Article
Immunology
Mona Mohamed Al-Shamiri, Sirui Zhang, Peng Mi, Yuqing Liu, Meng Xun, E. Yang, Li Ai, Lei Han, Yanjiong Chen
Summary: Acinetobacter baumannii is a significant pathogen in clinical settings, exhibiting high levels of multidrug-resistance and biofilm formation ability. Research found a strong correlation between antibiotic resistance and biofilm formation, while no correlation was detected between twitching motility and antibiotic susceptibility or biofilm formation. The timing of examination was highlighted as a key factor in determining biofilm formation ability.
MICROBIAL PATHOGENESIS
(2021)
Article
Infectious Diseases
Ola A. Abd El-Rahman, Fatma Rasslan, Safaa S. Hassan, Hossam M. Ashour, Reham Wasfi
Summary: MDR Acinetobacter baumannii is a critical pathogen in healthcare-associated infections, with its ability to form biofilms and transfer antibiotic resistance genes. The role of the RND efflux pump in biofilm formation was studied, and while it did not appear to be pivotal, the expression varied among different strains.
Review
Medicine, General & Internal
Subhasree Roy, Goutam Chowdhury, Asish K. Mukhopadhyay, Shanta Dutta, Sulagna Basu
Summary: Acinetobacter baumannii is a leading cause of nosocomial infections due to its ability to acquire antibiotic resistance genes and form biofilms. This review discusses the evolution of antibiotic resistance, mechanisms of biofilm formation, infections associated with A. baumannii colonization on medical devices, and strategies for preventing biofilm formation.
FRONTIERS IN MEDICINE
(2022)
Article
Microbiology
Morgane Choquet, Elodie Lohou, Etienne Pair, Pascal Sonnet, Catherine Mullie
Summary: The study evaluated the overexpression of efflux pumps in ciprofloxacin-resistant Acinetobacter baumannii strains, with most genes belonging to the RND family. Synthesis of 1-(1-naphthylmethyl)piperazine analogs as potential efflux pump inhibitors showed that quinoline and pyridine analogs were more effective, with stereochemistry playing a key role in inhibitory activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
K. Lucassen, S. Gerson, K. Xanthopoulou, J. Wille, T. Wille, H. Seifert, P. G. Higgins
Summary: In this study, the regulatory and expression patterns of AdeABC in reference strains ATCC 17978 and ATCC 19606 were compared, with ATCC 19606 demonstrating significantly stronger efflux activity. An amino acid substitution at residue 172 of adeS was identified as a potential cause for the differential expression of the pump. Therefore, caution is recommended when relying solely on single reference strains for research purposes.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Yanpeng Zhang, Bing Fan, Yong Luo, Zhiyuan Tao, Yongbo Nie, Yongtao Wang, Fanglin Ding, Yanwu Li, Dayong Gu
Summary: Carbapenem non-susceptible A. baumannii (CnSAB) strains showed increased distribution of carbapenemase genes and overexpression of RND family efflux pumps, leading to stronger antibiotic resistance, while carbapenem susceptible A. baumannii (CSAB) strains exhibited higher biofilm formation potential to survive antibiotic stress.
BMC INFECTIOUS DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Ebrahim Rafiei, Milad Shahini Shams Abadi, Behnam Zamanzad, Abolfazl Gholipour
Summary: The study found that multidrug-resistant A. baumannii isolates are resistant to Ciprofloxacin and Trimethoprim-sulfamethoxazole but more sensitive to Tigecycline. The frequency of efflux pump genes in these resistant strains is high, indicating that efflux pumps are one of the antibiotic resistance factors of A. baumannii.
Article
Electrochemistry
Kayode Olaifa, Obinna Ajunwa, Enrico Marsili
Summary: Drug repurposing is an immediate approach to fight antibiotic resistant pathogens, and antifungal drugs are good candidates for it. This study determined the activity of two antifungal drugs against Acinetobacter baumannii biofilms using bioelectrochemical methods and provided the basis for implementing these methods in clinical antimicrobial testing.
ELECTROCHIMICA ACTA
(2022)
Article
Microbiology
Zeeshan Taj, Muhammad Hidayat Rasool, Mohsin Khurshid, Bilal Aslam, Muhammad Usman Qamar
Summary: This study aims to assess the antibiotic and biocide susceptibility of Acinetobacter baumannii strains from five hospitals in Pakistan, as well as the impact of efflux pump inhibitors on antimicrobial susceptibility and the distribution of ARGs and STs. The findings indicate that A. baumannii strains in Pakistan are resistant to antibiotics and may be developing biocide resistance, which could contribute to the selection and dissemination of multi-drug-resistant strains.
