Article
Multidisciplinary Sciences
Kento Ojima, Kazuki Shiraiwa, Kyohei Soga, Tomohiro Doura, Mikiko Takato, Kazuhiro Komatsu, Michisuke Yuzaki, Itaru Hamachi, Shigeki Kiyonaka
Summary: The method presented in the study combines affinity-based receptor labeling and bioorthogonal click chemistry to quantify AMPAR distribution and trafficking under physiological conditions. This versatile tool is successfully expanded to selectively label NMDA-type glutamate receptors.
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Monique Lillis, Nathan J. Zaccardi, Maxwell G. Heiman
Summary: In this study, it was found that Caenorhabditis elegans amphid neurons exhibit both neuronal and epithelial features, with a distal portion of the dendrite being apical and the rest of the dendrite, soma, and axon being basolateral. The localization of the conserved adhesion molecule SAX-7/L1CAM was studied, and it was found that the 91-aa cytoplasmic tail of SAX-7 is necessary and sufficient for basolateral localization. Short tail sequences resembling known sorting motifs in mammalian epithelia were also found to be able to direct basolateral localization. Changing just two residues in a short motif was sufficient to redirect the protein between apical, basolateral, and axonal localization.
Review
Neurosciences
Jeremy M. Henley, Jithin D. Nair, Richard Seager, Busra P. Yucel, Gavin Woodhall, Benjamin S. Henley, Karolina Talandyte, Hope I. Needs, Kevin A. Wilkinson
Summary: Epilepsy is caused by abnormal neuronal network activity. The review focuses on the role and regulation of kainate and AMPA receptors in seizure activity, discussing the impact of GluK2 and GluA2 subunits on their respective receptors' properties.
Article
Neurosciences
Anna Bodzeta, Nicky Scheefhals, Harold D. MacGillavry
Summary: The various functions of glutamate in the brain are mediated by ionotropic and metabotropic glutamate receptors, which modulate synaptic transmission and plasticity. mGluRs play roles at both presynaptic and postsynaptic sites, acting as essential signal integrators that couple mechanisms of transmission and plasticity.
Review
Behavioral Sciences
Wolfgang Loescher, Eugen Trinka
Summary: There is strong clinical evidence supporting the potential use of topiramate (TPM) for refractory and super-refractory status epilepticus. However, as TPM is currently only approved for oral administration, the development of an intravenous solution is highly desirable for emergency situations.
EPILEPSY & BEHAVIOR
(2023)
Article
Biochemistry & Molecular Biology
Na Wang, Dan-Dan Wang, Xiaolin Hou, Xiumao Li, Ying Shen
Summary: Numb protein isoforms Numb-p72 and Numb-p65 exhibit distinct expression patterns in HEK293T cells, with Numb-p72 specifically binding to mGluR5 alpha and enhancing its membrane expression by inhibiting internalization. This indicates that a complete structure of Numb is necessary for binding to and modulating mGluR5 trafficking.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Cell Biology
Lisette Sandoval, Mariana Labarca, Claudio Retamal, Paula Sanchez, Juan Larrain, Alfonso Gonzalez
Summary: This study reveals the mechanism of how newly synthesized Hedgehog proteins are transported within polarized epithelial cells, from the trans-Golgi network to the cell surface, and then to different domains. This process is regulated by the lipid modifications and mediated by the protein Dispatched-1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Pankajam Thyagarajan, Chengye Feng, David Lee, Matthew Shorey, Melissa M. Rolls
Summary: Microtubule polarity plays a crucial role in neuronal polarization by influencing other cytoskeletal features, although changes in microtubule polarity have minimal effects on overall neuron shape.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Rabia R. Khawaja, Amit Agarwal, Masahiro Fukaya, Hey-Kyeong Jeong, Scott Gross, Estibaliz Gonzalez-Fernandez, Jonathan Soboloff, Dwight E. Bergles, Shin H. Kang
Summary: Regulation of AMPAR GluA2 content in oligodendrocyte precursor cells affects cell proliferation and regenerative behavior, serving as a potential target for myelin repair.
Article
Biochemistry & Molecular Biology
Jean-Baptiste Brault, Sabine Bardin, Marusa Lampic, Jacopo A. Carpentieri, Laure Coquand, Maxime Penisson, Hugo Lachuer, Guiliana Soraya Victoria, Sarah Baloul, Fatima El Marjou, Gaelle Boncompain, Stephanie Miserey-Lenkei, Richard Belvindrah, Vincent Fraisier, Fiona Francis, Franck Perez, Bruno Goud, Alexandre D. Baffet
Summary: This study reveals that the delamination process of radial glial (RG) cells in the developing neocortex is regulated by the post-Golgi secretory pathway. The transport of RAB6+ vesicles towards the minus ends of microtubules depends on dynein, and the apical determinant Crumbs3 (CRB3) is also transported by dynein. Knockout of RAB6A/A' and RAB6B, as well as LIS1, impairs the apical localization of CRB3 and leads to delamination and ectopic division of aRG cells.
