Article
Medicine, General & Internal
Radana Vrzakova, Vaclav Simanek, Ondrej Topolcan, Vladimir Vurm, David Slouka, Radek Kucera
Summary: This study tested the stability of AMH under various preanalytical conditions and found that AMH remains relatively stable during repeated freezing and thawing in serum, but less stable in plasma. Room temperature was found to be the least suitable condition for sample storage prior to biomarker analysis. Storage stability testing at 5-7 degrees Celsius showed that values decreased over time for all plasma samples but remained stable in serum samples. It was demonstrated that AMH is highly stable under various stress conditions, with the greatest stability observed in serum samples.
Article
Multidisciplinary Sciences
Adel Salimi, Saeid Rahmani, Ali Sharifi-Zarchi
Summary: In this paper, a new method for aggregating multiple reference genes is introduced, which demonstrates better normalization performance. The feasibility and superiority of this method are supported by theoretical and experimental evidence using real data. Moreover, this method has the advantages of efficient computation and easy application.
Article
Pharmacology & Pharmacy
Teresa L. L. Krammer, Marietta Kollars, Paul A. A. Kyrle, Matthias Hackl, Sabine Eichinger, Ludwig Traby
Summary: This study investigates the potential of plasma miRNAs as biomarkers for monitoring antiplatelet treatment. The results show that platelet-enriched miRNAs in the circulation are significantly reduced within 2 hours after P2Y12-mediated platelet inhibition, and higher baseline levels of these miRNAs are associated with a greater reduction upon antiplatelet therapy. Furthermore, the study suggests that miRNAs may be carried by platelet-derived and procoagulant extracellular vesicles.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nikolaos G. Bliziotis, Leo A. J. Kluijtmans, Gerjen H. Tinnevelt, Parminder Reel, Smarti Reel, Katharina Langton, Mercedes Robledo, Christina Pamporaki, Alessio Pecori, Josie Van Kralingen, Martina Tetti, Udo F. H. Engelke, Zoran Erlic, Jasper Engel, Timo Deutschbein, Svenja Noelting, Aleksander Prejbisz, Susan Richter, Jerzy Adamski, Andrzej Januszewicz, Filippo Ceccato, Carla Scaroni, Michael C. Dennedy, Tracy A. Williams, Livia Lenzini, Anne-Paule Gimenez-Roqueplo, Eleanor Davies, Martin Fassnacht, Hanna Remde, Graeme Eisenhofer, Felix Beuschlein, Matthias Kroiss, Emily Jefferson, Maria-Christina Zennaro, Ron A. Wevers, Jeroen J. Jansen, Jaap Deinum, Henri J. L. M. Timmers
Summary: This study aimed to establish signatures for different forms of endocrine hypertension (EHT) and investigate unbiased disease biomarkers. Plasma samples from 13 biobanks across seven countries were analyzed using untargeted NMR metabolomics. While signatures that could differentiate different forms of EHT were found, confounding effects related to sample center and age were also identified. Various approaches were applied to correct these confounding effects, but no biomarkers for differentiating primary hypertension from EHT could be identified.
Article
Oncology
A. Adam-Artigues, I Garrido-Cano, S. Simon, B. Ortega, S. Moragon, A. Lameirinhas, V Constancio, S. Salta, O. Burgues, B. Bermejo, R. Henrique, A. Lluch, C. Jeronimo, P. Eroles, J. M. Cejalvo
Summary: miR-30b-5p expression in breast cancer tissue and plasma differs significantly from healthy samples, with higher levels in plasma of BC patients. This miRNA demonstrates good potential for early diagnosis of breast cancer.
