Article
Biochemistry & Molecular Biology
Ahmad Fayyazbakhsh, Marek Koutny, Alena Kalendova, Dagmar Sasinkova, Marketa Julinova, Marketa Kadleckova
Summary: This experimental research investigated the effects of different types of essential oils on the degradation of polyhydroxybutyrate (PHB). It was found that adding certain essential oils can reduce the biodegradation rate of PHB. SEM, DSC, and DMA were used to characterize the samples. The results showed that PHB blended with 3% eucalyptol, limonene, and thymol had biodegradation percentages of 66.4%, 73.3%, and 76.9%, respectively, after 226 days. Additionally, these additives exhibited good mechanical properties, with eucalyptol showing the highest resistance level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Energy & Fuels
Alicja Stasczak, Hanna Langer-Maciol, Karolina Widzisz, Wiktoria Sliwinska, Kinga Lucinska, Przemyslaw Wencel, Barbara Strozik, Mariusz Frackiewicz, Piotr Skupin, Dariusz Choinski, Sebastian Student
Summary: To date, the production and disposal of plastics have caused high pollution and carbon release. Bioplastics, especially polyhydroxybutyrate (PHB) polymer, are considered as an environmentally friendly alternative. This study investigates efficient strategies for bioplastic production using a mixture of oil and peptone as the substrate. Pseudomonas fluorescens bacteria are used for PHB accumulation in batch and continuous-flow bioreactors. Microscopic observations and laboratory essays confirm the presence of PHB and other important parameters, enabling the determination of optimal feeding strategies.
Review
Polymer Science
Maria de los Angeles Martinez Martinez, Lucia Soto Urzua, Yovani Aguilar Carrillo, Mirian Becerril Ramirez, Luis Javier Martinez Morales
Summary: In this review, the PHB production by A. brasilense is analyzed, including the carbon and nitrogen sources used, the synthesis and metabolic pathways of PHB, the role of genes involved in PHB metabolism, and the applications of PHB in the physiology of Azospirillum and biomedical and commercial areas.
Article
Biochemistry & Molecular Biology
Sun Mi Lee, Hong-Ju Lee, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Sion Ham, Jong-Min Jeon, Jeong-Jun Yoon, Shashi Kant Bhatia, Ranjit Gurav, Eun Yeol Lee, Yung-Hun Yang
Summary: This study screened and characterized ten xylose-utilizing PHB producers isolated from South Korean marine environments. Among these isolates, a newly identified strain Bacillus sp. SM01 produced the highest amount of PHB using xylose. Co-culturing Bacillus sp. SM01 with Cupriavidus necator NCIMB 11599 resulted in increased PHB production and overcome the limitation of sugar consumption associated with Bacillus sp. SM01 and C. necator.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Fatimah S. Al-Khattaf, Mysoon M. Al-Ansari, Murali Kannan Maruthamuthu, L. Dyona, Paul Agastian
Summary: The study aimed to degrade poly-beta-hydroxybutyrate (P(3HB)) in the sequencing batch biofilm reactor (SBBR) using biocatalyst, with enrichment method successfully isolating P(3HB) degrading bacteria such as Pseudomonas aeruginosa KS10 and Nitrobacter vulgaris SW1. Results showed that these bacteria were effective in degrading P(3HB), reducing environmental pollution associated with petroleum-based polymers.
ENVIRONMENTAL RESEARCH
(2022)
Article
Engineering, Environmental
Jorge Luis Meraz, Anthony J. Abel, Douglas S. Clark, Craig S. Criddle
Summary: Bioconversion of methane into PHB offers a promising solution for methane management. This study develops a dynamic model that accurately predicts the growth and PHB accumulation of Type II methanotrophs. The model explores the impact of various factors on productivity and energy efficiency and identifies operational windows for optimizing PHB production.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
P. R. Yashavanth, Meenakshi Das, Soumen K. Maiti
Summary: Plastic, while crucial in various sectors, poses severe environmental hazards due to its non-biodegradable nature. Eco-friendly bioplastics like PHB, produced from bacteria like cyanobacteria, show potential to replace conventional plastics. Challenges in industrial-scale PHB production still exist despite advancements in upstream and downstream processing strategies.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Polymer Science
Maria Fogasova, Silvestr Figalla, Lucia Danisova, Elena Medlenova, Slavka Hlavacikova, Zuzana Vanovcanova, Leona Omanikova, Andrej Baco, Vojtech Horvath, Maria Mikolajova, Jozef Feranc, Jan Bockaj, Roderik Plavec, Pavol Alexy, Martina Repiska, Radek Prikryl, Sona Kontarova, Anna Barekova, Martina Slavikova, Marek Koutny, Ahmad Fayyazbakhsh, Marketa Kadleckova
Summary: In order to make bioplastics more widely applicable, ready-to-use plastic material formulations with tailored properties should be available, ideally being home-compostable. This article presents materials based on PLA and PHB blends, which are intended for various applications and are fully biodegradable.
