期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 39, 页码 15130-15135出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0806938105
关键词
fear conditioning; release probability; synaptic plasticity; synaptic transmission
资金
- Agence Nationale pour la Recherche
- European Neuroscience Institutes Network
- Eucor Learning and Teaching Mobility Program
- Swiss National Science Foundation
- Novartis Research Foundation
NMDA receptor-dependent long-term potentiation (LTP) of glutamatergic synaptic transmission in sensory pathways from auditory thalamus or cortex to the lateral amygdala (LA) underlies the acquisition of auditory fear conditioning. Whereas the mechanisms of postsynaptic LTP at thalamo-LA synapses are well understood, much less is known about the sequence of events mediating presynaptic NMDA receptor-dependent LTP at cortico-LA synapses. Here, we show that presynaptic cortico-LA LTP can be entirely accounted for by a persistent increase in the vesicular release probability. At the molecular level, we found that signaling via the cAMP/PKA pathway is necessary and sufficient for LTP induction. Moreover, by using mice lacking the active-zone protein and PKA target RIM1 alpha(RIM1 alpha(-/-)), we demonstrate that RIM1 alpha is required for both chemically and synaptically induced presynaptic LTP. Further analysis of cortico-LA synaptic transmission in RIM1 alpha(-/-) mice revealed a deficit in Ca2+-release coupling leading to a lower baseline release probability. Our results reveal the molecular mechanisms underlying the induction of presynaptic LTP at cortico-LA synapses and indicate that RIM1 alpha-dependent LTP may involve changes in Ca2+-release coupling.
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