Article
Food Science & Technology
Valeria Tutino, Valentina De Nunzio, Rosa Anna Milella, Marica Gasparro, Anna Maria Cisternino, Isabella Gigante, Elsa Lanzilotta, Palma Aurelia Iacovazzi, Antonio Lippolis, Tamara Lippolis, Maria Gabriella Caruso, Maria Notarnicola
Summary: This study investigates the effects of fresh table grape consumption on circulating levels of common human microRNAs in healthy subjects. The study found that intake of table grape for 21 days led to differential expression of 20 circulating microRNAs, with the majority being down-regulated. Notably, microRNAs related to pathways involved in counteracting cancer development were affected by grape intake.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Environmental Sciences
Hongli Nie, Hua Hu, Zhaoyang Li, Ruixin Wang, Jia He, Peiwen Li, Weiya Li, Xu Cheng, Jun An, Zefang Zhang, Jiao Bi, Jinqiu Yao, Huan Guo, Xiaomin Zhang, Meian He
Summary: This study found significant associations between several plasma metal levels and diabetes risk, as well as certain plasma miRNAs being related to diabetes risk. MiR-122-5p mediated the relationship between titanium and diabetes risk by 48.0%.
ENVIRONMENTAL POLLUTION
(2022)
Article
Multidisciplinary Sciences
Kuno Suzuki, Tatsuya Yamaguchi, Masakazu Kohda, Masami Tanaka, Hiroyuki Takemura, Mitsuru Wakita, Yoko Tabe, Shunsuke Kato, Motomi Nasu, Takashi Hashimoto, Shinji Mine, Nobuko Serizawa, Ko Tomishima, Akihito Nagahara, Takahisa Matsuda, Taiki Yamaji, Shoichiro Tsugane, Yutaka Saito, Hiroyuki Daiko, Takaki Yoshikawa, Ken Kato, Takuji Okusaka, Takahiro Ochiya, Yusuke Yamamoto, Shoji Yotsui, Takashi Yamamoto, Tomoyuki Yamasaki, Hiroshi Miyata, Masayoshi Yasui, Takeshi Omori, Kazuyoshi Ohkawa, Kenji Ikezawa, Tasuku Nakabori, Naotoshi Sugimoto, Toshihiro Kudo, Keiichi Yoshida, Masayuki Ohue, Takashi Nishizawa
Summary: This study investigated the expression of microRNAs (miRNAs) in blood and its relationship with various diseases. The results showed that the use of ethylenediaminetetraacetic acid (EDTA) as an anticoagulant and specific storage conditions can contribute to highly reproducible miRNA expression profiles. Additionally, standardization of these preanalytical conditions can reduce the variation in sample quality across different facilities.
Article
Chemistry, Analytical
Qing Qian, Yuguo Tang, Peng Miao
Summary: In this study, a mass-encoded biosensing approach with duplex-specific nuclease (DSN) mediated signal amplification is developed to address the challenges of low abundance and multiplexing. The proposed method successfully detects multiple targets (such as five miRNAs) with high selectivity and sensitivity. Therefore, it can be utilized as an important and valuable tool for quantifying multiplex miRNAs in disease screening and biomedical investigations.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Aerospace
M. R. Homaeinezhad, M. Nesaeian
Summary: This paper introduces a novel electrohydrodynamic (EHD) actuator system for near space vehicles, which produces thrust by ionizing and accelerating gas. An analytical model called EHD-AM is presented to estimate the thrust produced by EHD actuators in a wire-to-cylinder configuration. The model is validated with experimental data, and the effects of voltage and pressure on thrust are discussed.
Article
Chemistry, Analytical
Jinlan Wei, Sitian He, Yanhua Mao, Longjie Wu, Xinlian Liu, Clement Yaw Effah, Hongchao Guo, Yongjun Wu
Summary: A novel nanoplatform based on molybdenum disulfide nanosheets, utilizing signal amplification and quenching techniques, was developed for the sensitive and specific quantification of microRNA-21 in plasma exosomes. The platform demonstrated successful detection of plasma exosomal microRNA-21 in lung cancer patients, suggesting its potential application in early lung cancer diagnosis.