Article
Biochemistry & Molecular Biology
Sun Mi Lee, Hong-Ju Lee, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Sion Ham, Hun-Suk Song, Shashi Kant Bhatia, Ranjit Gurav, Jong-Min Jeon, Jeong-Jun Yoon, Kwon-Young Choi, Jae-Seok Kim, Sang Ho Lee, Yung-Hun Yang
Summary: The engineered Shewanella marisflavi BBL25 can produce PHB by inserting polyhydroxyalkanoates synthesis genes from Ralstonia eutropha H16. High PHB production was achieved using barley straw hydrolysates as a carbon source. In addition, simultaneous production of PHB and electricity was demonstrated using the microbial fuel cell system.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Andreas Eich, Miriam Weber, Christian Lott
Summary: The seafloor is a major sink for plastic debris, including biodegradable polymers. Sediment grain size influences the degradation rate of biodegradable plastics, with finer sediments promoting faster degradation due to higher bacterial abundance. Different sediments from various beaches can impact the disintegration rates of biodegradable plastics differently.
Article
Biochemistry & Molecular Biology
Maria Swiontek Brzezinka, Agnieszka Richert, Agnieszka Kalwasinska, Joanna Swiatczak, Edyta Deja-Sikora, Maciej Walczak, Marta Michalska-Sionkowska, Katarzyna Piekarska, Beata Kaczmarek-Szczepanska
Summary: The research aimed to isolate biofilm-forming bacteria capable of degrading PHB with PHMG derivatives. Different derivatives showed varying effects on biodegradation and biofilm formation in water and compost, with PHMG polyethylene wax granulate significantly inhibiting BOD at high concentrations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Piotr Jablonski, Dariush Nikjoo, Johan Warna, Knut Irgum, Jyri-Pekka Mikkola, Santosh Govind Khokarale
Summary: This report introduces a sustainable, metal-free, and single solvent-based reaction approach for the selective depolymerization of biopolymers to industrially valuable chemicals. The study investigates the influence of different reaction parameters on the conversion yield and efficiency.
Article
Biotechnology & Applied Microbiology
Meghna R. Malik, Nii Patterson, Nirmala Sharma, Jihong Tang, Claire Burkitt, Yuanyuan Ji, Matthew Martino, Andrew Hertig, Dirk Schweitzer, Oliver Peoples, Kristi D. Snell
Summary: Plant-based production of polyhydroxyalkanoates (PHAs) and seed oil can provide a sustainable source of feedstocks for renewable fuels and plastics. This study successfully engineered Camelina sativa to produce PHA poly[(R)-3-hydroxybutyrate] (PHB) in the seed cytosol using a bacterial PHB biosynthetic pathway. By localizing the key enzymes to the cytosol and endoplasmic reticulum, stable polymer production was achieved with PHB levels reaching 10.2% of seed weight. This research represents a significant advancement towards commercial use of plant-based PHAs, connecting large-scale agricultural production with the plastics and renewable fuels markets.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Mohammad Mohammadalipour, Saeed Karbasi, Tayebeh Behzad, Zahra Mohammadalipour, Maryam Zamani
Summary: The choice of materials and preparation methods are crucial for the characteristics of scaffolds. In this study, cellulose nanofibers were used as nano-additives to prepare a polyhydroxybutyrate (PHB) based nanocomposite scaffold. The properties of the PHB/CNF scaffold were compared with pure PHB, and the results showed improvements in crystallinity, water contact angle, degradation rate, biomineralization, toughness factor, and surface roughness. Moreover, the PC nanocomposite demonstrated better cell viability and spreading, indicating its potential in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Seo A. Park, Kwon-Young Choi
Summary: Indigo derivatives incorporating Poly(3-hydroxybutyrate) (IDPs) were produced in a single recombinant Escherichia coli strain through a one-pot process. The IDPs were analyzed for various biological and physical properties, revealing that 5-OH IDP exhibited higher thermal stability, while 4-NO2 IDP showed the lowest resistance to biodegradation.
