Review
Microbiology
Karolina Plaskowska, Jolanta Zakrzewska-Czerwinska
Summary: Bdellovibrio bacteriovorus, a predatory bacterium, has a unique lifecycle and proliferation mode that are of great interest for studying bacterial cell biology. Its chromosome undergoes remodeling and replication initiation in a distinct manner.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Microbiology
Kathrin Alpers, Elisabeth Vatareck, Lothar Groebe, Mathias Muesken, Maren Scharfe, Susanne Haeussler, Jurgen Tomasch
Summary: Pseudomonas aeruginosa undergoes a transition from fast growth with overlapping cell cycles to sustained slow growth with non-overlapping cell cycles when cultivated under standard laboratory conditions. This transition is characterized by sequential changes in transcriptional activity along the chromosome and a shift in quorum sensing activity. RNA sequencing of subpopulations identified distinct gene expression patterns for replicating and nonreplicating cells.
Article
Biology
Kanika Khanna, Javier Lopez-Garrido, Joseph Sugie, Kit Pogliano, Elizabeth Villa
Summary: Bacillus subtilis can divide via two modes, forming different septa. During sporulation, the division septum is closer to one pole, resulting in unequal-sized forespores and mother cells. FtsAZ filaments have different distribution in the two septa, impacting septal thickness.
Article
Biochemistry & Molecular Biology
Nicolas Luis Calzetta, Marina Alejandra Gonzalez Besteiro, Vanesa Gottifredi
Summary: This study found that PARP inhibition selectively alleviates reduced fork elongation rates in Chk1-depleted cells, but does not relieve fork asymmetry. PARPs not only affect fork movement, but also induce fork deceleration and increase DNA synthesis rates in Chk1-depleted cells.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Tingting Zou, Zhenghong Lin
Summary: The paragraph explains how the cell cycle is regulated by the division of cellular components and the activation of cyclin-dependent kinases. The ubiquitin-proteasome system plays a crucial role in regulating cell proliferation, genomic stability, and cancer occurrence through recognition and modification of key proteins in the cell cycle machinery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Yichen Sun, Yan Gao, Jianfeng Chen, Ling Huang, Peng Deng, Jinghong Chen, Kelila Xin Ye Chai, Jing Han Hong, Jason Yongsheng Chan, Haixia He, Yali Wang, Daryl Cheah, Jing Quan Lim, Burton Kuan Hui Chia, Dachuan Huang, Lizhen Liu, Shini Liu, Xiaoxiao Wang, Yan Teng, Diwen Pang, Nicholas Francis Grigoropoulos, Bin Tean Teh, Qiang Yu, Soon Thye Lim, Wenyu Li, Choon Kiat Ong, Huiqiang Huang, Jing Tan
Summary: The study demonstrates that DLBCL patients with CREBBP deficiency respond well to the HDAC inhibitor chidamide, especially when combined with an AURKA inhibitor.
Article
Biochemistry & Molecular Biology
Elzbieta Wolny, Aleksandra Skalska, Agnieszka Braszewska, Luis A. J. Mur, Robert Hasterok
Summary: Salt stress affects germination and root growth in Brachypodium, influencing the expression of cell wall expansion-regulating proteins, induction of cell cycle genes, and alterations in genomic and epigenetic mechanisms such as histone acetylation and DNA methylation. These findings suggest a complex molecular response to salt stress in plants, indicating potential avenues for further functional genomics research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Yulong Li, Alexander J. Hartemink, David M. MacAlpine
Summary: This study investigated the dynamic changes in chromatin organization at and surrounding replication origins in Saccharomyces cerevisiae. It found that the downstream repositioning of the origin-proximal +1 nucleosome and an increase in protected DNA fragments spanning the ARS consensus sequence are indicative of pre-RC assembly. The strongest correlation between chromatin occupancy at the ACS and origin efficiency occurred in early S phase, consistent with the rate-limiting formation of the CMG complex being a determinant of origin activity. Nucleosome disruption and disorganization were observed emanating from replication origins and traveling with the elongating replication forks in S phase, reflecting the disassembly and assembly of chromatin ahead of and behind the replication fork.
