Article
Polymer Science
Huynh-Quang-Dieu Nguyen, Chen-Yu Kao, Chien-Ping Chiang, Yu-Han Hung, Chun-Min Lo
Summary: This study investigates the immunomodulatory properties of extracellular matrix (ECM) and mesenchymal stem cells (MSCs). The results show that degradation products from porcine bladder ECM can reduce inflammation and increase the production of anti-inflammatory cytokines from macrophages. MSCs cultured on the ECM hydrogel demonstrate immunomodulatory properties at both gene and protein levels. The findings provide a basis for further research on immunomodulation therapy.
Article
Biochemistry & Molecular Biology
Francesca Diomede, Luigia Fonticoli, Guya Diletta Marconi, Ylenia Della Rocca, Thangavelu Soundara Rajan, Oriana Trubiani, Giovanna Murmura, Jacopo Pizzicannella
Summary: The study aimed to develop an in vitro culture system using decellularized dental pulp (DDP) matrix, 5-Aza-2'-deoxycytidine (5-Aza), and extracellular vesicles (EVs) derived from human dental pulp stem cells (hDPSCs) to facilitate dental pulp regeneration.
Article
Materials Science, Biomaterials
Xinxin Li, Zhaoxia Yu, Shihui Jiang, Xiaohua Dai, Guanhua Wang, Yue Wang, Zhimou Yang, Jie Gao, Huiru Zou
Summary: This study constructed amelogenin self-assembling peptide hydrogels and investigated their effects on the odontogenic differentiation of human dental pulp cells (HDPCs). The results showed that the D-gel exhibited the strongest enhancement effects and induced odontogenic differentiation through the MAPK-ERK1/2 and transforming growth factor/smad pathways. This study demonstrated the potential of amelogenin peptide hydrogel in dentine-pulp complex regeneration.
REGENERATIVE BIOMATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Yuanyuan Shi, Yingxin Wang, Zhaochen Shan, Zhenhua Gao
Summary: Decellularized submandibular gland extracellular matrix (DSMG) was found to have similar structure and composition to dental pulp. DSMG could serve as an alternative scaffold for dental pulp regeneration, supporting adhesion and proliferation of dental pulp stem cells. Implanted cell-seeded DSMG formed a vascularized dental pulp-like tissue expressing markers involved in dentinogenesis and angiogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Can Ergun, Mahmut Parmaksiz, Murat Taner Vurat, Ayse Eser Elcin, Yasar Murat Elcin
Summary: A macroporous 3D scaffold derived from decellularized bovine liver ECM was developed with defined biological, physical, and mechanical properties. The scaffold showed stable mechanical performance and high hemocompatibility in vitro. Furthermore, it was demonstrated that the scaffold is suitable for use as a cell substrate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Liwen Zheng, Yaxian Liu, Lin Jiang, Xiaoping Wang, Yuqin Chen, Lan Li, Mingyu Song, Hongmei Zhang, Yu Shrike Zhang, Ximu Zhang
Summary: This study proposes a new strategy for pulp regeneration by using hydrogel microspheres incorporated with decellularized dental pulp matrix-derived bioactive factors to simulate a pulp-specific three-dimensional microenvironment. The constructed microspheres exhibit favorable plasticity, biocompatibility, and biological performances, and hDPSCs cultured on the microspheres demonstrate enhanced pulp-formation ability. In vivo experiments show that the microspheres facilitate the regeneration of pulp-like tissue and new dentin. The decellularized pulp matrix-derived bioactive factors mediate the multi-directional differentiation of hDPSCs to regenerate the pulp tissue.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Alexey A. Ivanov, Tamara I. Danilova, Alla V. Kuznetsova, Olga P. Popova, Oleg O. Yanushevich
Summary: The regeneration of periodontal tissues is important for the treatment of periodontitis. This study investigated the osteogenic and odontogenic differentiation of periodontal progenitor and stem cells in decellularized tooth matrix (dTM) and periodontal ligament (dPDL) in both 2D and 3D culture conditions. The results showed that the combination of dTM and dPDL promoted both osteogenic and odontogenic differentiation of periodontal cells.
Review
Chemistry, Physical
Necdet Adanir, Zohaib Khurshid, Jithendra Ratnayake
Summary: This systematic review evaluates the effectiveness of decellularized dental pulp extracellular matrix (DP-ECM) in pulpal regeneration. Although the results of the studies suggest that DP-ECM may stimulate pulpal regeneration, the presence of bias and methodological deficiencies limit the ability to draw definitive conclusions. Further research, both clinical and pre-clinical, is needed to provide more conclusive evidence.
