Article
Chemistry, Multidisciplinary
Raghu Pradeep Narayanan, Abhay Prasad, Alex Buchberger, Lei Zou, Julio Bernal-Chanchavac, Tara Macculloch, Nour Eddine Fahmi, Hao Yan, Fei Zhang, Matthew J. Webber, Nicholas Stephanopoulos
Summary: The combination of multiple orthogonal interactions enables hierarchical complexity in self-assembled nanoscale materials. This study demonstrates the efficient supramolecular polymerization of DNA origami nanostructures using a multivalent display of small molecule host-guest interactions, and exploits high-affinity CB[7]-adamantane interactions to enable responsive enzymatic actuation.
Article
Chemistry, Multidisciplinary
Xuejiao Yang, Bihan Wu, Jiong Zhou, Honglei Lu, Hongyue Zhang, Feihe Huang, Huaimin Wang
Summary: This study demonstrates that host-guest complexation can efficiently regulate enzymatic self-assembly, leading to controlled cancer cell death.
Article
Chemistry, Multidisciplinary
Jinrong Ma, Bin Cai, Shuai Zhang, Tengyue Jian, James J. De Yoreo, Chun-Long Chen, Francois Baneyx
Summary: This study reports a simple and modular approach to hierarchical hybrid synthesis by synthesizing peptoid nanosheets and solid-binding protein to construct stacked multilayered materials, which is expected to be applied in bioimaging and photocatalysis fields.
Article
Chemistry, Physical
Meiwen Cao, Zijin Zhang, Xiaoyang Zhang, Yu Wang, Jingjing Wu, Zhihong Liu, Li Sun, Dong Wang, Tongtao Yue, Yuchun Han, Yingxiong Wang, Yilin Wang, Ming Wang
Summary: Smart artificial viruses have been developed through co-assembly of peptides and DNA, achieving stimuli-responsiveness and efficient gene transfection in cancer cells. These viruses protect DNA from digestion and release genes efficiently through enzyme-triggered structure disassembly. They resemble natural viruses in terms of structure and function, making them promising gene vectors.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Physical
Yong-Hong Gao, Kuo Zhang, Meng-Ru Ding, Xue-Feng Gong, Hui Cao, Lei Wang
Summary: Binding-induced fibrillogenesis (BIF) peptides have potential applications in biomedicine as they can bind to specific triggers and self-assemble into nanofibrous structures in vivo. The hydrophobicity of the alkyl chains in BIF peptides plays a crucial role in their self-assembly behaviors. In this study, BIF peptides with different hydrophobic alkyl chains were designed to investigate the influence of hydrophobicity on their self-assembly behaviors. The results showed that BIF peptides with weak hydrophobicity did not form fibrous structures upon binding to Ca2+, but formed nanoparticles instead. On the other hand, BIF peptides with strong hydrophobicity initially self-assembled into nanoparticles, which further bound to Ca2+ and self-assembled into nanofibers with a specific conformation. Additionally, when incubated with HUVECs overexpressing integrins, BIF peptides with weak hydrophobicity were internalized as nanoparticles without undergoing the self-assembly process, while BIF peptides with strong hydrophobicity underwent the self-assembly process and attached to the cell surfaces. This study provides experimental evidence of the hydrophobicity effect on the self-assembly behavior of BIF peptides and guides the applications of BIF peptides with controllable self-assembly.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Risako Kobayashi, Hiroshi Inaba, Kazunori Matsuura
Summary: This study utilized self-assembling beta-annulus peptides to construct an artificial viral capsid model and analyzed the effects of molecular crowding environment on the formation process of viral capsids. The results showed that the apparent particle size and dissociation constant (K-d) of the assemblies decreased with increasing concentration of the molecular crowding agent, polyethylene glycol (PEG). This study represents the first successful in situ analysis of the self-assembling process of artificial viral capsids under molecular crowding conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Dingze Mang, Sona Rani Roy, Qizheng Zhang, Xunwu Hu, Ye Zhang
Summary: This study developed HS-instructed self-assembling peptides to inhibit cancer cell migration by hindering the binding of HSPG with HBEGF, leading to the suppression of FAK and ERK activation. This approach shows promise as a synthetic therapeutic strategy for targeted cancer migration inhibition.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Shanshan Zhang, Sajid Asghar, Chenqi Zhu, Junxiu Ye, Ling Lin, Liu Xu, Ziyi Hu, Zhipeng Chen, Feng Shao, Yanyu Xiao
Summary: This study presents a multi-functional delivery system (APND-3) designed to address the issues of poor drug delivery to the brain in Alzheimer's disease (AD) treatment. The system, based on a novel peptide (MOP) with self-assembling properties, significantly enhances the delivery of MOP to the brain, reducing A beta deposition and improving neurological outcomes in AD model mice.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Milad Zangiabadi, Avijit Ghosh, Yan Zhao
Summary: The aggregation of Aβ peptides in Alzheimer's disease involves complex interactions between hydrophobic and polar residues. Molecularly imprinted nanoparticle (MINP) receptors strongly bind specific segments of Aβ(40), inhibiting the aggregation of monomeric A040 through binding residues 21-30 near the loop region. Residues 11-20, including the internal 0 strand closer to the N-terminal, represent the best target for disaggregating already formed aggregates during the polymerization phase. Binding residues 1-10 have the greatest effect on disaggregation in the saturation phase.