Article
Medical Laboratory Technology
Christina C. Pierre, Joesph R. Wiencek
Summary: The environment that a clinical specimen is exposed to before arriving at the laboratory can significantly impact the accuracy of test results. Specimens collected offsite and transported to the laboratory may experience varying climate conditions, potentially leading to unreliable results. Even specimens collected within healthcare institutions can be affected by suboptimal storage and transport environments.
CLINICAL BIOCHEMISTRY
(2023)
Article
Biochemical Research Methods
Paolo Verderio, Chiara Maura Ciniselli, Amelie Gaignaux, Marta Pastori, Sabrina Saracino, Olga Kofanova, Fay Betsou
Summary: The annual External Quality Assurance (EQA) program has allowed processing laboratories to assess their processing methods over the past ten years. This study analyzed nearly 1000 EQA scheme/participant data and found that the nucleic acid extraction method and kit, elution buffer, enzymes used, input material quantity, and storage temperature were critical preanalytical factors. The study also observed improvements in laboratory performance over time.
Article
Chemistry, Analytical
Li Ping Cao, Chun Mei Li, Shu Jun Zhen, Cheng Zhi Huang
Summary: A general signal amplifier based on a micelle-supported entropy-driven circuit was designed and prepared for sensitive quantification of biomarkers. The amplifier exhibited excellent sensitivity and selectivity in detecting microRNA-155, showing the potential for the development of DNA nanotechnology in biosensing.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Li Ping Cao, Chun Mei Li, Shu Jun Zhen, Cheng Zhi Huang
Summary: A general signal amplifier based on a micelle-supported entropy-driven circuit (MEDC) was designed for sensitive quantification of biomarkers. The MEDC was triggered by microRNA-155, resulting in dynamic self-assembly of poly-DNA micelles and causing the recovery of cyanine 3 fluorescence.
ANALYTICAL CHEMISTRY
(2023)
Article
Medical Laboratory Technology
Patricia Diana Soerensen, Henry Christensen, Soeren Gray Worsoe Laursen, Christian Hardahl, Ivan Brandslund, Jonna Skov Madsen
Summary: The study evaluated the ability of an AI model to predict cancer risk in primary care patients based on routine blood tests. Results showed that the AI model can provide an easy-to-use risk score for predicting cancer within 90 days, with comparable results to a logistic regression model. The AI model performed slightly better than the LR analysis in terms of AUC, PPV, sensitivity, and specificity in the internal validation cohort, while results were comparable in the out-of-time validation test cohort. The AI risk score may be a valuable tool in clinical decision-making and should be further validated for use in other populations.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Sadaf Kamil, Thomas S. G. Sehested, Kim Houlind, Jens F. Lassen, Gunnar H. Gislason, Helena Dominguez
Summary: This study investigates the population-level incidence of myocardial infarction (MI), heart failure (HF), and cardiovascular mortality in patients with peripheral artery disease (PAD), and finds that the incidence of MI and HF has significantly decreased over time. The results suggest that preventive strategies have improved, likely due to the application of guidelines in clinical care.
EUROPEAN HEART JOURNAL-QUALITY OF CARE AND CLINICAL OUTCOMES
(2023)
Article
Oncology
Louise Raunkilde, Torben Frostrup Hansen, Rikke Fredslund Andersen, Birgitte Mayland Havelund, Caroline Brenner Thomsen, Lars Henrik Jensen
Summary: Although imaging is the gold standard in evaluating the treatment effect in metastatic colorectal cancer, this study found that a reduction in methylated circulating tumor DNA (ctDNA) after one treatment cycle is a better marker of progression-free survival than imaging. Methylated ctDNA can be an early indicator of treatment benefit and is a promising endpoint in clinical trials.
Article
Oncology
Morten Borg, Line Nederby, Sara Witting Christensen Wen, Torben Frostrup Hansen, Anders Jakobsen, Rikke Fredslund Andersen, Ulla Moller Weinreich, Ole Hilberg
Summary: This study assessed the diagnostic properties of a biomarker panel consisting of CYFRA 21-1, CEA, and CA125 for lung cancer detection. The results showed that the biomarkers had high sensitivity and specificity for detecting lung cancer, but their ability to detect early-stage lung cancer was low.
