Article
Biotechnology & Applied Microbiology
Guxin Cui, Pengpeng Li, Ruixue Wu, Huancai Lin
Summary: The study found that membrane vesicles (MVs) of Streptococcus mutans can inhibit biofilm formation of Streptococcus gordonii and Streptococcus sanguinis, revealing the important role of MVs in interspecies interactions.
Article
Biochemistry & Molecular Biology
Eduardo M. Franco, Livia A. Alves, Hassan Naveed, Victor A. A. Freitas, Debora C. Bastos, Renata O. Mattos-Graner
Summary: Bacterial surface proteins assembled into amyloids play important roles in biofilm formation and host immune evasion in Streptococcus sanguinis. The study demonstrates the significance of sortase A in amyloidogenesis and reveals the correlation between amyloid production and biofilm formation as well as immune evasion phenotypes in different strains of S. sanguinis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Yi-Ywan M. Chen, Hsing-Yi Wang, Chia-Hua Wu, Yu-Juan Lin, Cheng-Hsun Chiu
Summary: Although gene clusters encoding Tfp are commonly found in Streptococcus sanguinis, not all strains exhibit surface-dependent twitching motility. Tfp can serve as a ligand for host cell colonization, and Tfp retraction is essential for biofilm stability. The twitching phenotype is strain-specific and sensitive to growth media.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Giuseppe Pezzotti, Satomi Ofuji, Hayata Imamura, Tetsuya Adachi, Toshiro Yamamoto, Narisato Kanamura, Eriko Ohgitani, Elia Marin, Wenliang Zhu, Osam Mazda, Azusa Togo, Satoshi Kimura, Tadahisa Iwata, Hideki Shiba, Kazuhisa Ouhara, Takashi Aoki, Toshihisa Kawai
Summary: This study investigated the mechanisms of competition/coexistence between Streptococcus sanguinis and Streptococcus mutans using quantitative Raman spectroscopy and imaging. The results suggest that the two species engage in antagonistic interactions and that Streptococcus sanguinis acts to disrupt the biofilm constructed by Streptococcus mutans. The main chemical weapon used by Streptococcus sanguinis in coculture is the secretion of oxygen radicals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Ulrike Redanz, Sylvio Redanz, Puthalayai Treerat, Sivaraman Prakasam, Li-Jung Lin, Justin Merritt, Jens Kreth
Summary: This study investigates the transition of polymicrobial communities in oral health from a homeostatic to a dysbiotic state using a multispecies biofilm model. The research highlights the host-microbiome interactions during this transition and demonstrates the variability of host cell responses between different cell lines, emphasizing the importance of relevant in vitro models in studying oral microbiome-host interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Xiaoying Lyu, Liang Wang, Yusen Shui, Qingsong Jiang, Lan Chen, Wen Yang, Xiaoya He, Jumei Zeng, Yuqing Li
Summary: The study found that UA exhibited significant antimicrobial activity against common oral streptococci, reduced EPS synthesis of oral streptococci, and suppressed gtf genes' expression. Furthermore, UA decreased the proportion of S. mutans in multi-species biofilms and showed low cytotoxicity against HOKs and HGEs.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Microbiology
Livia A. Alves, Geovanny C. Salvatierra, Victor A. Freitas, Jose F. Hoefling, Debora C. Bastos, Thais L. S. Araujo, Renata O. Mattos-Graner
Summary: This study explores the intra-species functional diversity of Streptococcus sanguinis to gain a better understanding of its commensal and pathogenic lifestyles. Through phenotypic screening, conserved and strain-specific functions involved in biofilm formation and persistence in oral and cardiovascular tissues were identified. The study also reveals a correlation between strain invasiveness and C3b binding.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Rong Mu, David Anderson, Justin Merritt, Hui Wu, Jens Kreth
Summary: The study revealed that Streptococcal pyruvate oxidase SpxB is primarily located in the cytoplasm with a small portion associated with the membrane, and undergoes potential post-translational modifications. Mutant SpxB proteins with site-specific mutations exhibited reduced solubility in vivo, leading to observed changes in colony morphology, bacterial growth, and H2O2 production. These findings suggest that post-translational modifications likely play a significant role in regulating SpxB function in Streptococcus sanguinis.
