Article
Engineering, Biomedical
Ross Burdis, Xavier Barcelo Gallostra, Daniel J. Kelly
Summary: Scaffold-free tissue engineering can generate more hyaline-like cartilage tissue compared to single-cell scaffold-free approaches. Temporal enzymatic treatment can enhance microtissue fusion and tissue remodeling, leading to the formation of a denser and more biomimetic zonal collagen network. This study demonstrates that using biological building blocks can be improved by using temporal enzymatic treatment during graft development.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Multidisciplinary Sciences
Anne K. Haudenschild, Benjamin E. Sherlock, Xiangnan Zhou, Clay S. Sheaff, Jerry C. Hu, J. Kent Leach, Laura Marcu, Kyriacos A. Athanasiou
Summary: This article introduces a non-destructive, inexpensive, and rapid sampling and analysis system that can continuously monitor the mechanical, biochemical, and structural properties of tissue samples. By combining fluorescent lifetime imaging and ultrasound backscatter microscopy through a fiber-based interface, it enables sterile monitoring of tissue quality.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Amin Joukar, Amy Creecy, Sonali Karnik, Hessam Noori-Dokht, Stephen B. Trippel, Joseph M. Wallace, Diane R. Wagner
Summary: The main objective of this study was to assess the relationship between the wear resistance of healthy articular cartilage and its biochemical composition and viscoelastic material properties. The findings suggest that stiffer cartilage with higher glycosaminoglycans, collagen, and water content has a higher wear resistance. Enzymatic collagen crosslinks also enhance the wear resistance of the collagen network.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Neurosciences
Julian Guarque-Chabrera, Aitor Sanchez-Hernandez, Patricia Ibanez-Marin, Ignasi Melchor-Eixea, Marta Miquel
Summary: This study investigates the role of perineuronal nets (PNNs) in cocaine-induced conditioned preference. The results show that digestion of PNNs disrupts short-term memory of conditioned preference but facilitates extinction formation. Moreover, PNN degradation also encourages the reinstatement of cocaine-induced conditioned preference. Therefore, degradation of PNNs may be a promising tool to manipulate drug-induced memory.
Article
Orthopedics
Elias Salzer, Vivian H. M. Mouser, Marianna A. Tryfonidou, Keita Ito
Summary: This study aimed to investigate biomechanical restoration through the development of an ex vivo NP explant model with reduced sGAG content and injection of proteoglycan-containing NCM. Results showed that ChABC injection induced dose-dependent sGAG reduction, diurnal loading helped reduce sGAG loss, and NCM, while not providing instant biomechanical or biochemical restoration, showed potential for biological repair. Future studies should focus on biomaterial/NCM combinations to better retain NCM within NP tissue.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Orthopedics
Jarrett M. Link, Jerry C. Hu, Kyriacos A. Athanasiou
Summary: The study aimed to enhance the in vitro integration of self-assembled articular cartilage to native articular cartilage using chondroitinase ABC. It was found that the effective C-ABC(int) dose depends on the maturity level of the neocartilage, while the mechanical properties of the constructs were not affected by the dose of C-ABC(int).
Review
Engineering, Biomedical
Vishal Thomas, Jeremy Mercuri
Summary: This paper provides an in-depth investigation of the performance of naturally derived materials used in repairing/regenerating articular cartilage. While most natural constructs with native biomolecules have shown sub-optimal results in the past, current trends suggest that these materials have promising potential for effective scaffold fabrication.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Weijiao Zhang, Ruirui Xu, Xuerong Jin, Yang Wang, Litao Hu, Tianmeng Zhang, Guocheng Du, Zhen Kang
Summary: In this study, enzymatic strategies for producing homogeneous chondroitin oligosaccharides (o-CHs) and its sulfate derivatives from microbial sourced chondroitin were reported. The enzymatic approaches showed high conversion rates and pave the way for the study of structure-activity relationship and applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Biomaterials
Xavier Barcelo, Orquidea Garcia, Daniel J. Kelly
Summary: The meniscus is a crucial tissue in the knee joint, but its limited regenerative capacity has driven the development of tissue engineering approaches. In this study, melt electrowriting was used to create structured scaffolds, and enzymatic treatment was employed to enhance collagen network maturity. This resulted in the development of engineered meniscus tissues with improved mechanical properties.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Tianyu Gao, Alexander J. Boys, Crystal Zhao, Kiara Chan, Lara A. Estroff, Lawrence J. Bonassar
Summary: This study utilized confocal Raman microspectroscopy to investigate the degradation of articular cartilage, specifically focusing on spatially mapping the loss of glycosaminoglycans (GAGs), providing a technique with high spatial resolution to measure cartilage degradation without tissue sections.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Ryan Borem, Joshua Walters, Allison Madeline, Lee Madeline, Sanjitpal Gill, Jeremiah Easley, Jeremy Mercuri
Summary: A comprehensive characterization of a sheep model of ChABC-induced IVDD demonstrated significant structural and functional changes, suggesting its potential use in evaluating biomaterials for IVD repair, regeneration, or fusion.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Biochemistry & Molecular Biology
Brynna S. Eisele, Zigmund Luka, Alice J. Wu, Fei Yang, Andrew T. Hale, John D. York
Summary: BPNT2 is a key enzyme involved in GAG sulfation, crucial for proper extracellular matrix development. Inhibition or mutations of BPNT2 can lead to impaired sulfation and chondrodysplasia, with potential implications for lithium pharmacology.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Orthopedics
Benjamin Andress, Maureane Hoffman, Amy L. McNulty
Summary: Orthopaedic research, like biomedical research in general, has made significant progress in developing treatments for previously untreatable conditions. However, there is a concern about the quality of published research. This commentary highlights the importance of analytical validation and quality control in orthopaedic research and proposes recommendations for implementing these practices to improve research rigor and reproducibility.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Medicine, General & Internal
Rodolfo de Melo Nunes, Virginia Claudia Carneiro Girao, Pablyana Leila Rodrigues Cunha, Judith Pessoa Andrade Feitosa, Ana Carolina Matias Dinelly Pinto, Francisco Airton Castro Rocha
Summary: The study aimed to determine the characteristics of glycosaminoglycans (GAGs) in osteoarthritis and normal cartilage, as well as in men and women. Results showed that GAG content was higher in women compared to men, and in osteoarthritis samples compared to fracture samples, with a reduction in sulfur content in GAG from osteoarthritis patients.
