Article
Cell Biology
Fernande Ayenoue Siadous, Franck Cantet, Erin Van Schaik, Melanie Burette, Julie Allombert, Anissa Lakhani, Boris Bonaventure, Caroline Goujon, James Samuel, Matteo Bonazzi, Eric Martinez
Summary: The Coxiella burnetii effector protein CvpF interacts with host small GTPase RAB26, leading to the recruitment of autophagosomal marker MAP1LC3B/LC3B to Coxiella-containing vacuoles, affecting the pathogen's virulence.
Article
Multidisciplinary Sciences
Yunjia Shi, Hongtao Liu, Kelong Ma, Zhao-Qing Luo, Jiazhang Qiu
Summary: Legionella organisms are environmental bacteria that cause Legionnaires' disease. A study characterized an effector protein, RavZ, in L. longbeachae, the second leading cause of Legionella infections. RavZ inhibits autophagy signaling in host cells challenged with L. pneumophila and L. longbeachae, indicating functional redundancy between RavZ(LLO) and RavZ(LP). RavZ(LLO) also reduces cellular levels of polyubiquitinated and polyneddylated proteins. RavZ(LLO) plays a role in the regulation of polyubiquitinated species on the Legionella-containing vacuole during L. longbeachae infection.
Article
Biochemistry & Molecular Biology
Yuta Homma, Mitsunori Fukuda
Summary: The study identified VPS52 as an effector protein responsible for Rab6-dependent secretory cargo trafficking, with its knockout resulting in attenuated secretion and lysosomal accumulation of secretory cargos.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biology
Pooja Pandey, Alexandre Y. Leary, Yasin Tumtas, Zachary Savage, Bayantes Dagvadorj, Cian Duggan, Enoch L. H. Yuen, Nattapong Sanguankiattichai, Emily Tan, Virendrasinh Khandare, Amber J. Connerton, Temur Yunusov, Mathias Madalinski, Federico Gabriel Mirkin, Sebastian Schornack, Yasin Dagdas, Sophien Kamoun, Tolga O. Bozkurt
Summary: The effector protein PexRD54 secreted by the Irish famine pathogen Phytophthora infestans can induce autophagosome formation and promote the generation of autophagosomes by utilizing lipid droplets through specific trafficking pathways. This effector protein mimics starvation-induced autophagy to alter endomembrane trafficking at the host-pathogen interface, facilitating colonization by the pathogen.
Article
Biochemistry & Molecular Biology
Noah J. Harris, Meredith L. Jenkins, Udit Dalwadi, Kaelin D. Fleming, Sung-Eun Nam, Matthew A. H. Parson, Calvin K. Yip, John E. Burke
Summary: Transport Protein Particle complexes (TRAPP) are highly conserved regulators of membrane trafficking, with distinct TRAPPII and TRAPPIII complexes showing specificity towards different Rab GTPases. Biochemical assays, HDX-MS, and electron microscopy were used to study the regulation of TRAPPII and TRAPPIII complexes, revealing differences in GEF activity, protein dynamics, and membrane association. The results provide insights into the functions of TRAPP complexes and their mechanisms of achieving Rab specificity.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Plant Sciences
Tianli Li, Gan Ai, Xiaowei Fu, Jin Liu, Hai Zhu, Ying Zhai, Weiye Pan, Danyu Shen, Maofeng Jing, Ai Xia, Daolong Dou
Summary: This study characterizes the functional properties of the core RXLR effector RXLR242 in P. capsici and its manipulation of host plant immunity. RXLR242 interacts with RAB proteins and disrupts protein trafficking, thereby promoting Phytophthora infection.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Microbiology
Karsten Meier, Lana H. Jachmann, Goezde Tuerkoez, Mohammed Rizwan Babu Sait, Lucia Perez, Oliver Kepp, Raphael H. Valdivia, Guido Kroemer, Barbara S. Sixt
Summary: This study revealed the key role of CpoS protein from Chlamydia trachomatis in the modulation of inclusion membrane and membrane trafficking, as well as its ability to suppress interferon response through interaction with Rab GTPases. These findings advance our understanding of how intracellular bacteria shape and protect their growth niche.
Review
Cell Biology
Xue Huang, Jia Yao, Lu Liu, Yu Luo, Aimin Yang
Summary: Autophagy is an intracellular degradation pathway that maintains cellular homeostasis. The use of autophagy marker Atg8-PE protein-based methods has greatly contributed to the understanding of autophagy-related mechanisms. Recent advances in biochemical approaches have successfully produced lipidated Atg8-PE protein or its mimics in vitro, providing insights into membrane dynamics and protein-protein interactions.
Article
Cell Biology
Jorge Eduardo Del-Rio-Robles, Janik Adriana Tomas-Morales, Cesar Zavala-Barrera, Alejandro Castillo-Kauil, Irving Garcia-Jimenez, Jose Vazquez-Prado, Guadalupe Reyes-Cruz
Summary: The calcium sensing receptor (CaSR) activates the Rab11A-dependent pathway to promote Rab27B-regulated secretion, which is involved in the secretion of chemotactic and inflammatory factors.