MOLECULAR ORAL MICROBIOLOGY
(2021)
Article
Microbiology
So-Young Ham, Han-Shin Kim, Eunji Cha, Taehyeung Lim, Youngjoo Byun, Hee-Deung Park
Summary: Raffinose, a natural oligosaccharide, strongly inhibits biofilm formation and GTF-related gene expression in Streptococcus mutans, making it a potential antibiofilm agent for oral supplies and dental materials to prevent dental caries.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Irfan A. Rather, Mohammad Younus Wani, Majid Rasool Kamli, Jamal S. M. Sabir, Khalid Rehman Hakeem, Ahmad Firoz, Yong-Ha Park, Yan-Yan Hor
Summary: This study demonstrates the anti-biofilm and anti-virulence properties of Lactiplantibacillus plantarum KAU007 against Streptococcus pyogenes. The results show that Lactiplantibacillus plantarum KAU007 inhibits the growth and survival of S. pyogenes, as well as the formation of biofilm and the production of virulence-associated factors. This research provides a basis for further investigation into the use of Lactiplantibacillus plantarum KAU007 as an antibacterial agent to combat streptococcal infections.
Article
Agriculture, Multidisciplinary
Ji-Eun Lee, Hee Yeon Cho, Jae Hoon Lee, Dong Uk Ahn, Kee-Tae Kim, Hyun-Dong Paik
Summary: This study investigated the inhibitory effects of ovomucoid, a protein found in egg white, on the biofilm formation of Streptococcus mutans, the primary pathogen associated with tooth decay. The results showed that ovomucoid inhibited biofilm formation, reduced bacterial adhesion and aggregation, and lowered the metabolic activity of bacteria in the biofilm. In addition, ovomucoid decreased the synthesis of water-insoluble EPS by S. mutans. Therefore, ovomucoid has the potential to be used as an anti-biofilm agent for the treatment of dental caries.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Microbiology
Chiau-Jing Jung, Chih-Chieh Hsu, Jeng-Wei Chen, Hung-Wei Cheng, Chang-Tsu Yuan, Yu-Min Kuo, Ron-Bin Hsu, Jean-San Chia
Summary: The LiaR-regulated PCP protein in Streptococcus mutans plays a crucial role in bacterial biofilm formation and platelet adhesion. It is well-preserved among pathogenic streptococci and controls biofilm formation in vivo, suggesting its importance in colonization for S. mutans and possibly other pathogenic streptococci.
Article
Biochemistry & Molecular Biology
Pakhshan Abdullah Hassan, Chiman Hameed Saeed, Sirwan Ahmed Rashid, Sawsan Mohammed Sorchee, Suhayla Hamad Shareef
Summary: This study developed a biofilm assay to investigate biofilm formation in Streptococcus sanguinis and found that certain genes may play a crucial role in the formation of biofilms in this bacteria, contributing to increased biofilm formation in gingivitis patients.
CELLULAR AND MOLECULAR BIOLOGY
(2022)
Article
Fisheries
Taylor I. Heckman, Esteban Soto
Summary: The globally distributed bacterium Streptococcus iniae is responsible for outbreaks of disease in economically important freshwater and marine fish species, but our understanding of its transmission and infection dynamics remains incomplete. This study found that S. iniae has the ability to form resilient biofilms, providing an effective mechanism for its persistence in the environment. Further research on controlling this pathogen is necessary.
Article
Food Science & Technology
Yan Ma, Yingying Zhang, Zhongguo Shan, Xin Wang, Xiaodong Xia
Summary: The study investigated the impact of PhoP/PhoQ two component system on biofilm formation in Cronobacter sakazakii BAA-894. Results showed that the system played crucial roles in regulating gene expression, cell viability, and extracellular polymeric substance content, suggesting it as a potential target for controlling biofilms.
