Article
Agriculture, Multidisciplinary
Chen Rong-zhu, Shen Xu, Zhang Shu-ting, Zhao Hua, Chen Xiao-hui, Xu Xiao-ping, Huo Wen, Zhang Zi-hao, Lin Yu-ling, Lai Zhong-xiong
Summary: AGO proteins are essential components of the RNA-induced gene silencing complex in plants, regulating various processes from organ development to stress responses. This study identified ten AGO genes in longan, revealing their roles in different embryogenesis stages and developmental processes. Expression patterns of DlAGOs in response to hormones, light, and stress varied, providing valuable insights for understanding gene silencing mechanisms in longan.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Oncology
Yuka Ikeda, Kurumi Taniguchi, Haruka Sawamura, Sayuri Yoshikawa, Ai Tsuji, Satoru Matsuda
Summary: In this article, the invasiveness of cancer cells in relation to APRO family proteins is discussed. The role of matrix metalloproteinases (MMPs) and exosomes containing specific microRNAs in regulating cancer invasiveness is highlighted. Understanding these mechanisms can lead to innovative strategies for cancer therapy.
Article
Biochemical Research Methods
H. Robert Frost
Summary: The genetic alterations underlying cancer development are highly tissue-specific, and the biology of normal tissues can provide important information to improve cancer genomic analyses. Utilizing normal tissue data can enhance prognostic modeling, comparative analysis of different cancer types, and analysis of cancer and normal tissue pairs.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Review
Biochemical Research Methods
Tianjiao Zhang, Liang Chen, Rongzhen Li, Ning Liu, Xiaobing Huang, Garry Wong
Summary: PIWI-interacting RNAs (piRNAs) are short molecules found in various species, with the main function of monitoring and protecting the genome from transposons. They have limited bioinformatics resources compared to microRNAs (miRNAs). This review provides an overview of piRNA-specific and non-specific databases, computational models, and their role in disease associations, particularly in cancer and neurodegenerative conditions.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Biology
Pan Cheng, Xin Zhao, Lizabeth Katsnelson, Elaine M. Camacho-Hernandez, Angela Mermerian, Joseph C. Mays, Scott M. Lippman, Reyna Edith Rosales-Alvarez, Raquel Moya, Jasmine Shwetar, Dominic Grun, David Fenyo, Teresa Davoli
Summary: This study reveals the regulation of RNA and protein abundance in genes with somatic copy number alterations (SCNAs), providing insights into gene regulation. Protein complex genes are primarily regulated at the protein level, while non-complex genes are primarily regulated at the RNA level. Interestingly, genes in the same pathway show similar levels of RNA and protein regulation, with certain pathways showing stronger regulation at either the protein level or the RNA level.
Article
Oncology
Pavan Kumar Kakumani
Summary: The interplay between miRNA-AGO and multiple RBPs determines the fate of target mRNA and plays a crucial role in cancer initiation and progression.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Oncology
Monal Patel, Yinu Wang, Elizabeth T. Bartom, Rohin Dhir, Kenneth P. Nephew, Daniela Matei, Andrea E. Murmann, Ernst Lengyel, Marcus E. Peter
Summary: Research has shown that the balance of miRNAs carrying toxic and nontoxic 6mer seeds is closely related to platinum resistance in ovarian cancer. Analysis of RISC-bound miRNAs in tumors can predict treatment outcomes.
Article
Biotechnology & Applied Microbiology
Vaishnovi Sekar, Emilio Marmol-Sanchez, Panagiotis Kalogeropoulos, Laura Stanicek, Eduardo A. Sagredo, Albin Widmark, Evangelos Doukoumopoulos, Franziska Bonath, Inna Biryukova, Marc R. Friedlander
Summary: In this study, a new method called agoTRIBE is developed to comprehensively detect miRNA targets at the single-cell level. By fusing Argonaute2 protein with the RNA editing domain of ADAR2, the method allows sensitive single-cell RNA sequencing to identify miRNA targets. The results show that agoTRIBE can identify functional miRNA targets and obtain transcriptome-wide measurements of RNA abundance.
