Review
Infectious Diseases
Bruno Viaggi, Alice Cangialosi, Martin Langer, Carlo Olivieri, Andrea Gori, Alberto Corona, Stefano Finazzi, Antonello Di Paolo
Summary: The clinical outcome of severe infections in ICU patients depends on multiple factors, including early administration of chemotherapies and comorbidities. Different antibacterial drugs have variable tissue penetration rates, and the correlation between plasma and tissue concentrations may be inconsistent. This review focuses on antibacterial drugs that act as protein synthesis inhibitors and disrupt DNA structure and function. It found that fluoroquinolones, macrolides, linezolid, and tigecycline have excellent diffusion into epithelial lining fluid, which is crucial for the treatment of ventilator and healthcare-associated pneumonia. Some drugs also show high penetration rates in cerebrospinal fluid, skin, and soft tissues. Further studies are needed to improve understanding of drug tissue penetration, especially in the presence of factors that may affect drug pharmacokinetics.
Review
Immunology
Annie S. Jasper, Jackson S. Musuuza, Jessica S. Tischendorf, Vanessa W. Stevens, Shantini D. Gamage, Fauzia Osman, Nasia Safdar
Summary: Comparing fluoroquinolone versus macrolide monotherapy in Legionella pneumonia, no significant difference was found in reducing mortality rates, clinical cure, time to apyrexia, length of hospital stay, and occurrence of complications.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Chemistry, Medicinal
Ravleen Kaur, Pooja Rani, Atanas G. Atanasov, Qushmua Alzahrani, Reena Gupta, Bhupinder Kapoor, Monica Gulati, Pooja Chawla
Summary: Antibacterial drug resistance has become a significant public health issue, leading to the exploration of new molecules. Many new molecules are currently in clinical trials and some have been commercialized.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2022)
Article
Otorhinolaryngology
Joey Siu, Brett Wagner Mackenzie, Lilian Klingler, Kristi Biswas, Yi Wang, Cheung-Tak Hung, Soo Hee Jeong, Daniel Barnett, Malcolm Drummond Tingle, Richard George Douglas
Summary: The study found no significant changes in bacterial community composition in sinus samples following antibiotic treatment, but a trend of decreased gut microbial diversity. In the group receiving roxithromycin, sinus bacterial diversity was negatively correlated with serum drug levels, and the relative abundance of Staphylococcus ASV129 was reduced as mucus doxycycline levels increased. Further investigation into antibiotic prescription for CRS is needed due to limited sinonasal drug penetration, uncertain efficacy, and potential dysbiosis impact.
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2021)
Article
Multidisciplinary Sciences
Mohammad I. Al-Wabel, Munir Ahmad, Jahangir Ahmad, Nahrir M. A. Lubis, Adel R. A. Usman, Abdullah S. F. Al-Farraj
Summary: Application of manure and compost can lead to accumulation of veterinary antibiotics in soil, posing ecological risks. This study investigated the occurrence and potential ecological risk assessment of nine antibiotics residues in soil, manure, and compost samples from Al-Kharj, Saudi Arabia. While most studied antibiotics showed low risk quotient values, oxytetracycline and doxycycline had higher values, indicating possible adverse ecological effects in soil.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Dandara Cassu-Corsi, Fernanda F. Santos, Rodrigo Cayo, Willames M. B. S. Martins, Carolina S. Nodari, Luiz G. P. Almeida, Rafael A. Martins, Roberto J. Carvalho da Silva, Ana Tereza R. Vasconcelos, Antonio C. C. Pignatari, Ana C. Gales
Summary: This study sequenced Neisseria gonorrhoeae isolates collected in Sao Paulo, Brazil and identified different sequence types and resistance mutations. The results contribute to the understanding of N. gonorrhoeae strains circulating in the region.
