Article
Chemistry, Analytical
Yang Xu, Huiqiang Wang
Summary: A portable glucometer-based immunosensor was designed for quantitative detection of tumor markers, showing advantages of low cost, high efficiency, and simple operation, with potential for home use in tracking treatment effects in a timely manner.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Dennis G. Moledina, Wassim Obeid, Rex N. Smith, Ivy Rosales, Meghan E. Sise, Gilbert Moeckel, Michael Kashgarian, Michael Kuperman, Kirk N. Campbell, Sean Lefferts, Kristin Meliambro, Markus Bitzer, Mark A. Perazella, Randy L. Luciano, Jordan S. Pober, Lloyd G. Cantley, Robert B. Colvin, F. Perry Wilson, Chirag R. Parikh
Summary: In this study, urinary CXCL9 was identified as a diagnostic biomarker for acute tubulointerstitial nephritis (AIN) using urine proteomics. This finding was confirmed in multiple validation cohorts and higher expression of CXCL9 was observed in kidney biopsies from AIN patients. This discovery has important implications for the early diagnosis and treatment of AIN.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Chemistry, Analytical
Jing Wang, Kai Mu, Hongjuan Wei, Hong Chen, Yunxiang Wang, Wuxing Zhang, Zhen Rong
Summary: In this study, a paper-based colloidal gold multiplex vertical flow assay was developed for rapid detection of three AKI biomarkers. The assay platform is easy to use, rapid, and low cost, making it a promising diagnostic tool for multiplex detection of disease biomarkers.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Cell Biology
Seung-Hwan Jeong, Ja Hyeon Ku
Summary: This paper reviewed urinary markers for bladder cancer and compared their efficacies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Zhenrui Xue, Minli You, Ping Peng, Haoyang Tong, Wanghong He, Ang Li, Ping Mao, Ting Xu, Feng Xu, Chunyan Yao
Summary: The study aimed to develop a simple and highly specific qPCR-based single-base mutation detection method, which successfully established the Taqman-MGB nanoPCR system by adding different concentrations of gold nanoparticles, improving the specificity of mutation detection with a performance of detecting mutations as low as 0.95%.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Natalia Chebotareva, Anatoliy Vinogradov, Valerie McDonnell, Natalia V. Zakharova, Maria I. Indeykina, Sergey Moiseev, Evgeny N. Nikolaev, Alexey S. Kononikhin
Summary: CKD is a non-specific kidney disease that causes gradual decline in kidney function, posing significant risks for death and other complications. Protein/peptide biomarkers can be used for early non-invasive diagnosis and may provide new approaches for targeted therapy. Mass spectrometry approaches offer valuable insights into understanding CKD progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medical Laboratory Technology
Lingfeng Zeng, Cheuk-Chun Szeto
Summary: Podocytes play a crucial role in maintaining kidney function and are the focus of many kidney diseases. Urinary podocyte-specific markers have been studied for diagnosis and monitoring of kidney diseases, although further research is needed for standardization and automation of laboratory methods. Despite some techniques being expensive or labor-intensive currently, advancements in technology may make them more widely available in the future.
CLINICA CHIMICA ACTA
(2021)
Article
Medicine, General & Internal
Kai-Fan Tsai, Pai-Chin Hsu, Chien-Te Lee, Chia-Te Kung, Yi-Chin Chang, Lung-Ming Fu, Yu-Che Ou, Kuo-Chung Lan, Tzung-Hai Yen, Wen-Chin Lee
Summary: This study investigated the association between cadmium exposure and chronic kidney disease (CKD). Urinary cadmium levels were found to be positively correlated with urinary kidney injury molecule-1 (KIM-1) levels, indicating early renal injury. Age was also independently associated with urinary cadmium levels.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Medicine, General & Internal
Zohaib Saddique, Muhammad Faheem, Amir Habib, Iftikhar UlHasan, Adnan Mujahid, Adeel Afzal
Summary: In the post-pandemic era, point-of-care (POC) diagnosis of diseases using portable electrochemical (bio)sensors is an important research frontier. This article provides a critical review of electrochemical creatinine (bio)sensors, discussing the different receptors and electrochemical devices used, as well as their limitations. The challenges in developing affordable and deliverable creatinine diagnostics and the drawbacks of enzyme-based and enzymeless electrochemical biosensors are elaborated. These devices have potential biomedical applications for early diagnosis of chronic kidney disease and routine monitoring of creatinine in at-risk individuals.
