Article
Infectious Diseases
Jose Vazquez-Villanueva, Karina Vazquez, Ana Veronica Martinez-Vazquez, Alfredo Wong-Gonzalez, Jesus Hernandez-Escareno, Omar Cabrero-Martinez, Wendy Lizeth Cruz-Pulido, Abraham Guerrero, Gildardo Rivera, Virgilio Bocanegra-Garcia
Summary: This study analyzed 336 samples from a bovine slaughterhouse and found that 83.3% of the samples were positive for E. coli infection. The study revealed that these E. coli isolates exhibited resistance to multiple antibiotics and carried various virulence factors and resistance genes. Therefore, E. coli poses a threat to public health.
Article
Agriculture, Dairy & Animal Science
Toshiyuki Murase, Hiroichi Ozaki
Summary: This study showed that the prevalence of phylogroup F was higher in colibacillosis-related isolates compared to healthy chicken-related isolates, while phylogroups A and B1 were more commonly found in the latter. APEC strains with virulence-associated genes were more prevalent in colibacillosis-related isolates, indicating that phylogroup F can predict colibacillosis in E. coli strains.
Article
Cell Biology
Timothy Kudinha, Fanrong Kong
Summary: The prevalence of ST131, especially among ciprofloxacin resistant isolates, increased from fecal to clinical samples in women, men, and children. ST131 isolates showed higher virulence potential compared to non-ST131 isolates, particularly in urinary samples. There was a strong association between ST131 prevalence and fluoroquinolone resistance, with FQ resistant ST131 isolates harboring more virulence genes.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Microbiology
Victoria Ballen, Yaiza Gabasa, Carlos Ratia, Melany Sanchez, Sara Soto
Summary: This study characterized 376 extraintestinal pathogenic E. coli strains collected from hospitals in Catalonia, Spain, between 2016 and 2017. The strains showed high resistance to several antibiotics and varied biofilm formation ability. Various virulence and antimicrobial resistance genes were detected, including the colibactin-encoding genes associated with colorectal cancer. The study also investigated the relationship between colibactin and biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Beatriz Garcia-Bejar, Izan Garcia de Blas Martin, Maria Arevalo-Villena, Ana Briones Perez
Summary: The study investigates the prevalence of antibiotic resistance in Escherichia coli found in poultry products in Spain, revealing high resistance rates to multiple antibiotics, especially colistin and gentamicin. The majority resistance genes were found to be tet(A) and blaTEM.
Article
Infectious Diseases
Sandra Sevilla-Navarro, Pablo Catala-Gregori, Jan Torres-Boncompte, Maria Teresa Orenga, Josep Garcia-Llorens, Veronica Cortes
Summary: Antimicrobial resistance poses a significant threat to global health, and poultry products are a major source of transmission. Escherichia coli is the primary cause of mortality in the poultry industry, with recurrent outbreaks of multidrug resistant strains. Understanding the burden of resistance is a major challenge in tackling AMR, with monitoring through phenotypic characterization being a key strategy. This study aimed to monitor the resistance of E. coli strains isolated from the poultry sector, providing information on the magnitude and trends of resistance.