Article
Biochemical Research Methods
Binila K. Korah, Anu Rose Chacko, Sneha Mathew, Bony K. John, Thomas Abraham, Beena Mathew
Summary: A novel carbon dot material was synthesized using a facile method and characterized using various techniques. The carbon dots exhibited high stability and good fluorescence properties, and were successfully used for detecting antibiotics.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Ferdos Kord Mostafapour, Mohammadreza Radmehr, Shaziya Haseeb Siddiqui, Davoud Balarak
Summary: This study aims to investigate the removal efficiency of ciprofloxacin (CFX) from aqueous solutions using the sonophotocatalytic process of Magnesium Oxide Nanoparticles (MgO). The results showed that the process of photosonocatalysis with MgO can effectively lead to the removal of the antibiotic CFX.
INTERNATIONAL JOURNAL OF PHARMACEUTICAL INVESTIGATION
(2023)
Article
Microbiology
Martijn Zwama, Kunihiko Nishino
Summary: Recent mutations in RND efflux pumps have increased multidrug resistance. R717L and R717Q substitutions in the AcrB pump of Escherichia coli dramatically enhance resistance to macrolides and fluoroquinolones, while rendering cells more susceptible to novobiocin and cloxacillin. We emphasize the need for antibiotic control, adjustment of treatments, and the development of novel antibiotics and efflux pump inhibitors.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Yawen Guo, Zhaoyuan He, Jinyuan Chen, Lan Chen, Kaizhou Xie, Tao Zhang, Genxi Zhang, Guojun Dai
Summary: An innovative method for simultaneous determination of three tetracycline and two fluoroquinolone residues in poultry eggs was established using UPLC-FLD. The optimized method showed high recoveries above 83.5% and precision ranging from 1.99% to 6.24%, meeting EU and U.S. FDA regulations. The proposed method demonstrated reliability and applicability in quantitative analyses of target drug residues in egg samples.
Article
Immunology
Yunyu Tang, Xiaoyi Lou, Guangxin Yang, Liangliang Tian, Yuan Wang, Xuanyun Huang
Summary: This study investigated the occurrence of antibiotics and potential health risks in 300 cultured fish samples from 19 provinces in China. The results showed a detection frequency of 24.3% for antibiotics, with high concentrations found in Shandong province. Despite the high detection frequency and levels of antibiotics in the samples, the ingestion of cultured fish did not pose significant health risks to human according to estimated daily intakes and hazard quotients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biology
Alexis J. Vega, Caitlin Smith, Hannah Grace Matejowsky, Katherine J. Thornhill, Grant E. Borne, Chizoba N. Mosieri, Sahar Shekoohi, Elyse M. Cornett, Alan D. Kaye
Summary: The administration of warfarin poses a challenge due to increased patient susceptibility to major bleeding, especially when co-administered with antibiotics capable of modulating its metabolism. Evidence consistently supports an elevated risk of bleeding in patients receiving antibiotics and warfarin therapy. This review highlights the importance of diligent monitoring in patients on warfarin requiring antibiotic therapy and the potential benefits of dose reduction or adjustment for certain antibiotics.
Article
Chemistry, Applied
Zhaoyuan He, Yawen Guo, Lan Chen, Kaizhou Xie, Yang Lu, Jinyuan Chen, Genxi Zhang, Tao Zhang, Guojun Dai
Summary: A sensitive and efficient UPLC-FLD method was developed for detecting TC, OTC, DOX, ENR, and CIP residues in chicken muscle. The method involved liquid-liquid extraction and solid-phase extraction for sample preparation, followed by chromatographic separation using an ACQUITY UPLC BEH C18 column. The method showed good recovery rates and low RSD values, and was successfully applied to commercial chicken samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Microbiology
Jade Bokma, Nick Vereecke, Hans Nauwynck, Freddy Haesebrouck, Sebastiaan Theuns, Bart Pardon, Filip Boyen
Summary: This study utilized rapid long-read sequencing to generate high-quality genomes of 100 M. bovis isolates and conducted a GWAS analysis to investigate natural and acquired antimicrobial resistance, identifying potential genetic markers associated with resistance.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Carolina Cruz, Lucia Rodrigues, Filipa Fernandes, Ricardo Santos, Paulo Paixao, Maria Jesus Chasqueira
Summary: This study aimed to describe the antibiotic susceptibility pattern of environmental Legionella recovered in the south of Portugal. The results showed that fluoroquinolones were the most active antibiotics, while doxycycline was the least sensitive. The minimal inhibitory concentration distributions for all antibiotics were higher than those reported by EUCAST. Interestingly, two phenotypically resistant isolates with high-level quinolone resistance were identified. This is the first investigation of MIC distributions, lpeAB, and tet56 genes in Portuguese environmental isolates of Legionella.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Antonia Ertelt, Roswitha Merle, Friederike Stumpff, Lena Bollinger, Sarah Liertz, Corinna Weber, Heidrun Gehlen
Summary: The study aimed to assess changes in blood parameters before and after a 160 km endurance race and evaluate differences in cardiac biomarkers between finishers and non-finishers among 52 healthy endurance horses. Results showed significant increases in lactate dehydrogenase, SDMA, and ADMA after the race in the finisher group. Furthermore, cardiac troponin I and alpha-HBDH concentrations increased significantly post-race in both finishers and gait-related groups.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Heidrun Gehlen, Julia Puhlmann, Roswitha Merle, Christa Thone-Reineke
Summary: The study found that most horse owners used auxiliary reins responsibly, but there is still a need for further clarification on the correct application regarding animal welfare and training physiology. While most participants had some understanding of the correct head position of the horse, there were still too many who fastened the reins too tightly, leading to breathing problems that were not addressed during training.
