Article
Immunology
Do Thi Thuy Nga, Phung Thi Bich Thuy, Akira Ainai, Ikuyo Takayama, Do Thu Huong, Aya Saitoh, Satoshi Nakagawa, Tran Minh Dien, Noriko Nakajima, Akihiko Saitoh
Summary: This study found that real-time PCR Ct values could be a useful predictor of severe disease in neonates and infants with pertussis.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2022)
Article
Microbiology
Yifang Cui, Fangfang Guo, Xuwang Cai, Xiaoya Cao, Jie Guo, Hongjun Wang, Bing Yang, Hongzhuan Zhou, Xia Su, Patrick J. Blackall, Fuzhou Xu
Summary: Glaesserella parasuis is the causative agent of Glasser's disease in swine. Molecular serotyping based on variation within the capsule loci is more accurate and efficient than traditional serological serotyping. The developed qPCR method showed high accuracy in distinguishing between G. parasuis serovars and was effective in clinical isolates as well.
VETERINARY MICROBIOLOGY
(2021)
Article
Virology
Kubra Aykac, Burcu Ceylan Cura Yayla, Yasemin Ozsurekci, Kubra Evren, Pembe Derin Oygar, Sibel Lacinel Gurlevik, Tugce Coskun, Onur Tasci, Filiz Demirel Kaya, Ilknur Fidanci, Medine Aysin Tasar, Alpaslan Alp, Ali Bulent Cengiz, Sevilay Karahan, Mehmet Ceyhan
Summary: The study found that the viral load of COVID-19 in pediatric patients remains similar regardless of age and underlying diseases, indicating that children may play a role in the transmission chain regardless of disease severity.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Gabriel E. Wagner, Johanna Dabernig-Heinz, Michaela Lipp, Adriana Cabal, Jonathan Simantzik, Matthias Kohl, Martina Scheiber, Sabine Lichtenegger, Ralf Ehricht, Eva Leitner, Werner Ruppitsch, Ivo Steinmetz
Summary: This study finds that nanopore technology can be used for high-resolution bacterial typing, with comparable accuracy to traditional technologies in outbreak and pathogen variant analysis. Furthermore, by combining real-time data processing, this technology allows for fast and accurate typing of bacterial pathogens while sequencing is still in progress.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Maya Makov-Assif, Sydney Krispin, Yatir Ben-Shlomo, Tal Holander, Noa Dagan, Ran Balicer, Noam Barda
Summary: The study revealed a significant association between low Ct values and severe COVID-19 outcomes in community diagnosed cases, showing a dose-response relationship where the risk increases gradually with lower Ct values. This suggests that Ct values could be useful in identifying high-risk patients in the community.
INFECTIOUS DISEASES
(2022)
Article
Medical Laboratory Technology
Mati Ur Rehman, Syed Sajjad Naqvi, Rooh Ullah, Narmeen Arshad, Muhammad Ammad, Qurat Ul Ain, Anam Razzak, Muhammad Yousaf, Arif Hussain, Tarique N. Hasan
Summary: This study investigated the association between SARS-CoV-2 cycle threshold and the age and gender of COVID-19 patients. It found that males were more susceptible to COVID-19 and had slightly higher viral loads compared to females. The study also found no significant difference in viral loads between different age groups. These findings have implications for preventive strategies.
CLINICA CHIMICA ACTA
(2022)
Article
Meteorology & Atmospheric Sciences
Henri Pinheiro, Manoel Gan, Kevin Hodges
Summary: This study examines the three-dimensional structure and evolution of the 200 most intense Cut-off Lows (COLs) in the Southern Hemisphere (SH). The results show a well-defined tropospheric moist cold core co-located with warm dry air in the lower stratosphere, surrounded by regions of strong temperature gradients (frontal zones) which move downstream throughout the life cycle. Precipitation is related to relative vorticity and precipitable water, with maximum precipitation observed during decay.
QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY
(2021)
Article
Immunology
John Strutner, Nanda Ramchandar, Shruti Dubey, Mary Gamboa, Michelle K. Vanderpool, Teresa Mueller, Wei Wang, Christopher Cannavino, Leidy Tovar Padua, Denise Malicki, Alice Pong
Summary: This study compared PCR Ct values of symptomatic and asymptomatic children infected with SARS-CoV-2, showing significantly lower Ct values in symptomatic patients and the lowest Ct values in children under 5 years of age. This suggests that symptomatic children and younger children with SARS-CoV-2 may have a higher viral load in the nasopharynx compared to asymptomatic children, highlighting the need for further studies on transmission potential.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Kazunari Kamachi, Kentaro Koide, Nao Otsuka, Masataka Goto, Tsuyoshi Kenri
Summary: Bordetella pertussis is a highly infectious bacterium that easily causes pertussis outbreaks in humans, especially in school-aged children. In detection and investigation of outbreaks, excluding non-outbreak isolates is important for understanding the bacterial transmission routes.