NATURE BIOTECHNOLOGY
(2023)
Article
Cell Biology
Shree Bose, Qiang Huang, Yunhan Ma, Lihua Wang, Grecia O. Rivera, Yunxin Ouyang, Regina Whitaker, Rebecca A. Gibson, Christopher D. Kontos, Andrew Berchuck, Rebecca A. Previs, Xiling Shen
Summary: Ovarian cancer cells in the omental microenvironment experience oxidative stress and adapt to this challenge through metabolic reprogramming. Inhibition of the key enzyme G6PD in the pentose phosphate pathway reduces tumor burden in OC.
Article
Microbiology
Yunjing Wang, Qian Gong, Yuyao Wu, Fan Huang, Asigul Ismayil, Danfeng Zhang, Huangai Li, Hanqing Gu, Marta Ludman, Karoly Fatyol, Yijun Qi, Keiko Yoshioka, Linda Hanley-Bowdoin, Yiguo Hong, Yule Liu
Summary: RNA interference (RNAi) is a gene regulatory and defense mechanism activated through calcium signaling, involving RNA-dependent RNA polymerase and Bifunctional nuclease to combat virus intrusion. Knockdown/knockout of Ca2+-related genes increases susceptibility to various viruses, indicating the importance of these genes in plant antiviral defense. Geminivirus V2 protein disrupts the calmodulin-CAMTA3 interaction to counteract RNAi defense, showing the versatility of host antiviral defense and viral counter-defense.
CELL HOST & MICROBE
(2021)
Article
Plant Sciences
Kai Zhao, Song Chen, Wenjing Yao, Zihan Cheng, Boru Zhou, Tingbo Jiang
Summary: The bZIP gene family plays significant roles in regulating poplar development, growth, and salt stress responses through differential gene networks or biological processes. By using systems biology and comparative genomics approaches, this study systematically explores the structures and functions of the bZIP gene family in poplar, providing the foundation for genetic breeding strategies.
Article
Microbiology
Georgina Awuah-Mensah, Jennifer McDonald, Pieter C. Steketee, Delphine Autheman, Sarah Whipple, Simon D'Archivio, Cordelia Brandt, Simon Clare, Katherine Harcourt, Gavin J. Wright, Liam J. Morrison, Catarina Gadelha, Bill Wickstead
Summary: Animal African trypanosomiasis (AAT) is a severe, wasting disease affecting domestic livestock and diverse wildlife, predominantly caused by Trypanosoma congolense and T. vivax. However, due to challenges in genetic modifications, research on the pathogenic stages is limited, hindering the understanding of these parasites' biology. The development of tools for T. congolense, including gene tagging, knockout, transgene expression, and inducible gene knockdown, will greatly aid in further research on AAT and T. congolense biology.
Article
Plant Sciences
Huai-Na Gao, Han Jiang, Xin-Yu Lian, Jian-Ying Cui, Chun-Xiang You, Yu-Jin Hao, Yuan-Yuan Li
Summary: In this study, 26 potential LTPG genes were identified in apple using bioinformatics methods, and 20 of them were confirmed as MdLTPG family members based on subcellular localization predictions. These MdLTPG genes were induced by growth conditions and one protein was shown to interact with another protein, collectively participating in wax transport during plant growth and development.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Hui Wei, Ali Movahedi, Guoyuan Liu, Yixin Li, Shiwei Liu, Chunmei Yu, Yanhong Chen, Fei Zhong, Jian Zhang
Summary: This study identified and analyzed the PtANN gene family in poplar and investigated their expression patterns under abiotic stress, revealing the molecular evolution and stress response profiles of PtANNs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Vasileios Kokkinos, Helweh Hussein, Birgit Frauscher, Mirela Simon, Alexandra Urban, Alan Bush, Anto I. Bagic, R. Mark Richardson
Summary: The study evaluated whether hippocampal spindles and barques are markers of epileptogenicity in epilepsy patients. The results show that both spindles and barques are normal entities in the hippocampal intracranial electroencephalogram and do not indicate epileptogenic properties. Understanding this is crucial for the correct interpretation of epilepsy surgery evaluations and the selection of appropriate surgical treatments.
