期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 29, 页码 10011-10016出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0800031105
关键词
intracellular transport; molecular motors; kinesin; dynein; cytoskeleton
资金
- NIAMS NIH HHS [R01 AR044420, R29 AR044420, AR44420] Funding Source: Medline
- NIGMS NIH HHS [GM-52111, R01 GM052111] Funding Source: Medline
We study the role of microtubule movement in bidirectional organelle transport in Drosophila S2 cells and show that EGFP-tagged peroxisomes in cells serve as sensitive probes of motor induced, noisy cytoskeletal motions. Multiple peroxisomes move in unison over large time windows and show correlations with microtubule tip positions, indicating rapid microtubule fluctuations in the longitudinal direction. We report the first high-resolution measurement of longitudinal microtubule fluctuations performed by tracing such pairs of co-moving peroxisomes. The resulting picture shows that motor-dependent longitudinal microtubule oscillations contribute significantly to cargo movement along microtubules. Thus, contrary to the conventional view, organelle transport cannot be described solely in terms of cargo movement along stationary microtubule tracks, but instead includes a strong contribution from the movement of the tracks.
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