Article
Chemistry, Multidisciplinary
Zhaoliang Yu, Peng Deng, Yufeng Chen, Shini Liu, Jinghong Chen, Zihuan Yang, Jianfeng Chen, Xinjuan Fan, Peili Wang, Zerong Cai, Yali Wang, Peishan Hu, Dezheng Lin, Rong Xiao, Yifeng Zou, Yan Huang, Qiang Yu, Ping Lan, Jing Tan, Xiaojian Wu
Summary: Dysregulation of the cell cycle machinery is a hallmark of colorectal cancer, with aberrant PLK1 signaling associated with recurrence and poor prognosis. Blocking PLK1 genetically and pharmacologically increases sensitivity to oxaliplatin. CDC7 is identified as a critical downstream effector of PLK1 signaling and its inhibitor enhances the anti-tumor effect of oxaliplatin in CRC models.
Review
Biochemistry & Molecular Biology
Crisanto Gutierrez
Summary: Cell division cycle is a fundamental process for the perpetuation of organisms, including plants. Studies on plant cell division cycle started with cytological and physiological approaches in the 1950s and 1960s, and later experienced rapid growth with the development of genetics and genomics. Current research not only focuses on understanding the plant cell cycle machinery, but also examines the interaction of cell cycle components with various aspects of plant biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Mohd Shariq, Neha Quadir, Anwar Alam, Sheeba Zarin, Javaid A. Sheikh, Neha Sharma, Jasmine Samal, Uzair Ahmad, Indu Kumari, Seyed E. Hasnain, Nasreen Z. Ehtesham
Summary: Intracellular pathogens exploit the cellular ubiquitination process to evade host immunity. Ubiquitin modification, as a way to regulate cellular functions, is used by pathogens to subvert host defenses. The hijacking of host ubiquitination system allows pathogens to escape immune responses and enhance their virulence by manipulating host autophagy.
Article
Multidisciplinary Sciences
Chunrui Xu, John J. Tyson, Yang Cao
Summary: The bacterium Caulobacter crescentus undergoes an asymmetric division into a stalked cell and a flagellated cell. This asymmetry is driven by the appearance of scaffolding proteins that recruit enzyme complexes to regulate the transcription factor CtrA. Our study uses a Turing-pattern mechanism to simulate the dynamics of these scaffolding proteins and their influence on CtrA abundance and distribution. The mathematical model captures key features of wild-type and mutant strains and predicts the distributions of CtrA and signaling proteins in mutants, providing insights into polar morphogenesis and asymmetry during the cell cycle in Caulobacter.
Article
Multidisciplinary Sciences
Jonathan M. Pojer, Samuel A. Manning, Benjamin Kroeger, Shu Kondo, Kieran F. Harvey
Summary: The Hippo pathway plays a critical role in regulating organ growth and cell fate, specifically in the context of R8 photoreceptor cells. The study reveals important differences in the expression and localization of key proteins in the Hippo pathway between R8 cells and growing epithelial organs, shedding light on the complex signaling network involved in organ growth and cell fate decision.
Article
Cell Biology
Jirarat Poohadsuan, George A. O'Doherty, Weerapat Owattanapanich, Smith Kungwankiattichai, Yon Rojanasakul, Surapol Issaragrisil, Sudjit Luanpitpong
Summary: Cardiac glycosides show significant therapeutic effects on LSCs in AML, inducing apoptosis independent of cell differentiation status. The cell cycle distribution and intrinsic apoptotic regulatory mechanisms of LSCs are crucial factors determining the sensitivity to cardiac glycosides.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Cell Biology
Antoine Simoneau, Rosalinda Xiong, Lee Zou
Summary: PARP inhibitor (PARPi) generates DNA double-strand breaks (DSBs) predominantly in a trans cell cycle manner by inducing single-stranded DNA (ssDNA) gaps during the first S phase after exposure. BRCA1/2-deficient cells are unable to effectively repair DSBs, resulting in the unique efficacy of PARPi in these cells.
