Article
Multidisciplinary Sciences
Shao-Hui Pan, Ning Zhao, Xiang Feng, Ying Jie, Zi-Bing Jin
Summary: The study developed a stepwise strategy to generate chemically induced neural crest cells (ciNCCs) and chemically induced corneal endothelial cells (ciCECs) from mouse fibroblasts using defined small molecules. The resulting ciCECs exhibited consistent gene expression profiles and self-renewal capacity to primary CECs, and could be cultured for as long as 30 passages while retaining their features. Transplantation of these ciCECs into an animal model reversed corneal opacity, providing an alternative cell source for regeneration of corneal endothelia and tissues derived from neural crest.
Article
Cell & Tissue Engineering
Ioannis J. Limnios, Yu-Qian Chau, Stuart J. Skabo, Denver C. Surrao, Helen C. O'Neill
Summary: This study developed a rapid and efficient method for producing retinal RPE cells from human embryonic stem cells (hESCs) using small molecules. The RPE cells exhibited key morphological, molecular, and functional characteristics of endogenous RPE, showing potential for clinical-grade cell production to treat AMD.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Pyry Gronroos, Tanja Ilmarinen, Heli Skottman
Summary: This study successfully generated CEnC-like cells from human pluripotent stem cells using a defined differentiation method involving small molecule induction and retinoic acid. The induced cells exhibited morphological characteristics and expression of CEnC markers, demonstrating the potential of this method for regenerative medicine applications in corneal endothelial cells.
Article
Cell Biology
Bowen Zhang, Xumin Wu, Guicheng Zi, Lijuan He, Sihan Wang, Lin Chen, Zeng Fan, Xue Nan, Jiafei Xi, Wen Yue, Lei Wang, Liu Wang, Jie Hao, Xuetao Pei, Yanhua Li
Summary: By optimizing the culture protocol, a large number of megakaryocytes were successfully generated from human embryonic stem cells, confirming their biosafety and demonstrating their ability to form platelets. This study further enhances the clinical applications of megakaryocytes or platelets derived from pluripotent stem cells.
CELL PROLIFERATION
(2021)
Article
Cell & Tissue Engineering
Michael J. Herriges, Maria Yampolskaya, Bibek R. Thapa, Jonathan Lindstrom-Vautrin, Feiya Wang, Jessie Huang, Cheng-Lun Na, Liang Ma, McKenna M. Montminy, Pushpinder Bawa, Carlos Villacorta-Martin, Pankaj Mehta, Darrell N. Kotton
Summary: This study successfully differentiated murine pluripotent stem cells into lung epithelial progenitors and engrafted them into the injured distal lung epithelium. The transplanted cells matured and assumed the phenotypes of specific lung cell types. The study found that the donor-derived cells retained their mature phenotypes, proliferated, and differentiated.
Article
Cell Biology
Jose Manuel Inacio, Joao von Gilsa Lopes, Ana Mafalda Silva, Fernando Cristo, Sara Marques, Matthias E. Futschik, Jose Antonio Belo
Summary: Deciphering the clues of a regenerative mechanism for the mammalian adult heart, particularly focusing on the role of DAND5, could potentially lead to the development of more effective treatment options for heart failure. Further research on the transcriptional profiling sequencing and quantitative RT-PCR assays of DAND5 may provide insights into the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems for clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Article
Biochemistry & Molecular Biology
Tomotaka Kitamura, Masayasu Misu, Masahide Yoshikawa, Yukiteru Ouji
Summary: This study demonstrates that lung-derived matrix sheets (LMSs) can induce differentiation of embryonic stem (ES) cells into lung cells, as indicated by the upregulation of lung-related cell markers.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Marcin Michalik, Patrycja Wieczorek, Piotr Czekaj
Summary: The effectiveness of differentiating hAECs into hepatic cells can be enhanced by culturing them with high concentrations of HGF or EGF. HGF is more effective in increasing the expression of liver-specific genes, while EGF stimulates the differentiation process more efficiently, resulting in more hepatocyte-like cells capable of synthesizing alpha-fetoprotein.
Article
Biology
Evelien Eenjes, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Gabriela G. Edel, Floor Benthem, Lisette de Kreij-de Bruin, Marco Schnater, Dick Tibboel, Jennifer Collins, Robbert J. Rottier
Summary: SOX21 patterns the airway epithelium by antagonizing SOX2, regulating differentiation of airway progenitor cells. It inhibits basal to ciliated cell differentiation in both development and adult tracheal epithelium. The interplay between SOX2 and SOX21 is context and concentration dependent, crucial for regulating differentiation of the airway epithelium.