Article
Microbiology
Anna Dziuba, Sylwia Dzierzak, Anna Sodo, Monika Wawszczak-Kasza, Katarzyna Zegadlo, Jakub Bialek, Natalia Zych, Wojciech Kiebzak, Jaroslaw Matykiewicz, Stanislaw Gluszek, Wioletta Adamus-Bialek
Summary: In this study, we demonstrated significant differences between urine-derived E. coli and other clinical E. coli isolates in terms of pathogenicity. We suggest excluding penicillins from use in E. coli infection at this time and monitoring strains with a high pathogenicity potential.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Shaqiu Zhang, Shuling Chen, Mujeeb Ur Rehman, Hong Yang, Zhishuang Yang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Dekang Zhu, Xinxin Zhao, Ying Wu, Qiao Yang, Juan Huan, Xumin Ou, Sai Mao, Qun Gao, Di Sun, Bin Tian, Anchun Cheng
Summary: The study revealed that healthy waterfowls harbor E. coli with multiple ARGs and various ExPEC-associated VAGs. The findings suggest the need for regulated antibiotic use to prevent the direct or indirect spread of these resistant and potentially pathogenic microbes to the natural environment.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Infectious Diseases
Yijun Ding, Jinjing Zhang, Kaihu Yao, Wei Gao, Yajuan Wang
Summary: This study assessed the antimicrobial resistance and molecular characteristics of E. coli ST1193 isolates causing neonatal bloodstream infections and meningitis in China, revealing that ST1193 isolates were often multidrug-resistant. Further multicenter studies are needed to control the spread of this emerging fluoroquinolone-resistant clone.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Reed Woyda, Adelumola Oladeinde, Zaid Abdo
Summary: Pathogenic Escherichia coli causes disease in humans and food-producing animals. Its pathogenesis depends on virulence factors and antimicrobial resistance genes. Contaminated and undercooked food products are highly associated with food-borne outbreaks. It is important to identify the genetic factors that contribute to the virulence and antimicrobial resistance of E. coli.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Francesca Palma, Federica Dell'Annunziata, Veronica Folliero, Francesco Foglia, Roberta Della Marca, Carla Zannella, Anna De Filippis, Gianluigi Franci, Massimiliano Galdiero
Summary: Multidrug resistance is a global concern, and there is a need for new intervention strategies to combat nosocomial infections. Drug repurposing, such as using Cupferron as an antimicrobial agent, could provide a cost-effective alternative. The study demonstrated that Cupferron has bacteriostatic action against both multidrug-resistant and sensitive strains of bacteria, and it also affects biofilm production, oxidative stress, and bacterial motility.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Microbiology
Camille Lucas, Sabine Delannoy, Catherine Schouler, Rozenn Souillard, Laetitia Le Devendec, Pierrick Lucas, Alassane Keita, Patrick Fach, Julie Puterflam, Stephanie Bougeard, Isabelle Kempf
Summary: This study identified relevant genetic markers associated with avian pathogenic E. coli (APEC) using high-throughput PCR and a machine learning procedure, which is crucial for better control of avian colibacillosis.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Maria Teresa Llorente, Raquel Escudero, Raquel Ramiro, Maria Antonia Remacha, Rocio Martinez-Ruiz, Fatima Galan-Sanchez, Monica de Frutos, Matilde Elia, Isabel Onrubia, Sergio Sanchez
Summary: This study aimed to investigate the occurrence of enteroaggregative Escherichia coli (EAEC) in patients with non-travel related diarrhea in Spain and to characterize the molecular features of EAEC strains associated with illness in this high-income setting. The results showed that EAEC was the only bacterial enteric pathogen detected in a significant proportion of cases of endemic diarrhea in Spain, especially in children under 5 years old. Some of these strains also had a potential pathogenicity in the urinary/systemic system.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Sifuna Anthony Wawire, Oleg N. Reva, Thomas J. O'Brien, Wendy Figueroa, Victor Dinda, William A. Shivoga, Martin Welch
Summary: This study used whole genome sequencing to investigate antimicrobial resistance and virulence-associated genes in clinical and municipal wastewater Escherichia coli isolates from Kakamega, west Kenya. It found that clinical isolates had more genes required for in-host survival, while plasmids from clinical isolates were enriched in AMR and VA genes, suggesting plasmids as a primary means for acquisition and dissemination of these genes among strains.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Ecology
Ayush Pathak, Daniel C. Angst, Ricardo Leon-Sampedro, Alex R. Hall
Summary: Some bacterial resistance mechanisms can degrade antibiotics, potentially protecting neighboring susceptible cells. However, the effects of such mechanisms on bacterial communities of more than two species are not well understood. By conducting experiments on multispecies communities, we found that resistance in one species reduced antibiotic inhibition of other species, but the extent of benefit varied among species.