GENES & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Monika Pioro, Tomasz Malecki, Magda Portas, Izabela Magierowska, Damian Trojanowski, David Sherratt, Jolanta Zakrzewska-Czerwinska, Katarzyna Ginda, Dagmara Jakimowicz
MOLECULAR MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Lukasz Makowski, Damian Trojanowski, Rob Till, Carey Lambert, Rebecca Lowry, R. Elizabeth Sockett, Jolanta Zakrzewska-Czerwinska
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Microbiology
Marcin Jan Szafran, Martyna Gongerowskal, Tomasz Malecki, Marie Elliott, Dagmara Jakimowicz
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Damian Trojanowski, Marta Kolodziej, Joanna Holowka, Rolf Mueller, Jolanta Zakrzewska-Czerwinska
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Review
Microbiology
Marcin J. Szafran, Agnieszka Strzalka, Dagmara Jakimowicz
Article
Microbiology
Malgorzata Plachetka, Dorota Zyla-Uklejewicz, Christoph Weigel, Rafal Donczew, Magdalena Donczew, Dagmara Jakimowicz, Anna Zawilak-Pawlik, Jolanta Zakrzewska-Czerwinska
Review
Microbiology
Monika Pioro, Dagmara Jakimowicz
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Lukasz Makowski, Damian Trojanowski, Jolanta Zakrzewska-Czerwinska
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Multidisciplinary Sciences
Marta Kolodziej, Damian Trojanowski, Katarzyna Bury, Joanna Holowka, Weronika Matysik, Hanna Kakolewska, Helge Feddersen, Giacomo Giacomelli, Igor Konieczny, Marc Bramkamp, Jolanta Zakrzewska-Czerwinska
Summary: Deletion of the lsr2 gene significantly impacts the cell morphology of M. smegmatis, resulting in cells that are shorter, wider, and more rigid than the wild-type cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Aleksandra Kowalczyk, Agata Paneth, Damian Trojanowski, Piotr Paneth, Jolanta Zakrzewska-Czerwinska, Pawel Staczek
Summary: Compounds targeting bacterial topoisomerases have been developed as potential antibacterial agents. Studies have shown that small-molecular weight thiosemicarbazides can act as gyrase and topoisomerase IV inhibitors. These compounds reduce the ATP hydrolysis ability of the ParE subunit of Staphylococcus aureus topoisomerase IV and exhibit better antibacterial activity against clinical strains of S. aureus. Additionally, compound 7 has been shown to prolong the replication process duration in Mycobacterium smegmatis and induce growth arrest of bacterial cells, making it a potential novel group of bacterial topoisomerase inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Martyna Gongerowska-Jac, Marcin Jan Szafran, Dagmara Jakimowicz
Summary: This study introduces a novel approach to identify regulatory proteins that control transcription in Streptomyces. The experimental results demonstrate that the SCO4804 gene positively influences the transcription of topA, revealing a new player in the control of chromosome topology in these bacteria.
MICROBIAL CELL FACTORIES
(2021)
Correction
Multidisciplinary Sciences
Marcin J. Szafran, Tomasz Malecki, Agnieszka Strzalka, Katarzyna Pawlikiewicz, Julia Dulawa, Anna Zarek, Agnieszka Kois-Ostrowska, Kim C. Findlay, Tung B. K. Le, Dagmara Jakimowicz
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Marcin J. Szafran, Tomasz Malecki, Agnieszka Strzalka, Katarzyna Pawlikiewicz, Julia Dulawa, Anna Zarek, Agnieszka Kois-Ostrowska, Kim C. Findlay, Tung B. K. Le, Dagmara Jakimowicz
Summary: Bacteria of the genus Streptomyces have a linear chromosome and undergo substantial rearrangement during sporulation, transitioning from an 'open' to a 'closed' conformation, similar to eukaryotes.
NATURE COMMUNICATIONS
(2021)
Review
Microbiology
Marcin J. Szafran, Dagmara Jakimowicz, Marie A. Elliot
FEMS MICROBIOLOGY REVIEWS
(2020)