期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 34, 页码 12118-12122出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0805286105
关键词
E. coli; scanning electrochemical microscope; silver ion
资金
- NUCRYST Pharmaceuticals
We report that silver ion (Ag+) uptake is enhanced by 4-aminopyridine (4-AP), a well known voltage-sensitive potassium ion channel (K.) blocker. Both bacterial (Escherichia coli) and mammalian (3T3 fibroblast) cells were used as model systems. Ag+ uptake was monitored with a scanning electrochemical microscope with an amperometric Ag+ ion-selective electrode (Ag+-ISE) and the respiration rates of E. coli cells were measured by oxygen reduction at an ultramicroelectrode. The results showed that not only the amount but also the rate of silver uptake by the cells increased significantly when 4-AP was added to the solution. For fibroblasts, the Ag+ uptake rate was 4.8 x 10(7) ions per cell per sec without 4-AP compared with 1.0 x 10(8) ions per cell per sec with 0.2 mM 4-AP. For E. coli cells, the uptake rate was 1.5 x 10(4) ions per cell per sec without 4-AP vs. 3.5 x 10(4) ions per cell per sec with 0.5 mM 4-AP and 5.9 x 10(4) ions per cell per sec with 1 mM 4-AP. Thus, 4-AP might be useful where silver is used as antimicrobial agent to speed its uptake.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据