Article
Biochemical Research Methods
Shaobin Zhang, Yiqing Wang, Xin Jiang, Zhanyong Wang
Summary: It was found in this study that the soluble expression of a pea actin isoform (PEAc1) in E. coli was facilitated by a GFP fusion tag. The addition of a GFP fusion tag improved the solubility and activity of PEAc1, making it more similar to muscle actin. This enhancement is likely due to the correct folding of PEAc1 mediated by GFP fusion.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Polymer Science
Ting Hu, Yujun Wang, Jingwei Lin, Zhanyong Wang
Summary: This study found that the enzymatic hydrolysis of poly(butylene succinate) (PBS), poly(hexamethylene succinate) (PHS), and poly(butylene succinate-co-hexamethylene succinate) (P(BS-co-HS)) copolymers is influenced by their crystallinity, thermal properties, and the ratio of BS to HS. By adjusting the BS/HS ratio, it is possible to prepare polyesters with different physical properties and degradability for various applications. During enzymatic hydrolysis, both the crystalline and amorphous regions of the polyesters are hydrolyzed, with some of the crystalline regions converting into amorphous regions. Notably, the enzymatic hydrolysis does not affect the crystal structure and thermal stability of the polyesters.
INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION
(2023)
Article
Materials Science, Multidisciplinary
Dawei Zhang, Chengling Yang, Hanwen Wang, Yukun Yan, Zhanyong Wang, Xiangrong Li, Yan Liang, Jinsong Zhang, Jun Xiao
Summary: In this study, highly crystalline conjugated polymer ODA-BPAH was prepared and found to exhibit superior photocatalytic performance. The results revealed that the crystallinity of conjugated polymers is related to their photocatalytic efficiency and the generation of reactive oxygen species. This study provides a new promising metal-free photocatalyst for water treatment.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Xiaodong Lin, Bergoi Ibarlucea, Tao Peng, Ren Shen, Peiyue Li, Pan Zhang
Summary: This study synthesized vancomycin-modified copper selenide nanoparticles that can be used for photothermal determination and hyperthermal inactivation of bacteria. The strategy provides a sensitive point-of-care testing method for bacterial determination and efficient bacterial inactivation.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Materials Science, Biomaterials
Binfeng Yin, Wenkai Yue, A. S. M. Muhtasim Fuad Sohan, Xinhua Wan, Teng Zhou, Liuyong Shi, Changcheng Qian, Xiaodong Lin
Summary: In this study, the mixing characteristics of hyperbolic micromixers were investigated, and the effects of different hyperbolic curvatures, Reynolds numbers, and channel widths on the mixing performance were explored. An optimal micromixer was integrated into a microfluidic chip to fabricate a desirable hyperbolic microfluidic chip (DHMC) for the sensitive determination of inflammation marker PCT. The DHMC achieved a low limit of detection (LOD) of 0.17 ng mL(-1) using a chemiluminescence signal, making it a promising real-time platform for early clinical diagnosis.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biophysics
Xiaodong Lin, Haotian Wu, Shiyu Zeng, Tao Peng, Pan Zhang, Xinhua Wan, Yihan Lang, Biao Zhang, Yanwei Jia, Ren Shen, Binfeng Yin
Summary: A lanthanide-based ratiometric fluorescent probe was designed and integrated into a self-designed microfluidic chip for the automated determination of DPA. The platform showed high selectivity and sensitivity, with a detection limit as low as 10.11 nM. The device could achieve rapid detection in only 1 minute and was used for point-of-care testing using a smartphone app, simplifying complex processes and reducing testing times.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Environmental Sciences
Yujun Wang, Ting Hu, Wanting Zhang, Jinwei Lin, Zhanyong Wang, Shuxia Lyu, Haibin Tong
Summary: A mesophilic bacterial strain, Bacillus safensis PLA1006, was isolated from landfill soil and tested for growth on polylactic acid (PLA) emulsion medium. The strain produced protease and lipase, and caused whitening and etching on PLA films. It also increased the hydrophilicity of PLA films surface. The hydrolysis products of PLA by the strain contained lactic acid monomers and oligomers.
Article
Polymer Science
Wen Lin, Yujin Zhao, Tingting Su, Zhanyong Wang
Summary: The degradation behavior of PBAT films by different concentrations of Fusarium solani cutinase (FsC) was investigated. It was found that FsC could degrade both the amorphous region and the crystalline region of PBAT films, and its degradation activity was concentration-dependent. High concentration of FsC resulted in significant degradation of PBAT films, while low concentration had less apparent effect. FsC concentration and degradation time did not significantly affect the molecular weight of degraded PBAT.