Article
Public, Environmental & Occupational Health
Daniel Prieto-Alhambra, Kristin Kostka, Talita Duarte-Salles, Albert Prats-Uribe, Anthony Sena, Andrea Pistillo, Sara Khalid, Lana Lai, Asieh Golozar, Thamir M. Alshammari, Dalia Dawoud, Fredrik Nyberg, Adam Wilcox, Alan Andryc, Andrew Williams, Anna Ostropolets, Carlos Areia, Chi Young Jung, Christopher Harle, Christian Reich, Clair Blacketer, Daniel Morales, David A. Dorr, Edward Burn, Elena Roel, Eng Hooi Tan, Evan Minty, Frank DeFalco, Gabriel de Maeztu, Gigi Lipori, Heba Alghoul, Hong Zhu, Jason Thomas, Jiang Bian, Jimyung Park, Jordi Martinez Roldan, Jose Posada, Juan M. Banda, Juan P. Horcajada, Julianna Kohler, Karishma Shah, Karthik Natarajan, Kristine Lynch, Li Liu, Lisa Schilling, Martina Recalde, Matthew Spotnitz, Mengchun Gong, Michael Matheny, Neus Valveny, Nicole Weiskopf, Nigam Shah, Osaid Alser, Paula Casajust, Rae Woong Park, Robert Schuff, Sarah Seager, Scott DuVall, Seng Chan You, Seokyoung Song, Sergio Fernandez-Bertolin, Stephen Fortin, Tanja Magoc, Thomas Falconer, Vignesh Subbian, Vojtech Huser, Waheed-Ul-Rahman Ahmed, William Carter, Yin Guan, Yankuic Galvan, Xing He, Peter Rijnbeek, George Hripcsak, Patrick Ryan, Marc Suchard, Daniel Prieto-Alhambra
Summary: This study utilizes the Observational Health Data Sciences and Informatics (OHDSI) framework to standardize and analyze real-world data on COVID-19. The findings reveal global variations in comorbidities, symptoms, and clinical presentations among patients. The study provides a global, multi-center perspective on the progression, management, and evolution of COVID-19.
CLINICAL EPIDEMIOLOGY
(2022)
Editorial Material
Infectious Diseases
Emilia Cercenado, Jesus Rodriguez-Bano, Jose Luis Alfonso, Esther Calbo, Luis Escosa, Aurora Fernandez-Polo, Julio Garcia-Rodriguez, Jose Garnacho, Maria Victoria Gil-Navarro, Santiago Grau, Carlota Gudiol, Juan Pablo Horcajada, Nieves Larrosa, Carmen Martinez, Jose Molina, Xavier Nuvials, Antonio Oliver, Jose Ramon Pano-Pardo, Maria Teresa Perez-Rodriguez, Paula Ramirez, Pedro Rey-Biel, Pablo Vidal, Pilar Retamar-Gentil
Summary: In 2012, consensus recommendations were published by multiple Spanish societies on the implementation of antimicrobial stewardship (AMS) programs in acute care hospitals. However, there was a need for further guidance on AMS activities for specific patient populations or circumstances. Therefore, a panel of experts was assembled to review available information and provide guidance recommendations on these topics.
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2023)
Article
Immunology
Jose Avendano-Ortiz, Manuel Ponce-Alonso, Emilio Llanos-Gonzalez, Hugo Barragan-Prada, Raquel Barbero-Herranz, Roberto Lozano-Rodriguez, Francesc J. Marquez-Garrido, Jose Maria Hernandez-Perez, Maria-Isabel Morosini, Rafael Canton, Rosa del Campo, Eduardo Lopez-Collazo
Summary: We investigated the effects of different lipopolysaccharide (LPS) modifications on colistin resistance and found that adding Ara4N to LPS increases the survival of Klebsiella pneumoniae in the human body and elicits a stronger inflammatory response.
