Review
Infectious Diseases
Sara F. Haddad, Daniel C. DeSimone, Supavit Chesdachai, Danielle J. Gerberi, Larry M. Baddour
Summary: Blood culture-negative endocarditis (BCNE) is a common condition in infective endocarditis (IE) cases, often associated with antibiotic exposure. In recent years, molecular techniques such as metagenomic next-generation sequencing (mNGS) have been widely used for pathogen identification in BCNE cases. This systematic review found that mNGS is effective in identifying various pathogenic bacteria in IE cases. However, further research is needed to evaluate the utility of mNGS in BCNE and its impact on patient outcomes.
Article
Immunology
Yuru Shi, Jing Yang, Yingjie Qi, Junlan Xu, Yingqi Shi, Tiantian Shi, Chao Liu, Xiaoling Ma
Summary: Bartonella species, transmitted by blood-sucking arthropods, can cause various diseases. This study reports a case of Bartonella vinsonii infection in a patient with AIDS, describing the clinical manifestations and management.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Immunology
Zhouqing Gan, Jia Liu, Yijie Wang, Lu Yang, Zheng Lou, Han Xia, Min Li, Zhuolin Chen, Ying Jiang, Fuhua Peng
Summary: This study demonstrates that cerebrospinal fluid (CSF) metagenomic next-generation sequencing (mNGS) can be considered as a supplementary test for the diagnosis of cryptococcal meningitis (CM) and for directly distinguishing Cryptococcus gattii from Cryptococcus neoformans in clinical specimens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Yi-Yi Qian, Hong-Yu Wang, Yang Zhou, Hao-Cheng Zhang, Yi-Min Zhu, Xian Zhou, Yue Ying, Peng Cui, Hong-Long Wu, Wen-Hong Zhang, Jia-Lin Jin, Jing-Wen Ai
Summary: Metagenomic next generation sequencing (mNGS) has shown higher sensitivity and better diagnostic performance in detecting pathogens in pulmonary infections, especially for rare and emerging pathogens. It provides reliable phylogenetic and epidemiological information, with a shorter turnaround time, and can complement traditional methods to improve the identification of respiratory infection agents.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Zexin Tao, Xiaojuan Lin, Yao Liu, Feng Ji, Suting Wang, Ping Xiong, Li Zhang, Qing Xu, Aiqiang Xu, Ning Cui
Summary: This study collected sewage samples from Jinan, China from 2018 to 2019, and analyzed classic and novel HAstVs using real-time quantitative PCR and next generation sequencing. The results showed that sewage contains a high diversity of astroviruses, and there are multiple co-circulating lineages within each local HAstV genotype.
Article
Immunology
Min Kong, Wei Li, Qingsheng Kong, Haixin Dong, Aizhong Han, Liqing Jiang
Summary: This study describes a case of a child infected with tuberculosis after being wounded. The use of metagenomic next-generation sequencing (mNGS) helped in identifying the specific pathogen and facilitating early treatment, leading to improved prognosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Bin Fang, Juan Lai, Yongfeng Liu, Tian-tian Yu, Xiao Yu, Xiang Li, Lijun Dong, Xin Zhang, Wei Yang, Qin Yan, Lei Sun, Lin-lin Liu
Summary: This study characterized the genomic epidemiology of human adenoviruses (HAdVs) in Hubei, China using metagenomic next-generation sequencing (mNGS). The most common genotypes were HAdV-B3 and HAdV-C2, and three recombinant strains were identified. This study is important for understanding the genomic variation and recombination of HAdVs, and highlights the need for epidemiological surveillance in China.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Yunqi Zhu, Wenxuan Zhao, Xihong Yang, Yuanyuan Zhang, Xiaoling Lin, Xing Weng, Yali Wang, Cong Cheng, Yun Chi, Hongxia Wei, Zhihang Peng, Zhiliang Hu
Summary: This study found that mNGS has a high sensitivity for detecting common opportunistic CNS pathogens in HIV-infected patients, although its performance in CNS tuberculosis is unsatisfactory. EBV and CMV are commonly detected by CSF mNGS, but the threshold for clinically significant detection still needs to be defined.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jung-Yien Chien, Chong-Jen Yu, Po-Ren Hsueh