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Multidisciplinary Sciences
Mingsen Li, Huaxing Huang, Lingyu Li, Chenxi He, Liqiong Zhu, Huizhen Guo, Li Wang, Jiafeng Liu, Siqi Wu, Jingxin Liu, Tao Xu, Zhen Mao, Nan Cao, Kang Zhang, Fei Lan, Junjun Ding, Jin Yuan, Yizhi Liu, Hong Ouyang
Summary: This study reveals that adult stem cell identity and homeostasis are regulated by lineage-restricted transcriptional regulatory networks, with an emphasis on the critical roles of RUNX1 and SMAD3 in maintaining corneal epithelial identity and homeostasis. Furthermore, the cooperation of RUNX1, PAX6, and SMAD3 establishes a core transcription regulatory circuitry for governing lineage-specific cis-regulatory atlas in limbal stem/progenitor cells.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Hong Thi Nguyen, Kasem Theerakittayakorn, Sirilak Somredngan, Apichart Ngernsoungnern, Piyada Ngernsoungnern, Pishyaporn Sritangos, Mariena Ketudat-Cairns, Sumeth Imsoonthornruksa, Juthaporn Assawachananont, Nattawut Keeratibharat, Rangsirat Wongsan, Ruttachuk Rungsiwiwut, Chuti Laowtammathron, Nguyen Xuan Bui, Rangsun Parnpai
Summary: This study reveals that WJ-MSCs have the potential to differentiate into CECs, which would be beneficial for further applications in LSCD treatment therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yan Li, Zhiyu Xia, Haihong Yin, Youran Dai, Feixue Li, Jianming Chen, Mengsheng Qiu, Huarong Huang
Summary: A simple and efficient method for inducing mouse embryonic stem cells into primitive endoderm cells was reported in this study, and it was found that the Wnt signaling pathway played a critical role in this process.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yue Xiao, Min Hu, Qiyan Lin, Ting Zhang, Siying Li, Linjuan Shu, Xiuli Song, Xiaoyong Xu, Wentong Meng, Xue Li, Hong Xu, Xianming Mo
Summary: This study reveals that Dopey2 and Pcdh7 regulate the proliferation and differentiation of neural stem cells and progenitors during embryonic neurogenesis, leading to the proper size and architecture of zebrafish brains. Dopey2 promotes the proliferation of neural stem cells/progenitors, while Pcdh7 promotes their differentiation. These proteins work together to orchestrate the development and arrangement of neural cells in zebrafish embryonic brains.
Article
Ophthalmology
Hui Guo, Kunpeng Pang, Yan Wei, Cuihua Yi, Xinyi Wu
OPHTHALMIC RESEARCH
(2015)
Article
Multidisciplinary Sciences
Kunpeng Pang, Liqun Du, Kai Zhang, Chenyang Dai, Chengqun Ju, Jing Zhu, Xinyi Wu
Article
Engineering, Biomedical
Kunpeng Pang, Liqun Du, Xinyi Wu
Article
Ophthalmology
Liqun Du, Xinyi Wu, Kunpeng Pang, Yongmei Yang
BRITISH JOURNAL OF OPHTHALMOLOGY
(2011)
Article
Ophthalmology
Li-Qun Du, Xin-Yi Wu, Ming-Chun Li, Shen-Guo Wang, Kun-Peng Pang
OPHTHALMIC RESEARCH
(2008)
Article
Cell & Tissue Engineering
Kai Zhang, Kunpeng Pang, Xinyi Wu
STEM CELLS AND DEVELOPMENT
(2014)
Article
Cell & Tissue Engineering
Jing Zhu, Le-yi Wang, Chong-yun Li, Jia-yin Wu, Yu-ting Zhang, Kun-peng Pang, Yan Wei, Li-qun Du, Mei Liu, Xin-yi Wu
Article
Engineering, Biomedical
Qian Wang, Hang Zhou, Yongqiang Sun, Chengbo Cao, Kunpeng Pang
JOURNAL OF BIOMATERIALS APPLICATIONS
(2020)
Article
Ophthalmology
Chenshuang Li, Kunpeng Pang, Liqun Du, Xinyi Wu
Summary: The study evaluates the efficacy and safety of novel tricyclic corneal stroma injection (TCSI) voriconazole for fungal keratitis treatment. Results showed that TCSI group had improved visual acuity and shorter time to re-epithelialization compared to the control group, with no significant differences in size of infiltrate or scar, corneal ECD, or IOP. TCSI may be a minimally invasive, safe, and effective adjuvant treatment modality for fungal keratitis.
JOURNAL OF OPHTHALMOLOGY
(2021)
Meeting Abstract
Ophthalmology
Kunpeng Pang, Sanming Li, Shuyan Zhu, Kathryn Pate, Jia Yin
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Ophthalmology
Kai Zhan, Xiao-Xiao Ren, Ping Li, Kun-Peng Pang, Hong Wang
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
(2019)
Article
Immunology
Chengqun Ju, Li Gao, Xinyi Wu, Kunpeng Pang
INDIAN JOURNAL OF MEDICAL RESEARCH
(2012)