CLINICAL NEUROPHYSIOLOGY
(2021)
Article
Physics, Applied
Christian Weisenstein, Merle Richter, Anna Katharina Wigger, Anja Bosserhoff, Peter Haring Bolivar
Summary: This article proposes an approach to use metamaterials for biomolecule detection in water by introducing substrate-integrated microfluidics. The approach allows highly sensitive detection in strongly absorbing liquids without affecting the metamaterial structure design.
APPLIED PHYSICS LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Markus V. Heppt, Anja Wessely, Eva Hornig, Claudia Kammerbauer, Saskia A. Graf, Robert Besch, Lars E. French, Alexander Matthies, Silke Kuphal, Melanie Kappelmann-Fenzl, Anja K. Bosserhoff, Carola Berking
Summary: The neural crest transcription factor BRN3A is essential for melanoma cell proliferation and survival. The mechanism underlying its aberrant expression is unknown. This study revealed that the epigenetic regulator HDAC2 plays a key role in the expression of BRN3A in melanocytes and melanoma cells. Inhibition of HDAC2 increased BRN3A expression, highlighting the importance of epigenetic mechanisms in regulating melanoma oncogenes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Judith Hauptmann, Vivien Hehne, Melanie Balzer, Lucas Bethge, Marie Wikstrom Lindholm
Summary: This study investigates the functionality of siRNAs with intentional guide strand bulges in vitro and in vivo, and demonstrates that these siRNAs can be well tolerated and effectively repress target genes.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Oncology
Isabel Stufchen, Ruth Beckervordersandforth, Stefan Fischer, Melanie Kappelmann-Fenzl, Anja Katrin Bosserhoff, Felix Beyer
Summary: The skin of adult mammals protects against various damages, with dysfunction of melanocytes and melanocyte-producing stem cells potentially leading to melanoma. The inducible Cre-loxP-system is a promising tool for studying melanocytic cells in vivo. Genes associated with neural cells are also expressed in melanocytic cells, suggesting potential for modulating melanocytic cells using specific promoters. New mouse models were presented to study and potentially modify adult melanocytic cells in vivo.
PIGMENT CELL & MELANOMA RESEARCH
(2022)
Article
Cell Biology
Lisa Laemmerhirt, Melanie Kappelmann-Fenzl, Stefan Fischer, Michaela Pommer, Tom Zimmermann, Viola Kluge, Alexander Matthies, Silke Kuphal, Anja Katrin Bosserhoff
Summary: Modifications in nuclear structures of cells play a role in diseases, including cancer. The role of nuclear lamina and related proteins in malignant melanoma is unclear. This study investigates the functional effects of dysregulated nuclear lamin B1 (LMNB1) and its nuclear receptor (LBR). It reveals their involvement in nuclear processes and their impact on senescence and chromatin state in melanoma cells. These findings suggest that LMNB1 and LBR are functionally relevant for melanoma progression.
Article
Cell Biology
Nicole Rachinger, Nora Mittag, Ines Boehme-Schaefer, Wei Xiang, Silke Kuphal, Anja K. Bosserhoff
Summary: This study reveals a novel physiological role of alpha-Synuclein (aSyn) in melanocytic morphology and its unknown function in pigment secretion in melanocytes.
Article
Chemistry, Analytical
Christian Weisenstein, Merle Richter, Anna Katharina Wigger, Anja K. Bosserhoff, Peter Haring Bolivar
Summary: THz frequency range is considered suitable for developing new bio-analytical methods due to the presence of THz-excited vibrational modes in biomacromolecules. In this study, a scalable THz metamaterial-based biosensor was used to investigate DNA samples at multiple frequencies. The results showed that the resonance frequency shift of the metamaterial depended on the presence of DNA and varied with frequency. The experiments demonstrated a significant difference in the power function between dsDNA and ssDNA, indicating an advantage for dsDNA detection and increased sensitivity at higher frequencies.
