Article
Biochemistry & Molecular Biology
Levi Groen, Iris Kloots, David Englert, Kelly Seto, Lana Estafanos, Paul Smith, Gerald W. Verhaegh, Niven Mehra, Jack A. Schalken
Summary: The clinical utility of circulating tumor cells (CTC) as a non-invasive multipurpose biomarker is well recognized. The earliest methods for enriching CTCs relied on antibody-based positive selection, but it has limitations in capturing the heterogeneity of cancer. This study used the recently FDA-approved Parsortix (R) technology to enrich CTCs from prostate cancer patients and found that targeted CTC transcriptome profiling may be predictive of therapy response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Lorena Alexandra Lisencu, Eduard-Alexandru Bonci, Alexandru Irimie, Ovidiu Balacescu, Cosmin Lisencu
Summary: This review discusses the role of circulating tumor cells (CTCs) in chemoresistance in metastatic breast cancer patients. It highlights that CTCs with stem cell and mesenchymal features are aggressive subtypes that can survive chemotherapy, leading to chemoresistance and disease progression. Hemodynamic shear stress and dynamic changes among CTCs are also important factors in disease progression.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Oncology
Ami N. Shah, Kristen J. Carroll, Lorenzo Gerratana, Chenyu Lin, Andrew A. Davis, Qiang Zhang, Saya Jacob, Brian Finkelman, Youbin Zhang, Wenen Qiang, Paolo D'Amico, Carolina Reduzzi, William J. Gradishar, Amir Behdad, Massimo Cristofanilli
Summary: Data shows that premenopausal women diagnosed with metastatic breast cancer have unique clinical, pathological, and molecular features compared to their postmenopausal counterparts, potentially impacting treatment options.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Oncology
Florian Reinhardt, Luisa Coen, Mahdi Rivandi, Andre Franken, Eunike Sawitning Ayu Setyono, Tobias Lindenberg, Jens Eberhardt, Tanja Fehm, Dieter Niederacher, Franziska Knopf, Hans Neubauer
Summary: DanioCTC is a novel workflow that injects patient-derived CTCs into zebrafish, enabling studies on the metastatic potential of these rare tumor cells.
Article
Oncology
Mingxiong Sheng, Shanming Guo, Chunxiao Liu
Summary: The study demonstrated that CTCs are valuable prognostic and treatment response markers for mPCA patients. ADT plus cryosurgery reduced CTCs and prolonged PFS compared to ADT alone, particularly in patients with low metastatic volume.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2021)
Article
Oncology
Elena Muraro, Fabio Del Ben, Matteo Turetta, Daniela Cesselli, Michela Bulfoni, Rita Zamarchi, Elisabetta Rossi, Simon Spazzapan, Riccardo Dolcetti, Agostino Steffan, Giulia Brisotto
Summary: In this study, the relationship between circulating tumor cells (CTCs) and peripheral T-cell immunity in metastatic breast cancer patients was investigated using liquid biopsy. The combined analysis of CTCs and circulating anti-tumor T-cell immunity may serve as a valuable immune-oncological biomarker in the field of liquid biopsy.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Dorothea Sonja Schott, Monika Pizon, Ulrich Pachmann, Katharina Pachmann, Rainer Schobert, Andrea Wittig, Matthias Maeurer
Summary: This study investigated changes in CETC and cCSC numbers in breast cancer patients before and after radiotherapy, finding that CETC numbers correlated with tumor aggressiveness and cCSCs could be successfully monitored during treatment. Patients treated with neoadjuvant chemotherapy had higher CETC and tumorsphere numbers compared to those treated with adjuvant chemotherapy.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Oncology
Alexia Lopresti, Claire Acquaviva, Laurys Boudin, Pascal Finetti, Severine Garnier, Anais Aulas, Maria Lucia Liberatoscioli, Olivier Cabaud, Arnaud Guille, Alexandre de Nonneville, Quentin Da Costa, Emilie Denicolai, Jihane Pakradouni, Anthony Goncalves, Daniel Birnbaum, Francois Bertucci, Emilie Mamessier
Summary: In this study, atypical cells in the blood of metastatic breast cancer patients were isolated and analyzed using a micro-filtration technic. Three subsets of circulating tumor cells were identified, each with different prognostic values. Moreover, a subset of CTCs was found to be a potential stratification tool for selecting patients for early clinical trials. This study highlights the heterogeneity of CTCs and their potential as a non-invasive tool for precision medicine.
