Article
Endocrinology & Metabolism
Martin Schoen, Karin Marcek Malenovska, Michal Nemec, Nikoleta Alchus Laiferova, Igor Straka, Zuzana Kosutzka, Peter Matejicka, Peter Valkovic, Jozef Ukropec, Barbara Ukropcova
Summary: This study investigated the regulation of GDF11 in cerebrospinal fluid (CSF) and blood after exercise. The results showed that running decreased the GDF11 protein content in CSF, indicating its potential role in the exercise-induced cross-talk between periphery and the brain.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Immunology
Tuula Pelkonen, Suvi Urtti, Ondina Cardoso, Irmeli Roine, Moe H. Kyaw, Heikki Peltola
Summary: The study aimed to identify clinical and CSF indices that quickly lead to the diagnosis of BM in young infants. The unclear appearance of CSF was found to be the strongest predictor of BM, emphasizing the challenges in diagnosing BM in this population.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2021)
Article
Neurosciences
Ira Driscoll, Yue Ma, Catherine L. Gallagher, Sterling C. Johnson, Sanjay Asthana, Bruce P. Hermann, Mark A. Sager, Kaj Blennow, Henrik Zetterberg, Cynthia M. Carlsson, Corinne D. Engelman, Dena B. Dubal, Ozioma C. Okonkwo
Summary: The study investigated whether carrying the advantageous KL-VS variant can attenuate age-related alterations in cerebrospinal fluid biomarkers or cognitive function in middle-aged and older adults, and found that individuals with the variant showed slower age-related changes in biomarkers and cognitive function, suggesting a protective effect against Alzheimer's disease risk.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Serafina Perrone, Simona Negro, Elisa Laschi, Marco Calderisi, Maurizio Giordano, Giuseppe De Bernardo, Gianni Parigi, Anna Laura Toni, Susanna Esposito, Giuseppe Buonocore
Summary: Metabolomics can assess and compare the metabolomic profiles of individuals born preterm and at term, showing that the urinary spectra of preterm young adults are different from those born at term and resemble the profiles at birth. The Random Forest algorithm with an accuracy of 82% identified specific metabolites responsible for the classification, indicating the potential of using urine spectra for early identification of neonates at risk of adult diseases.
Article
Acoustics
Misun Hwang, Luis Octavio Tierradentro-Garcia, Brandi L. Kozak, Kassa Darge
Summary: This study demonstrated the feasibility of using brain ultrasound (B-flow) to depict cerebrospinal fluid flow, potentially offering a new approach for the diagnosis and intervention of hydrocephalus in infants.
JOURNAL OF ULTRASOUND IN MEDICINE
(2022)
Article
Pediatrics
Andrew S. J. Marshall, Alexandra Scrivens, Jennifer L. Bell, Louise Linsell, Pollyanna Hardy, Jean Yong, Rachel Williams, Eleri Adams, Manish Sadarangani, Edmund Juszczak, Charles C. Roehr, NeoCLEAR Collaborative Group
Summary: This study found that using a sitting position instead of a lying position during lumbar punctures in newborns can improve success rates. Sitting lumbar punctures are safe, cost-neutral, and well-tolerated. These results strongly support the widespread adoption of sitting technique for neonatal lumbar punctures.
