Article
Multidisciplinary Sciences
Stefano Gnan, Joseph M. Josephides, Xia Wu, Manuela Spagnuolo, Dalila Saulebekova, Mylene Bohec, Marie Dumont, Laura G. Baudrin, Daniele Fachinetti, Sylvain Baulande, Chun-Long Chen
Summary: The researchers developed Kronos scRT, a software for analyzing single-cell Replication Timing (scRT). By applying this tool to large datasets, they found that aneuploidy has a minor impact on replication patterns, but different cell types and sub-populations exhibit distinct replication paths.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Lina-Marie Briu, Chrystelle Maric, Jean-Charles Cadoret
Summary: The replication-timing program is crucial in eukaryotic nuclear processes and is closely linked to transcriptional activity, epigenetic landscape, and the 3D organization of the genome. There is a complex relationship between replication timing, replication stress, and genomic instability, although the extent of their mutual connection requires further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Daniel J. Goodall, Katie H. Jameson, Michelle Hawkins, Christian J. Rudolph
Summary: The termination phase of DNA replication is complex, and in Escherichia coli, it involves the formation of a replication fork trap system facilitated by Tus protein binding to ter sites. While this system is present in some bacterial species, it is not a universal feature of bacterial chromosomes. The highly conserved ter sites in E. coli genomes, with slightly more variability in Shigella genomes, suggest the physiological significance of the replication fork trap system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Logan Richards, Souradip Das, Jared T. Nordman
Summary: Successful and accurate replication of the genome is crucial for maintaining genomic stability and preventing diseases. Replication timing (RT), which determines when each segment of the genome is replicated, is an important aspect of genome duplication. The protein Rif1 plays a key role in regulating RT, by delaying the activation of helicase in certain regions of the genome. However, many questions still remain regarding the mechanisms of Rif1 function.
Article
Biochemistry & Molecular Biology
Lilas Courtot, Elodie Bournique, Chrystelle Maric, Laure Guitton-Sert, Miguel Madrid-Mencia, Vera Pancaldi, Jean-Charles Cadoret, Jean-Sebastien Hoffmann, Valerie Bergoglio
Summary: This study reveals that low replication stress can lead to advanced DNA replication timing, which is cell-type specific and involves large heterochromatin domains. These advanced events can be inherited by the next generation of cells, leading to changes in chromatin accessibility, replication origin landscape, and gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Alexa N. Bracci, Anissa Dallmann, Qiliang Ding, Melissa J. Hubisz, Madison Caballero, Amnon Koren
Summary: Replication timing of DNA is linked to gene regulation and genome stability. The differences in replication timing between humans and chimpanzees suggest ongoing evolution of the DNA replication timing program. These differences are driven by DNA sequence alterations and can impact gene expression and chromatin structure.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Genetics & Heredity
Lilian Lanteri, Anthony Perrot, Diane Schausi-Tiffoche, Pei-Yun Jenny Wu
Summary: Eukaryotic DNA replication is regulated by conserved mechanisms, and changes in the position of replication origins along the chromosome can lead to alterations in the organization of DNA replication. However, large-scale genomic inversions surprisingly do not affect cell proliferation and meiotic progression, despite inducing restructuring of replication domains. The immediate chromosomal neighborhood of an origin is a crucial determinant of its activity.
Review
Biochemistry & Molecular Biology
Janusz Blasiak, Joanna Szczepanska, Anna Sobczuk, Michal Fila, Elzbieta Pawlowska
Summary: RIF1 is a master regulator of DNA replication timing in human cells, interacting with TP53BP1 to play crucial roles in fork reactivation and DNA double-strand break repair. Its functions are associated with maintaining 3D chromatin structure and epigenetic markers disrupted by DNA double-strand breaks, highlighting its significance in genomic stability and potential applications in cancer diagnostics, prognosis, and therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, Research & Experimental
Isaac Kyei Barffour, Desmond Omane Acheampong
Summary: Replication licensing is crucial for eukaryotic chromosomal DNA replication, failure of which can lead to genome instability syndromes. Understanding the mechanisms of replication licensing can help in identifying targets for treating genome instability disorders.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Jiho Park, Song Y. Yeu, Sangjin Paik, Hyungmin Kim, Si-Young Choi, Junyeop Lee, Jinho Jang, Semin Lee, Youngil Koh, Hyunsook Lee
Summary: Loss of BubR1 acetylation leads to chromosomal rearrangement and genetic instability in mice, with replication stress playing a key role in these processes. Moreover, defects in BubR1 acetylation in mitosis contribute to tumorigenesis, as observed in human cancer cells with whole-arm translocations demonstrating similar defects.
Article
Biochemical Research Methods
Amnon Koren, Dashiell J. Massey, Alexa N. Bracci, Peter Robinson
Summary: TIGER is a computational approach for extracting DNA replication timing information from whole genome sequence data, applicable to any species with a contiguous genome assembly. Replication dynamics can be observed by analyzing DNA copy number along chromosomes, providing a straightforward approach for measuring replication timing.
Article
Multidisciplinary Sciences
Magdalena Jedrzejczak-Silicka, Adam Lepczynski, Filip Golebiowski, Daniel Dolata, Andrzej Dybus
Summary: The study analyzed a microsatellite polymorphism in the pigeon's LDHA gene using HRM method, classifying birds into five genotypes based on the number of repeat units. HRM was confirmed to be effective for rapid identification of the microsatellite polymorphism.
Article
Genetics & Heredity
Livio Dukaj, Nicholas Rhind
Summary: Research suggests that the loading of MCM replicative helicase at replication origins is a key determinant of replication timing. The study found that some origins are more sensitive to reductions in MCM levels, leading to delays in replication. Additionally, the loading capacity of origins is considered the primary determinant of MCM stoichiometry.
Article
Biochemistry & Molecular Biology
Yue Wang, Yunchao Huang, Edith Cheng, Xinhua Liu, Yu Zhang, Jianguo Yang, Jordan T. F. Young, Grant W. Brown, Xiaohan Yang, Yongfeng Shang
Summary: This study reveals an unexpected role for lysine-specific demethylase 1 (LSD1) in euchromatic origin firing and replication timing, highlighting the importance of epigenetic regulation in the activation of replication origins. The findings support the potential use of selective inhibitors of LSD1 in cancer therapy, while emphasizing the need to maintain an appropriate level of LSD1 to minimize side effects.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Cell Biology
Athanasios E. Vouzas, David M. Gilbert
Summary: Decades of research on the spatiotemporal organization of mammalian DNA replication timing has revealed correlations with transcription and chromatin organization. Recent studies have shown that the replication timing program is influenced by and necessary for maintaining chromatin structure, forming an epigenetic feedback loop. Specific cis-acting elements have been discovered to regulate mammalian replication timing, revealing diverse mechanisms of control in different cell types.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2023)