Article
Biophysics
Felix Hempel, Jessica Ka Yan Law, Thanh Chien Nguyen, Ruben Lanche, Anna Susloparova, Xuan Thang Vu, Sven Ingebrandt
Summary: The study investigated single-cell adhesion on organic electrochemical transistors using an impedimetric readout method, demonstrating single-cell resolution and potential future applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Ning Yang, Wenhao Hui, Sizhe Dong, Xuming Zhang, Liyang Shao, Yanwei Jia, Pui-In Mak, Rui Paulo da Silva Martins
Summary: A method for joint assessment of cell viability and vitality based on ECIS technology and environmental temperature control was proposed, providing accurate evaluation of cell health status. Compared to traditional methods, this approach is simpler, faster, and less dependent on sample conditions.
Article
Nanoscience & Nanotechnology
Yu-Sheng Hsiao, Edgar Daniel Quinones, Shih-Chieh Yen, Jiashing Yu, Ji-Tseng Fang, Peilin Chen, Ruey-Shin Juang
Summary: Electric cell-substrate impedance sensing (ECIS) is an innovative approach for the label-free and real-time detection of cell morphology, growth, and apoptosis. In this study, poly(3,4-ethylenedioxythiophene)-poly-(styrenesulfonate) (PEDOT:PSS) conducting polymer (CP)-based bioelectrodes were developed for the simultaneous drug release and real-time biosensing of cancer cell viability. This device can reliably monitor impedance signal changes correlated with cell viability while exhibiting excellent properties for both drug encapsulation and on-demand release.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Yitao Liang, Lin Ji, Tingting Tu, Shanshan Zhang, Bo Liang, Xuesong Ye
Summary: This study developed an on-site platform to monitor the invasion process of cancer cells, optimized parameters to demonstrate experimental results, indicating that changes in impedance magnitude reflect interactions between cells and the extracellular matrix. Further use of this platform could contribute to research on cell metastasis.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Cell Biology
Ineke D. C. Jansen, Thijs van Velzen, Teun J. de Vries, Robert Szulcek, Jack J. W. A. van Loon
Summary: In this study, an impedimetric bioassay was introduced for real-time monitoring of osteoclast resorption. The method involves culturing osteoclasts on a calcium phosphate substrate with electrodes and applying different chemical stimuli to measure their activity continuously. The results showed that inflammatory factors and bisphosphonates significantly affected osteoclastic activity. This method is of great importance for studying osteoclast function and diseases, as well as for high-throughput drug testing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Monika Prendecka-Wrobel, Dominika Pigon-Zajac, Magdalena Jaszek, Anna Matuszewska, Dawid Stefaniuk, Grzegorz Opielak, Katarzyna Piotrowska, Mansur Rahnama-Hezavah, Teresa Malecka-Massalska
Summary: This study investigated the pharmacokinetics of an active substance obtained from the medicinal fungus Cerrena unicolor at different concentrations. The results showed that the substance had no significant effects on normal cells, but exhibited inhibitory effects on colon cancer cells.
Review
Engineering, Electrical & Electronic
Ioana Voiculescu, Fang Li, Anis Nurashikin Nordin
Summary: The ECIS technique is a powerful and effective tool for real-time monitoring of cell behavior, with applications ranging from impedance of cancer cells and toxicity studies to analysis of beating cardiomyocytes. It offers advantages over conventional assays and provides opportunities for innovative research in the field of mammalian cell properties and physiological functions.
IEEE SENSORS JOURNAL
(2021)
Article
Chemistry, Analytical
Anna Ronja Dorothea Binder, Andrej-Nikolai Spiess, Michael W. Pfaffl
Summary: Measurement of cell surface coverage using electric cell-substrate impedance sensing (ECIS) is a common technique for assessing cell growth behavior. A large toolset of R scripts was developed to transform and quantify ECIS growth curves in different experimental settings, allowing extraction of quantitative data for analysis. The scripts were tested in real-life cell culture experiments and calibration setups, providing comparability and reliable quantification.