INFECTION AND IMMUNITY
(2023)
Article
Infectious Diseases
D. Fresan, S. Luque, A. Benitez-Cano, L. Sorli, M. Milagro Montero, M. De-Antonio, N. Prim, V. Vega, J. P. Horcajada, S. Grau
Summary: Administering ceftazidime/avibactam by continuous infusion can achieve the desired drug concentration and therapeutic target in most patients, even at lower doses. The combination of continuous infusion and therapeutic drug monitoring can be a useful tool for reducing antimicrobial-related adverse effects and treatment costs.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Pilar Retamar-Gentil, Rafael Canton, Vicente Abril Lopez de Medrano, Jose Barberan, Andres Canut Blasco, Carlos Duenas Gutierrez, Carolina Garcia-Vidal, Nieves Larrosa Escartin, Jaime Lora-Tamayo, Francisco Javier Martinez Marcos, Carlos Martin Ruiz, Juan Pasquau Liano, Pedro Rascado, Oscar Sanz Pelaez, Genoveva Yague Girao, Juan P. Horcajada
Summary: This work presents the views of a group of experts on resistance to antimicrobial agents in Gram-negative bacilli, including the current epidemiology, its relation to clinical practice, and new therapies based on evidence. After reviewing the most relevant evidence, data on these aspects were presented to experts in infectious diseases, clinical microbiology, internal medicine, intensive care medicine, anaesthesiology, and hospital pharmacy, leading to conclusions on the matter.
REVISTA ESPANOLA DE QUIMIOTERAPIA
(2023)
Article
Multidisciplinary Sciences
Maria Milagro Montero, Max Hardy-Werbin, Soledad Gonzalez-Gallardo, Erica Torres, Rebeca Rueda, Irene Hannet, James T. Kirk, Thomas D. Yager, Krupa Navalkar, Maria del Mar Arenas, Itziar Arietta-Aldea, Silvia Castaneda, Joan Gomez-Junyent, Silvia Gomez-Zorrilla, Roberto Guerri-Fernandez, Francisca Sanchez-Martinez, Immaculada Lopez-Montesinos, Ivan Pelegrin, Elena Sendra, Luisa Sorli, Judith Villar-Garcia, Beatriz Bellosillo, Juan Pablo Horcajada
Summary: Evaluation of SeptiCyte RAPID as a triaging tool for COVID-19 patients found that it can stratify cases according to clinical severity. The tool showed better discrimination than other biomarkers in predicting the need for ICU admission in COVID-19 patients.
SCIENTIFIC REPORTS
(2023)
Review
Infectious Diseases
Daniel Echeverria-Esnal, Luisa Sorli, Maria Eugenia Navarrete-Rouco, Nuria Prim, Jaime Barcelo-Vidal, David Conde-Estevez, Maria Milagro Montero, Clara Martin-Ontiyuelo, Juan Pablo Horcajada, Santiago Grau
Summary: This article reviews the relevant aspects of ampicillin-resistant and vancomycin-susceptible Enterococcus faecium (EfARSV) bacteremia, including microbiology, gastrointestinal tract colonization and invasion, antibiotic resistance, epidemiology, risk factors, mortality, and treatment. However, the most appropriate treatment for this infection remains uncertain due to limited data.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2023)
Article
Public, Environmental & Occupational Health
Friederike Maechler, Frank Schwab, Sonja Hansen, Michael Behnke, Marc J. Bonten, Rafael Canton, Cristina Diaz Agero, Carolina Fankhauser, Stephan Harbarth, Benedikt D. Huttner, Axel Kola, Petra Gastmeier
Summary: This study aimed to quantify the time delay between screening and initiation of contact isolation for carriers of ESBL-E. The results showed that despite extensive surveillance screening, almost one-third of ESBL-E carriers were not isolated, impeding timely implementation of targeted contact isolation.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Infectious Diseases
Juan Antonio Castillo-Polo, Marta Hernandez-Garcia, Maria Isabel Morosini, Blanca Perez-Viso, Cruz Soriano, Raul De Pablo, Rafael Canton, Patricia Ruiz-Garbajosa
Summary: The study aimed to describe the phenotypic and genotypic characteristics of ceftazidime/avibactam- and cefiderocol-resistant KPC-Klebsiella pneumoniae (KPC-Kp) during an outbreak in a medical ICU. The KPC-Kp isolates involved in the outbreak were found to belong to the high-risk clone ST307 and carried the KPC + CTX-M-15 genes. Cloning experiments showed that specific gene mutations may contribute to resistance to ceftazidime/avibactam and cefiderocol.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Medicine, General & Internal
