Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins

Reproducibility: Standardize antibodies used in research

(2015) Andrew Bradbury et al.   NATURE

Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation

(2015) Edyta Marcon et al.   NATURE METHODS

Tissue-based map of the human proteome

(2015) M. Uhlen et al.   SCIENCE

CDR-H3 Diversity Is Not Required for Antigen Recognition by Synthetic Antibodies

(2012) Helena Persson et al.   JOURNAL OF MOLECULAR BIOLOGY

Generating conformation-specific synthetic antibodies to trap proteins in selected functional states

(2012) Marcin Paduch et al.   METHODS

T Cell Receptor-Like Recognition of Tumor In Vivo by Synthetic Antibody Fragment

(2012) Keith R. Miller et al.   PLoS One

Too many roads not taken

(2011) Aled M. Edwards et al.   NATURE

A roadmap to generate renewable protein binders to the human proteome

(2011) Karen Colwill et al.   NATURE METHODS

Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation

(2011) Tracy Keates et al.   New Biotechnology

Antibody validation

(2010) Jennifer Bordeaux et al.   BIOTECHNIQUES

A Lentiviral Functional Proteomics Approach Identifies Chromatin Remodeling Complexes Important for the Induction of Pluripotency

(2010) Anthony B. Mak et al.   MOLECULAR & CELLULAR PROTEOMICS

A unique chromatin signature uncovers early developmental enhancers in humans

(2010) Alvaro Rada-Iglesias et al.   NATURE

Towards a knowledge-based Human Protein Atlas

(2010) Mathias Uhlen et al.   NATURE BIOTECHNOLOGY

A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation

(2010) Dorothy Beckett et al.   PROTEIN SCIENCE

A Genecentric Human Protein Atlas for Expression Profiles Based on Antibodies

(2008) Lisa Berglund et al.   MOLECULAR & CELLULAR PROTEOMICS

Protein production and purification

(2008) Susanne Gräslund et al.   NATURE METHODS

The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins

(2007) Susanne Gräslund et al.   PROTEIN EXPRESSION AND PURIFICATION