Article
Microbiology
Pavel Alexyuk, Andrey Bogoyavlenskiy, Madina Alexyuk, Kuralay Akanova, Yergali Moldakhanov, Vladimir Berezin
Summary: Colibacillosis in chickens can lead to death, weight loss, and economic losses. Antibiotic therapy is currently the main treatment method, but the rise of antibiotic resistance highlights the need for alternative treatments that align with the One Health concept. Phage therapy meets these requirements.
Article
Multidisciplinary Sciences
Hussein M. Galal, M. I. Abdrabou, Ahmed H. I. Faraag, C. K. Mah, Azza M. Tawfek
Summary: The study demonstrates that administering Poulvac E. coli at seven days of age, along with Linco-Spectin 100 SP during the first three days, has produced the best results in terms of protection and performance in the ME high presence of avian pathogenic E. coli field challenge.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Akiyo Suga, Marina Kawaguchi, Tetsuro Yonesaki, Yuichi Otsuka
Summary: This study identified key amino acid residues important for the interaction between gp37 protein in the distal tip (DT) region of T4 long tail fibers and OmpC of Escherichia coli, shedding light on the mechanism underlying phage host specificity. The results suggest that the shapes of both proteins at the binding interface play crucial roles in their interactions. Multiple phage mutants capable of adsorbing to various E. coli receptors were successfully isolated using a mutant T4 phage library with artificial modifications in the DT region, providing a basis for altering host specificity in phage therapy development.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Lica Lozica, Kasper Romer Villumsen, Ganwu Li, Xiao Hu, Maja Mauric Maljkovic, Zeljko Gottstein
Summary: This study investigates the impact of an autogenous vaccine on Escherichia coli strains isolated from broiler breeder flocks on two farms. The results show that highly virulent sequence types have higher homogeneity, while less frequent sequence types are phylogenetically more distant and carry more antimicrobial resistance genes. The frequency of antimicrobial resistance genes decreases over time, and strains belonging to the commensal group have the highest prevalence.
Article
Microbiology
Marianne Nicolas, Angelina Trotereau, Antoine Culot, Arshnee Moodley, Robert Atterbury, Jeroen Wagemans, Rob Lavigne, Philippe Velge, Catherine Schouler
Summary: Colibacillosis, the most common bacterial disease affecting poultry, is primarily treated with antibiotics. However, the rise of multidrug-resistant avian-pathogenic Escherichia coli (APEC) necessitates the exploration of alternative treatments, such as phage therapy. This study isolates and characterizes 19 genetically diverse coliphages, and demonstrates their efficacy in controlling APEC infections in ovo.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Courtney A. Fancher, Hudson T. Thames, Mary Gates Colvin, Mercedes Smith, Alyssa Easterling, Nikhil Nuthalapati, Li Zhang, Aaron Kiess, Thu T. N. Dinh, Anuraj Theradiyil Sukumaran
Summary: The study investigated the prevalence and virulence of E. coli in antibiotic-free broiler farms, determining that there is a high prevalence of virulent E. coli, especially during the spring season. Antimicrobial resistance was highest for tetracycline, and serogroups O8 and O78 were most frequent in virulent E. coli.
MICROBIOLOGY SPECTRUM
(2021)
Article
Food Science & Technology
Rafael Tabla, Antonia Gomez, Jose Emilio Rebollo, Felipe Molina, Isidro Roa
Summary: This study found that using a cocktail of four selected coliphages can effectively inhibit the growth of E. coli in cheese and reduce early blowing of cheese. In addition to reducing E. coli growth at the beginning of fermentation, treatment with phages also significantly decreased the number of cheese eyes and the area occupied by gas holes in cheese slices during cheese ripening.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Microbiology
Inger Helene Kravik, Hakon Kaspersen, Siri Kulberg Sjurseth, Malin Jonsson, Bruce David, Marina Aspholm, Camilla Sekse
Summary: This study emphasizes the importance of developing reliable and cost-effective sampling guidelines for investigating colibacillosis in broiler flocks. Through whole genome sequencing and phylogenetic analysis, it was found that there was limited diversity between APEC isolates from different organs within individual birds. Furthermore, isolates from the liver consistently represented the major disease-causing APEC, even when multiple sequence types were detected within an individual bird and flock.