Article
Biotechnology & Applied Microbiology
Jiahui Fu, Jianfeng Chen, Wenjun Li, Xiaomin Yang, Jingyan Yang, Huixin Quan, Haitao Huang, Gang Chen
Summary: The supplementation of laminins to DPEM enhances odontogenic differentiation of cells and contributes to the formation of the odontoblast layer in dental pulp regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jing Cui, Yan-Jing Zhang, Xuan Li, Jia-Jiao Luo, Lei-Lei Zhao, Xin-Yue Xie, Wei Ding, Jing-Cong Luo, Ting-Wu Qin
Summary: Extracellular vesicles derived from tendon-derived stem cells were bound to collagen using a collagen binding domain, resulting in the fabrication of a bio-functionalized scaffold. The scaffold promoted stem cell proliferation, migration, and tenogenic differentiation in vitro, and also facilitated stem cell recruitment, collagen deposition, and biomechanical improvement in injured tendons, leading to functional tendon regeneration.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Rui Zhang, Junqun Jiang, Yaling Yu, Fangfang Wang, Niuniu Gao, Yingjie Zhou, Xinlong Wan, Zhibin Wang, Peng Wei, Jin Mei
Summary: Chronic kidney disease is a major global public health problem, with renal fibrosis being a common pathological process in chronic renal failure. Research indicates that renal fibrosis decellularized scaffolds have the potential to serve as a model for cellular mechanisms of tissue fibrosis or donors for tissue engineering. Analysis through histology, proteomics, and ELISA showed changes in the structure and components of fibrotic tissue. The fibrosis model induced by adenine-fed demonstrated changes in the fibrotic scaffold's structure and protein expression, but cytokines associated with renal regeneration post-injury were maintained, indicating potential for tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Nunthawan Nowwarote, Stephane Petit, Francois Come Ferre, Florent Dingli, Victor Laigle, Damarys Loew, Thanaphum Osathanon, Benjamin P. J. Fournier
Summary: The study identified the matrisome of ECM proteins derived from human DPSCs and found that it can regulate mineralization, even in cells which do not respond to mineralization assaults like GF.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Endocrinology & Metabolism
Silke De Vriendt, Celia Mesias Casares, Susana Rocha, Hugo Vankelecom
Summary: The uterus-lining endometrium is crucial for mammalian reproduction, but its mechanisms and diseases are not well understood due to its hidden location and lack of suitable research models. Recently, 3D organoid models have been developed to replicate the endometrium, including the reproduction of the menstrual cycle. However, there are limitations in the current use of organoids, such as lack of standardization and xenografting, which hinder clinical translation. Therefore, researchers are seeking better alternatives, such as fully defined matrices, to improve the growth of organoids and advance their application in basic research and clinical fields.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Engineering, Biomedical
Yonggang Lv, Hongjun Wang, Gui Li, Boyuan Zhao
Summary: A study prepared 3D DECM scaffolds with suitable stiffness to mimic the tumor microenvironment of human breast tissue, exploring the impact of matrix stiffness on drug resistance in breast cancer cells.
BIOACTIVE MATERIALS
(2021)
Article
Biology
Nerea Garcia-Urkia, Jon Luzuriaga, Veronica Uribe-Etxebarria, Igor Irastorza, Francisco Javier Fernandez-San-Argimiro, Beatriz Olalde, Nerea Briz, Fernando Unda, Gaskon Ibarretxe, Iratxe Madarieta, Jose Ramon Pineda
Summary: Obesity has a negative impact on human lifespan and is a significant public health issue worldwide. However, the development of in vitro 3D adipose tissue models is limited and expensive. In this study, we designed and produced 3D solid foams using a mixture of collagen and decellularized adipose tissue to mimic the in vivo adipose microenvironment. We demonstrated that these foams are compatible with dental pulp stem cell culture and can support adipocyte differentiation.
Article
Engineering, Biomedical
Maria-Costanza Chiti, Julie Vanacker, Emna Ouni, Natalija Tatic, Aiswarya Viswanath, Anne des Rieux, Marie-Madeleine Dolmans, Lisa Jane White, Christiani Andrade Amorim
Summary: The study developed an oECM hydrogel derived from decellularized bovine ovaries, capable of supporting human ovarian follicle survival and growth in vitro when combined with alginate. Increasing alginate content in the hydrogel improved follicle recovery rates, but did not significantly impact follicle viability or growth.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Marco Tatullo, Maria Giovanna Gandolfi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Marco Tatullo, Adriano Piattelli, Barbara Zavan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Francesco Paduano, Emma Colao, Fernanda Fabiani, Valentina Rocca, Francesca Dinatolo, Adele Dattola, Lucia D'Antona, Rosario Amato, Francesco Trapasso, Francesco Baudi, Nicola Perrotti, Rodolfo Iuliano
Summary: The study revealed that germline pathogenic variants accounted for a certain proportion in some patients, and multi-gene panel testing can be an effective diagnostic approach for individuals at high risk of hereditary cancers.