FRONTIERS IN MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Parkash Singh Rawat, Yan Li, Weixin Zhang, Xiangfeng Meng, Weifeng Liu
Summary: An increased understanding of GAG metabolism by intestinal bacteria is critical in identifying the driving factors for the composition, modulation, and homeostasis of the human gut microbiota. In this study, a highly efficient GAG-degrading Firmicutes bacterium and the first chondroitin ABC lyase from a Firmicutes were isolated and characterized, providing new insights into GAG utilization in the human colon. The findings suggest that Firmicutes may have a distinct approach to catabolize GAGs compared to Bacteroides. The characterized chondroitin ABC lyase has a high specific activity, which aids in the development of a commercial chondroitinase for industrial and medicinal applications.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Engineering, Biomedical
Jennifer H. Edwards, Anthony Herbert, Gemma L. Jones, Iain W. Manfield, John Fisher, Eileen Ingham
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2017)
Article
Cell & Tissue Engineering
Tayyebeh Vafaee, Daniel Thomas, Amisha Desai, Louise M. Jennings, Helen Berry, Paul Rooney, John Kearney, John Fisher, Eileen Ingham
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2018)
Article
Biophysics
P. Bowland, E. Ingham, J. Fisher, L. M. Jennings
JOURNAL OF BIOMECHANICS
(2018)
Article
Biochemistry & Molecular Biology
A. Barco, E. Ingham, J. Fisher, H. Fermor, R. P. W. Davies
JOURNAL OF PEPTIDE SCIENCE
(2018)
Article
Engineering, Biomedical
A. Desai, T. Vafaee, P. Rooney, J. N. Kearney, H. E. Berry, E. Ingham, J. Fisher, L. M. Jennings
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2018)
Article
Engineering, Biomedical
Philippa Bowland, Eileen Ingham, John Fisher, Louise M. Jennings
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART H-JOURNAL OF ENGINEERING IN MEDICINE
(2018)
Article
Engineering, Biomedical
Jennifer Helen Edwards, Eileen Ingham, Anthony Herbert
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2019)
Article
Multidisciplinary Sciences
Aiqin Liu, Eileen Ingham, John Fisher, Louise M. Jennings
Article
Cell & Tissue Engineering
S. Whitaker, J. H. Edwards, S. Guy, E. Ingham, A. Herbert
BONE & JOINT RESEARCH
(2019)
Article
Engineering, Biomedical
Philippa Bowland, Raelene M. Cowie, Eileen Ingham, John Fisher, Louise M. Jennings
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART H-JOURNAL OF ENGINEERING IN MEDICINE
(2020)
Article
Multidisciplinary Sciences
Aiqin Liu, William J. Sanderson, Eileen Ingham, John Fisher, Louise M. Jennings
Article
Cell & Tissue Engineering
Halina T. Norbertczak, Eileen Ingham, Hazel L. Fermor, Ruth K. Wilcox
TISSUE ENGINEERING PART C-METHODS
(2020)
Article
Biotechnology & Applied Microbiology
Reem El-Gendy, Sarah Junaid, Stephen K. L. Lam, Karen M. Elson, Joanne L. Tipper, Richard M. Hall, Eileen Ingham, Jennifer Kirkham
Summary: This study established a porcine tooth in situ organ culture system successfully maintained over 4 days in vitro. The viability, maintenance of structure, and sterility of the model were confirmed through various assays and analysis methods, demonstrating its potential for future research in periodontal regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Ashley Ward, Debora Morgante, John Fisher, Eileen Ingham, Jennifer Southgate
Summary: Bladder acellular matrix has potential applications in reconstructive surgeries, but the decellularization process is complex. This study used a mechanical engineering approach to design a laser-printed flat-bed apparatus for reproducible and full-thickness bladder tissue decellularization.
BIOMEDICAL MATERIALS
(2021)
Article
Cell Biology
A. Aldridge, A. Desai, H. Owston, L. M. Jennings, J. Fisher, P. Rooney, J. N. Kearney, E. Ingham, S. P. Wilshaw
CELL AND TISSUE BANKING
(2018)