VETERINARY MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Timothy J. Johnson, Elizabeth A. Miller, Cristian Flores-Figueroa, Jeannette Munoz-Aguayo, Carol Cardona, Katie Fransen, Megan Lighty, Eric Gonder, Jill Nezworski, Adam Haag, Michelle Behl, Michelle Kromm, Ben Wileman, Marissa Studniski, Randall S. Singer
Summary: Colibacillosis in poultry is a unique disease caused by Escherichia coli, and its primary route of entry is through the respiratory tract of birds. This study reveals the high prevalence of APEC plasmid in clinical isolates from diseased turkeys, as well as in cecal E. coli isolates from healthy birds. Furthermore, it identifies dominant sequence types (STs) that are more virulent in clinical strains compared to cecal strains. A revised APEC screening tool is proposed, which improves the identification of high-risk APEC clones in poultry production systems.
Article
Agriculture, Dairy & Animal Science
Toshiyuki Murase, Hiroichi Ozaki
Summary: This study showed that the prevalence of phylogroup F was higher in colibacillosis-related isolates compared to healthy chicken-related isolates, while phylogroups A and B1 were more commonly found in the latter. APEC strains with virulence-associated genes were more prevalent in colibacillosis-related isolates, indicating that phylogroup F can predict colibacillosis in E. coli strains.
Article
Veterinary Sciences
Sofie Kromann, Sharmin Baig, Marc Stegger, Rikke Heidemann Olsen, Anders Miki Bojesen, Henrik Elvang Jensen, Ida Thofner
Summary: This study explored the lesions causing background mortality in broiler breeders and the diversity of Escherichia coli strains. It found that exudative peritonitis was the most frequent lesion linked to infectious disease, and different genetic lineages of E. coli were present in birds without apparent infections.
VETERINARY RESEARCH
(2022)
Article
Immunology
Lica Lozica, Celine Sadaf Morteza Gholi, Adaya Kela, Ivan Losic, Danijela Horvatek Tomic, Zeljko Gottstein
Summary: This article presents a case where a colibacillosis outbreak in a family farm of laying hens was successfully treated using an autogenous vaccine. The vaccine significantly reduced morbidity and mortality rates, and improved egg production.
Article
Multidisciplinary Sciences
Darby M. Newman, Nicolle L. Barbieri, Aline L. de Oliveira, Dajour Willis, Lisa K. Nolan, Catherine M. Logue
Summary: Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is a devastating disease in poultry industry, causing significant economic losses. Study found that APEC strains commonly carry virulence-associated genes and ColV plasmid genes, form biofilms, and exhibit antimicrobial resistance.
Article
Food Science & Technology
Khashayar Shahin, Hongduo Bao, Shujiao Zhu, Abbas Soleimani-Delfan, Tao He, Mojtaba Mansoorianfar, Ran Wang
Summary: This study identified and characterized three highly effective antibacterial phages with stable bacteriolytic activity under different conditions. These phages exhibited extensive lytic effects on a wide range of E. coli strains, including pathogenic and antibiotic-resistant ones. Food testing confirmed that the phage cocktail was able to completely inhibit the growth of E. coli in food matrices, making them ideal candidates for further development as bio-control agents for E. coli in food production.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Stanley Brown, Namiko Mitarai, Kim Sneppen
Summary: Bacteriophage A is a temperate virus that infects Escherichia coli. The protection provided by A lysogen against A-sensitive bacteria is sensitive to the growth state of the mixed culture and is dependent on bacterial energy metabolism. However, a mutant of the A tail protein, AJ, is not affected by these factors and retains its protective function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Maged El-Ashker, Mayada Gwida, Stefan Monecke, Fatma El-Gohary, Ralf Ehricht, Mona Elsayed, Paul Akinduti, Mohamed El-Fateh, Sven Maurischat
VETERINARY MICROBIOLOGY
(2020)
Article
Environmental Sciences
Mona M. Elsayed, Fatma A. Elgohary, Amira I. Zakaria, Rasha M. Elkenany, Ayman Y. EL-Khateeb
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Microbiology
Mayada Gwida, Stefanie Lueth, Maged El-Ashker, Amira Zakaria, Fatma El-Gohary, Mona Elsayed, Sylvia Kleta, Sascha Al Dahouk
Article
Agriculture, Dairy & Animal Science
Maged El-Ashker, Mayada Gwida, Stefan Monecke, Ralf Ehricht, Mona Elsayed, Fatma El-Gohary, Annett Reissig, Elke Mueller, Akinduti Paul, Etinosa O. Igbinosa, Abeni Beshiru, Sven Maurischat
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2020)
Article
Infectious Diseases
Fatma A. El-Gohary, Lina Jamil M. Abdel-Hafez, Amira Zakaria, Radwa Reda Shata, Amin Tahoun, Amany El-Mleeh, Eman A. Abo Elfadl, Ehab Kotb Elmahallawy
INFECTION AND DRUG RESISTANCE