Article
Biochemistry & Molecular Biology
Nadja Engel, Michael Dau, Vivien Engel, Denise Franz, Fabian Klemmstein, Christiane Thanisch, Juergen F. Kolb, Marcus Frank, Armin Springer, Ruediger Koehling, Rainer Bader, Bernhard Frerich, Nadine Wiesmann, Diana Heimes, Peer W. Kaemmerer
Summary: By combining electrical stimulation of a commercial titanium implant with real-time impedance sensing, osteogenesis progress can be monitored and electrical stimulation can be adjusted accordingly for individual fracture healing.
Article
Chemistry, Analytical
Chen Zhou, Sebastian Bette, Aaron Babendreyer, Christina Hoffmann, Sven Gerlach, Tom Kremers, Andreas Ludwig, Bernd Hoffmann, Rudolf Merkel, Stefan Uhlig, Uwe Schnakenberg
Summary: The development of a cell-stretching platform utilizing stretchable microelectrodes and an ultra-elastic substrate allows for up to 35% cyclic stretch. The novel interlaced meander design ensures reliable electrical contacting of the microelectrodes under stretch, enabling label-free, real-time monitoring of cell monolayers. Proof-of-concept experiments show that the system can discern impedance changes in different epithelial cell types, providing a potential tool for continuous monitoring of cell monolayers under strain for various applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Zheyuan Zhang, Xichen Yuan, Huijie Guo, Peng Shang
Summary: Detection sensitivity is crucial in ECIS sensor application, and electrode configuration directly affects sensitivity. This study fabricated ECIS sensor arrays with various electrode configurations to monitor living MC3T3-E1 cells. The results showed that smaller and fewer electrodes with a diameter larger than 25 μm had higher impedance values and generated more impedance shift to cell status change. However, when the electrode diameter was smaller, there was no clear relationship between impedance and membrane capacitance shift to cell status change and electrode geometry. The proposed sensor chip provides a basis for selecting electrode configuration of monopolar electrodes and enables sustained and stable detection of cellular impedance.
Article
Biochemistry & Molecular Biology
Chien-Chu Huang, Shao-Chih Chiu, Shih-Chi Chao, Heng-Yi Liao, Shiao-Pieng Lee, Chun-Chung Huang, Der-Yang Cho
Summary: CBD has a cytotoxic effect on oral cancer cells and induces apoptosis. It also affects the morphology and migration of OEC-M1 cells. ECIS analysis shows that CBD decreases the overall resistance and morphological parameters of the cells in a concentration-dependent manner. The study demonstrates that ECIS is more sensitive, functional, and comprehensive than traditional biochemical assays.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Fuentes-Velez Susana, Fagoonee Sharmila, Sanginario Alessandro, Gallo Valentina, Riganti Chiara, Pizzi Marco, Altruda Fiorella, Demarchi Danilo
Summary: Interest in impedance-based cellular assays is growing due to their multiple advantages, and our study demonstrates the potential of ECIS in cancer treatment decisions and early detection of chemoresistance. The analysis of impedance-based curves distinguishes between chemo-sensitive and chemo-resistant cells, and promises the study of time-dependent factors related to resistance acquisition in cells. The continuous monitoring and highthroughput early detection of drug resistance using the ECIS device presents new possibilities for cancer treatment and drug screening.
Article
Chemistry, Analytical
Remi Bettenfeld, Julien Claudel, Djilali Kourtiche, Mustapha Nadi, Cyril Schlauder
Summary: In this paper, a lab-on-a-chip device is developed for bioimpedance spectroscopy and electric field exposure of single cells. The device includes a microfluidic cell-trapping system and microelectrodes for electrical characterization. The first test confirms the suitability of the device for studying cell membrane electropermeabilization.
Article
Chemistry, Analytical
Eiichiro Yamaguchi, Joshua Yao, Allison Aymond, Douglas B. Chrisey, Gary F. Nieman, Jason H. T. Bates, Donald P. Gaver
Summary: This study investigates the susceptibility and disruption of barrier function in epithelial cells during acute respiratory distress syndrome and ventilator-induced lung injury. The results show that cells with higher barrier function have a better ability to resist lung injury, but the barrier function eventually weakens and the cell layer disintegrates. The study also finds that intercellular binding is the first line of defense against lung injury.