Article
Food Science & Technology
Miaochuan Lan, Yu Fu, Hongjie Dai, Liang Ma, Yong Yu, Hankun Zhu, Hongxia Wang, Yuhao Zhang
Summary: This study enzymatically prepared rapeseed peptides as a nanocarrier to encapsulate hydrophobic beta-carotene, optimizing the encapsulation conditions for efficiency. The optimal parameters included enzymatic hydrolysis for 60 minutes, encapsulation time for 3 hours, and a ratio of beta-carotene to peptides of 3/5, resulting in over 80% encapsulation efficiency and 0.55 mg/mg drug loading amount. The encapsulation structure was characterized to confirm successful encapsulation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Sara La Manna, Valentina Roviello, Vittoria Monaco, James A. Platts, Maria Monti, Elisabetta Gabano, Mauro Ravera, Daniela Marasco
Summary: This study investigated the ability of four Pt(II) complexes to modulate the self-aggregation processes of amyloidogenic models and found that phenanthriplatin had the most efficient anti-amyloid aggregation effect. Molecular dynamic simulations revealed the mechanism of action of phenanthriplatin.
DALTON TRANSACTIONS
(2023)
Article
Medicine, Research & Experimental
Jingjing Wang, Xiaoqiang Zheng, Xiao Fu, Aimin Jiang, Yu Yao, Wangxiao He
Summary: This article introduces a new method for the treatment of metastatic melanoma by using a bifunctional supramolecular peptide HBBplus@CA, which simultaneously inhibits both the Wnt/β-catenin and MAPK/ERK signaling pathways. It effectively suppresses melanoma cell proliferation, migration, and invasion, and inhibits lung metastasis and prolongs survival in a mouse model.
Article
Engineering, Biomedical
Ziv Azoulay, Polina Aibinder, Ayala Gancz, Jacob Moran-Gilad, Shiri Navon-Venezia, Hanna Rapaport
Summary: FKF, a tripeptide with potential antimicrobial activity, forms a pure peptide hydrogel phase made of natural amino acids. The hydrogel exhibits bactericidal activity against E. coli and P. aeruginosa, and reduces P. aeruginosa contamination in a rat skin lesion model.
ACTA BIOMATERIALIA
(2021)
Article
Biochemical Research Methods
Ville Tahtinen, Vijay Gulumkar, Sajal K. Maity, Ann-Mari Yliperttula, Saara Siekkinen, Toni Laine, Ekaterina Lisitsyna, Iida Haapalehto, Tapani Viitala, Elina Vuorimaa-Laukkanen, Marjo Yliperttula, Pasi Virta
Summary: Glyco-decorated spherical nucleic acids (SNAs) are attractive delivery vehicles that can hide the unfavorable distribution properties of loaded oligonucleotides. In this study, glyco-AONARV7 conjugates were successfully synthesized and used to form glyco-decorated SNAs. Cellular uptake experiments demonstrated that these glyco-decorated SNAs efficiently entered prostate cancer cells and showed marked variation in intracellular distribution.