(2020)
Article
Agriculture, Dairy & Animal Science
Fatma A. El-Gohary, Eman Zahran, Eman A. Abd El-Gawad, Adel H. El-Gohary, Fatma M. Abdelhamid, Amany El-Mleeh, Ehab Kotb Elmahallawy, Mona Mohieldin Elsayed
Article
Agriculture, Dairy & Animal Science
Marwa A. Ahmed, Ehab Kotb Elmahallawy, Ahmed Gareh, Abdelbaset Eweda Abdelbaset, Fatma A. El-Gohary, Nagwa M. Elhawary, Ahmed K. Dyab, Elzahara Elbaz, Mostafa F. N. Abushahba
Article
Biology
Samah M. Mosad, Mohamed El-Tholoth, Ali A. El-Kenawy, Lina Jamil M. Abdel-Hafez, Fatma A. El-Gohary, Hanem El-Sharkawy, Mona Mohieldin Elsayed, Ayman A. Saleh, Ehab Kotb Elmahallawy
Article
Agriculture, Dairy & Animal Science
Khaled Saad Abd Elfatah, Moshira Abas Elabasy, Faris El-khyate, Ehab Kotb Elmahallawy, Samah M. Mosad, Fatma A. El-Gohary, Walied Abdo, Ashraf Al-Brakati, Mohamed G. Seadawy, Abd Elnaby Tahoon, Abd Elgalil El-Gohary
Summary: Newcastle disease virus (NDV) is a highly contagious viral disease affecting various avian species, particularly chickens, with the sub-genotype VII.1.1 found in wild birds. Molecular and histopathological methods play crucial roles in detecting and characterizing the virus. Further research is needed to explore the epidemiology and strains of NDV in different bird species.
Review
Biochemistry & Molecular Biology
Ehab Kotb Elmahallawy, Yasser Mohamed, Walied Abdo, Fatma A. El-Gohary, Shaimaa Ahmed Awad Ali, Tokuma Yanai
Summary: The emergence of COVID-19 presents a major challenge to the world, with an urgent need for effective intervention strategies. Bioactive compounds in bee products may offer a promising approach to fight against the virus, but further research is required.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Veterinary Sciences
Ahmed Gareh, Amira A. Saleh, Samar M. Moustafa, Amin Tahoun, Roua S. Baty, Refaat M. A. Khalifa, Ahmed K. Dyab, Doaa A. Yones, Mohsen Arafa, Amer R. Abdelaziz, Fatma A. El-Gohary, Ehab Kotb Elmahallawy
Summary: Cystic echinococcosis, a major parasitic zoonosis associated with economic losses, was investigated in slaughtered camels and cattle in Assiut Governorate, Egypt. The parasite was found exclusively in camel lungs and mainly in cattle lungs, with different fertility rates in the two hosts. Factors like age and sex were associated with the occurrence of the disease in cattle, but not in camels. The study confirmed the presence of the parasite in both hosts, indicating their potential role in the transmission cycle.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Ehab Kotb Elmahallawy, Hesham A. Sadek, Dina Aboelsoued, Maha A. Aloraini, Abdulsalam A. M. Alkhaldi, Salma M. Abdel-Rahman, Hanna Y. Bakir, Mohsen I. Arafa, Ehssan Ahmed Hassan, Elzahara Elbaz, Eman A. A. Hassanen, Fatma A. El-Gohary, Ahmed Gareh
Summary: This study investigated the occurrence of Cryptosporidium in cattle and buffalo from Upper Egypt. Results showed a high infection rate in both animals, with different factors such as age, sex, and season influencing the likelihood of infection.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Ahmed Gareh, Dalia Hassan, Asmaa Essa, Saber Kotb, Mohammed Karmi, Abou El-Hamd H. Mohamed, Abeer Mousa Alkhaibari, Elzahara Elbaz, Nagwa M. Elhawary, Eman A. A. Hassanen, Maha S. Lokman, Fatma A. El-Gohary, Ehab Kotb Elmahallawy
Summary: This study investigated the acaricidal activity of neem seed extracts on camel ticks and found that the hexane extract was the most effective in killing the ticks. It can be used economically for controlling H. dromedarii ticks.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Heba A. Ahmed, Rasha M. El Bayomi, Rehab Hamed, Rasha A. Mohsen, Fatma A. El-Gohary, Ahmed A. Hefny, Eman Elkhawaga, Hala M. N. Tolba
Summary: This study determined the prevalence and toxin types of antibiotic-resistant C. perfringens in different sources. The inhibitory effects of AgNPs on biofilm formation ability were investigated, and the genetic relatedness of isolates from various sources was determined using RAPD-PCR. The findings showed the high prevalence of multidrug-resistant C. perfringens and the potential of AgNPs in inhibiting biofilm formation.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Ahmed Gareh, Mahmoud Soliman, Amira A. Saleh, Fatma A. El-Gohary, Heba M. M. El-Sherbiny, Ragab H. Mohamed, Ehab Kotb Elmahallawy
VETERINARY SCIENCES
(2020)
Article
Agriculture, Dairy & Animal Science
Jesper Tessin, Judith Rohde, Arne Jung, Nicole Kemper, Jochen Schulz
Summary: In this study, samples from poultry house environment and culled birds were collected, and E. cecorum was successfully isolated. The principle of isolating the pathogen by streaking on a chromogenic agar may motivate researchers to investigate transmission routes and optimize biosecurity measures.
