
Nancy E. Gomez, Victoria James, Kenton P. Arkill, Zubair A. Nizamudeen, David Onion, Lucy C. Fairclough
Summary: This study aimed to optimize methodologies to study immune cell-derived extracellular vesicles (EVs). Peripheral blood mononuclear cell-derived small EVs were isolated and characterized using conventional transmission electron microscopy and nanoparticle tracking analysis. They were further analyzed using imaging flow cytometry and flow cytometric multiplex bead assay. These techniques were applied to smoking-related inflammatory disease research following the Minimal Information for Studies of Extracellular Vesicle 2018 guidelines.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Kristian W. Antonsen, Henriette N. Friis, Boe S. Sorensen, Anders Etzerodt, Soren K. Moestrup, Holger J. Moller
Summary: The impact of culture medium composition on monocyte-derived macrophage phenotype was investigated in this study. The results showed that changes in culture medium composition affected yield, cell size, gene expression, membrane protein levels, and release of soluble proteins. The most pronounced effects were observed when culturing macrophages in DMEM, which lacks certain non-essential amino acids.
Article
Cardiac & Cardiovascular Systems
William Bracamonte-Baran, Nisha A. Gilotra, Taejoon Won, Katrina M. Rodriguez, Monica Talor, Byoung C. Oh, Jan Griffin, Ilan Wittstein, Kavita Sharma, John Skinner, Roger A. Johns, Stuart D. Russell, Robert A. Anders, Qingfeng Zhu, Marc K. Halushka, Gerald Brandacher, Daniela Cihakova
Summary: The study found that myeloid cells dominate the graft leukocyte compartment in heart transplant patients, with variable CD4:CD8 T-cell ratios. PD-L1 expression in graft endothelial cells, fibroblasts, and myeloid leukocytes varied from negligible to 60%. There was a significant inverse correlation between PD-L1(+)HLA-DR+ endothelial cells and CD8(+) T cells, and these patterns were independent of demographic, clinical, and hemodynamic parameters. Additionally, a murine model confirmed the importance of endothelial PD-L1 expression in regulating CD8(+) T-cell infiltration and potential alloresponses in heart transplantation.
CIRCULATION-HEART FAILURE
(2021)
Article
Biochemistry & Molecular Biology
Mahboobeh Nazari, Rahman Emamzadeh, Maryam Jahanpanah, Elnaz Yazdani, Ramin Radmanesh
Summary: In this study, a new affitoxin was designed and tested for its ability to target and kill HER3 positive breast cancer cells. The results showed that the affitoxin specifically bound to HER3 positive cells and had the potential to be a new treatment option for HER3-positive cancers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agronomy
Vijayakumari Narukulla, Yogesh Lahane, Krutika Fiske, Shashi Pandey, Vasileios Ziogas
Summary: In this study, stable tetraploids were developed in citrus rootstock cultivars including Rough lemon, Rangpur lime, and Alemow through in vitro colchicine treatments. The treatment with 0.1% colchicine for 24 hours resulted in the highest tetraploid induction percentage (18.3%) in all the rootstock cultivars.
Article
Zoology
David Sadilek, Jitka Vilimova, Tomas Urfus
Summary: The study analyzed genome size and the position of 18S ribosomal DNA in different species from the family Nabidae, revealing distinct chromosomal characteristics and significant differences in nuclear DNA content among the three genera studied. Notably, the results strongly support the theory of an autosomal polyploidy event in the evolution of the genus Himacerus.
ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
(2021)
Article
Biology
Anna Leabourn Boss, Tanvi Damani, Tayla J. Wickman, Larry W. Chamley, Joanna L. James, Anna E. S. Brooks
Summary: Researchers used flow cytometry to phenotypically characterize mesenchymal cells in the placenta and identified four distinct subsets. They also discovered that some mesenchymal cells undergo phenotypic convergence in ex-vivo culture. This study highlights the importance of detailed cell phenotyping for optimizing therapeutic capacity and ensuring the use of relevant cells in functional assays.