Article
Anesthesiology
Christina Mortensen, Karina Dahl Steffensen, Emma Simonsen, Kamille Herskind, Jonna Skov Madsen, Dorte Aalund Olsen, Ditte Bork Iversen, Troels Korshoj Bergmann, Anton Pottegard, Tore Bjerregaard Stage
Summary: Using a translational approach, this study assessed the utility of neurofilament light chain (NFL) as a biomarker for paclitaxel-induced peripheral neuropathy (PIPN) in a human cell model and in ovarian cancer patients, finding that high serum NFL levels predicted adverse outcomes related to PIPN.
Article
Clinical Neurology
Morten Rune Blichfeldt-Eckhardt, Dorte Aalund Olsen, Rikke Fredslund Andersen, Palle Toft, Laila Bendix
Summary: The purpose of this study was to examine the cerebrospinal fluid (CSF) of patients with post cholecystectomy syndrome and healthy volunteers to determine if there were signs of PGE2 and cytokine mediated central sensitization. The results showed that there were elevated levels of PGE2 metabolite (PGEM) in the CSF of patients with post cholecystectomy syndrome and visceral hyperalgesia, indicating a possible inflammatory component to the pain. Additionally, there was an overrepresentation of the CT genotype in the rs5275 SNP of the Cox2 gene in the pain group.
JOURNAL OF PAIN RESEARCH
(2022)
Article
Oncology
Sara Witting Christensen Wen, Line Nederby, Rikke Fredslund Andersen, Torben Schjodt Hansen, Christa Haugaard Nyhus, Ole Hilberg, Anders Jakobsen, Torben Frostrup Hansen
Summary: Natural killer cell activity (NKA) and methylated HOXA9 circulating tumor DNA (ctDNA) can serve as prognostic biomarkers in patients with non-small cell lung cancer (NSCLC) treated with PD-1/PD-L1 inhibitors. Evaluating the NKA and ctDNA status after one treatment cycle can accurately predict patient survival.
BRITISH JOURNAL OF CANCER
(2023)
Article
Oncology
Ulrik Deding, Lasse Kaalby, Robert Steele, Issam Al-Najami, Morten Kobaek-Larsen, Eva Plantener, Jeppe B. Madsen, Jonna S. Madsen, Thomas Bjorsum-Meyer, Gunnar Baatrup
Summary: Population-based screening for colorectal cancer using FIT is recommended. Detectable faecal haemoglobin can indicate colorectal neoplasia as well as other conditions. A positive FIT predicts an increased risk of death from colorectal cancer but might also predict an increased risk of all-cause mortality.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Medicine, General & Internal
Andrew W. Bradbury, Catherine A. Moakes, Matthew Popplewell, Lewis Meecham, Gareth R. Bate, Lisa Kelly, Ian Chetter, Athanasios Diamantopoulos, Arul Ganeshan, Jack Hall, Simon Hobbs, Kim Houlind, Hugh Jarrett, Suzanne Lockyer, Jonas Malmstedt, Jai Patel, Smitaa Patel, S. Tawqeer Rashid, Athanasios Saratzis, Gemma Slinn, Julian A. Scott, Hany Zayed
Summary: This study compared the effectiveness of vein bypass first and best endovascular treatment first revascularization strategies in patients with chronic limb-threatening ischemia. The results showed that the best endovascular treatment group had a lower rate of amputation and death, suggesting that more patients with chronic limb-threatening ischemia should be considered for a best endovascular treatment first revascularization strategy.