Article
Engineering, Environmental
Zabish Bilew Muche, Yosef Nikodimos, Teshager Mekonnen Tekaligne, Semaw Kebede Merso, Tripti Agnihotri, Gashahun Gobena Serbessa, She-Huang Wu, Wei-Nien Su, Bing Joe Hwang
Summary: A novel 3D cross-linked and thermotolerant fluorinated polyimide/polyvinylidene fluoride-hexafluoropropylene hybrid nanofiber separator with high porosity, excellent thermal stability, and flexibility was developed. The separator exhibited outstanding electrolyte uptake ability, strong mechanical properties, and achieved stable Li plating/stripping. When applied in anode-free Li-metal batteries or Li-ion batteries, the cells showed high capacity and long lifetime. The hybrid separator also demonstrated robust functionality even after being heated at a high temperature.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biology
Marina Goncalves Antunes, Matthieu Sanial, Vincent Contremoulins, Sandra Carvalho, Anne Plessis, Isabelle Becam
Summary: This study reveals how the hedgehog (HH) morphogen regulates the subcellular localization and activity of the oncogenic G-protein-coupled receptor (GPCR) Smoothened (SMO), thereby affecting the morphogenesis of epithelial structures. The authors provide evidence that HH promotes the recycling of internalized SMO, leading to its stabilization. This effect relies on the action of protein kinase A, casein kinase I, and Fused (FU) kinase. Additionally, at high HH levels, FU leads to the local enrichment of SMO in the basal domain of the cell membrane.
Review
Plant Sciences
Huibin Han, Maciek Adamowski, Linlin Qi, Saqer S. Alotaibi, Jiri Friml
Summary: Plants exhibit differential growth responses through the coordination of auxin hormone and PIN auxin exporters during tropisms, which are regulated by environmental stimuli. Auxin gradients promote cell elongation and bending, with auxin feedback playing a crucial role in PIN polarity during bending termination. Interactions between different tropisms are reviewed to enhance understanding of plant adaptive growth in the natural environment.
Article
Multidisciplinary Sciences
Austin M. Ramsey, Ai-Hui Tang, Tara A. LeGates, Xu-Zhuo Gou, Beatrice E. Carbone, Scott M. Thompson, Thomas Biederer, Thomas A. Blanpied
Summary: Recent evidence suggests that nano-organization of proteins within synapses may control the strength of communication between neurons in the brain. Targeting LRRTM2, a key transsynaptic adhesion molecule, and disrupting its extracellular domain quickly led to nanoscale declustering of AMPARs, resulting in significant deficits in evoked postsynaptic receptor activation but not spontaneous activation. This indicates that adhesion molecules may acutely position receptors to dynamically control synaptic strength.
Article
Genetics & Heredity
Jia-Hui Sun, Jiang Chen, Fernando Eduardo Ayala Valenzuela, Carolyn Brown, Diane Masser-Frye, Marilyn Jones, Leslie Patron Romero, Berardo Rinaldi, Wenhui Laura Li, Qing-Qing Li, Dan Wu, Benedicte Gerard, Erin Thorpe, Allan Bayat, Yun Stone Shi
Summary: A pathogenic missense variant in the GRIA3 gene was identified in a female patient with severe epilepsy and global developmental delay. This variant enhances glutamate signaling by strengthening the activity of glutamate receptors, leading to epileptic encephalopathy and global developmental delay.
Article
Ophthalmology
Milica A. Margeta, Edward G. Buckley, Mays A. El-Dairi
Article
Ophthalmology
Milica A. Margeta, Anthony N. Kuo, Alan D. Proia, Sharon F. Freedman
Review
Neurosciences
Milica A. Margeta, Kang Shen, Brock Grill
CURRENT OPINION IN NEUROBIOLOGY
(2008)
Article
Ophthalmology
Milica A. Margeta, Eleonora M. Lad, Alan D. Proia
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2018)
Article
Neurosciences
Milica A. Margeta
JOURNAL OF NEUROSCIENCE
(2008)
Article
Neurosciences
Milica A. Margeta, Kang Shen
MOLECULAR AND CELLULAR NEUROSCIENCE
(2010)
Editorial Material
Ophthalmology
Billy X. Pan, Milica A. Margeta
JAMA OPHTHALMOLOGY
(2019)
Article
Ophthalmology
Milica A. Margeta, Kitiya Ratanawongphaibul, Edem Tsikata, Michele Zemplenyi, Courtney L. Ondeck, Janice Kim, Anne L. Coleman, Fei Yu, Johannes F. de Boer, Teresa C. Chen
Summary: The presence of optic disc hemorrhages in open-angle glaucoma patients was associated with localized neuroretinal rim thickness progression, but not with other progression parameters. Glaucoma progression detected through high-density 3D SD-OCT measurements often preceded the occurrence of DH, indicating that DHs are indicators of ongoing glaucoma progression rather than discrete events causing subsequent progression.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Immunology
Milica A. Margeta, Zhuoran Yin, Charlotte Madore, Kristen M. Pitts, Sophia M. Letcher, Jing Tang, Shuhong Jiang, Christian D. Gauthier, Sebastian R. Silveira, Caitlin M. Schroeder, Eleonora M. Lad, Alan D. Proia, Rudolph E. Tanzi, David M. Holtzman, Susanne Krasemann, Dong Feng Chen, Oleg Butovsky
Summary: This study shows that the APOE4 allele has a neuroprotective effect in glaucoma by impairing the activation of microglia. Additionally, targeting the APOE4-Galectin-3 signaling pathway may be a potential therapeutic approach for glaucoma.
Meeting Abstract
Ophthalmology
Yapei Zhang, Kitiya Ratanawongphaibul, Edem Tsikata, Michele Zemplenyi, Hang Lee, Milica A. Margeta, Courtney L. Ondeck, Anne L. Coleman, Fei Yu, Johannes F. de Boer, Teresa C. Chen
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Daniel A. Colon-Ramos, Milica A. Margeta, Kang Shen