Article
Agriculture, Dairy & Animal Science
Hanan Hendawy, Akiko Uemura, Danfu Ma, Ryosuke Namiki, Haney Samir, Mahmoud F. Ahmed, Ahmed Elfadadny, Hussein M. El-Husseiny, Cheng Chieh-Jen, Ryou Tanaka
Summary: The study addressed the ideal adipose tissue harvesting site for obtaining high-quality stromal vascular fraction (SVF) cells in middle-aged to old dogs. The peri-ovarian region was found to yield the highest viability and cellular yield of SVF cells with greater expression of adipose-derived mesenchymal stem cell markers, making it the preferred AT sampling site for canine regenerative therapies.
Article
Biotechnology & Applied Microbiology
Lucas C. Olson, James T. Redden, LaStar Gilliam, Tri M. Nguyen, Josephina A. Vossen, David J. Cohen, Zvi Schwartz, Michael J. McClure
Summary: This study investigates the increased levels of AGEs and RAGE post-trauma and the effects of different treatment methods on muscle regeneration. The results show that the combination of ASCs and DMM treatment improves muscle regeneration and regulates the RAGE and p38 MAPK signaling pathways.
BIOENGINEERING-BASEL
(2022)
Article
Cell Biology
Stelios Chrysostomou, Rajat Roy, Filippo Prischi, Lucksamon Thamlikitkul, Kathryn L. Chapman, Uwais Mufti, Robert Peach, Laifeng Ding, David Hancock, Christopher Moore, Miriam Molina-Arcas, Francesco Mauri, David J. Pinato, Joel M. Abrahams, Silvia Ottaviani, Leandro Castellano, Georgios Giamas, Jennifer Pascoe, Devmini Moonamale, Sarah Pirrie, Claire Gaunt, Lucinda Billingham, Neil M. Steven, Michael Cullen, David Hrouda, Mathias Winkler, John Post, Philip Cohen, Seth J. Salpeter, Vered Bar, Adi Zundelevich, Shay Golan, Dan Leibovici, Romain Lara, David R. Klug, Sophia N. Yaliraki, Mauricio Barahona, Yulan Wang, Julian Downward, J. Mark Skehel, Maruf M. U. Ali, Michael J. Seckl, Olivier E. Pardo
Summary: The study identified RSK4 as a driver of drug resistance and metastasis in lung and bladder cancer cells, and silencing this kinase increased sensitivity to chemotherapy and reduced metastasis. Drug screening revealed fluoroquinolone antibiotics as potent RSK4 inhibitors, with trovafloxacin replicating effects of RSK4 silencing. Further structural analysis provided insights into how trovafloxacin blocks RSK4 kinase activation. Additionally, patients receiving chemotherapy and prophylactic levofloxacin showed significantly prolonged overall survival, suggesting RSK4 inhibition as a potential therapeutic strategy for lung and bladder cancer.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Hye Won Lee, Hyunwoo Lee, Chanho Park, Won Joon Oh, Tae Jin Kim, Ghee Young Kwon, Seong Il Seo
Summary: The study presents the immune microenvironment pattern of MESTK to oncogenic stress, revealing the distribution and characteristics of TILs in the tumor stroma. High levels of effector memory T cells were found in MESTK samples, along with other activated B cell populations.
Article
Materials Science, Multidisciplinary
Sareh Zhand, Kun Xiao, Sajad Razavi Bazaz, Ying Zhu, Pritam Bordhan, Dayong Jin, Majid Ebrahimi Warkiani
Summary: Extracellular vesicles, particularly exosomes, are valuable tools for analyzing physiological processes and serving as alternative sources of disease diagnostic and prognostic biomarkers in liquid biopsies.