Review
Immunology
Chunli Sun, Yunsong Yu, Xiaoting Hua
Summary: Acinetobacter baumannii is a common pathogen causing various infectious diseases and has a high drug resistance rate, severely limiting antibiotic treatment options. Tigecycline and polymyxins are considered the last line of defense against multidrug resistant A. baumannii. This review focuses on the mechanisms of tigecycline resistance in A. baumannii and highlights the need for further investigation and development of new antibiotics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Melvin Roberto Tapia-Rodriguez, Ernesto Uriel Cantu-Soto, Francisco Javier Vazquez-Armenta, Ariadna Thalia Bernal-Mercado, Jesus Fernando Ayala-Zavala
Summary: This study evaluated the efficacy of oregano essential oil and its constituents in inhibiting Acinetobacter baumannii biofilms. The results showed that the treatment had a significant reduction in biofilm biomass and covered area, as well as reduced bacterial viability and twitching motility. This suggests that terpenes could be effective agents against A. baumannii biofilms and provide potential strategies against persistent infections and antibiotic resistance.
Editorial Material
Microbiology
Santiago Castillo-Ramirez
Summary: A recent study published in mBio sequenced the genomes of 313 carbapenem-resistant A. baumannii isolates from over 100 hospitals in almost 50 countries, providing an updated view of the global distribution of major international clones and their carbapenemase genes. Future global genomic epidemiology studies can be enhanced by considering both human and non-human isolates and their antibiotic resistance profiles.
Article
Immunology
Neveen M. Saleh, Sarah I. Saad, Mohamed El-Sayed, Gharieb S. El-Sayyad, Feriala A. Abo Safe
Summary: The overuse of antibiotics for infection treatment has led to the rapid spread of multi-drug resistance Acinetobacter baumannii in hospitals. In our study, we evaluated the contribution of aminoglycoside resistance mechanisms in A. baumannii to the resistance surge in selected Egyptian hospitals. The analysis revealed a multidrug-resistant pattern and limited susceptibilities to aminoglycosides, highlighting the alarming levels of aminoglycoside resistance in A. baumannii isolates.
MICROBIAL PATHOGENESIS
(2023)
Review
Infectious Diseases
Alemu Gedefie, Wondmagegn Demsis, Melaku Ashagrie, Yeshimebet Kassa, Melkam Tesfaye, Mihret Tilahun, Habtye Bisetegn, Zenawork Sahle
Summary: Acinetobacter species, especially A. baumannii, pose a significant threat in hospital environments, particularly in ICUs, due to their ability to develop antibiotic resistance mechanisms and cause biofilm-associated infections. Health care workers should focus on infection prevention and selective treatments to combat biofilm-related infections caused by A. baumannii.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Johanna Ruehl-Teichner, Lisa Jacobmeyer, Ursula Leidner, Torsten Semmler, Christa Ewers
Summary: This study investigated 78 isolates of Acinetobacter baumannii from horses and found that they displayed high genetic diversity, the ability to form biofilms, and resistance to multiple antimicrobial drugs. The presence of globally distributed dominant clones was also identified. These findings are important for understanding treatment failures and limited availability of licensed drugs in equine medicine.
Article
Infectious Diseases
Marion Nicol, Mohamed Amine Ben Mlouka, Thierry Berthe, Patrick Di Martino, Thierry Jouenne, Jean-Michel Brunel, Emmanuelle De
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2019)
Article
Materials Science, Biomaterials
Naila Bou Haidar, Stephane Marais, Emmanuelle De, Annick Schaumann, Magalie Barreau, Marc G. J. Feuilloley, Anthony C. Duncan
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Estelle Deschamps, Annick Schaumann, Isabelle Schmitz-Afonso, Carlos Afonso, Emmanuelle De, Corinne Loutelier-Bourhis, Stephane Alexandre
Summary: This study investigated the membrane phospholipids of Pseudomonas aeruginosa grown in CF sputum-like media, showing that the growth medium can influence its membrane lipid composition and antibiotic resistance, especially when P. aeruginosa uses DOPC in SCFM-PC. Variation in phospholipid quantity was observed depending on the growth medium used, with no significant impact on bacterial growth and shape.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Microbiology
Alizee Guerin, Sheiam Sulaeman, Laurent Coquet, Armelle Menard, Frederique Barloy-Hubler, Emmanuelle De, Odile Tresse
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Hung Le, Christophe Arnoult, Emmanuelle De, Damien Schapman, Ludovic Galas, Didier Le Cerf, Carole Karakasyan
Summary: Conventional antibiotic treatments often fail to eradicate biofilm-related infections, but novel methods of antibiotic delivery such as targeting nanoparticulate systems show promise in enhancing bactericidal activity against commonly virulent pathogens like Staphylococcus aureus. Studies have demonstrated improved therapeutic efficacy and elevated accumulation of antibiotics in biofilms using targeting nanoparticle systems, suggesting a potential strategy to enhance antibiotic biodistribution and efficacy.
