Review
Oncology
Yibo Guo, Juan Luo, Hailin Zou, Chenxin Liu, Liang Deng, Peng Li
Summary: This article provides an overview of the core components and upstream regulators of Hippo-YAP/TAZ signaling in mammals, and systematically summarizes the identified transcription factors or partners responsible for the downstream transcriptional output of YAP/TAZ in various cancers.
Article
Medicine, Research & Experimental
Ryan D. Watkins, EeeLN H. Buckarma, Jennifer L. Tomlinson, Chantal E. McCabe, Jennifer A. Yonkus, Nathan W. Werneburg, Rachel L. Bayer, Patrick P. Starlinger, Keith D. Robertson, Chen Wang, Gregory J. Gores, Rory L. Smoot
Summary: This study found that the use of a selective SHP1/SHP2 inhibitor, NSC-87877, can accelerate hepatocyte proliferation, improve liver regeneration, and reduce injury after hepatectomy. It also demonstrated the dependence of these effects on YAP, highlighting the role of these molecules in the enhanced regenerative response. Furthermore, NSC-87877 treatment was beneficial in a murine model of nonalcoholic steatohepatitis.
Article
Multidisciplinary Sciences
Petros X. E. Mouratidis, Marcia Costa, Ian Rivens, Elizabeth E. Repasky, Gail ter Haar
Summary: The study showed that pulsed high-intensity focused ultrasound (pHIFU) combined with immune checkpoint inhibitors (ICI) has the potential to enhance survival in pancreatic cancer therapy based on increased infiltration of CD8(+) T cells and extended survival in treated subjects compared to controls.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2021)
Article
Biochemistry & Molecular Biology
Yuexian Li, Cheng Sun, Yonggang Tan, Heying Zhang, Yuchao Li, Huawei Zou
Summary: The study identified high expression of ITGB1 in radioresistant non-small cell lung cancer cells. ITGB1 was found to induce radioresistance by affecting DNA repair and YAP1-induced epithelial-mesenchymal transition. Inhibiting ITGB1 may be a potential strategy to overcome radioresistance in NSCLC cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xueke Li, Yi Zhong, Wuqi Zhou, Yishu Song, Wenqu Li, Qiaofeng Jin, Tang Gao, Li Zhang, Mingxing Xie
Summary: LIPUS stimulation on BMSCs increases the secretion of EVs, which possess stronger anti-inflammatory function. RNA-seq analysis reveals upregulation of miR-328-5p and miR-487b-3p in LIPUS-EVs, potentially suppressing the MAPK signaling pathway to enhance the anti-inflammatory effects.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Cell & Tissue Engineering
Robi A. R. Bolli, Chandrashekhar Dasari, Asma Arshia, Dinesh Devadoss, Yiru Guo, Usman Ashraf, Qianhong Li
Summary: Culturing cardiac mesenchymal cells (CMCs) at 5% O-2 tension improves cell morphology, decreases cell size, increases cell number, and enhances cell function, which may enhance the regenerative properties of the cells. Furthermore, CMCs cultured at 5% O-2 display increased resistance to hypoxic stress and may have better survival rates after transplantation into low oxygen regions of the heart. This suggests a new method for culturing CMCs and other progenitor cells to improve their therapeutic potential.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Orthopedics
Peng Xia, Qi Wang, Jiulong Song, Xiaoju Wang, Xinwei Wang, Qiang Lin, Kai Cheng, Anliang Chen, Xueping Li
Summary: The study showed that LIPUS can promote MSC exosome release by activating autophagy, enhancing the positive effects of MSCs on OA cartilage repair. In vivo results demonstrated that LIPUS significantly improved the therapeutic efficacy of MSCs in OA cartilage repair, possibly through increasing autophagy-mediated exosome release.
Article
Physiology
Wanming Wu, Renfeng Huang, Linyang Ou, Ruiwen Lei
Summary: This study reveals that exosomes secreted by bone marrow mesenchymal stem cells (BM-MSCs) can promote the proliferation and migration of breast cancer cells. The Hippo signaling components YAP and TAZ are found to be involved in this process.
CHINESE JOURNAL OF PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Gianluca Iori, Johannes Schneider, Andreas Reisinger, Frans Heyer, Laura Peralta, Caroline Wyers, Claus C. Glueer, J. P. van den Bergh, Dieter Pahr, Kay Raum
Article
Engineering, Biomedical
Gianluca Iori, Laura Peralta, Andreas Reisinger, Frans Heyer, Caroline Wyers, Joop van den Bergh, Dieter Pahr, Kay Raum
MEDICAL ENGINEERING & PHYSICS
(2020)
Article
Endocrinology & Metabolism
Adalbert Raimann, Sarah N. Mehany, Patricia Feil, Michael Weber, Peter Pietschmann, Andrea Boni-Mikats, Radka Klepochova, Martin Krssak, Gabriele Haeusler, Johannes Schneider, Janina M. Patsch, Kay Raum
FRONTIERS IN ENDOCRINOLOGY
(2020)
Article
Acoustics
Laura Peralta, Juan Deyo Maeztu Redin, Fan Fan, Xiran Cai, Pascal Laugier, Johannes Schneider, Kay Rauw, Quentin Grimal
Summary: The study demonstrates a significant association between ultrasonic velocities in cortical bone and porosity as well as compression strength, suggesting the potential of using velocity measurements for diagnosing bone fragility in vivo.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2021)
Article
Acoustics
Gianluca Iori, Juan Du, Janos Hackenbeck, Vantte Kilappa, Kay Raum
Summary: Multichannel pulse-echo ultrasound combined with spectral backscatter analysis offers quantitative information on cortical bone microstructural properties. The study demonstrates the potential of ultrasound in predicting pore structure and material properties in cortical bone, which could have a significant impact on preventing osteoporotic fractures.
IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
(2021)
Article
Acoustics
Theresa H. Lye, Omar Gachouch, Lisa Renner, Sefer Elezkurtaj, Hannes Cash, Daniel Messroghli, Kay Raum, Jonathan Mamou
Summary: This study assessed the performance of a high-frequency linear array transducer in detecting early osteoarthritis. The results showed that the transducer has comparable screening capabilities for early osteoarthritis to research-grade single-element ultrasound and MRI.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Huong Nguyen Minh, Marie Muller, Kay Raum
Summary: The study aims to improve multi-focus imaging technique by correcting phase aberration caused by bone geometry, leading to more accurate and precise measurements of cortical thickness and sound velocity.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Hui Zhang, Nirina Beilfuss, Urszula Zabarylo, Kay Raum, Regina Puts
Summary: In order to rapidly develop precision medicine and patient-oriented treatment strategies, novel technological solutions are urgently needed. This study developed an ultrasound-based tissue engineering acoustophoretic set-up to organize murine mesenchymal stromal cells in an in situ polymerizing 3-D fibrin hydrogel. The structured hydrogels exhibited enhanced expression of osteogenic markers and cell-cycle-progression-associated genes, suggesting the potential for improved regenerative properties in bone healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jean-Gabriel Minonzio, Donatien Ramiandrisoa, Johannes Schneider, Eva Kohut, Melanie Streichhahn, Ulrik Stervbo, Rainer Wirth, Timm Henning Westhoff, Kay Raum, Nina Babel
Summary: Accurate measurement of cortical bone parameters is essential for improving fracture risk assessment and treatment strategies. This study evaluated the performance of a Bi-Directional Axial Transmission (BDAT) device in older patients and found it to be a potentially predictive tool for assessing fracture risk.
Article
Biotechnology & Applied Microbiology
Regina Puts, Aseel Khaffaf, Maria Shaka, Hui Zhang, Kay Raum
Summary: This study investigated the effect of focused low-intensity pulsed ultrasound (FLIPUS) on the survival of starved MLO-Y4 osteocytes in vitro. The results showed that FLIPUS stimulation for 10 minutes led to the extension of dendrites in osteocytes, forming frequent connections with neighboring cells. Additionally, the sonicated cells displayed thick actin bundles and an increase in the expression of connexin 43 (Cx43) and E11 glycoprotein. After stimulation, the expression of cell growth and survival genes as well as genes related to cell-cell communication was augmented. Furthermore, cell viability was improved and ATP release in the medium decreased after sonication. Overall, FLIPUS mitigated apoptosis of starved osteocytes, likely due to the formation of extensive dendritic networks that ensured cell survival.
BIOENGINEERING-BASEL
(2023)
Article
Engineering, Biomedical
Emely Bortel, Liam M. Grover, Neil Eisenstein, Christian Seim, Heikki Suhonen, Alexandra Pacureanu, Peter Westenberger, Kay Raum, Max Langer, Francoise Peyrin, Owen Addison, Bernhard Hesse
Summary: Osteocyte lacunar-canalicular network (LCN) plays a crucial role in bone remodeling and mineral homeostasis, with a highly interconnected structure. The function of LCN is not only to optimize rapid access to bone mineral, but also to maintain high permeability when canaliculi interruptions occur. The structure of LCN can be influenced by anatomical location, subjected loads, and growth rate.
ADVANCED NANOBIOMED RESEARCH
(2022)
Review
Cell & Tissue Engineering
R. Puts, R. Vico, N. Beilfuss, M. Shaka, F. Padilla, K. Raum
Summary: LIPUS is a non-invasive treatment option for bone fractures, but its clinical application faces challenges such as low patient compliance and suboptimal parameter settings, requiring a better definition of the acoustic dose and experimental design. Future non-industry-funded clinical trials are needed to validate the efficacy of LIPUS in fracture healing.
EUROPEAN CELLS & MATERIALS
(2021)
Article
Endocrinology & Metabolism
Gabriele Armbrecht, Huong Nguyen Minh, Jonas Massmann, Kay Raum
Summary: This study conducted in vivo assessment of cortical pore-size distribution in osteoporosis patients using CortBS technology, and compared the discrimination performance with DXA and HR-pQCT. The results showed superior discrimination performance of CortBS, indicating its potential for quantifying microstructural tissue deteriorations in cortical bone.