Article
Biochemistry & Molecular Biology
Mirjana Radomirovic, Nikola Gligorijevic, Dragana Stanic-Vucinic, Andreja Rajkovic, Tanja Cirkovic Velickovic
Summary: An ultrasensitive immuno-PCR method was developed for quantifying crustacean tropomyosin in foods. It showed high specificity and precision, with a quantification sensitivity 20-fold higher than ELISA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Zhi Li, Chuqin Yang, Wenying Lu, Zhaohui Chu, Jingwen Zhang, Ming Li, Qingjiang Wang
Summary: In this study, a magnetic immuno-PCR method based on barcode DNA was developed and evaluated for ultrasensitive detection of ultratrace aflatoxin B-1 (AFB(1)) in agricultural by-product samples. The method demonstrated significantly improved sensitivity compared to ELISA and showed reasonable specificity, precision, and accuracy. The reliability and applicability of the method were further confirmed by correlating the results with LC-MS/MS. The method significantly shortened the analytical procedure, testing time, and workload, indicating high-efficiency performance.
Article
Engineering, Biomedical
Yi-Chia Hsieh, Chih-Yu Lin, Hsin-Yao Lin, Chun-Ting Kuo, Shin-Yi Yin, Ying-Hua Hsu, Hsiu-Feng Yeh, Jane Wang, Dehui Wan
Summary: A painless and rapid biofluid analysis platform with ultrasensitive molecular recognition capability is developed using microneedle patches. The patches enable efficient extraction of skin interstitial fluid and maintain structural integrity. Analyte molecules can be recovered into a moist cellulose paper and functionalized for desired detection capacity. Nicotine is used as a model drug to demonstrate the painless monitoring of three human volunteers. This minimally invasive sensing system has potential applications in precision medicine and personal healthcare monitoring.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Margherita Passariello, Asami Yoshioka, Kota Takahashi, Shu-ichi Hashimoto, Rosa Rapuano Lembo, Lorenzo Manna, Koji Nakamura, Claudia De Lorenzo
Summary: Cancer immunotherapy has achieved significant progress by combining different antibodies targeting distinct immune checkpoints. Researchers have tested combinatorial treatments of immunomodulatory antibodies and found that some antibodies showed additive or synergistic effects. They have also constructed four novel bispecific tribodies that exhibited enhanced lymphocyte activation and cytotoxicity against tumor cells, while not causing significant cytotoxicity on human cardiomyocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Adilet Beishenaliev, Yean Leng Loke, Sook Jing Goh, Hui Nee Geo, Malar Mugila, Misni Misran, Lip Yong Chung, Lik Voon Kiew, Steve Roffler, Yin Yin Teo
Summary: Monospecific antibodies have limitations in delivering drugs to tumors with multiple epitopes, while bispecific antibodies provide a promising alternative by simultaneously targeting two distinct antigens or epitopes. This review discusses the roles of bispecific antibodies in enhancing the internalization and intracellular trafficking of drug-conjugated antibodies and facilitating the delivery of drug-encapsulating nanoconstructs.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Pharmacology & Pharmacy
Roya Ramezankhani, Roya Solhi, Yoke Chin Chai, Massoud Vosough, Catherine Verfaillie
Summary: Efficient prophylactic and therapeutic strategies for COVID-19 require precise knowledge of SARS-CoV-2 pathogenesis. Advanced platforms like organoids and microfluidic devices offer promising perspectives for disease modeling and drug screening.
DRUG DISCOVERY TODAY
(2022)
Article
Biochemistry & Molecular Biology
Tyler C. Beck, Kendra Springs, Jordan E. Morningstar, Catherine Mills, Andrew Stoddard, Lilong Guo, Kelsey Moore, Cortney Gensemer, Rachel Biggs, Taylor Petrucci, Jennie Kwon, Kristina Stayer, Natalie Koren, Jaclyn Dunne, Diana Fulmer, Ayesha Vohra, Le Mai, Sarah Dooley, Julianna Weninger, Yuri Peterson, Patrick Woster, Thomas A. Dix, Russell A. Norris
Summary: Cancer is the second leading cause of death in the United States, and despite improvements in treatment, new oncology drugs come with increased toxicity risk. Early-stage implementation of pharmacokinetic prediction tools is necessary for designing safer drugs.
