Article
Clinical Neurology
Jiaxi Yu, Xing-hua Luan, Meng Yu, Wei Zhang, He Lv, Li Cao, Lingchao Meng, Min Zhu, Binbin Zhou, Xiao-rong Wu, Pidong Li, Qiang Gang, Jing Liu, Xin Shi, Wei Liang, Zhirong Jia, Sheng Yao, Yun Yuan, Jianwen Deng, Daojun Hong, Zhaoxia Wang
Summary: This study identified and validated GGC repeat expansions in the NOTCH2NLC gene in three unrelated patients with distal weakness predominantly affecting distal lower limb muscles. Pathological findings in muscle and Schwann cells nuclei showed intranuclear inclusions with multiple proteins deposits. These results enrich the understanding of NOTCH2NLC-related repeat expansion disorders and suggest that the neurological symptoms might include not only nerve damage but also myopathy, with potential overlapping symptoms.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2021)
Article
Chemistry, Multidisciplinary
Le Yu, Peter Verwilst, Inseob Shim, Yu-Qiang Zhao, Ying Zhou, Jong Seung Kim
Summary: The nucleolus was identified as the main intracellular target of a new fluorescent probe, V-P1, which demonstrated selectivity to RNA G-quadruplexes and sensitivity to viscosity. The probe was able to accurately detect changes in intracellular viscosity under various conditions.
Article
Materials Science, Multidisciplinary
H. Lamsaf, V. Lenzi, L. Marques, L. Rebouta, S. Carvalho, L. F. Ballesteros, M. A. Cerqueira, J. A. Teixeira, L. Pastrana, S. Calderon
Summary: This study demonstrates a fast and simple method to obtain flower-like Zinc-Iron nanoparticles using a hybrid system combining conventional magnetron sputtering and a cluster beam source. Characterization of morphology and structure was done by STEM, while chemical composition was evaluated by EDS and EELS. Molecular dynamic simulations revealed the formation mechanisms of the flower-like nanoparticles during simultaneous production.
Review
Plant Sciences
Elizabeth Sarkel Smith, Zachary L. Nimchuk
Summary: Consistent flower production is crucial for robust agricultural yields in fluctuating environmental conditions. The plant hormone auxin plays a role in promoting flower development and interacts with molecular pathways that regulate stem cell maintenance. Recent advances suggest that auxin may integrate stem cell maintenance and new flower production.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Javad Esmaeili, Sorur Jadbabaee, Farnaz Mohebi Far, Mostafa Esmaeilpour Lukolayeh, Kevser Kubra Kirboga, Farnoush Sadat Rezaei, Aboulfazl Barati
Summary: Utilizing the stem of the Alstroemeria flower as a scaffold, this study fabricated a new scaffold material through decellularization and chitosan modification. The modified scaffold exhibited better porous structure, mechanical behavior, and biocompatibility compared to the unmodified scaffold. Modified tissue plants could be a promising candidate for tissue engineering of both soft and hard tissues.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biophysics
Shingo Tsukamoto, Takumi Asakawa, Shun Kimura, Naoyuki Takesue, Mohammad R. K. Mofrad, Naoya Sakamoto
Summary: In this study, local mechanical strains within the nucleus of a living cell subjected to substrate stretching were found to exhibit heterogeneous distribution, highlighting the importance of intranuclear mechanical properties heterogeneity. Treatment with Trichostatin A and Cytochalasin D did not cause significant changes in the intranuclear strain distribution, demonstrating the robustness of the observed phenomenon.
JOURNAL OF BIOMECHANICS
(2021)
Review
Clinical Neurology
Xiang Qin, Hao Chen, Chenguang Zhou, XiaoLong Wang, Jingjing Gao, Nan Guo, Yujing Wang, Shaodong Li
Summary: Neuronal intranuclear inclusion disease (NIID) is a rare neurodegenerative disease characterized by the presence of eosinophilic intranuclear inclusions in the nervous system and multiple visceral organs. Adult-onset NIID patients often present with symptoms like headache, dizziness, and limb weakness, with characteristic high intensity signal changes in brain imaging. Skin biopsy pathology combined with genetic testing can aid in accurate diagnosis.
NEUROLOGICAL SCIENCES
(2021)
Article
Astronomy & Astrophysics
Joshua Isaacson, William I. Jay, Alessandro Lovato, Pedro A. N. Machado, Noemi Rocco
Summary: We introduce a new lepton-nucleus event generator called ACHILLES, which separates the primary interaction from hadron propagation in the nucleus, allowing for modularity and interface with existing codes. The generator is validated against high-quality electron-carbon scattering data and shows promising agreement in both inclusive and exclusive distributions. The generator also proposes novel observables for further testing of lepton-nucleus scattering models. ACHILLES can be extended to generate neutrino-nucleus scattering events.