Summary: The performance of metagenomic next-generation sequencing (mNGS) was evaluated and compared with that of conventional culture testing in patients with sepsis. mNGS had higher detection rates than blood culture and BALF culture, especially for bacteria, viruses, and multipathogen infections. RNA-based mNGS increased the detection rate of several bacteria, fungi, and viruses. mNGS offers a sensitive diagnostic method for patients with sepsis, but clinical correlation is advised to interpret the data due to the lack of unified diagnostic criteria.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Ying Liu, Huifen Wang, Yaoguang Li, Zujiang Yu
Summary: The study aimed to evaluate the clinical diagnostic value of metagenomic next-generation sequencing (mNGS) for tuberculosis (TB). Retrospective analysis of 52 patients with suspected TB infection was conducted, and multiple detection methods were performed. The results showed that mNGS had a higher detection rate of MTB compared to acid-fast staining and PCR, but no statistical difference with T-SPOT.TB assay. As an adjunctive diagnostic technology, mNGS can be combined with traditional methods to guide the diagnosis and treatment of TB.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Dongsheng Han, Zhenli Diao, Huiying Lai, Yanxi Han, Jiehong Xie, Rui Zhang, Jinming Li
Summary: The performance of 90 mNGS laboratories under routine testing conditions is evaluated by analyzing identical samples. The results showed high interlaboratory variability in identifying microbes and quantifying reads per million reads (RPM) values, particularly for low-concentration microbes. 42.2% of the laboratories reported false positive problems, and only a portion of the laboratories demonstrated sufficient ability to provide accurate diagnoses and distinguish genetically similar organisms. The findings highlight the need for improving the accuracy and comparability of mNGS results across different laboratories, especially for low-microbial-biomass samples.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Immunology
Mengyi Zhao, Ruiming Yue, Xiaoxiao Wu, Zhan Gao, Miao He, Lingai Pan
Summary: mNGS testing of bronchoalveolar lavage fluid (BALF) or blood achieved effective detection rate in PJP patients. The study also confirmed the correlation between the abundance of P. jirovecii genome reads and the interval between onset and sample collection, and the inflammation status might affect the abundance of pathogens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Jingying Xu, Qian Huang, Jianhua Yu, Shourong Liu, Zongxing Yang, Fei Wang, Yue Shi, Er Li, Zhaoyi Li, Yunlei Xiao
Summary: This study explored the applicability of mNGS as a diagnostic tool for suspected opportunistic infections in HIV patients. The results showed that mNGS had a higher detection rate for fungi and nontuberculous mycobacteria compared to CMTs. The sensitivity of mNGS was high for detecting fungi, bacteria, nontuberculous mycobacteria, and viruses. The combination of mNGS and CMTs may be the best diagnosis strategy.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Yirui Xie, Bohao Dai, Xiaotang Zhou, Huiting Liu, Wei Wu, Fei Yu, Biao Zhu
Summary: mNGS has a higher diagnostic accuracy for HIV-infected patients with suspected mixed pneumonia, especially in detecting fungal pathogens. It can identify more co-pathogens in mixed infections compared to conventional microbiological tests (CMTs). Early performance of bronchoscopy and mNGS are recommended in HIV-infected patients with low CD4 T cell counts and long duration of symptoms.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Public, Environmental & Occupational Health
Xianghong Liu, Ye Zhang, Jun Zhang, Zheng Lou, Han Xia, Zhijuan Lu
Summary: The study utilized metagenomic next-generation sequencing (mNGS) and other diagnostic methods to identify pathogens in patients suspected of scrub typhus. The results showed that mNGS outperformed traditional diagnostic methods, providing a better early diagnosis of scrub typhus and improving diagnostic accuracy.
FRONTIERS IN PUBLIC HEALTH
(2021)