Article
Biochemistry & Molecular Biology
Ramio Cabello, Miguel Fontecha-Barriuso, Diego Martin-Sanchez, Ana M. Lopez-Diaz, Susana Carrasco, Ignacio Mahillo, Carmen Gonzalez-Enguita, Maria D. Sanchez-Nino, Alberto Ortiz, Ana B. Sanz
Summary: CypA is a potential biomarker of kidney injury, released by kidney tubular cells during different forms of cell death, and increased during IRI-induced clinical kidney injury independently from kidney function parameters.
Article
Chemistry, Multidisciplinary
Marcia Dominguez, Kathleen Meyer, Felix Sancenon, Juan F. Blandez, Manuel Serrano, Ramon Martinez-Manez
Summary: Kidney damage can be monitored using different biomarkers in blood, urine or serum, and kidney failure leads to the overexpression of the alanine aminopeptidase (APN) enzyme in urine. In this study, a molecular probe (NB-ALA) based on the Nile Blue fluorophore (NB) was designed to detect APN enzyme in urine through simple fluorometric measurements.
CHEMICAL COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Cristina Sole, Ann T. Ma, Elsa Sola, Marta Carol, Nuria Fabrellas, Adria Juanola, Laura Napoleone, Jordi Gratacos-Gines, Octavi Bassegoda, Marta Cervera, Martina Perez, Ana Belen Rubio, Emma Avitabile, Manuel Morales-Ruiz, Isabel Graupera, Elisa Pose, Patrick S. Kamath, Pere Gines
Summary: The presence of tubular injury in patients with acute kidney injury due to hepatorenal syndrome (AKI-HRS) is controversial. The persistence of AKI-HRS is associated with high short-term mortality, and biomarker levels were significantly lower in AKI-HRS compared to AKI owing to acute tubular necrosis (AKI-ATN).
LIVER INTERNATIONAL
(2021)
Article
Acoustics
Mehmet Ali Aslaner, Emre Yasar, Isa Kilicaslan, Mahi Nur Cerit, Sadik Volkan Emren, Basak Yuksek, Busegul Karakok, Bekir Alperen Baykus, Fikret Bildik, Galip Guz, Ayfer Keles, Ahmet Demircan
Summary: This study demonstrated that multi-organ point-of-care ultrasound is effective in diagnosing different subgroups of acute kidney injury in emergency department patients, with each subgroup having specific diagnostic parameters.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2022)
Article
Biology
Anton I. Korbut, Vyacheslav V. Romanov, Vadim V. Klimontov
Summary: This study assessed the urinary excretion of markers and mediators of tubular injury and renal fibrosis in patients with type 2 diabetes (T2D) and non-albuminuric and albuminuric patterns of chronic kidney disease (CKD). The results demonstrate that elevated urinary excretions of the markers of tubular injury (RBP-4, GST-pi) and renal fibrosis (Col1, Col4), as well as HGF, an antifibrotic regulator, are associated with the albuminuric pattern of CKD in subjects with T2D.
Review
Medicine, General & Internal
Zunzhen Zhou, Dailiang Zhang, Yongjing Wang, Chongzhi Liu, Limei Wang, Yi Yuan, Xiaodan Xu, Yuan Jiang
Summary: Drug-induced nephrotoxicity is a significant concern in clinical medication. Urinary exosomes show potential as biomarkers for predicting drug-induced nephrotoxicity.