LANCET CHILD & ADOLESCENT HEALTH
(2023)
Article
Neurosciences
Takashi Tarumi, Takayuki Yamabe, Marina Fukuie, David C. Zhu, Rong Zhang, Shigehiko Ogoh, Jun Sugawara
Summary: Exercise-induced changes in cerebral blood flow (CBF) and cerebrospinal fluid (CSF) dynamics were assessed during moderate-intensity rhythmic handgrip (RHG) exercise in young healthy adults. The results demonstrated that RHG increases arterial inflow and venous outflow in the brain, while also decreasing CSF pulsatile flow. Male and female participants had distinct blood pressure responses, but similar cerebral fluid responses to RHG. The findings suggest that RHG influences both CBF and CSF dynamics to maintain intracranial volume-pressure homeostasis in young healthy adults.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Veterinary Sciences
Amy B. Yanke, Kendall E. Day, Amanda R. Taylor, Crisanta Cruz-Espindola, Dawn M. Boothe
Summary: The study aimed to determine the oral dose of mebendazole (an anti-parasitic drug) required to achieve concentrations ≥10 ng/mL in the cerebrospinal fluid (CSF) of healthy dogs. The results showed that both doses of 100 mg/kg and 200 mg/kg of mebendazole orally could reach this concentration, but the 200 mg/kg dose achieved more consistent concentrations.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Siwei Chen, Justine Lee, Tri Minh Truong, Sammy Alhassen, Pierre Baldi, Amal Alachkar
Summary: This study identified distinct metabolic profiles in neonatal and adult mouse brains, with lipid metabolites showing the most significant changes. Some lipid species exhibited up to 1000-fold changes. Additionally, age-dependent increases and decreases were observed among lipids and non-lipid metabolites, respectively.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Medicine, General & Internal
Massimiliano Castellazzi, Caterina Ferri, Sarah Alfiero, Ilenia Lombardo, Michele Laudisi, Ginevra Tecilla, Michela Boni, Stefano Pizzicotti, Enrico Fainardi, Tiziana Bellini, Maura Pugliatti
Summary: The study found that male neurological patients have higher levels of CSF serum-derived proteins, such as albumin and IgG, compared to females. This difference showed varying degrees of agreement between different genders and were positively correlated with age.
Article
Microbiology
Yongyong Kang, Xinchao Ji, Li Guo, Han Xia, Xiaofei Yang, Zhen Xie, Xiaodan Shi, Rui Wu, Dongyun Feng, Chen Wang, Min Chen, Wenliang Zhang, Hong Wei, Yuanlin Guan, Kai Ye, Gang Zhao
Summary: Metagenomic and metatranscriptomic sequencing of cerebrospinal fluid (CSF) collected from 23 pregnant women did not find evidence of a colonized microbial community in the CSF of healthy individuals. This finding enhances our understanding of the internal environment of CSF in healthy people, with implications for human health, particularly in neurological infections and disorders.
MICROBIOLOGY SPECTRUM
(2021)
Article
Medicine, General & Internal
Virginia Pomar, Natividad Benito, Pol Duch, Marc Colome, Alba Rivera, Pere Domingo
Summary: A long-term observational study of adult BM patients in Barcelona has found that in recent years, patients without underlying conditions who develop BM are older and more likely to have pneumococcal or listerial infections and systemic complications, although the in-hospital mortality has decreased compared to the first period.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Immunology
Nina Vindegaard Sorensen, Nis Borbye-Lorenzen, Rune Haubo Bojesen Christensen, Sonja Orlovska-Waast, Rose Jeppesen, Kristin Skogstrand, Michael Eriksen Benros
Summary: By analyzing the cytokine levels in the cerebrospinal fluid (CSF) of patients with recent-onset depression and healthy controls, this study found that IL-4, MCP-1, and MIP-1 beta levels were significantly higher in patients with depression compared to healthy controls, while IL-6 and IL-8 levels showed no significant differences.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Public, Environmental & Occupational Health
Juan M. Lopez, Vanessa E. Leyva, Javier A. Bustos, Erika Perez, Sofia S. Sanchez, Herbert Saavedra, Isidro Gonzales, Helena Maruenda, Hector H. Garcia, Cysticercosis Working Grp Peru
Summary: The diagnosis of neurocysticercosis (NCC) is primarily based on neuroimaging, but this method is rarely available in rural areas. Immunological assays can complement neuroimaging, but field-applicable assays are currently unavailable. In an exploratory study, urine samples were analyzed using H-1-nuclear magnetic resonance-based metabolomics to distinguish NCC patients from healthy control subjects. The results suggest the potential of using metabolomic biomarkers for detecting NCC.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Veterinary Sciences
Mohammed A. H. Abdelhakiem, Hussein Awad Hussein
Summary: Diseases of the central nervous system can cause morbidity and mortality in equine. Collection and analysis of cerebrospinal fluid provide valuable information on degenerative and inflammatory diseases. This study aimed to assess clinical complications of CSF collection in healthy donkeys and establish reference values for cytological and biochemical parameters.