MICROBIOLOGY SPECTRUM
(2023)
Review
Medicine, General & Internal
Ali A. Rabaan, Raghavendra Tirupathi, Anupam A. Sule, Jehad Aldali, Abbas Al Mutair, Saad Alhumaid, Muzaheed, Nitin Gupta, Thoyaja Koritala, Ramesh Adhikari, Muhammad Bilal, Manish Dhawan, Ruchi Tiwari, Saikat Mitra, Talha Bin Emran, Kuldeep Dhama
Summary: Real-time RT-PCR is considered the gold standard confirmatory test for COVID-19, but there is disagreement among scientists about its reliability. Ct values are being used to diagnose or predict SARS-CoV-2 infection and are correlated with viral load and various hematological and biochemical markers. Numerous factors can influence Ct values and their interpretation plays a crucial role in determining viral load and disease severity.
Review
Public, Environmental & Occupational Health
Yu-Pei Yang, Zhu Liduzi Jiesisibieke, Tao-Hsin Tung
Summary: This study aimed to assess the association between rapid antigen detection tests (RADTs) and real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A meta-analysis of fifty-one studies showed a satisfactory overall false positive rate of RADTs compared to RT-PCR. Subgroup analysis based on different RT-PCR Ct value cut-offs (<40 or ≥40) also showed consistent results with no increase in the false positive rates of RADTs.
INTERNATIONAL JOURNAL OF PUBLIC HEALTH
(2023)
Article
Pathology
Andrew Fox-Lewis, Shivani Fox-Lewis, Jenna Beaumont, Dragana Drinkovic, Jay Harrower, Kevin Howe, Catherine Jackson, Fahimeh Rahnama, Blair Shilton, Helen Qiao, Kevin K. Smith, Susan C. Morpeth, Susan Taylor, Matthew Blakiston, Sally Roberts, Gary McAuliffe
Summary: This study aimed to describe the dynamics of SARS-CoV-2 upper respiratory tract viral load in the New Zealand Northern Region. The findings suggest that shortly after symptom onset is the optimal time to test for SARS-CoV-2, with Ct values remaining useful until around 15 days post-symptom onset. The study also indicates that using Ct values alone to determine time post-infection is not sufficient to rule out infectivity.
Article
Infectious Diseases
Shimon Edelstein, Miriam Sudri, Shibli Tanous, Hila Ben Amram, Adi Sharabi-Nov, Inna Rozenfeld, Hedva Halal, Salman Zarka
Summary: This study examines the impact of sample collection methods on the results of PCR tests. The findings demonstrate that rigorous sample collection methods lead to lower cycle threshold values. Therefore, it is important to prioritize the method of sample collection in places where accurate detection of disease onset is crucial, in order to minimize the risk of false negatives.
Article
Virology
Jessica Penney, Amanda Jung, Benjamin Koethe, Shira Doron
Summary: In this study, it was found that Ct value is not associated with demographic characteristics, but increases with time since symptom onset in SARS-CoV-2 infection. Additionally, increasing Ct value is associated with increased odds of severe disease.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Microbiology
Philip J. Schmidt, Nicole Acosta, Alex H. S. Chik, Patrick M. D'Aoust, Robert Delatolla, Hadi A. Dhiyebi, Melissa B. Glier, Casey R. J. Hubert, Jennifer Kopetzky, Chand S. Mangat, Xiao-Li Pang, Shelley W. Peterson, Natalie Prystajecky, Yuanyuan Qiu, Mark R. Servos, Monica B. Emelko
Summary: Real-time polymerase chain reaction (qPCR) is commonly used in environmental microbiology, including the detection and quantification of SARS-CoV-2 during the COVID-19 pandemic. However, the traditional log-linear standard curve model used in qPCR has limitations in explaining data at low concentrations. By integrating probabilistic modeling concepts, a new model has been developed that can better represent standard curve data at low concentrations while maintaining compatibility with the log-linear regression model at high concentrations. This improved modeling approach allows for exploration of random error mechanisms and quantification of uncertainty in qPCR-based concentration estimates.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Taryn B. T. Athey, Sarah Teatero, Lee E. Sieswerda, Jonathan B. Gubbay, Alex Marchand-Austin, Aimin Li, Jessica Wasserscheid, Ken Dewar, Allison McGeer, David Williams, Nahuel Fittipaldi