Article
Cell Biology
Mara Cucinotta, Alex Cavalleri, John William Chandler, Lucia Colombo
Summary: Establishment of species-specific floral organ body plan involves coordinated spatiotemporal processes, with auxin playing a key role in tissue-specific biosynthesis and transport. Auxin functions differently in various floral contexts, and comparative studies are needed to understand the conservation and evolutionary role of auxin pathways in flowering plants.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2021)
Article
Plant Sciences
Malik G. Al-Ajlouni, Yahia A. Othman, Tala S. A'saf, Jamal Y. Ayad
Summary: The aim of this study was to assess the effect of five plant growth regulators (PGRs) on the growth, anatomy, yield, and flower quality of lily. The results showed that the application of BA and GA3 at a concentration of 50 mg l-1 significantly increased leaf area and flower number per stem, as well as extended vase life. Additionally, Ancymidol at a concentration of 50 mg l-1 reduced stem elongation without adverse effects on leaf and flower quality. Overall, foliar application of PGRs at a concentration of 50 mg l-1 holds promise for improving flower quality of lily cultivars.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Lina Cheng, Ruizhen Li, Xiaoyang Wang, Siqi Ge, Sai Wang, Xianfeng Liu, Jing He, Cai-Zhong Jiang, Mingfang Qi, Tao Xu, Tianlai Li
Summary: The SlCLV3-SlWUS signaling pathway plays a significant role in low light-induced abscission in plants. Low light induces the upregulation of the SlCLV3 gene, which in turn inhibits the expression of the SlWUS gene, leading to disruptions in auxin response gradient and increased ethylene production, ultimately resulting in organ abscission.
Article
Clinical Neurology
Iulian V. Podar, Daniel A. P. Gutmann, Florian Harmuth, Tobias B. Haack, Stephan Ossowski, Holger Hengel, Antje Bornemann, Ludger Schoels, Oliver Neuhaus
Summary: This case report describes a Caucasian female patient who presented with paroxysmal encephalopathy twice. Diagnosis was made using various methods including neurocranium magnetic resonance imaging, skin biopsy for histology, and long-read genome sequencing. The patient's MRI findings were consistent with typical features of adult onset neuronal intranuclear inclusion disease. Skin biopsy confirmed the presence of intranuclear inclusion bodies, and long-read genome sequencing revealed an expansion of the GGC repeat in exon 1 of NOTCH2NLC. This case highlights the possibility of adult onset NOTCH2NLC-GGC-positive NIID in non-Asian individuals.
EUROPEAN JOURNAL OF NEUROLOGY
(2023)
Review
Cell Biology
Victoria N. Neckles, David M. Feliciano
Summary: Physiological functions require coordination among diverse organs, tissues, and cells. Microglia, immune cells in the brain, play a critical role in both defense against harmful pathogens and cellular debris as well as in neurophysiology.
CELL AND TISSUE RESEARCH
(2022)
Article
Food Science & Technology
Sara Ramirez-Bolanos, Jara Perez-Jimenez, Sara Diaz, Lidia Robaina
Summary: The study suggests that banana flowers and banana pseudo-stem have the potential to be used as novel food ingredients based on a preliminary analysis of phenolic compounds.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Plant Sciences
Marina M. Strelin, Pamela K. Diggle, Marcelo A. Aizen
Summary: Crop improvement should take into account the coordinated and predictable response of plants to genetic and environmental variation, particularly the impact of flower heterochrony on crop yield and hybrid crop varieties.