BMC VETERINARY RESEARCH
(2021)
Article
Food Science & Technology
Federica Barbieri, Luca Laghi, Chiara Montanari, Qiuyu Lan, Alessia Levante, Fausto Gardini, Giulia Tabanelli
Summary: In this study, a metabolomic approach was used to compare five different strains of Latilactobacillus sakei. The results showed that the metabolic diversity of L. sakei is related to its functionality in fermented sausages, and nuclear magnetic resonance (H-1-NMR) is an effective method for the rapid identification of different classes of compounds.
Article
Food Science & Technology
Silvia Tappi, Elena Velickova, Cinzia Mannozzi, Urszula Tylewicz, Luca Laghi, Pietro Rocculi
Summary: This study evaluated the effect of different solutions for vacuum impregnation of fresh-cut apples through various analytical methods. The results showed good preservation of color and higher firmness in the impregnated samples. TD-NMR studies revealed changes in signal intensity and T2 relaxation time after storage, indicating the dynamic nature of the plant material.
Article
Biotechnology & Applied Microbiology
Lorenzo Nissen, Camilla Aniballi, Flavia Casciano, Alberto Elmi, Domenico Ventrella, Augusta Zannoni, Andrea Gianotti, Maria Laura Bacci
Summary: The first weeks of life are crucial for the microbial colonization of piglets' gastrointestinal tract. This study used an in vitro model to evaluate the impact of different concentrations of amoxicillin in milk on the piglets' colon microbiota. The results showed that amoxicillin had an influence on the microbiota and metabolites production, but did not alter the overall biodiversity. There was an increase in resistant species.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
E. Fiore, A. Lisuzzo, L. Laghi, K. J. Harvatine, E. Mazzotta, M. C. Alterisio, P. Ciaramella, C. Zhu, B. Contiero, V. Faillace, J. Guccione
Summary: The metabolic profile of Mediterranean buffaloes (MB) associated with different levels of beta-hydroxybutyrate (BHB) was characterized. The study revealed metabolic changes related to subclinical ketosis in MB, suggesting the need for further investigation to better understand this disease.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Food Science & Technology
Chenglin Zhu, Zhibo Yang, Xuan Lu, Yuwen Yi, Qing Tian, Jing Deng, Dan Jiang, Junni Tang, Luca Laghi
Summary: In recent years, there has been a significant increase in the interest for pear cider as a means to utilize fruits that are unsuitable for direct consumption or to promote pear varieties grown in restricted regions. This study analyzed the taste characteristics and metabolomic profiles of pear cider produced from China's Guangan honey pear by using four different commercial Saccharomyces cerevisiae strains. The results showed that different yeast strains led to distinct changes in metabolic pathways during fermentation, and the study provided valuable insights for improving the quality of pear cider and its large-scale production.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Chenglin Zhu, Qian Zhang, Xin Zhao, Zhibo Yang, Falong Yang, Yang Yang, Junni Tang, Luca Laghi
Summary: This study aimed to investigate mastitis in dairy cows by comparing multiple biological specimens from individuals with or without clinical mastitis, using untargeted H-1-NMR. Integrated analysis of metabolome changes across key biofluids revealed several pathways related to energy and amino acid metabolism affected by clinical mastitis. This work provides valuable insights for the development of strategies to improve the metabolic status of mastitic dairy cows.
Article
Biochemistry & Molecular Biology
Fulvio Laus, Luca Laghi, Marilena Bazzano, Maria Grazia Cifone, Benedetta Cinque, Yaosen Yang, Andrea Marchegiani
Summary: Positive effects on animal health, feed efficiency, and milk's nutritional content have been found after oral administration of probiotics. This study aimed to investigate the impact of dietary supplementation with high numbers of multispecies probiotic formulations on milk metabolomic profiles in donkeys. The results showed changes in metabolite concentrations and increased activity of alk-SMase and ALP after 30 days of probiotic supplementation.