BIOCONJUGATE CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Shuang Wang, Xiaolin Xie, Zhi Chen, Ningning Ma, Xue Zhang, Kai Li, Chao Teng, Yonggang Ke, Ye Tian
Summary: The DNA-grafted nanoparticle serves as a programmable atom equivalent for assembling three-dimensional superlattice with novel properties, allowing for the exploration of assembly strategies and mechanisms, as well as future applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kazunori Matsuura, Seiya Fujita
Summary: The study constructed an artificial viral capsid containing azobenzene, which could self-assemble into capsids of different sizes and encapsulate and release fluorescently labeled dextrans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Risako Kobayashi, Hiroshi Inaba, Kazunori Matsuura
Summary: This study utilized self-assembling beta-annulus peptides to construct an artificial viral capsid model and analyzed the effects of molecular crowding environment on the formation process of viral capsids. The results showed that the apparent particle size and dissociation constant (K-d) of the assemblies decreased with increasing concentration of the molecular crowding agent, polyethylene glycol (PEG). This study represents the first successful in situ analysis of the self-assembling process of artificial viral capsids under molecular crowding conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Hiroshi Inaba, Kazunori Matsuura
Summary: Researchers have discovered various proteins inside microtubules that can modulate their properties and functions, with Tau-derived peptides encapsulating nanomaterials showing new potential for artificial modulation of microtubules.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2021)
Article
Biochemical Research Methods
Kentarou Sakamoto, Hiroto Furukawa, Jan Vincent V. Arafiles, Miki Imanishi, Kazunori Matsuura, Shiroh Futaki
Summary: In this study, nanocarriers were used to deliver functional proteins to cell interiors without modifying the proteins. A protein nanocage was formed via self-assembly and was able to encapsulate cargo proteins inside the cell. The nanocage had a flexible design for surface and interior modifications. Successful intracellular delivery of cargo proteins and targeting of cytosolic proteins were achieved.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Hiroshi Inaba, Arif Md Rashedul Kabir, Akira Kakugo, Kazuki Sada, Kazunori Matsuura
Summary: In this study, gold nanoparticles were encapsulated in microtubules by conjugating a Tau-derived peptide. The rigidity and stability of the microtubules were found to increase using citrate-capped AuNPs, providing insights into the modulation of microtubules by nano-sized materials.
Article
Biochemistry & Molecular Biology
Hiroto Furukawa, Hiroshi Inaba, Yoshihiro Sasaki, Kazunari Akiyoshi, Kazunori Matsuura
Summary: This study demonstrated the embedding of membrane protein Connexin-43 (Cx43) onto an enveloped artificial viral capsid using a cell-free expression system. The Cx43 expression on the artificial viral capsid was confirmed by western blot analysis. Fluorescence correlation spectroscopy and transmission electron microscopy showed unique spherical structures in the Cx43-embedded enveloped viral replica.
RSC CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Sayuri L. Higashi, Ayaka Isogami, Junko Takahashi, Aya Shibata, Koichiro M. Hirosawa, Kenichi G. N. Suzuki, Shunsuke Sawada, Shinya Tsukiji, Kazunori Matsuura, Masato Ikeda
Summary: In this study, a new reduction-cleavable spacer (RCS) based on a nitrobenzene scaffold was designed and synthesized for constructing reduction-responsive oligonucleotides using standard phosphoramidite chemistry. Furthermore, it was demonstrated that the introduction of the RCS in the middle of an oligonucleotide (30 nt) allowed for the construction of a self-assembled microsphere capable of exhibiting a reduction-responsive disassembly.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Yingbing Liang, Hiroto Furukawa, Kentarou Sakamoto, Hiroshi Inaba, Kazunori Matsuura
Summary: In this study, we successfully generated RNase S-decorated artificial viral capsids with a size of 70-170 nm by self-assembly of the beta-annulus-S-peptide followed by reconstitution with S-protein at neutral pH. These capsids showed efficient uptake by HepG2 cells and exhibited higher RNA degradation activity compared to RNase S alone. Furthermore, cell viability assays demonstrated that RNase S-decorated capsids have potent anticancer activity comparable to standard anticancer drugs.