APPLIED MATERIALS TODAY
(2021)
Article
Biotechnology & Applied Microbiology
Denise dos Santos Vila Verde, Maria Ines de Souza Mendes, Antonio da Silva Souza, Fabiana Ferraz Aud, Karen Cristina Fialho dos Santos, Walter dos Santos Soares Filho, Abelmon da Silva Gesteira, Marcio Gilberto Cardoso Costa
Summary: The aim of this study was to use flow cytometry analyses to identify polyploid individuals among tangerine-type seedlings, in order to obtain seedless fruits. After seeds were collected from 13 different genotypes, they were categorized into developed, underdeveloped, and small. Polyploids were found in the underdeveloped and small seeds, with a haploid plant also obtained.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Biochemistry & Molecular Biology
Rimma Sergeevna Kamentseva, Marianna Viktorovna Kharchenko, Gulnara Vladikovna Gabdrahmanova, Michael Alexandrovich Kotov, Vera Vladislavovna Kosheverova, Elena Sergeevna Kornilova
Summary: This study compared the effects of EGF, TGF-α, and AREG on physiological responses in endometrial MSCs and HeLa cells, and investigated the internalization and degradation of EGFR stimulated by these ligands. The study found that while EGF and TGF-α stimulated cell proliferation and prevented differentiation in enMSCs in an EGFR-dependent manner, AREG had no effect. Additionally, differences were observed in the degradation and recovery of EGFR in different cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Transplantation
Carol Conrad
CURRENT OPINION IN ORGAN TRANSPLANTATION
(2020)
Review
Pediatrics
Archana Chacko, Richard B. Moss
CURRENT OPINION IN PEDIATRICS
(2020)
Editorial Material
Medicine, General & Internal
Richard B. Moss
Review
Allergy
Brian C. M. Li, Sung Moon Huh, Miguel D. Prieto, Gina Hong, Carsten Schwarz, Richard B. Moss, Bradley S. Quon
Summary: Diagnosing ABPA in CF patients can be challenging, but recent studies have identified promising biomarkers that may aid in diagnosis. Further research is needed to understand their utility better.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2021)
Article
Surgery
Sarah Duncan-Park, Claire Dunphy, Jacqueline Becker, Christine D'Urso, Rachel Annunziato, Joshua Blatter, Carol Conrad, Samuel B. Goldfarb, Don Hayes, Ernestina Melicoff, Marc Schecter, Gary Visner, Brian Armstrong, Hyunsook Chin, Karen Kesler, Nikki M. Williams, Jonah N. Odim, Stuart C. Sweet, Lara Danziger-Isakov, Eyal Shemesh
Summary: The study found that remote interventions in pediatric lung transplant recipients can effectively reduce medication level variability, improve blood level stability, and participants successfully completed long-term remote interventions.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Editorial Material
Allergy
Richard B. Moss
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Respiratory System
Gina Hong, Sameer Desai, Richard B. Moss, Patience Eschenhagen, Bradley S. Quon, Carsten Schwarz
Summary: There are significant variations in the diagnosis and management of Aspergillus-related conditions in CF. Future studies are needed to better harmonize the approach to Af-related disease in CF.
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Surgery
Stuart C. Sweet, Brian Armstrong, Joshua Blatter, Hyunsook Chin, Carol Conrad, Samuel Goldfarb, Don Hayes, Peter S. Heeger, Victoria Lyou, Ernestina Melicoff-Portillo, Thalachallour Mohanakumar, Jonah Odim, Ranjithkumar Ravichandran, Marc Schecter, Gregory A. Storch, Gary Visner, Nikki M. Williams, Lara Danziger-Isakov
Summary: The study showed that rituximab induction may prevent early DSA development in pediatric lung transplant recipients without adverse effects, but did not significantly differ from the placebo group in terms of the primary endpoint and incidence of adverse events.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Pediatrics
Carol K. Conrad, Haley Hedlin, Hyunsook Chin, Don Hayes, Peter S. Heeger, Albert Faro, Samuel Goldfarb, Ernestina Melicoff-Portillo, Mohanakumar Thalachallour, Jonah Odim, Marc Schecter, Gregory A. Storch, Gary A. Visner, Nikki M. Williams, Karen Kesler, Lara Danziger-Isakov, Stuart C. Sweet
Summary: Cytokine analysis of bronchoalveolar lavage fluid samples from pediatric lung transplant recipients revealed that elevated levels of IL-23 and IL-31 were associated with an increased risk of bronchiolitis obliterans, while lower levels of epithelial growth factor and eotaxin were associated with bronchiolitis obliterans. Furthermore, soluble CD30, pro-inflammatory cytokine IL-23, and sTNFRI levels below cutoff levels were found to be associated with bronchiolitis obliterans-free survival.