Article
Agriculture, Dairy & Animal Science
Melanie Humpenoeder, Giuliano M. Corte, Marcel Pfuetzner, Mechthild Wiegard, Roswitha Merle, Katharina Hohlbaum, Nancy A. Erickson, Johanna Plendl, Christa Thoene-Reineke
Summary: Simulators for laboratory animal training have the potential to address the dilemma between the demand for practical training involving live animals and implementing the 3R principle. While awareness of existing simulators is high, their implementation is low, indicating a need for user-optimized, realistic, financially affordable, and robust rat and mouse simulators.
Article
Agriculture, Dairy & Animal Science
Gudrun Haase, Mechthild Wiegard, Christa Thoene-Reineke, Katrin Baumgartner, Lorenzo von Fersen, Hermann Will, Roswitha Merle, Manel Lopez-Bejar, Oriol Tallo-Parra, Annais Carbajal, Lukas Reese
Summary: The flight restriction of great white pelicans does not seem to have a significant impact on welfare indicators, adrenal activity, or behavior assessed in this study. However, reversibly deflighted pelicans showed higher levels of stress indicator CORTf compared to irreversibly deflighted and airworthy pelicans. Additionally, group size of the pelicans may influence their welfare, with larger groups having lower stress levels.
Article
Multidisciplinary Sciences
Andreas W. Oehm, Roswitha Merle, Annegret Tautenhahn, K. Charlotte Jensen, Kerstin-Elisabeth Mueller, Melanie Feist, Yury Zablotski
Summary: Lameness is a significant issue in intensively managed dairy herds globally, with negative impacts on animal welfare and economic viability. Factors such as low body condition, elevated somatic cell count, severe hock lesions, increasing parity, absence of pasture access, and poor udder cleanliness are associated with higher locomotion scores. Additionally, breed, observer, and season also influence locomotion scores. The use of locomotion scores instead of a dichotomised response variable provides a more detailed understanding of the complex nature of gait disturbances in dairy cows.
Article
Agriculture, Dairy & Animal Science
Marielu Voit, Katrin Baumgartner, Lorenzo von Fersen, Roswitha Merle, Lukas Reese, Mechthild Wiegard, Hermann Will, Oriol Tallo-Parra, Annais Carbajal, Manel Lopez-Bejar, Christa Thoene-Reineke
Summary: The study compared two methods, plucking and cutting feathers, for determining corticosterone levels in feathers. Results showed no significant differences in corticosterone levels between the two methods for both species, indicating that cutting feathers is a valid alternative sampling method.
Article
Infectious Diseases
Marianne Moerer, Roswitha Merle, Wolfgang Baeumer
Summary: The amendment to regulations of veterinary pharmacies in Germany has had a positive impact on the use of antibiotics in dogs and cats. The results of an online survey showed a decrease in the use of highest priority critically important antimicrobials and overall antimicrobial use. Veterinarians are requesting antimicrobial susceptibility testing more frequently and opting for alternative antimicrobials in treatment.