Article
Oncology
Felix Seefried, Lucia Haller, Shinji Fukuda, Aranya Thongmao, Nadja Schneider, Jochen Utikal, Shigeki Higashiyama, Anja Katrin Bosserhoff, Silke Kuphal
Summary: AREG plays multiple roles in melanoma, acting as a ligand for EGFR and an intracellular signaling molecule. Nuclear AREG appears to affect the accumulation of key proteins in the nucleus and increase resistance to therapy in melanoma cells.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Tatjana Seitz, Nora John, Judith Sommer, Peter Dietrich, Wolfgang E. Thasler, Arndt Hartmann, Katja Evert, Sven A. Lang, Anja Bosserhoff, Claus Hellerbrand
Summary: Hepatic metastasis is a critical factor for cancer-related mortality. It has been found that fibroblast growth factor 9 (FGF9) released by hepatic stellate cells (HSCs) promotes the proliferation of uveal melanoma (UM) cells, indicating FGF9 as a potential therapeutic target for hepatic metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sebastian Staebler, Adrian Lichtblau, Slavyana Gurbiel, Thomas Schubert, Alexander Riechers, Ulrike Rottensteiner-Brandl, Anja Bosserhoff
Summary: MIA/CD-RAP is a protein secreted by chondrocytes and cartilaginous tissues. MIA/CD-RAP-deficient mice develop milder osteoarthritis. This study demonstrates that MIA/CD-RAP regulates MMP13 expression and activity, suggesting its potential as a new target for osteoarthritis therapy.
Article
Cell Biology
Agnes Soederberg, Tina Meissgeier, Anja Katrin Bosserhoff, Lisa Linck-Paulus
Summary: This study reveals high expression of MAGOH and MAGOHB proteins in cutaneous melanoma cell lines and patient derived tissue samples. Knockdown of MAGOH not only inhibits melanoma cell proliferation, but also induces apoptosis. Simultaneous knockdown of MAGOH and MAGOHB enhances this effect.
Article
Oncology
Tom Zimmermann, Sebastian Staebler, R. Verena Taudte, Sumeyya Uenuevar, Sabine Groesch, Stephanie Arndt, Sigrid Karrer, Martin F. Fromm, Anja-Katrin Bosserhoff
Summary: The exact mechanisms of action of cold atmospheric plasma (CAP) treatment for cancer are still unclear. This study found that CAP treatment caused a reduction in intracellular ceramide levels and a strong activation of the unfolded protein response (UPR) in melanoma cells. Additionally, pharmacological reduction in ceramides enhanced the effects of CAP treatment.
Article
Multidisciplinary Sciences
Isabel Stuefchen, Felix Beyer, Sebastian Staebler, Stefan Fischer, Melanie Kappelmann, Ruth Beckervordersandforth, Anja K. Bosserhoff
Summary: This study investigates the role of Sox9 in the melanocytic differentiation of adult Nestin* stem cells and finds that Sox9 is crucial for determining the fate of these cells. It reveals that Sox9 acts as a fate determinant that determines whether the cells differentiate into melanocytes or glial cells.
Article
Biochemistry & Molecular Biology
Vanessa Kersten, Tatjana Seitz, Judith Sommer, Wolfgang E. E. Thasler, Anja Bosserhoff, Claus Hellerbrand
Summary: Activated hepatic stellate cells (HSCs) are involved in hepatic fibrosis and hepatocarcinogenesis. This study shows that BMP13, expressed by HSCs, promotes the progression and growth of hepatocellular carcinoma (HCC). BMP13 acts through BMP type I receptors on HCC cells and targeting this growth factor could be a potential therapeutic strategy for HCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Judith Sommer, Hanna Ehnis, Tatjana Seitz, Julia Schneider, Andreas B. Wild, Sandra Moceri, Christa Buechler, Aline Bozec, Georg F. Weber, Susanne Merkel, Ruth Beckervordersandforth, Alexander Steinkasserer, Roland Schuele, Jonel Trebicka, Arndt Hartmann, Anja Bosserhoff, Stephan von Hoersten, Peter Dietrich, Claus Hellerbrand
Summary: This study finds that obesity leads to inflammation in visceral adipose tissue, with macrophages playing a significant role. FHL2 expression is enhanced in VAT of obese individuals, and it is involved in the interaction between macrophages and adipocytes, contributing to the development of obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