Article
Chemistry, Multidisciplinary
Yoshiyuki Manabe, Masato Tsutsui, Kohtaro Hirao, Risako Kobayashi, Hiroshi Inaba, Kazunori Matsuura, Daisuke Yoshidome, Kazuya Kabayama, Koichi Fukase
Summary: In this study, we synthesized B-antigen-displaying dendrimers of different sizes and evaluated their affinity to IgM antibody. Surprisingly, the smallest dendrimer exhibited multivalency and had similar or higher affinity compared to larger dendrimers. This suggests that the statistical rebinding model significantly contributes to the multivalency of glycodendrimers. Therefore, high-density glycan presentation to enhance statistical rebinding should be considered in addition to the ability to chelate multiple binding sites in the design of glycodendrimers.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Kosuke Hori, Shinichi Higashida, Tomohiro Osaki, Tsuyoshi Kawano, Hiroshi Inaba, Kazunori Matsuura, Takashi Iwasaki
Summary: This study demonstrates the efficacy of polyhistidine peptide-modified AuNPs as a drug delivery system for photothermal therapy against lung squamous cancer cells. The peptide-modified AuNPs were efficiently internalized into the cancer cells and localized to the lysosomes. Under laser irradiation, the AuNPs inhibited cell proliferation through increased expression of apoptosis-related and heat stress-related genes.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Hiroshi Inaba, Yurina Sueki, Muneyoshi Ichikawa, Arif Md. Rashedul Kabir, Takashi Iwasaki, Hideki Shigematsu, Akira Kakugo, Kazuki Sada, Tomoya Tsukazaki, Kazunori Matsuura
Summary: In this study, we designed and synthesized TP-AG protein to construct superstructures of microtubules by controlling the binding sites. The binding of TP-AG to the inside promoted microtubule formation and generated rigid and stable microtubules, while the binding to the outside induced various microtubule superstructures. This method provides a new approach for understanding the functions of microtubules and constructing microtubule-based nanomaterials.
Article
Biochemistry & Molecular Biology
Hiroshi Inaba, Minamo Sakaguchi, Soei Watari, Shigesaburo Ogawa, Arif Md. Rashedul Kabir, Akira Kakugo, Kazuki Sada, Kazunori Matsuura
Summary: The research developed a Tau-derived peptide as a binding unit and conjugated it with a reversible spiropyran to stabilize microtubules. It was found that the peptide with reversible spiropyran induced polymerization and stabilization of microtubules, and reversible formation of microtubule/tubulin aggregates was achieved through UV and visible light irradiation.
Article
Chemistry, Multidisciplinary
Soei Watari, Hiroshi Inaba, Tomonori Tamura, Arif Md Rashedul Kabir, Akira Kakugo, Kazuki Sada, Itaru Hamachi, Kazunori Matsuura
Summary: To manipulate cells, microtubules (MTs) can be stabilized using light-induced reactions. This study conjugated a photo-reactive diazirine group with a Tau-derived peptide to bind to the inside of MTs. Irradiation with ultraviolet (UV) light formed a covalent bond between the peptide and MTs, resulting in significant stabilization but also showing toxicity.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Kazunori Matsuura, Koichi Hisamoto, Tomoya Tanaka, Ryota Sakamoto, Mizuki Okazaki, Hiroshi Inaba
Summary: A simple fluorescent probe based on a dansyl triarginine peptide was developed for turn-on imaging of gangliosides on liposome membranes, showing high selectivity for monosialyl gangliosides. This probe could provide a novel molecular tool for chemical biology by enhancing the fluorescence intensity upon binding to monosialyl ganglioside-containing giant liposomes.
Article
Chemistry, Multidisciplinary
Kazunori Matsuura, Koichi Hisamoto, Tomoya Tanaka, Ryota Sakamoto, Mizuki Okazaki, Hiroshi Inaba
Summary: A fluorescent probe based on a dansyl triarginine peptide was developed for turn-on imaging of gangliosides, selectively enhancing fluorescence intensity upon binding to monosialyl gangliosides. This simple peptide probe offers a novel molecular tool for chemical biology research.
ACS ORGANIC & INORGANIC AU
(2021)