Diana Badenes Bonet, Oswaldo Antonio Caguana Velez, Xavier Duran Jorda, Merce Comas Serrano, Margarita Posso Rivera, Mireia Admetllo, Anna Herranz Blasco, Elisa Cuadrado Godia, Ester Marco Navarro, Gemma Martin Ezquerra, Zenaida Pineiro Aguin, Maria Cinta Cumpli Gargallo, Jose Gregorio Gonzalez Garcia, Eva Balcells Vilarnau, Diego Rodriguez Chiaradia, Xavier Castells, Joaquim Gea, Juan P. Horcajada, Judit Villar-Garcia
Summary: This study aimed to identify risk factors for the presence of post-acute sequelae of SARS-CoV-2 (PASC) and describe the profile of persistent symptoms in a multidisciplinary Post-Coronavirus Disease-19 (COVID-19) Unit. The results showed that treatment with dexamethasone and remdesivir was associated with shorter duration of symptoms, and female gender, obesity, asthma, and disease severity were identified as risk factors for PASC.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Microbiology
Teresa M. Coque, Rafael Canton, Ana Elena Perez-Cobas, Miguel D. Fernandez-de-Bobadilla, Fernando Baquero
Summary: Antimicrobial resistance (AMR) is a global health challenge that has been studied and approached from various perspectives, including patient safety, molecular epidemiology, public health, health economics, community psychology, and history of science. However, there is a lack of dialogue between different stakeholders, hindering the development and implementation of effective strategies to combat AMR.
Article
Infectious Diseases
Rafael Canton, Thomas Gottlieb, Geoffrey W. Coombs, Patrick C. Y. Woo, Tony M. Korman, Maria Garcia-Castillo, Denise Daley, Karri A. Bauer, Michael Wong, Dominik J. Wolf, Fakhar Siddiqui, Mary Motyl
Summary: Antimicrobial resistance (AMR) is a major global public health threat, particularly affecting patients in resource-poor settings. Various surveillance programs exist worldwide, and one of the largest is the SMART program, which has been running for 20 years. The SMART database includes data from nearly 500,000 isolates from over 60 countries, and can help monitor and guide clinical treatment.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Review
Medicine, General & Internal
Francisco Javier Candel, Miguel Salavert, Miren Basaras, Marcio Borges, Rafael Canton, Emilia Cercenado, Catian Cilloniz, Angel Estella, Juan M. Garcia-Lechuz, Jose Garnacho Montero, Federico Gordo, Agustin Julian-Jimenez, Francisco Javier Martin-Sanchez, Emilio Maseda, Mayra Matesanz, Rosario Menendez, Manuel Miron-Rubio, Raul Ortiz de Lejarazu, Eva Polverino, Pilar Retamar-Gentil, Luis Alberto Ruiz-Iturriaga, Susana Sancho, Leyre Serrano
Summary: This paper reviews important issues in the approach to community-acquired pneumonia, including updates in etiology, diagnostic guidance, treatment, management, and prevention.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Health Care Sciences & Services
Adela Benitez-Cano, Silvia Bermejo, Sonia Luque, Luisa Sorli, Jesus Carazo, Irene Zaragoza, Isabel Ramos, Jordi Valles, Juan P. Horcajada, Ramon Adalia
Summary: This study evaluated the clinical, microbiological, and treatment characteristics of severe postoperative pneumonia and tracheobronchitis. The findings showed that surgical patients are at high risk for early onset severe pneumonia and tracheobronchitis, with a high proportion of Gram-negative and multi-drug resistant bacteria.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Health Care Sciences & Services
Miquel Angel Sastre-Femenia, Almudena Fernandez-Munoz, Maria Antonia Gomis-Font, Biel Taltavull, Carla Lopez-Causape, Jorge Arca-Suarez, Luis Martinez-Martinez, Rafael Canton, Nieves Larrosa, Jesus Oteo-Iglesias, Laura Zamorano, Antonio Oliver
Summary: A high-resolution analysis of resistance patterns, genomic epidemiology, and resistome of Pseudomonas aeruginosa in Spain over a five-year period reveals a general decrease in resistance but an increase in the proportion of XDR strains producing carbapenemases, with the high-risk clone ST235 becoming more prevalent.
LANCET REGIONAL HEALTH-EUROPE
(2023)