Article
Food Science & Technology
Qian Zhang, Jinhua Cheng, Xiaole Jiang, Junni Tang, Chenglin Zhu, Hong Chen, Luca Laghi
Summary: The aim of this study was to investigate the impact of different fibers on the metabolome of cecum content in obese mice. The results showed that a high-fat diet increased acetone and methionine levels while decreasing methanol, arabinose, acetate, and 3-hydroxyphenylacetate levels in the cecum contents. Supplementation with mixed fibers resulted in higher levels of fumarate and hypoxanthine and lower levels of phenylacetate, acetate, fucose, formate, proline, betaine, and TMAO compared to the high-fat diet.
Article
Food Science & Technology
Xin Zhao, Jianying Feng, Luca Laghi, Jing Deng, Xiaofang Dao, Junni Tang, Lili Ji, Chenglin Zhu, Gianfranco Picone
Summary: The flavor profiles of sour meat from goose and pork were evaluated using gas chromatography-ion mobility spectrometry (GC-IMS) combined with an electronic nose (E-nose) and tongue (E-tongue). The source of the raw meat plays a crucial role in the formation of flavor compounds during the fermentation process. A robust principal component model (RPCA) could effectively differentiate sour meat from the two sources based on the odor and taste response values obtained by the E-nose and E-tongue.
Article
Food Science & Technology
Qian Zhang, Jian Ma, Yupei Yang, Jing Deng, Kaixian Zhu, Yuwen Yi, Junni Tang, Xiaole Jiang, Chenglin Zhu, Luca Laghi
Summary: Fermenting kiwifruit into kiwi wine can reduce economic losses, and this study evaluated the sensory characteristics and flavor profiles of kiwi wine fermented by different yeast strains, finding suitable strains for kiwi wine production.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Chiara Bernardini, Debora La Mantia, Roberta Salaroli, Domenico Ventrella, Alberto Elmi, Augusta Zannoni, Monica Forni
Summary: It has been demonstrated that blood vessels contain multipotent progenitor cells, including mesenchymal stem cells. In this study, a new improved method was described to isolate a pure population of vascular wall cells from the thoracic aorta of Gottingen Minipigs, while also collecting endothelial cells. The results showed that this new protocol allows for the isolation of a pure population of vascular wall mesenchymal stem cells (VW-MSCs) and endothelial cells, opening possibilities for in vitro vascular remodeling studies.
Article
Biochemistry & Molecular Biology
Fulvio Laus, Rodolfo Gialletti, Marilena Bazzano, Luca Laghi, Fabrizio Dini, Andrea Marchegiani
Summary: This study used metabolomic analysis to compare the composition of synovial fluid between healthy and OA-affected joints in horses. The results showed significant differences in the concentration of various metabolites in the synovial fluid of OA horses compared to healthy horses. These findings reveal the complex mechanism of osteoarthritis and demonstrate the value of metabolomics in clinical research.
Article
Cardiac & Cardiovascular Systems
Anna Lorenzin, Luca Sgarabotto, Maria Laura Bacci, Alberto Elmi, Domenico Ventrella, Camilla Aniballi, Monica Zanella, Alessandra Brendolan, Luca Di Lullo, Claudio Ronco
Summary: This study presents a new miniaturized device called AD1 for extracorporeal ultrafiltration, designed for patients with fluid overload. The device operates at low pressure and flow regimes and has a user-friendly interface. The in vivo tests showed that it is safe, reliable, and accurate, opening up possibilities for clinical trials in various settings.
CARDIORENAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Chenglin Zhu, Xin Pan, Guo Li, Caiwu Li, Daifu Wu, Junni Tang, Yan Huang, Likou Zou, Luca Laghi
Summary: Lipidic metabolites play essential roles in the physiological health and growth performance of giant pandas. Bamboo, as their main diet, determines the lipidome of giant pandas. This study explores the lipidomic atlas of different parts of bamboo and investigates the changes in fecal and serum lipidome during the consumption of bamboo shoots, which is typical in spring. The findings suggest the importance of a balanced diet for giant panda health, even in the case of a single food-based diet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