Article
Agriculture, Dairy & Animal Science
Junqin Zhang, Zimin Xie, Yanlin Pan, Zuoxin Chen, Yunzhen Huang, Linlin Li, Jiawen Dong, Yong Xiang, Qi Zhai, Minhua Sun, Xingying Li, Shujian Huang, Ming Liao
Summary: In recent years, there has been an increase in the occurrence of fowl adenovirus 2 (FAdV-2) in China, posing a significant threat to the poultry industry. This study investigated the epidemiology, phylogenetic relationship, genomic characteristics, and pathogenicity of FAdV-2. The results showed that FAdV-2 was the most commonly detected serotype among various FAdV serotypes. Genetic analysis revealed that these FAdV-2 strains formed a distinct branch within the FAdV-D group and had a high nucleotide similarity with reference strains. Recombination analysis identified recombination events between FAdV-2 and FAdV-11 in some strains. Pathogenicity experiments demonstrated that a recombinant FAdV-2 strain caused a high mortality rate and more severe disease in chickens. These findings contribute to our understanding of FAdV-2 and provide a basis for vaccine development.
Article
Agriculture, Dairy & Animal Science
Ebtsam Iraqi, Ali Abdel Hady, Nadia Elsayed, Hanaa Khalil, Amina El-Saadany, Karim El-Sabrout
Summary: Thermal manipulation during egg incubation, particularly at 12 to 18 days, has positive effects on embryonic development, hatching characteristics, chick quality, as well as posthatch thermotolerance and performance.
Article
Agriculture, Dairy & Animal Science
Yong Liu, Mingxin Song, He Bai, Chunhua Wang, Fei Wang, Qi Yuan
Summary: Dietary curcumin supplementation can significantly improve egg quality of quails in the late laying period, primarily by improving lipid metabolism and selectively regulating the intestinal microbial community.
Article
Agriculture, Dairy & Animal Science
Lei Zhang, Zhixuan Song, Peiyao Li, Xingju Song, Xinming Tang, Dandan Hu
Summary: This study evaluated the vaccine potential of recombinant proteins for avian coccidiosis caused by Eimeria. The results showed that the rEten5-B protein can trigger immune protection against E. tenella and may be a potential and effective subunit vaccine for the control of coccidiosis in poultry.
Article
Agriculture, Dairy & Animal Science
Milan Kumar Sharma, Amit Kumar Singh, Doyun Goo, Venkata Sesha Reddy Choppa, Hanseo Ko, Hanyi Shi, Woo Kyun Kim
Summary: The study investigates the effect of Eimeria infection on gastrointestinal physiology, growth performance, and egg production of Hy-Line W-36 laying hens during peak production. Results show that Eimeria infection negatively affects body weight, feed intake, immune response, and nutrient transporter gene expression. Egg production temporarily ceases in hens infected with high and medium-high dosages of Eimeria.
Article
Agriculture, Dairy & Animal Science
Elwy A. Ashour, Mahmoud Kamal, Hayman A. A. Altaie, Ayman A. Swelum, Gamaleldin M. Suliman, Guillermo Tellez-Isaias, Mohamed E. Abd El-Hack
Summary: This study evaluated the effects of CP and ME on the performance and nutritional value of Japanese quail lay chicken. The results showed that protein and energy levels significantly influenced all productive outcomes except feed conversion ratio. The combination of dietary energy and protein levels had a substantial effect on all egg quality trials evaluated. It is recommended to feed layer Japanese quail between the ages of 8 and 20 weeks during the summer with a dietary energy content of 2,900 kcal ME/kg and 20% CP.
