期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 27, 页码 11336-11341出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0813160106
关键词
adult neurogenesis; coupling; gap junction; astrocyte; patch clamp
资金
- Deutsche Forschungsgemeinschaft [SFB/TR3, C1, C9, N01]
- SFB 645 [B2, SPP1172, SE 774/3]
- European Commission [FP7-202167NeuroGLIA]
- Forschungskommission der Medizinischen Fakultat Universitatsklinikum Bonn
- Interdisziplinares Zentrum fur Klinische Forschung Jena 1.7
In the adult dentate gyrus, radial glia-like cells represent putative stem cells generating neurons and glial cells. Here, we combined patch-clamp recordings, biocytin filling, immunohistochemistry, single-cell transcript analysis, and mouse transgenics to test for connexin expression and gap junctional coupling of radial glia-like cells and its impact on neurogenesis. Radial glia-like cells were identified in mice expressing EGFP under control of the nestin and gfap promoters. We show that a majority of Radial glia-like cells are coupled and express Cx43. Neuronal precursors were not coupled. Mice lacking Cx30 and Cx43 in GFAP-positive cells displayed almost complete inhibition of proliferation and a significant decline in numbers of radial glia-like cells and granule neurons. Inducible virus-mediated ablation of connexins in the adult hippocampus also reduced neurogenesis. These findings strongly suggest the requirement of connexin expression by radial glia-like cells for intact neurogenesis in the adult brain and point to possible communication pathways of these cells.
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