Article
Biotechnology & Applied Microbiology
Meriem Zaidi-Ait Salem, Yassine Nait Chabane, Elisabeth Girbal-Neuhauser
Summary: This paper investigates the ability of Bacillus amyloliquefaciens strain L-17 to form subaerial biofilms on a liquid surface, revealing a complex structure mainly composed of polysaccharides and proteins. The rough top biofilm layer in contact with air showed hydrophobic properties, while the smooth biofilm layer in contact with liquid exhibited hydrophilic properties.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Chemistry, Applied
Yuzhen Lou, Damien Schapman, Dimitri Mercier, Stephane Alexandre, Emmanuelle De, Jean-Michel Brunel, Nasreddine Kebir, Pascal Thebault
Summary: Poly(dimethyl siloxane) (PDMS) is a widely used material in the biomedical field, but its hydrophobic character makes it prone to bacterial adhesion and biofilm formation. By covalently grafting an azidated claramine-derivative onto a modified PDMS surface, the modified surface showed antibacterial activity against both Gram - (Escherichia coli) and Gram + (Staphyloccocus epidermidis) bacteria.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Microbiology
Brandon Robin, Marion Nicol, Hung Le, Ali Tahrioui, Annick Schaumann, Jean-Baptiste Vuillemenot, Delphine Vergoz, Olivier Lesouhaitier, Thierry Jouenne, Julie Hardouin, Anais Potron, Valerie Perrot, Emmanuelle De
Summary: Acinetobacter baumannii is a problematic bacterial pathogen that is resistant to antibiotics and can form biofilms. The MacAB-TolC system is involved in biofilm formation in A. baumannii.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Sebastien Massier, Brandon Robin, Marianne Megroz, Amy Wright, Marina Harper, Brooke Hayes, Pascal Cosette, Isabelle Broutin, John D. Boyce, Emmanuelle De, Julie Hardouin
Summary: Acinetobacter baumannii is a problematic nosocomial pathogen due to its antibiotic resistance and ability to form biofilms. The study identified phosphoproteins in two strains cultured in different growth modes, with biofilm modes showing more phosphorylation sites and proteins. The research suggests potential therapeutic targets against this pathogen.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Hung Le, Carole Karakasyan, Thierry Jouenne, Didier Le Cerf, Emmanuelle De
Summary: Antibiotic delivery based on polymeric nanoparticles is a potential method to enhance antibiotic treatment, improve pharmacokinetic properties, extend the antimicrobial spectrum, reduce required dosage, and preserve efficacy. This technology offers a steady solution by continuously developing novel molecules and incorporating them into the delivery platform.
APPLIED SCIENCES-BASEL
(2021)
Article
Multidisciplinary Sciences
Meriem Zaidi-Ait Salem, Elisabeth Girbal-Neuhauser, Yassine Nait Chabane
Summary: The Bacillus amyloliquefaciens L-17 strain was isolated from chicken feathers, and its genome size is 3,933,788 bp with 4001 coding sequences. The BioProject has been deposited at NCBI GenBank with specific accession numbers.
Review
Biotechnology & Applied Microbiology
Tassadit Ouidir, Bruno Gabriel, Yassine Nait Chabane
Summary: Biofilms, composed of bacteria in a sessile state within an extracellular matrix, are studied for their impact on various aspects of daily life. While the majority of research has focused on single-species biofilms, recent studies have shifted towards complex models that better approximate the natural environment. This review discusses examples of multispecies biofilms in different domains and highlights the need for multi-scale strategies to decipher this complex lifestyle.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Delphine Vergoz, Flore Nilly, Florie Desriac, Magalie Barreau, Antoine Gery, Charlie Lepetit, Francois Sichel, Katy Jeannot, Jean-Christophe Giard, David Garon, Sylvie Chevalier, Cecile Muller, Emmanuelle De, Jean Michel Brunel
Summary: A series of 6-polyaminosteroid analogues of squalamine were synthesized and evaluated for their antimicrobial properties against susceptible and resistant bacteria strains. The compounds showed promising activity against Gram-positive bacteria and strong activity against resistant Gram-negative bacteria. These analogues have the potential to be used as treatments for Gram-positive bacterial infections and as adjuvants to combat Gram-negative bacterial resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Nicolas Nalpas, Takfarinas Kentache, Emmanuelle De, Julie Hardouin
Summary: Through proteomic analysis, we identified 84 K-trimethylated proteins in Acinetobacter baumannii for the first time, which is of great importance to the scientific community.
JOURNAL OF PROTEOME RESEARCH
(2023)
Editorial Material
Microbiology
Raffaele Zarrilli, Paolo A. Visca, Remy A. Bonnin, Emmanuelle De
FRONTIERS IN MICROBIOLOGY
(2023)