Article
Agriculture, Dairy & Animal Science
J. E. Doornweerd, R. F. Veerkamp, B. de Klerk, M. van der Sluis, A. C. Bouwman, E. D. Ellen, G. Kootstra
Summary: Tracking group-housed individual broilers using video can provide valuable information on their health, welfare, and performance. This study conducted a comprehensive analysis on broiler tracking on video and potential tracking errors, highlighting the potential and challenges of phenotyping broiler locomotion. The results emphasized the importance of addressing tracking errors, potential algorithm improvements, and the need for an external animal identification system for efficient locomotion phenotyping.
Article
Agriculture, Dairy & Animal Science
Jiayu Wu, Yanan Wang, Yu An, Changyu Tian, Lingfeng Wang, Zuhong Liu, Desheng Qi
Summary: This study compared the gene expression differences in liver tissues of laying hens at different ages using RNA sequencing, and found that the transport and metabolism of amino acids changed during the growing period, which influenced the growth and development of the hens.
Article
Agriculture, Dairy & Animal Science
J. Raabe, G. Raveendran, W. Otten, K. Homeyer, T. Bartels
Summary: This study investigated the effects of different flicker frequencies on the behavior, performance, and stress response in male turkeys. The results showed that flicker frequencies of 165 Hz or higher had no detrimental effect on growth performance, injurious pecking, or endocrine stress response in the turkeys.
Article
Agriculture, Dairy & Animal Science
Adamu Mani Isa, Yanyan Sun, Yuanmei Wang, Yunlei Li, Jingwei Yuan, Aixin Ni, Hui Ma, Lei Shi, Hailai Hagos Tesfay, Yunhe Zong, Panlin Wang, Pingzhuang Ge, Jilan Chen
Summary: This study sequenced mRNAs and lncRNAs in the ovary stroma of specific chicken breeds and their reciprocal crossbreds to understand the molecular mechanism of heterosis for egg production. Results showed that genes with nonadditive expression were related to follicle growth and atresia as well as oxidative stress in the crossbred hens.
Article
Agriculture, Dairy & Animal Science
Zhepeng Wang, Qiu Chen, Yiwei Wang, Yulu Wang, Ruifang Liu
Summary: The study investigates the functional variants associated with chicken eggshell color in the SLCO1B3 gene by measuring the eggshell color of Lueyang black-boned chickens and analyzing the genetic data. It identifies 15 variants in SLCO1B3 that are significantly associated with eggshell color. However, the functional analysis shows that one specific variant, 1B3_SNP108, does not have a significant effect on promoter activity. This study highlights the importance of SLCO1B3 and its variants in breeding eggshell color.
Article
Agriculture, Dairy & Animal Science
P. Yin, Q. Tong, B. M. Li, W. C. Zheng, Y. Wang, H. Q. Peng, X. L. Xue, S. Q. Wei
Summary: This study investigated the welfare and health of laying hens in a multitier system. The results showed that the spatial distribution of hens changed with increasing age, and lower-ranking hens preferred to stay in lower tiers to avoid competition but still had access to resources. This improved the welfare and health of the hens near the end of the laying cycle.
Article
Agriculture, Dairy & Animal Science
Wenwen Liang, Siqiong Jie, Yiqin Wang, Xiaowei Wen, Yanling Xiong, Xiaoxi Lin, Zhengrong Lv
Summary: This study examined the effect of risk perceptions on preventive behavior among vendors in the live poultry wholesale market and the live poultry retail market in Guangdong Province using the health belief model and structural equation modeling. The results showed that perceived severity and perceived benefits positively influenced vendors' self-efficacy in the wholesale market, while perceived barriers negatively affected self-efficacy in both the wholesale and retail markets. Moreover, cues to action mediated the relationship between perceived severity, perceived benefits, and self-efficacy, and also mediated the effect of perceived susceptibility among wholesale market vendors.
Article
Agriculture, Dairy & Animal Science
Shreeya Sharma, Raveendra R. Kulkarni, Shayan Sharif, Hosni Hassan, Mohammadali Alizadeh, Scott Pratt, Khaled Abdelaziz
Summary: The study investigates the impact of exposing chick embryos to antigens via in ovo delivery of poultry-specific lactobacilli on the expression of genes associated with early bursal development and maturation. Results indicate that the supplementation of poultry-specific lactobacilli to chick embryos can accelerate the development and immunological maturation of the bursa of Fabricius.