Review
Oncology
Serena Capici, Luca Carlofrancesco Ammoni, Nicole Meli, Viola Cogliati, Francesca Fulvia Pepe, Francesca Piazza, Marina Elena Cazzaniga
Summary: This review focuses on new molecular biomarkers and emerging therapeutic approaches in triple-negative breast cancer (TNBC), providing a general overview of ongoing clinical trials, future perspectives, and differences in approval by American and European regulatory authorities. TNBC is a complex and heterogeneous type of tumor characterized by poor prognosis, aggressive behavior, and lack of effective therapeutic strategies. The identification of new biomarkers and molecular signatures is leading to the development of new therapeutic strategies for TNBC, including immunotherapy, targeted therapy, and antibody-drug conjugates.
Article
Cell Biology
Chrysoula Tsirigoti, Mohamad Moustafa Ali, Varun Maturi, Carl-Henrik Heldin, Aristidis Moustakas
Summary: The transcription factor SNAI1 plays an important role in breast cancer by regulating cellular transformation, differentiation and migration. Loss of SNAI1 function may lead to changes in the phenotype of breast cancer cells and an increase in differentiation potential, possibly through the dual transcriptional action of FOXA1 and AR.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Kirby P. Gardner, Massimo Cristofanilli, Saranya Chumsri, Rena Lapidus, Cha-Mei Tang, Ashvathi Raghavakaimal, Daniel L. Adams
Summary: The usage of beta blockers in breast cancer patients is associated with a reduction in distant metastases, cancer recurrence, and cancer mortality. Studies indicate that the adrenergic pathway plays a role in the promotion of tumor growth and cancer cell dissemination. Cancer-associated macrophage-like cells (CAMLs) originating from tumors may facilitate the spread of circulating tumor cells (CTCs). The expression of the Beta-2 adrenergic receptor (B2AR) in CAMLs is closely linked to their presence in the circulation, size, as well as the progression-free survival and overall survival of breast cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Aleix Prat, Fara Braso-Maristany, Olga Martinez-Saez, Esther Sanfeliu, Youli Xia, Meritxell Bellet, Patricia Galvan, Debora Martinez, Tomas Pascual, Mercedes Marin-Aguilera, Anna Rodriguez, Nuria Chic, Barbara Adamo, Laia Pare, Maria Vidal, Mireia Margeli, Ester Ballana, Marina Gomez-Rey, Mafalda Oliveira, Eudald Felip, Judit Matito, Rodrigo Sanchez-Bayona, Anna Sunol, Cristina Saura, Eva Ciruelos, Pablo Tolosa, Montserrat Munoz, Blanca Gonzalez-Farre, Patricia Villagrasa, Joel S. Parker, Charles M. Perou, Ana Vivancos
Summary: This study uses shallow WGS to create machine learning signatures to identify tumor phenotypes and predict therapy response in metastatic breast cancer patients. Machine learning multi-gene signatures obtained from ctDNA can identify complex biological features similar to those obtained from direct tumor tissue DNA or RNA profiling.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Chun Liu, Miao Li, Zhao-Xia Dong, Dong Jiang, Xiaojing Li, Shuibin Lin, Demeng Chen, Xuenong Zou, Xing-Ding Zhang, Gary D. Luker
Summary: The study showed that breast cancer cells behave differently in environments with different stiffness: cells tend to proliferate in soft environments and migrate in stiff environments. Breast cancer cells switch metabolic pathways and gene expression in response to varying environmental stiffness.
ACTA BIOMATERIALIA
(2021)
Article
Oncology
Angeleke Saridakis, Elizabeth R. Berger, Malini Harigopal, Tristen Park, Nina Horowitz, Justin Le Blanc, Gregory Zanieski, Anees Chagpar, Rachel Greenup, Mehra Golshan, Donald R. Lannin
Summary: Invasive apocrine carcinomas have more aggressive features than non-apocrine carcinomas but the breast cancer-specific survival is the same. Half of these apocrine tumors are triple negative but these have more favorable features and much better survival than non-apocrine triple-negative cancers. The characteristics of apocrine carcinomas vary dramatically by molecular type, with triple-negative apocrine patients having better survival outcomes compared to non-apocrine triple-negative patients, while luminal apocrine patients have worse survival outcomes compared to non-apocrine luminal patients.
ANNALS OF SURGICAL ONCOLOGY
(2021)
Article
Oncology
Cvetka Grasic Kuhar, Jernej Silvester, Marina Mencinger, Tanja Ovcaricek, Maja Cemazar, Simona Miceska, Ziva Modic, Anamarija Kuhar, Tanja Jesenko, Veronika Kloboves Prevodnik
Summary: In this study, we analyzed liquid biopsy samples from metastatic breast cancer patients to study different biomarkers. We found that a higher number of circulating tumor cells was correlated with a greater likelihood of detecting clusters and megakaryocytes among the patients. The presence of megakaryocytes in the peripheral blood of cancer patients was also confirmed, which is a rare finding. Furthermore, a higher pan-inflammatory value was associated with lower overall survival, providing insight into the depth of the metastatic process. Liquid biopsy is an important source of new biomarkers for the treatment of metastatic cancer patients.