Review
Microbiology
Bin Zhu, Lorna C. Macleod, Todd Kitten, Ping Xu
FUTURE MICROBIOLOGY
(2018)
Article
Materials Science, Biomaterials
Donald C. Aduba, Seon-Sook An, Gretchen S. Selders, W. Andrew Yeudall, Gary L. Bowlin, Todd Kitten, Hu Yang
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2019)
Article
Materials Science, Biomaterials
Donald C. Aduba, Seon-Sook An, Gretchen S. Selders, W. Andrew Yeudall, Gary L. Bowlin, Todd Kitten, Hu Yang
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2019)
Article
Biochemistry & Molecular Biology
Ahmad J. Obaidullah, Mostafa H. Ahmed, Todd Kitten, Glen E. Kellogg
CHEMISTRY & BIODIVERSITY
(2018)
Article
Microbiology
Cristina Colomer-Winter, Ana L. Flores-Mireles, Shannon P. Baker, Kristi L. Franks, Aaron J. L. Lynch, Scott J. Hultgren, Todd Kitten, Jose A. Lemos
Article
Immunology
Shannon P. Baker, Tara J. Nulton, Todd Kitten
INFECTION AND IMMUNITY
(2019)
Article
Biochemistry & Molecular Biology
Bin Zhu, Shannon P. Green, Xiuchun Ge, Tanya Puccio, Haider Nadhem, Henry Ge, Liang Bao, Todd Kitten, Ping Xu
Summary: The bacterium Streptococcus sanguinis, usually associated with oral health, may cause infective endocarditis when it enters the bloodstream. Identifying genes required for survival in the blood could provide targets for more effective endocarditis-specific antibiotics. By screening a library of mutants, researchers discovered 178 mutants with significant changes in abundance in human serum, highlighting biological functions disrupted by these mutations.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Meztlli O. Gaytan, Anirudh K. Singh, Shireen A. Woodiga, Surina A. Patel, Seon-Sook An, Arturo Vera-Ponce de Leon, Sean McGrath, Anthony R. Miller, Jocelyn M. Bush, Mark van der Linden, Vincent Magrini, Richard K. Wilson, Todd Kitten, Samantha J. King
Summary: Bacterial binding to platelets is a key step in the development of infective endocarditis. The novel bacterial protein AsaA was identified to help bacteria bind to platelets. Sialic acid on platelets serves as a receptor for a wider range of IE-causing bacteria than previously appreciated.
Article
Endocrinology & Metabolism
Tanya Puccio, Biswapriya B. Misra, Todd Kitten
Summary: Analysis of metabolites in response to manganese depletion revealed quantitative changes in 534 and 422 metabolites in cells and media, respectively. The cellular metabolites identified as significantly different indicated enrichment of purine and pyrimidine metabolism, while multivariate analysis showed that the top 15 cellular metabolites primarily belonged to lipids and redox metabolites.
Article
Biochemistry & Molecular Biology
Tanya Puccio, Karina S. Kunka, Seon-Sook An, Todd Kitten
Summary: In Streptococcus sanguinis, the ABC-family manganese transporter SsaACB is crucial for virulence, while the ZIP-family protein TmpA acts as a secondary manganese transporter. The two proteins function together to maintain bacterial growth and pathogenicity.
MOLECULAR MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tanya Puccio, Seon-Sook An, Alexander C. Schultz, Claudia A. Lizarraga, Ashley S. Bryant, David J. Culp, Robert A. Burne, Todd Kitten
Summary: The study showed that Streptococcus sanguinis' manganese uptake under acidic conditions is regulated by SsaACB, with potential differences in the function of manganese transporters between different strains. Growth and cellular manganese levels may vary under different conditions. The heterogeneity of the oral environment could explain the presence of a variety of manganese transporters in S. sanguinis.
MOLECULAR MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nitya Reddy, Janina Golob Deeb, Todd Kitten, Caroline K. Carrico, Kinga Grzech-Lesniak
Summary: This in vitro study evaluated the effect of different antimicrobial agents combined with Carbon Dioxide (CO2) and Erbium-doped Yttrium Aluminum Garnet (Er:YAG) lasers on the viability of oral bacteria associated with root caries. The results showed that the combination of Er:YAG laser with 0.5% sodium hypochlorite produced the greatest reduction in bacterial survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Janina Golob Deeb, Nitya Reddy, Todd Kitten, Caroline Kelly Carrico, Kinga Grzech-Lesniak
Summary: The study assessed the effect of Nd:YAG laser combined with various antimicrobial agents on the viability of bacteria implicated in root caries. The results showed that Nd:YAG laser with 0.5% NaOCl resulted in the most substantial reduction in bacterial survival.
DENTAL AND MEDICAL PROBLEMS
(2023)
Article
Biotechnology & Applied Microbiology
Riaz Ali, Justin Bartholomew, Randolph Coffey, Caroline Carrico, Todd Kitten, Parthasarathy Madurantakam
Summary: This study investigated the potential of peracetic acid (PA) as an alternative method for reliable disinfection of freshly extracted human teeth. The results indicate that PA can effectively disinfect extracted teeth, providing a reliable alternative to formalin and autoclaving.
BIOENGINEERING-BASEL
(2021)
Article
Microbiology
Tanya Puccio, Karina S. Kunka, Bin Zhu, Ping Xu, Todd Kitten
FRONTIERS IN MICROBIOLOGY
(2020)