JOURNAL OF CLINICAL MICROBIOLOGY
(2016)
Article
Respiratory System
Sarah K. Brode, Frances B. Jamieson, Ryan Ng, Michael A. Campitelli, Jeffrey C. Kwong, J. Michael Paterson, Ping Li, Alex Marchand-Austin, Claire Bombardier, Theodore K. Marras
Article
Medicine, General & Internal
Kevin L. Schwartz, Jeffrey C. Kwong, Shelley L. Deeks, Michael A. Campitelli, Frances B. Jamieson, Alex Marchand-Austin, Therese A. Stukel, Laura Rosella, Nick Daneman, Shelly Bolotin, Steven J. Drews, Heather Rilkoff, Natasha S. Crowcroft
CANADIAN MEDICAL ASSOCIATION JOURNAL
(2016)
Letter
Respiratory System
Theodore K. Marras, Michael A. Campitelli, Jeffrey C. Kwong, Hong Lu, Sarah K. Brode, Alex Marchand-Austin, Andrea S. Gershon, Frances B. Jamieson
EUROPEAN RESPIRATORY JOURNAL
(2016)
Article
Computer Science, Information Systems
Jennifer L. Guthrie, David C. Alexander, Alex Marchand-Austin, Karen Lam, Michael Whelan, Brenda Lee, Colin Furness, Elizabeth Rea, Rebecca Stuart, Julia Lechner, Monali Varia, Jennifer McLean, Frances B. Jamieson
JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION
(2017)
Article
Immunology
Natalie Bocking, Cai-lei Matsumoto, Kassandra Loewen, Sarah Teatero, Alex Marchand-Austin, Janet Gordon, Nahuel Fittipaldi, Allison McGeer
OPEN FORUM INFECTIOUS DISEASES
(2017)
Article
Microbiology
Alex Marchand-Austin, Raymond S. W. Tsang, Jennifer L. Guthrie, Jennifer H. Ma, Gillian H. Lim, Natasha S. Crowcroft, Shelley L. Deeks, David J. Farrell, Frances B. Jamieson
JOURNAL OF CLINICAL MICROBIOLOGY
(2017)
Article
Microbiology
Alefiya Neemuchwala, Deidre Soares, Vithusha Ravirajan, Alex Marchand-Austin, Julianne V. Kus, Samir N. Patel
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Virology
Alefiya Neemuchwala, Venkata R. Duvvuri, Alex Marchand-Austin, Aimin Li, Jonathan B. Gubbay
JOURNAL OF MEDICAL VIROLOGY
(2015)
Article
Multidisciplinary Sciences
James Wilton, Juan Liu, Ashleigh Sullivan, Beth Rachlis, Alex Marchand-Austin, Madison Giles, Lucia Light, Claudia Rank, Ann N. Burchell, Sandra Gardner, Doug Sider, Mark Gilbert, Abigail E. Kroch
Article
Immunology
Natasha S. Crowcroft, Kevin L. Schwartz, Cynthia Chen, Caitlin Johnson, Ye Li, Alex Marchand-Austin, Shelly Bolotin, Frances B. Jamieson, Steven J. Drews, Margaret L. Russell, Lawrence W. Svenson, Kimberley Simmonds, Salaheddin M. Mahmud, Jeffrey C. Kwong
Article
Medicine, General & Internal
Juan Liu, James Wilton, Ashleigh Sullivan, Alex Marchand-Austin, Beth Rachlis, Madison Giles, Lucia Light, Doug Sider, Abigail E. Kroch, Mark Gilbert, Jean Bacon, Lucia Light, Abigail Kroch, Madison Giles, Joanne Lush, Ken English, Michelle Murti, Vanessa Allen, Tony Mazzulli, Mike Mendaglio, Chris Archibald
Article
Immunology
Theodore K. Marras, Michael A. Campitelli, Hong Lu, Hannah Chung, Sarah K. Brode, Alex Marchand-Austin, Kevin L. Winthrop, Andrea S. Gershon, Jeffrey C. Kwong, Frances B. Jamieson
EMERGING INFECTIOUS DISEASES
(2017)
Article
Public, Environmental & Occupational Health
Ye Li, Michael Whelan, Leigh Hobbs, Wen Qi Fan, Cecilia Fung, Kenny Wong, Alex Marchand-Austin, Tina Badiani, Ian Johnson
CANADIAN JOURNAL OF PUBLIC HEALTH-REVUE CANADIENNE DE SANTE PUBLIQUE
(2016)
Article
Immunology
Matthew German, Romy Olsha, Erik Kristjanson, Alex Marchand-Austin, Adriana Peci, Anne-Luise Winter, Jonathan B. Gubbay
EMERGING INFECTIOUS DISEASES
(2015)