Article
Engineering, Biomedical
Stephen P. Emery, Stephanie Greene, Moataz Elsisy, Kaitlin Chung, Sang-Ho Ye, Seungil Kim, William R. Wagner, Nika Hazen, Youngjae Chun
Summary: Fetal aqueductal stenosis is a common cause of congenital hydrocephalus. Researchers have developed a new device technology for venticuloamniotic shunting to ameliorate or prevent fetal hydrocephalus development. The design and mechanical performance of the shunt device have been successfully validated through in vitro and in vivo tests.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2023)
Article
Oncology
Francois Richard, Maxim De Schepper, Marion Maetens, Sophia Leduc, Edoardo Isnaldi, Tatjana Geukens, Karen Van Baelen, Ha-Linh Nguyen, Peter Vermeulen, Steven Van Laere, Francois Bertucci, Naoto Ueno, Luc Dirix, Giuseppe Floris, Elia Biganzoli, Christine Desmedt
Summary: In this study, the genomic alterations in primary and metastatic tumor samples from patients with metastatic inflammatory breast cancer (IBC) and non-IBC were compared. Higher frequency of AURKA amplification was observed in IBC, which was associated with increased AURKA mRNA expression and worse prognosis. These findings should be further investigated considering the existence of AURKA inhibitors.
Letter
Clinical Neurology
Stephanie Greene, Jasmine L. Hect, Kristin Weaver, Michael M. McDowell
PEDIATRIC NEUROSURGERY
(2023)
Article
Biochemistry & Molecular Biology
Mokryun L. Baek, Junegoo Lee, Katherine E. Pendleton, Mariah J. Berner, Emily B. Goff, Lin Tan, Sara A. Martinez, Iqbal Mahmud, Tao Wang, Matthew D. Meyer, Bora Lim, James P. Barrish, Weston Porter, Philip L. Lorenzi, Gloria V. Echeverria
Summary: It was found that mitochondrial oxidative phosphorylation (OXPHOS) was elevated in triple negative breast cancer (TNBC) cells surviving neoadjuvant chemotherapy (NACT). DNA-damaging agents increased mitochondrial fusion and OXPHOS, while taxanes decreased these effects. Inhibiting mitochondrial fusion and fission suppressed regrowth of residual tumor cells. These findings offer potential strategies to overcome mitochondrial adaptations in chemoresistant TNBC.
Article
Oncology
Philipp R. Aldana, Ricardo A. Hanel, Joseph Piatt, Sabrina H. Han, Manisha M. Bansal, Corinna Schultz, Cynthia Gauger, John M. Pederson, John C. Wellons III, Monica L. Hulbert, Lori C. Jordan, Adnan Qureshi, Kelsey Garrity, Adam P. Robert, Asmaa Hatem, Jennifer Stein, Emily Beydler, P. David Adelson, Stephanie Greene, Paul Grabb, James Johnston, Shih-Shan Lang, Jeffrey Leonard, Suresh N. Magge, Alex Scott, Sanjay Shah, Edward R. Smith, Jodi Smith, Jennifer Strahle, Sudhakar Vadivelu, Jennifer Webb, David Wrubel
Summary: A multicenter retrospective study compared the occurrence and incidence rates of cerebrovascular events (CVEs) between a conservative management group and a surgical revascularization group in children with sickle cell disease and moyamoya syndrome (SCD-MMS). Despite more severe pretreatment disease, the surgery group had reduced odds of new CVEs after surgery and the odds of CVEs were significantly reduced after surgery compared to the presurgical period. These findings suggest that cerebral revascularization surgery, when added to conservative management, can reduce the risk of CVEs in patients with SCD-MMS.
PEDIATRIC BLOOD & CANCER
(2023)
Article
Clinical Neurology
Michael M. McDowell, Song Kim, Stephanie Greene
WORLD NEUROSURGERY
(2023)
Article
Multidisciplinary Sciences
Daniel Wang, Caroline E. Porter, Bora Lim, Amanda Rosewell Shaw, Catherine S. Robertson, Mae L. Woods, Ya Xu, Greyson G. W. Biegert, Daisuke Morita, Tao Wang, Bambi J. Grilley, Helen Heslop, Malcolm K. Brenner, Masataka Suzuki
Summary: We have demonstrated the strong antitumor activity of a binary oncolytic/helper-dependent adenovirus (CAdVEC) that lyses tumor cells, expresses IL-12 and PD-L1 blocking antibody. Following successful preclinical studies, we treated four patients with an ultralow dose of CAdVEC, which resulted in significant tumor control and immune repolarization. The combination of CAdVEC with systemic immune checkpoint antibodies induced sustained antitumor responses.