CELLULAR SIGNALLING
(2023)
Article
Biology
Hongki Song, Thomas L. Torng, Amy S. Orr, Axel T. Brunger, William T. Wickner
Summary: Membrane fusion requires SNARE proteins to form coiled coils, but Sec17 and Sec18 can promote fusion without the need for completion of the coiled coils. Specific blocks in SNARE proteins can be bypassed by Sec17 and Sec18 to restore fusion.
Article
Medicine, General & Internal
Monica S. Thakar, Brent R. Logan, Jennifer M. Puck, Elizabeth A. Dunn, Rebecca H. Buckley, Morton J. Cowan, Richard J. O'Reilly, Neena Kapoor, Lisa Forbes Satter, Sung-Yun Pai, Jennifer Heimall, Sharat Chandra, Christen L. Ebens, Deepak Chellapandian, Olatundun Williams, Lauri M. Burroughs, Blachy Davila Saldana, Ahmad Rayes, Lisa M. Madden, Shanmuganathan Chandrakasan, Jeffrey J. Bednarski, Kenneth B. Desantes, Geoffrey D. E. Cuvelier, Pierre Teira, Alfred P. Gillio, Hesham Eissa, Alan P. Knutsen, Frederick Goldman, Victor M. Aquino, Evan B. Shereck, Theodore B. Moore, Emi H. Caywood, Mark T. Vander Lugt, Jacob Rozmus, Larisa Broglie, Lolie C. Yu, Ami J. Shah, Jeffrey R. Andolina, Xuerong Liu, Roberta E. Parrott, Jasmeen Dara, Susan Prockop, Caridad A. Martinez, Malika Kapadia, Soma C. Jyonouchi, Kathleen E. Sullivan, Jack J. Bleesing, Sonali Chaudhury, Aleksandra Petrovic, Michael Keller, Troy C. Quigg, Suhag Parikh, Shalini Shenoy, Christine Seroogy, Tamar Rubin, Helene Decaluwe, John M. Routes, Troy R. Torgerson, Jennifer W. Leiding, Michael A. Pulsipher, Donald B. Kohn, Linda M. Griffith, Elie Haddad, Christopher C. Dvorak, Luigi D. Notarangelo
Summary: This study found that population-based newborn screening plays a crucial role in early detection and prompt treatment of SCID. The overall 5-year survival rate has significantly increased with the implementation of newborn screening, and the promotion of this screening method can benefit public health programs globally.
Article
Multidisciplinary Sciences
Xin Yong, Guowen Jia, Zhe Liu, Chunzhuang Zhou, Jiamin Yi, Li Chen, Lu Chen, Yuan Wang, Qingxiang Sun, Daniel D. Billadeau, Zhaoming Su, Da Jia
Summary: In this study, the structure of the Drosophila Mon1-Ccz1-RMC1 complex was determined using cryogenic-electron microscopy. RMC1 acts as a scaffolding subunit and binds to both Mon1 and Ccz1, explaining the binding specificity of the complex. The assembly of RMC1 with Mon1-Ccz1 is required for cellular RAB7A activation, autophagic functions, and organismal development in zebrafish.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Saki Nishikawa, Gaku Sato, Sakura Takada, Shunshi Kohyama, Gen Honda, Miho Yanagisawa, Yutaka Hori, Nobuhide Doi, Natsuhiko Yoshinaga, Kei Fujiwara
Summary: This study demonstrates that the co-existence of various macromolecules in cell-size space can regulate protein localization and biochemical reaction systems. Experimental and theoretical analyses show that a large surface-area-to-volume ratio enhances membrane localization of molecules, while competitive binding to lipid membranes among multiple proteins alleviates interfacial effects.
Review
Biochemistry & Molecular Biology
Kate L. Jordan, David J. Koss, Tiago F. Outeiro, Flaviano Giorgini
Summary: This review highlights the important roles of Rab GTPases in vesicle transport and membrane trafficking, and their implications in the pathogenesis of various diseases. It also provides an overview of the current state of pharmacological targeting of Rabs and discusses the challenges of therapeutically targeting these small proteins in humans, particularly in the context of Alzheimer's disease.
Article
Anesthesiology
Xinghua Ren, Siyi Zhang, Yongyan Yang, Annie Song, Feng Liang, Yiying Zhang, Yuanlin Dong, Xu Wu, Zhongcong Xie
Summary: It was found that ketamine can induce delirium-like behavior in mice and increase the amount of tau protein in the serum. Moreover, ketamine also leads to the accumulation of endosomes and inhibits endosome maturation, resulting in the accumulation of tau protein in endosomes and the cell culture medium. These findings suggest that ketamine may interfere with intracellular tau trafficking and induce delirium-like behavior.
ANESTHESIA AND ANALGESIA
(2023)