期刊
CHEMICAL SCIENCE
卷 6, 期 10, 页码 5435-5439出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5sc02090e
关键词
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资金
- Mid-Career Researcher Program through the NRF (National Research Foundation) of Korea - MEST [NRF-2014R1A2A1A11052980]
- Institute for Basic Science (IBS)
- MEST
A phenyl-selenium-substituted coumarin probe was synthesized for the purpose of achieving highly selective and extremely rapid detection of glutathione (GSH) over cysteine (Cys)/homocysteine (Hcy) without background fluorescence. The fluorescence intensity of the probe with GSH shows a similar to 100-fold fluorescent enhancement compared with the signal generated for other closely related amino acids, including Cys and Hcy. Importantly, the substitution reaction with the sulfhydryl group of GSH at the 4-position of the probe, which is doubly-activated by two carbonyl groups, occurs extremely fast, showing subsecond maximum fluorescence intensity attainment; equilibrium was reached within 100 ms (UV-vis). The probe selectivity for GSH was confirmed in Hep3B cells by confocal microscopy imaging.
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