POLYMER DEGRADATION AND STABILITY
(2023)
Article
Chemistry, Analytical
Haotian Wu, Xiaodong Lin, Minyang Zhao, Xiao Zhou, Yaqing Liu
Summary: Great progress has been made in DNA computing for cancerous diagnosis, but errors in data transmission are inevitable and directly impact computational reliability, which has been neglected in DNA computing-based diagnosis. Here, a conceptual DNA logic circuit with error correction function for liver cancer diagnosis was constructed by integrating redundant modules to trace logic operation results and judge the correctness of the final result. Furthermore, photothermal treatment function was integrated in the DNA logic circuit for targeted therapy in time. This strategy significantly improves diagnosis accuracy and reliability, with instructive application in biomedical engineering and clinic diagnosis and therapy.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xinghui Yu, Yongqiang Ma, Siyuan Liu, Chunchun Qi, Weiqi Zhang, Wen Xiang, Zhaoxian Li, Kai Yang, Shaoxian Duan, Xinrao Du, Jian Yu, Yan Xie, Zicheng Wang, Wentao Jiang, Li Zhang, Xiaodong Lin
Summary: Post-operative pathogenic infections in liver transplantation pose a serious threat to human health. Researchers have developed a chemiluminescence-based method for the rapid and sensitive detection of Staphylococcus aureus. By integrating specific capture and separation techniques, this portable device, integrated with a smartphone, enables specific detection of S. aureus with high antibacterial efficiency. The method is simple to use and cost-effective, demonstrating great potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Binfeng Yin, Xinhua Wan, Wenkai Yue, Teng Zhou, Liuyong Shi, Songbai Wang, Xiaodong Lin
Summary: Abusive use of 13-agonists can harm human health and food safety. Conventional assays cannot meet the demand for automated, high throughput, and rapid quantitative analysis of multiple 13-agonists in real samples. A portable automated chip system (PACS) was developed in this study to provide simultaneous point-of-care testing of multiple 13-agonists in the field, simplifying complex manual methods, shortening assay times, and improving sensitivity. The PACS enables sensitive and rapid detection of multiple 13-agonists in farms or markets lacking advanced laboratory facilities.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Applied
Xiaodong Lin, Minyang Zhao, Tao Peng, Pan Zhang, Ren Shen, Yanwei Jia
Summary: Pathogenic bacteria can be a major threat to food safety and human health, making it crucial to develop a rapid, portable, and sensitive method for their detection and discrimination. Nanomaterials have emerged as desirable nanoprobes, offering extraordinary properties for optical signal-enabled detection and identification of bacteria. This review focuses on the recent application of nanomaterial-based optical biosensors for the detection and discrimination of pathogenic bacteria in food safety, with particular emphasis on noble metal nanomaterials, fluorescent nanomaterials, and point-of-care testing (POCT). It also discusses future trends in bacterial detection and discrimination, including the role of machine learning in intelligent rapid detection and accurate identification of bacteria.
Article
Chemistry, Analytical
Wenhui Li, Xuecheng Zhang, Haojie Zhang, Cheng Zhang, Yingjie Chen, Cong Li, Yonghong Hu, Xiaoping Yu, Biao Zhang, Xiaodong Lin
Summary: A novel colorimetric immunoassay was developed using Pt@Au nanozymes and catalytic signal probes for the simultaneous detection of DMP and DBP. The method showed high sensitivity and accuracy and can be applied for quantitative analysis of DMP and DBP in baijiu and plastic bottled drinks.
Article
Food Science & Technology
Yuan Fang, Shusen Ding, Weiran Li, Jingjing Zhang, Hui Sun, Xiaodong Lin
Summary: In this study, a dual-channel nanosensor was developed for the detection of Ag+ using synthesized nanomaterials with specific response to Ag+. The sensor combines fluorescence and colorimetric analysis to improve the sensitivity and accuracy of detection, and has shown satisfactory results in real samples.
Article
Chemistry, Applied
Biao Zhang, Jingyi Zhang, Yihan Lang, Zicheng Wang, Danfeng Cai, Xiaoping Yu, Xiaodong Lin
Summary: A novel dual-mode nanoplatform based on sea urchin-shaped Pt@Au NPs with high peroxidase-like activity is developed for sensitive detection of histamine. The colorimetric-based UV-vis nanoplatform allows for sensitive detection of histamine with a linear range of 0.5-100 ng/mL and a low limit of detection (LOD) of 0.3 ng/mL. Additionally, a smartphone-loaded color picker APP can intelligently detect histamine based on the R/B ratio in the color channels, achieving a detection range of 0.5-1000 ng/mL and a LOD as low as 0.15 ng/mL, significantly expanding the detection range. This easy-to-use and sensitive detection system shows great potential in ensuring the safety of protein-rich meat food.
