期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 14, 页码 5627-5632出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0901770106
关键词
bilayer lipid membrane; black lipid membrane; fluorescence interference contrast
资金
- National Institutes of Health [GM069630]
- National Science Foundation
- Stanford Bio-X Interdisciplinary Initiatives Program
- Benchmark Stanford Graduate fellowship
- Center for Probing the Nanoscale Graduate Prize
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0918782] Funding Source: National Science Foundation
Freestanding phospholipid bilayers have been assembled spanning shallow, micrometer-sized wells etched into a Si wafer substrate so that the bilayers are near (within hundreds of nanometers) but not in contact with the wafer surface. The proximity of the bilayers to the highly reflective Si/SiO2 interface allows them to be probed by using fluorescence-interference techniques. These interferometry measurements show that the bilayers are curved and that the curvature can be varied by changes in osmotic pressure. Furthermore, the ionophore gramicidin can be incorporated into the bilayers, making them selectively permeable to monovalent cations. This freestanding architecture may overcome surface-interaction problems that occur when cell membrane proteins are introduced into solid supported bilayers, while also allowing for high-precision measurements of changes in fluorophore position by interferometry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据