Article
Gastroenterology & Hepatology
Quentin Tournier, Stephane Paul, Nicolas Williet, Anne-Emmanuelle Berger, Pauline Veyrard, Gilles Boschetti, Bertrand Le Roy, Martin Killian, Jean Marc Phelip, Bernard Flourie, Stephane Nancey, Xavier Roblin
Summary: This study aimed to investigate whether the early detection of anti-drug antibodies during the induction therapy with anti-TNF drugs could predict treatment discontinuation. The results showed that antibodies to ADA or IFX detected in week 2 were associated with treatment failure and could predict treatment discontinuation in IBD patients within 24 months.
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Economics
A. A. Chursin, I. N. Dubina, E. G. Carayannis, A. E. Tyulin, A. V. Yudin
Summary: The article discusses the formation and functioning of technology platforms as a tool for scientific and technological innovation development at the local and global levels of the economic space. It defines a technology platform as a special type of business model and mechanism for organizing the innovation process, based on a specific, relatively stable network. Additionally, it reveals the relationship between the growth of intellectual and innovative potential with the change of technological structures, and proposes economic and mathematical models for the coordinated development of technology platforms.
JOURNAL OF THE KNOWLEDGE ECONOMY
(2022)
Article
Chemistry, Multidisciplinary
Claudia Martins, Catarina Pacheco, Catarina Moreira-Barbosa, Angela Marques-Magalhaes, Sofia Dias, Marco Araujo, Maria J. Oliveira, Bruno Sarmento
Summary: This study generated human GBM heterotypic multicellular tumor microtissues (MCTMs) using high-throughput 3D modeling, which mimicked the spatial organization and biological features of GBM. The bioactivity of tumor-targeted nanoparticles and anti-cancer drugs were evaluated in the MCTMs, and it was found that the targeted nanoparticles exhibited higher cell uptake and anti-proliferative effect.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Pharmacology & Pharmacy
Raef Shams, Yoshihiro Ito, Hideyuki Miyatake
Summary: mTOR is a protein kinase involved in cell growth and proliferation, which can be assembled into mTORC1 and mTORC2 complexes with different functions. In cancer cells, mTOR is hyperactivated to adapt to stress conditions, leading to efforts in developing compounds to inhibit mTOR and disrupt cancer cell metabolism.
PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Immunology
Alessandra Vultaggio, Margherita Perlato, Francesca Nencini, Emanuele Vivarelli, Enrico Maggi, Andrea Matucci
Summary: Biologicals are commonly used for rheumatologic diseases, cancers, and chronic inflammatory conditions, but they may lead to the development of anti-drug antibodies (ADA), limiting their clinical usage due to safety concerns. Strategies such as concomitant immunosuppressive treatment and regular infusion regimens can help reduce the risk of immediate hypersensitivity reactions (HSRs) associated with ADA. Drug desensitization (DD) may be considered for patients needing reintroduction of the biological culprit, especially in cases of IgE-mediated HSRs, potentially inhibiting mast cell degranulation and cytokine production.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Wen Shi Lee, Arnold Reynaldi, Thakshila Amarasena, Miles P. Davenport, Matthew S. Parsons, Stephen J. Kent
Summary: The study found that repeated intravenous doses of PGT121 led to the induction of anti-PGT121 ADA, which in turn resulted in decreased suppression of SHIV viremia.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Morgan D. McSweeney, Limei Shen, Alexander C. DeWalle, Jordan B. Joiner, Elizabeth C. Ciociola, Dharmendra Raghuwanshi, Matthew S. Macauley, Samuel K. Lai
Summary: This study explored the effectiveness of prophylaxis with free PEG or tolerogenic PEGylated liposomes as a strategy to reduce the amount of APA induced by subsequently administered PEGylated liposomes. The results showed that a single administration of free PEG alone was capable of markedly reducing the APA response to PEG-liposomes for up to 2 months, which was comparable to, and often exceeded, interventions with different tolerogenic PEG-liposomes. These findings support further research on free PEG prophylaxis as a potential strategy to mitigate the APA response to sensitizing PEGylated therapeutics.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Guus A. M. S. van Dongen, Wissam Beaino, Albert D. Windhorst, Gerben J. C. Zwezerijnen, Daniela E. Oprea-Lager, N. Harry Hendrikse, Cornelis van Kuijk, Ronald Boellaard, Marc C. Huisman, Danielle J. Vugts
Summary: The identification of molecular drivers of disease and the rise of biotherapeutics have impacted clinical care, but also presented challenges. The application of Zr-89-immuno-PET in drug development shows promise in guiding the design, development, and application of biologic drugs, with increasing potential for understanding drug-target combinations in clinical studies.
JOURNAL OF NUCLEAR MEDICINE
(2021)