期刊
FRONTIERS IN CELLULAR NEUROSCIENCE
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fncel.2015.00102
关键词
hemichannels; connexins; pannexins; stress; hippocampus; glia; neuron
资金
- FONDECYT [11121133, 1130724]
- Committe for Aid and Education in Neurochemistry from International Society for Neurochemistry
- NUCLEO UNAB [DI-603-14/N]
- CORFO [14IDL2-30195]
- ICM-ECONOMIA [P09-022-F]
Stress affects brain areas involved in learning and emotional responses, which may contribute in the development of cognitive deficits associated with major depression. These effects have been linked to glial cell activation, glutamate release and changes in neuronal plasticity and survival including atrophy of hippocampal apical dendrites, loss of synapses and neuronal death. Under neuro-inflammatory conditions, we recently unveiled a sequential activation of glial cells that release ATP and glutamate via hemichannels inducing neuronal death due to activation of neuronal NMDA/P2X7 receptors and pannexinl hemichannels. In the present work, we studied if stress induced glia activation is associated to changes in hemichannel activity. To this end, we compared hemichannel activity of brain cells after acute or chronic restraint stress in mice. Dye uptake experiments in hippocampal slices revealed that acute stress induces opening of both Cx43 and Panxl hemichannels in astrocytes, which were further increased by chronic stress; whereas enhanced Panxl hemichannel activity was detected in microglia and neurons after acute/chronic and chronic stress, respectively. Moreover, inhibition of NMDA/P2X7 receptors reduced the chronic stress induced hemichannel opening, whereas blockade of Cx43 and Panxl hemichannels fully reduced ATP and glutamate release in hippocampal slices from stressed mice. Thus, we propose that gliotransmitter release through hemichannels may participate in the pathogenesis of stress associated psychiatric disorders and possibly depression.
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