Article
Oncology
Nour Abuhadra, Ryan Sun, Clinton Yam, Gaiane M. Rauch, Qingqing Ding, Bora Lim, Alastair M. Thompson, Elizabeth A. Mittendorf, Beatriz E. Adrada, Senthil Damodaran, Kiran Virani, Jason White, Elizabeth Ravenberg, Jia Sun, Jaihee Choi, Rosalind Candelaria, Banu Arun, Naoto T. Ueno, Lumarie Santiago, Sadia Saleem, Sausan Abouharb, Rashmi K. Murthy, Nuhad Ibrahim, Aysegul Sahin, Vicente Valero, William Fraser Symmans, Jennifer K. Litton, Debu Tripathy, Stacy Moulder, Lei Huo
Summary: In this study, clinicopathologic features, including sTILs, Ki-67, PD-L1, and androgen receptor, were evaluated in 408 early-stage TNBC patients. Integrating high Ki-67 (>35%) and high sTILs (≥20%) in a computed response score model predicted a pCR rate of 65%. This model has the potential to refine patient selection for neoadjuvant clinical trials evaluating de-escalation strategies.
Article
Oncology
Evan N. Cohen, Gitanjali Jayachandran, Hui Gao, Phillip Peabody, Heather B. McBride, Franklin D. Alvarez, Megumi Kai, Juhee Song, Yu Shen, Jie S. Willey, Bora Lim, Vicente Valero, Naoto T. Ueno, James M. Reuben
Summary: Circulating tumor cells (CTCs) are valuable indicators for managing metastatic breast cancer (MBC). Enrichment of CTCs based on size and deformability can capture a wider range of tumor cells, providing a complementary approach to tissue biopsy. A longitudinal study using a microcavity array showed that the shift from epithelial CTCs to those with a mesenchymal expression pattern is associated with worse clinical outcomes.
Article
Biochemistry & Molecular Biology
Xuemei Xie, Jangsoon Lee, Jon A. Fuson, Huey Liu, Toshiaki Iwase, Kyuson Yun, Cori Margain, Debu Tripathy, Naoto T. Ueno
Summary: In this study, RNAi screening and pathway analysis identified 135 potential kinase targets whose inhibition enhanced the antiproliferation effect of eribulin in TNBC cells. The PI3K/Akt/mTOR and MAPK/JNK pathways emerged as the top candidates. Combination of eribulin with copanlisib, everolimus, trametinib, and JNK-IN-8 produced strong synergistic antiproliferative effects, with the PI3K and mTOR inhibitors showing the most potent effects in vitro.
Article
Clinical Neurology
Jasmine L. Hect, Roberta K. Sefcik, Kamil W. Nowicki, Joel Katz, Stephanie Greene
Summary: This study explores the feasibility of serial lavage for the treatment of Gram-negative rod bacterial ventriculitis. The results show that serial lavage can effectively reduce cerebrospinal fluid protein and bacterial load, and shorten the time for infection eradication.
PEDIATRIC NEUROSURGERY
(2023)
Meeting Abstract
Obstetrics & Gynecology
Anna Palatnik, Amy Y. Pan, Stephen P. Emery, Stephanie Greene, Nallammai Muthiah, Deborah R. Berman, Terry Buchmiller, Juliana S. Gebb, Jospeh Lillegard, Jena L. Miller, J. Michael M. Netwon, Patrick Schneider, Yair J. Blumenfeld, Amy Powne, Catherine Cibulskis, Kathryn Drennan, Ramesha Papanna
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2023)
Article
Oncology
Hui Chen, Qingqing Ding, Laila Khazai, Li Zhao, Senthil Damodaran, Jennifer K. Litton, Gaiane M. Rauch, Clinton Yam, Jeffrey T. Chang, Sahil Seth, Bora Lim, Alastair M. Thompson, Elizabeth A. Mittendorf, Beatriz Adrada, Kiran Virani, Jason B. White, Elizabeth Ravenberg, Xingzhi Song, Rosalind Candelaria, Banu Arun, Naoto T. Ueno, Lumarie Santiago, Sadia Saleem, Sausan Abouharb, Rashmi K. Murthy, Nuhad Ibrahim, Mark J. Routbort, Aysegul Sahin, Vicente Valero, William Fraser Symmans, Debu Tripathy, Wei-Lien Wang, Stacy Moulder, Lei Huo
Summary: This study compared the differences in PTEN expression between pre- and post-treatment tumors in patients with triple-negative breast cancer (TNBC). It also explored the possibility of using next-generation sequencing (NGS) as an alternative to immunohistochemistry (IHC) to identify PTEN loss. The results showed that testing different specimens by IHC may generate different PTEN results in a small proportion of TNBC patients.
THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY
(2023)