Article
Fisheries
Xiao-Lei Su, Shan-Shan Zhao, Wen-Jing Xu, Liang Shuang, Guo-Dong Zheng, Shu-Ming Zou
Summary: The atmospheric and room temperature plasma (ARTP) mutagenesis technique was used to produce whole-genomic mutants in blunt snout bream. After cultivation, the mutants showed improved performance in terms of growth and hypoxia tolerance. Genome resequencing analysis revealed a high mutation rate and identified genes related to hypoxia and growth with non-synonymous mutations. This novel approach has important implications for genetic breeding.
Article
Biochemistry & Molecular Biology
Han Liu, Chunhai Chen, Maolin Lv, Ning Liu, Yafei Hu, Hailin Zhang, Erik D. Enbody, Zexia Gao, Leif Andersson, Weimin Wang
Summary: This study reports a major OR expansion in a freshwater herbivorous fish, Megalobrama amblycephala, and evaluates the possible contribution of OR expansion to habitat and feeding specialization by comparing the OR repertoire in 28 phylogenetically and ecologically diverse teleosts. The research found a strong association between duplications of beta and epsilon ORs and freshwater habitat, with the expanded beta and epsilon ORs highly expressed in the olfactory epithelium of Megalobrama amblycephala.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biotechnology & Applied Microbiology
Zhiqiang Wang, Yongyi Jia, Xin Huang, Dongmei Zhu, Han Liu, Weimin Wang
Summary: The study utilized Megalobrama amblycephala as a fish model to investigate the developmental regulation mechanism of scales, revealing core features of scale patterning and key regulatory genes involved in scale morphogenesis.
Article
Biotechnology & Applied Microbiology
Shan-Shan Zhao, Xiao-Lei Su, Rong-Jia Pan, Li-Qun Lu, Guo-Dong Zheng, Shu-Ming Zou
Summary: This study used bortezomib to explore the adaptation mechanism of Pujiang No.2 to hypoxia stress and accurately identified the genes related to hypoxia tolerance advantage through transcriptome analysis.
Article
Immunology
Aotian Ouyang, Huabing Wang, Jianguo Su, Xiaoling Liu
Summary: COS6 enters blunt snout bream macrophages through MaMR CTLD4-8, suppressing the expression of inflammatory genes while other receptors are involved in immune regulation. MaMR CTLD4-8 cooperates with TLR4 to regulate the pro-inflammatory response caused by COS6 in macrophages of blunt snout bream.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Xiaohui Xu, Jixiu Wang, Jiaqi Wu, Huanling Wang, Hong Liu
Summary: This study identified and characterized eight STAT genes in blunt snout bream, an economically important fish in China. The results showed the evolutionary origin, tissue expression, and role in pathogen resistance of these genes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jing Chen, Xin Huang, Ruijing Geng, Dongmei Zhu, Weimin Wang, Han Liu
Summary: Ribonuclease 1 (RNase1) is a vertebrate-specific enzyme that shows antimicrobial activity in blunt snout bream (Megalobrama amblycephala) by increasing the permeability of bacterial membranes and reducing tissue damage caused by infections. The study found that RNase1 can up-regulate immune-related gene expression and exert antimicrobial functions both in vitro and in vivo.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Jing Chen, Xiuli Chen, Xin Huang, Guanghua Huang, Zexia Gao, Weimin Wang, Han Liu
Summary: Intermuscular bone (IB) is a hard-boned spicule found in lower teleost, negatively impacting the palatability and economic value of blunt snout bream (Megalobrama amblycephala). Through molecular analysis, key signaling pathways related to IB formation have been identified, indicating a possible involvement of intramembranous ossification in the development process.
Article
Biochemistry & Molecular Biology
Lifang Liu, Yulong Chen, Jinghan Diao, Lifei Luo, Zexia Gao
Summary: This study investigated the regulatory role of circRNAs in fish muscle growth by analyzing the whole transcriptome of skeletal muscles from two populations of Megalobrama amblycephala with different growth rates. The results identified differentially expressed circRNAs related to processes like aminoglycan biosynthesis, cytokinesis, and muscle development pathways, suggesting their involvement in muscle growth regulation. The study also pointed out specific circRNAs that may act as competing endogenous RNAs to control gene expression levels and further understanding circRNAs in M. amblycephala.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Yang Liu, Zuzhen Wang, Wenjun Wang, Bing Liu, Chunfang Li, Yuandong Sun, Jiri Cao, Kuanyu Xia, Moci Yang, Jinpeng Yan
Summary: In this study, a novel C-type lectin (MaCTL) was cloned and characterized, which plays significant roles in innate immune response through binding to the pathogen-associated molecular patterns (PAMPs) on microorganisms. The results showed that MaCTL is widely expressed in various tissues and upregulated after LPS stimulation and Aeromonas hydrophila challenge, suggesting its involvement in innate immune response. Furthermore, MaCTL can bind to various bacteria and carbohydrates, and exhibit antimicrobial effects.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shan-Shan Zhao, Xiao-Lei Su, Hui-Qi Yang, Guo-Dong Zheng, Shu-Ming Zou
Summary: This study identified six hif alpha genes in blunt snout bream, with different expressions under hypoxia conditions. Two SNP sites in hif2 alpha b gene were associated with hypoxia tolerance in a selected strain of blunt snout bream. Diplotype II of hif2 alpha b gene showed better hypoxia tolerance, increased erythrocyte count, enhanced enzyme expression, and higher expression of epo gene.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Fisheries
Hualiang Liang, Xianping Ge, Dong Xia, Mingchun Ren, Haifeng Mi, Liangkun Pan
Summary: The study demonstrated that dietary chromium supplementation had no significant effect on the immune and antioxidant capacities of the fish under normal physiological conditions, but played an important role in relieving heat stress.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Rongjia Pan, Zaozao Guo, Wenya Xu, Shanshan Li, Guodong Zheng, Shuming Zou
Summary: Ammonia-nitrogen is a common stress factor for aquatic organisms, causing ammonia poisoning in fish. This study investigated the effects of high concentrations of ammonia-nitrogen on juvenile blunt snout bream. It was found that the antioxidant systems in various tissues were enhanced initially, but eventually declined, leading to irreversible damage to organs. Additionally, cell apoptosis was induced in different tissues, indicating the activation of the apoptotic system.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Xin-xin Yu, Yan-rui Zhang, Shan-shan Li, Guo-dong Zheng, Shu-ming Zou
Summary: Hypoxia stress changes the gill filament structure of blunt snout bream, leading to an expansion of the lamellar respiratory surface area and a decrease in interlamellar cell mass volume. Hypoxia also increases ROS activity and decreases mitochondrial membrane potential in gill tissue, while decreasing the expression of anti-apoptotic gene Bcl-2 and increasing the expression of pro-apoptotic gene Bax. Furthermore, hypoxia leads to a decreased expression of Phd1 and an increased expression of Hif-1a in gills, indicating enhanced adaptation through the HIF pathway.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Fisheries
Huabing Wang, Feifei Zheng, Aotian Ouyang, Gailing Yuan, Jianguo Su, Xiaoling Liu
Summary: This study cloned and functionally characterized MaCSF-1 and MaCSF-1R from blunt snout bream, and found that they play crucial roles in macrophage proliferation, phagocytosis, and IL-10 production.
FISH & SHELLFISH IMMUNOLOGY
(2022)