PEDIATRIC TRANSPLANTATION
(2022)
Article
Microbiology
Efthymia Iliana Matthaiou, Wayland Chiu, Carol Conrad, Joe Hsu
Summary: Cystic fibrosis lung transplant recipients are at a higher risk of life-threatening invasive aspergillosis. Alkalization of macrophages lacking the CFTR gene is associated with this increased risk, and the presence of iron in the post-transplant microenvironment exacerbates this alkalization. The alkalization impairs the ability of macrophages to clear Aspergillus fumigatus conidia. pH modulation after transplantation may be a therapeutic strategy to reduce the risk of invasive aspergillosis.
Article
Respiratory System
Gregory S. Sawicki, Michael W. Konstan, Edward F. McKone, Richard B. Moss, Barry Lubarsky, Ellison Suthoff, Stefanie J. Millar, David J. Pasta, Nicole Mayer-Hamblett, Christopher H. Goss, Wayne J. Morgan, Margaret E. Duncan, Yoojung Yang
Summary: This study examined the rate of lung function decline in cystic fibrosis patients with F/RF genotypes. The results showed that lung function declines over time in patients of all ages with F/RF genotypes. This finding emphasizes the importance of early intervention and clinical monitoring to preserve lung function in all cystic fibrosis patients.
Article
Microbiology
Richard B. B. Moss
Summary: Allergic asthma has traditionally been treated with inhaled and systemic glucocorticosteroids. A continuum of allergic fungal airways disease associated with Aspergillus fumigatus colonization and/or atopic immune responses that encompasses fungal asthma, severe asthma with fungal sensitization and allergic bronchopulmonary aspergillosis is now recognized along a phenotypic severity spectrum of T2-high immune deviation lung disease. Oral triazoles have shown clinical, anti-inflammatory and microbiologic efficacy in this setting; in the future inhaled antifungals may improve the therapeutic index. Humanized monoclonal antibody biologic agents targeting T2-high disease also show efficacy and promise of improved control in difficult cases. Developments in these areas are highlighted in this overview.
Article
Immunology
Joseph E. Levitt, Haley Hedlin, Sophie Duong, Di Lu, Justin Lee, Bryan Bunning, Nadia Elkarra, Benjamin A. Pinsky, Eileen Heffernan, Eric Springman, Richard B. Moss, Hector F. Bonilla, Julie Parsonnet, Roham T. Zamanian, Jamison J. Langguth, Jenna Bollyky, Chaitan Khosla, Mark R. Nicolls, Manisha Desai, Angela J. Rogers
Summary: The study aimed to evaluate the effectiveness of Acebilustat in treating outpatients with COVID-19, and the results showed that the medication did not shorten symptom duration.
CLINICAL INFECTIOUS DISEASES
(2023)
Correction
Microbiology
Richard B. B. Moss
Article
Pediatrics
Rebekah H. Nevel, Gail Deutsch, Daniel Craven, Robin P. Deterding, Martha A. Fishman, Jennifer Wambach, Alicia Casey, Katie R. Krone, Deborah G. Liptzin, Michael O'Connor, Geoffrey B. Kurland, Jane A. Taylor, William S. Gower, James Hagood, Carol B. Conrad, Jade K. Tam-Williams, Elizabeth Fiorino, Samuel C. Goldfarb, Sara M. Sadreameli, Lawrence Nogee, Gregory Montgomery, Aaron A. Hamvas, Theresa Laguna, Manvi Bansal, Cheryl Lew, Maria Santiago, Antonia Popova, Aliva De, Marilynn R. Chan, Michael B. Powers, Maureen Josephson, Devaney Camburn, Laura Voss, Yun R. Li, Lisa Young
Summary: Childhood interstitial and diffuse lung disease (chILD) encompasses a broad spectrum of rare disorders. The Children's Interstitial and Diffuse Lung Disease Research Network (chILDRN) established a prospective registry to advance knowledge regarding these disorders.
PEDIATRIC PULMONOLOGY
(2023)