Review
Biochemistry & Molecular Biology
Mariacristina Amato, Simona Santonocito, Gaia Viglianisi, Marco Tatullo, Gaetano Isola
Summary: Periodontal disease is a common chronic inflammatory condition that lacks effective treatment options. Utilizing stem cells for periodontal tissue regeneration shows promise as a potential therapeutic approach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Francesco Paduano, Eugenio Gaudio, Francesco Trapasso
Summary: Fhit protein is often reduced in human tumors, and its restoration can trigger apoptosis of cancer cells and suppress tumor formation. In this study, Fhit expression was found to be significantly reduced in human melanoma cells, and the use of a recombinant adenovirus carrying the FHIT gene blocked their in vivo growth. It was also discovered that Fhit physically interacts with Hsp90, a chaperone that plays a crucial role in the conformational maturation and stabilization of C-Raf. Furthermore, the combination of Hsp90 inhibitor 17-AAG and Fhit protein overexpression in melanoma cells led to increased C-Raf ubiquitination and degradation.
Article
Engineering, Biomedical
Simon C. Kellaway, Victoria Roberton, Joshua N. Jones, Rabea Loczenski, James B. Phillips, Lisa J. White
Summary: Incorporating dECMh derived from bone, liver, and small intestinal tissues can promote neurite extension and axonal regeneration, providing an alternative to current collagen I hydrogel.
ACTA BIOMATERIALIA
(2023)
Article
Anatomy & Morphology
Priscilla Pelaez-Cruz, Pia Lopez Jornet, Marco Tatullo, Eduardo Pons-Fuster Lopez
Summary: This study evaluated the in vitro effect of bovine, porcine, and beta-tricalcium phosphate bone substitutes, and their combination with polyphenol epigallocatechin-3-gallate (EGCG), on cultured dental pulp stem cells (DPSCs). The addition of EGCG reversed the cytotoxic effect of certain biomaterials and improved cell adhesion, migration, and alkaline phosphatase activity. EGCG shows potential as a strategy for enhancing the biocompatibility and osteoconductive properties of bone substitutes in bone regeneration treatments.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2023)
Article
Biochemistry & Molecular Biology
Bruno Lamanna, Marina Vinciguerra, Miriam Dellino, Gabriele Cascella, Gerardo Cazzato, Enrica Macorano, Antonio Malvasi, Salvatore Scacco, Ettore Cicinelli, Vera Loizzi, Antonella Vimercati, Gennaro Cormio, Francesco Paduano, Eliano Cascardi, Marco Tatullo
Summary: Turner syndrome is a chromosomal disorder characterized by short stature and infertility. It can be accompanied by Duchenne muscular dystrophy, a neuromuscular disease that primarily affects males.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Marco Tatullo, Barbara Zavan, Adriano Piattelli
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Medicine, General & Internal
Marco Tatullo, Sandro Rengo, Gilberto Sammartino, Gaetano Marenzi
JOURNAL OF CLINICAL MEDICINE
(2023)
Editorial Material
Chemistry, Physical
Marco Tatullo, Giuseppina Ambrogio, Gilberto Sammartino
Article
Oncology
Eliano Cascardi, Gerardo Cazzato, Giuseppe Ingravallo, Miriam Dellino, Carmelo Lupo, Nadia Casatta, Andrea Ballini, Andrea Pacifici, Gaetano Marenzi, Gilberto Sammartino, Eugenio Maiorano, Marco Tatullo
Summary: Oral malignant melanoma (OMM), accounting for less than 1% of all melanomas, commonly develops on the oral mucosa and often faces a diagnostic delay. This study explored the use of PRAME as a tool to investigate and differentiate OMM from other dysplastic pigmented lesions. The findings showed that PRAME could accurately differentiate Melanoma cases from other melanocytic nevi, leading to better differential diagnosis, early diagnosis, and clinical/surgical management of oncological lesions.
Review
Cell & Tissue Engineering
Marco Tatullo
Summary: Aging is characterized by physiological changes that increase susceptibility to age-related diseases. This review summarizes the alterations affecting MSCs during aging and the role of inflammation in age-related syndromes.