Article
Chemistry, Multidisciplinary
Nina Langkabel, Verena Oswaldi, Roswitha Merle, Janine Dzierzon, Diana Meemken
Summary: The objective of this study was to determine the effect of freezing broiler neck skin samples on their microbial analysis. The results showed that there was no significant difference between frozen and fresh samples in terms of total viable count and Enterobacteriaceae count. Therefore, freezing broiler neck skin samples can be considered as a viable option for microbiological examination.
APPLIED SCIENCES-BASEL
(2022)
Article
Agriculture, Dairy & Animal Science
Dagmar S. Trachsel, Hannah J. Stage, Sebastian Rausch, Susanne Trappe, Katharina Sollig, Gerhard Sponder, Roswitha Merle, Jorg R. Aschenbach, Heidrun Gehlen
Summary: This study explores the potential of adipose tissue-derived stem cells in the differentiation into heart cells in horses. By comparing different isolation methods from various adipose tissue sources, it was found that abdominal adipose tissue is suitable for isolating equine stem cells. However, unlike in other species, equine stem cells showed resistance to the induction of heart cell differentiation.
Article
Agriculture, Dairy & Animal Science
Sabita Diana Stoeckle, Detlef Timmermann, Roswitha Merle, Heidrun Gehlen
Summary: Laminitic horses commonly suffer from an endocrine disease such as equine metabolic syndrome, with hyperinsulinemia considered a key factor. Resting plasma amino acid concentrations differed significantly between obese horses and those with insulin dysregulation and laminitis, suggesting the potential for diagnostic testing and feed supplements for horses at risk of developing laminitis.
Article
Agriculture, Dairy & Animal Science
Sabita Diana Stoeckle, Detlef Timmermann, Roswitha Merle, Heidrun Gehlen
Summary: Pituitary pars intermedia dysfunction (PPID) is a common disease in aged horses and ponies, involving oxidative damage to dopaminergic pathways. Changes in serum amino acid profiles may be potential diagnostic markers and interesting research approaches in PPID, similar to Parkinson's disease in humans.
Article
Veterinary Sciences
Heidrun Gehlen, Katja-Sophia Klein, Roswitha Merle, Antina Luebke-Becker, Sabita D. Stoeckle
Summary: The role of indicator pathogens in equine surgical site infection (SSI) and other infection-promoting factors was evaluated through a cross-sectional study. The results showed that MDRPs were identified in almost 70% of the SSI, indicating that colonization with MDR pathogens is only one of the crucial factors for the development of SSI.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Hannah J. Stage, Susanne Trappe, Katharina Soellig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Joerg R. Aschenbach, Heidrun Gehlen
Summary: This study investigated the proliferation and multilineage differentiation potential of equine adipose stem cells (ASCs) obtained from different tissue sources. The study found that ASCs had high proliferation and differentiation potential, but were unable to undergo cardiomyogenic differentiation. This study provides a basis for future research in equine medicine.
Article
Infectious Diseases
Marianne Moerer, Antina Luebke-Becker, Astrid Bethe, Roswitha Merle, Wolfgang Baeumer
Summary: The aim of this study was to investigate the impact of the second amendment to the Regulation of Veterinary Pharmacies (TaHAV) in 2018 on the occurrence of antimicrobial resistance in selected bacterial pathogens isolated from dogs and cats in Germany. The study found that the amendment led to an increase in sample submissions in Germany. The highest resistance rates were found for Staphylococcus pseudintermedius, Staphylococcus aureus, and Escherichia coli, while resistance rates were low for Pasteurella multocida and β-hemolytic streptococci. Significant decreases in resistance rates were observed for S. pseudintermedius against penicillin G and ampicillin, S. felis against amoxicillin-clavulanic acid and cefovecin, and S. aureus against enrofloxacin.
Article
Veterinary Sciences
Heidrun Gehlen, Judith Fisch, Roswitha Merle, Dagmar S. Trachsel
Summary: This study found that treatment with pergolide did not affect the ventricular function nor induce valvular disease in a small population of horses with confirmed PPID.
JOURNAL OF VETERINARY SCIENCE
(2021)