TRENDS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Omar Hamdani, Namrita Dhillon, Tsung-Han S. Hsieh, Takahiro Fujita, Josefina Ocampo, Jacob G. Kirkland, Josh Lawrimore, Tetsuya J. Kobayashi, Brandon Friedman, Derek Fulton, Kenneth Y. Wu, Rzvan Chereji, Masaya Oki, Kerry Bloom, David J. Clark, Oliver J. Rando, Rohinton T. Kamakaka
MOLECULAR AND CELLULAR BIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Colleen J. Lawrimore, Josh Lawrimore, Yunyan He, Sergio Chavez, Kerry Bloom
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
(2020)
Article
Multidisciplinary Sciences
E. Nelsen, C. M. Hobson, M. E. Kern, J. P. Hsiao, E. T. O'Brien, T. Watanabe, B. M. Condon, M. Boyce, S. Grinstein, K. M. Hahn, M. R. Falvo, R. Superfine
SCIENTIFIC REPORTS
(2020)
Article
Biophysics
Chad M. Hobson, E. Timothy O'Brien, Michael R. Falvo, Richard Superfine
BIOPHYSICAL JOURNAL
(2020)
Article
Cell Biology
Chad M. Hobson, Megan Kern, E. Timothy O'Brien, Andrew D. Stephens, Michael R. Falvo, Richard Superfine
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Biochemistry & Molecular Biology
Yunyan He, Josh Lawrimore, Diana Cook, Elizabeth Erin Van Gorder, Solenn Claire De Larimat, David Adalsteinsson, M. Gregory Forest, Kerry Bloom
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Josh Lawrimore, Daniel Kolbin, John Stanton, Muznah Khan, Solenn C. de Larminat, Colleen Lawrimore, Elaine Yeh, Kerry Bloom
Summary: The nucleolus is the site of ribosome biosynthesis, where the rDNA locus and Cdc14 protein behave like a condensate formed by polymer-polymer phase separation, while ribonucleoproteins behave like a condensate formed by liquid-liquid phase separation. The compaction and distribution in the nucleolus are dependent on the concentration of DNA cross-linkers.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Diana Cook, Sarah Long, John Stanton, Patrick Cusick, Colleen Lawrimore, Elaine Yeh, Sarah Grant, Kerry Bloom
Summary: The study revealed that the pericentromere region of chromosomes is a site of confined biochemical activities, and the repair of chromosome breaks varies depending on the distance and position between centromeres. Different repair pathways are utilized for breaks in the pericentromeric chromatin versus breaks in the arms of chromosomes, indicating spatial control over DNA repair pathways in different nuclear regions.
Article
Cell Biology
Josh Lawrimore, Kerry Bloom
Summary: The centromere is crucial for the accurate segregation of chromosomes during cell division. By studying the physical structure and proteins enriched in centromeric chromatin, we propose a mechanism for building tension between sister kinetochores, where the centromere is organized by loop-extruding proteins into a bottlebrush arrangement, enabling it to resist pulling forces during mitosis.
JOURNAL OF CELL SCIENCE
(2022)
Review
Genetics & Heredity
Kerry Bloom, Daniel Kolbin
Summary: The entire genome undergoes changes following DNA damage, and understanding the mechanisms at the genome level requires both experimental and computational approaches to study the behavior of long-chain DNA polymers. Principles from polymer physics play a crucial role in unraveling the mysteries hidden in the chains of life.
Review
Engineering, Biomedical
Chad M. Hobson, Michael R. Falvo, Richard Superfine
Summary: The cell nucleus is not just a location for DNA, but also a complex material that resists physical deformations and dynamically responds to external mechanical cues. The molecules responsible for nuclear mechanical properties can contribute to laminopathies and potentially play a role in cellular mechanotransduction and physical processes in cancer. Studying nuclear mechanics and the downstream biochemical consequences or their modulation requires a suite of complex assays for applying, measuring, and visualizing mechanical forces across diverse length, time, and force scales.
APL BIOENGINEERING
(2021)
Article
Cell Biology
Prashant K. Mishra, Wei-Chun Au, Pedro G. Castineira, Nazrin Ali, John Stanton, Lars Boeckmann, Yoshimitsu Takahashi, Michael Costanzo, Charles Boone, Kerry S. Bloom, Peter H. Thorpe, Munira A. Basrai
Summary: This study reveals the role of cell cycle-regulated methylation of Cse4-R37 in high-fidelity chromosome segregation and highlights the importance of epigenetic modifications, such as methylation of kinetochore proteins, in preventing CIN, an important hallmark of human cancers.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Meeting Abstract
Pediatrics
P. Sears, H. Gong, M. R. Markovetz, X. Bustamante-Marin, R. Superfine, D. B. Hill, L. Ostrowski
PEDIATRIC PULMONOLOGY
(2020)
Meeting Abstract
Biophysics
Chad Hobson, Evan F. Nelsen, Joe Hsiao, Megan E. Kern, Andrew Stephens, E. Timothy O'Brien, Michael R. Falvo, Richard Superfine
BIOPHYSICAL JOURNAL
(2020)
Meeting Abstract
Biophysics
Megan E. Kern, Evan F. Nelsen, Chad M. Hobson, Joe Hsiao, E. Timothy E. O'Brien, Michael R. Falvo, Richard Superfine
BIOPHYSICAL JOURNAL
(2020)