Article
Multidisciplinary Sciences
Ina Mathilde Kjaer, Soren Kahns, Signe Timm, Rikke Fredslund Andersen, Jonna Skov Madsen, Erik Hugger Jakobsen, Tomasz Piotr Tabor, Anders Jakobsen, Troels Bechmann
Summary: This study aimed to investigate the clinical effect of combining delta-tocotrienol with standard neoadjuvant treatment and the possible association between circulating tumor DNA (ctDNA) detection and treatment response. The results showed no difference in response rate or adverse events frequency between the two treatment arms. A multiplex digital droplet polymerase chain reaction (ddPCR) assay for detecting ctDNA in breast cancer patients was developed, and the sensitivity of the assay increased when cancer specific markers were combined with breast tissue specific markers. However, no association between ctDNA status and treatment response was found.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Qihua Tan, Anais Marie Julie Moller, Chuan Qiu, Jonna Skov Madsen, Hui Shen, Troels Bechmann, Jean-Marie Delaisse, Bjarne Winther Kristensen, Hong-Wen Deng, David Karasik, Kent Soe
Summary: Clinical trials have shown that zoledronic acid is effective in preventing bone loss, but its potency varies among patients. Smoking has been reported as a contributing factor to the variation in sensitivity to zoledronic acid. In this study, DNA methylation profiling was performed on blood cells to investigate the molecular basis of smoking-mediated variation in treatment sensitivity. The results revealed significant associations between DNA methylation, smoking, and the half-maximal inhibitory concentration (IC50) of zoledronic acid. Genomic sites associated with bone and body size measures were also found to be enriched near the smoking-associated CpG sites. Furthermore, gene ontology analysis identified several significant functional terms related to ion channel activity and extracellular matrix strength. These findings suggest that smoking affects individual sensitivity to zoledronic acid treatment through epigenetic regulation, highlighting the potential for personalized treatment based on DNA methylation analysis.
CLINICAL EPIGENETICS
(2023)
Article
Pharmacology & Pharmacy
Ann-Cathrine Dalgard Dunvald, Kasper Soltoft, Ekta Sheetal, Soren Andreas Just, Ida Emilie Brejning Frederiksen, Flemming Nielsen, Dorte Aalund Olsen, Jonna Skov Madsen, Oliver Hendricks, Tore Bjerregaard Stage
Summary: Continuous anti-IL-6R therapy in patients with rheumatoid arthritis may cause an acute but transient increase in CYP3A4 activity, which could be attributed to the normalization of inflammation-suppressed CYP activity. Further research is needed to better understand this potential transient effect.
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Louise Bach Callesen, Anders Kindberg Boysen, Rikke Fredslund Andersen, Rikke Beese Dalby, Karen-Lise Garm Spindler
Summary: This exploratory prospective observational pilot study investigated the potential benefits of suggested risk factors in detecting asymptomatic brain metastases in patients with colorectal cancer. The study also examined prognostic biological aspects through translational analysis of plasma samples. The results showed a low incidence of brain metastases in the selected population, indicating that routine MRI may not be necessary for this group.
SCIENTIFIC REPORTS
(2023)
Review
Oncology
Morten Borg, Sara Witting Christensen Wen, Rikke Fredslund Andersen, Signe Timm, Torben Frostrup Hansen, Ole Hilberg
Summary: Lung cancer is the leading cause of cancer-related deaths, and early detection is crucial for improving patient outcomes. Non-invasive diagnostic tools, such as methylated circulating tumor DNA (ctDNA), show potential for lung cancer diagnosis. This systematic review and meta-analysis evaluated the current evidence on the use of methylated ctDNA in blood samples as a diagnostic tool for lung cancer.
Article
Surgery
Diana Hai Yen Tang, Theresa Bruun Ostdal, Anishan Vamadevan, Lars Konge, Kim Houlind, Morten Stadeager, Flemming Bjerrum
Summary: This study aimed to investigate the impact of shorter intervals and longer intervals on proficiency-based laparoscopy simulator training. The results showed no difference between shorter and longer intervals in terms of time to reach proficiency during training and performance in the retention test.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)