FRONTIERS IN MEDICINE
(2023)
Article
Multidisciplinary Sciences
Kyungah Choi, Cheong Shin, Taesu Kim, Hyun Jung Chung, Hyeon-Jeong Suk
SCIENTIFIC REPORTS
(2019)
Article
Biochemical Research Methods
Seunggyu Kim, Seokhun Lee, Ju-Kang Kim, Hyun Jung Chung, Jessie S. Jeon
Article
Biochemical Research Methods
Jeonghun Oh, Jea Sung Ryu, Moosung Lee, Jaehwang Jung, SeungYun Han, Hyun Jung Chung, Yongkeun Park
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Materials Science, Biomaterials
Marcel Janis Beha, Jea Sung Ryu, Yang Soo Kim, Hyun Jung Chung
Summary: The study introduces multi-layer coated gold nanoparticles delivering antisense oligonucleotides targeting resistance genes, demonstrating efficient internalization into various types of Gram-positive bacteria with high selectivity and dose-dependence. This approach shows great potential for targeting various MDR bacterial infections through specific gene silencing, as a combinatorial therapeutic with conventional antibiotics.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Chemistry, Physical
Juhee Lee, Yoo Kyung Kang, Eonju Oh, Juhee Jeong, San Hae Im, Duk Ki Kim, Haeshin Lee, Sang-Gyu Kim, Keehoon Jung, Hyun Jung Chung
Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.
CHEMISTRY OF MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Yoo Kyung Kang, Juhee Lee, San Hae Im, Joo Hoon Lee, Juhee Jeong, Duk Ki Kim, Seung Yun Yang, Keehoon Jung, Sang-Gyu Kim, Hyun Jung Chung
Summary: A Cas9 conjugate complex system was developed to induce efficient HDR editing with minimal carrier material. The Cas9-LP complexes showed lower cytotoxicity compared to conventional lipofectamine formulations and achieved efficient base correction of the RFP gene in HEK293T cells.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Min Jeong Shin, San Hae Im, Wantae Kim, Hyungju Ahn, Tae Joo Shin, Hyun Jung Chung, Dong Ki Yoon
Summary: We demonstrate a facile method to fabricate a recyclable cell-alignment scaffold using nanogrooves based on sublimable liquid crystal (LC) material. The scaffold can efficiently induce directed cell growth and can be used repeatedly for different cell types. Depending on the nanotopography of the LC structures, cells exhibit different morphologies and gene expression patterns. Furthermore, the scaffold can also form cell sheets and maintain the organization.
Article
Optics
Geon Kim, Daewoong Ahn, Minhee Kang, Jinho Park, DongHun Ryu, YoungJu Jo, Jinyeop Song, Jea Sung Ryu, Gunho Choi, Hyun Jung Chung, Kyuseok Kim, Doo Ryeon Chung, In Young Yoo, Hee Jae Huh, Hyun-seok Min, Nam Yong Lee, YongKeun Park
Summary: The healthcare industry urgently needs rapid microbial identification techniques for treating microbial infections. This study proposes a microscopy-based framework that can identify pathogens from single cells or clusters, providing an effective tool for clinical applications.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Biochemical Research Methods
Ha Neul Lee, Juhee Lee, Yoo Kyung Kang, Joo Hoon Lee, Seungju Yang, Hyun Jung Chung
Summary: In this study, we developed a modified isothermal nucleic acid amplification method integrated with lateral flow assay (LFA) for rapid and simple genetic testing. The method involved the design of padlock probes for target DNA ligation, followed by rolling circle amplification (RCA) using capture ligand-modified oligonucleotides as primers. The amplified target DNA was then detected using gold nanoparticles on an LFA membrane strip. We successfully validated the method for detecting the mecA gene, which is associated with methicillin-resistant Staphylococcus aureus (MRSA). We also demonstrated the feasibility of using the method for viral gene targets, such as the RNA-dependent RNA polymerase (RdRp) and receptor binding domain (RBD) genes of SARS-CoV-2.
Article
Chemistry, Analytical
Hyeong Jun Jo, Jea Sung Ryu, Akhmad Irhas Robby, Yang Soo Kim, Hyun Jung Chung, Sung Young Park
Summary: A rapid wireless electrochemical biosensor has been developed to detect and discriminate between gram-negative and gram-positive bacteria, specifically for the selective detection of pneumonia pathogens in human sputum. The sensor shows high sensitivity, selectivity, and detection capabilities for antibiotic-resistant bacteria, as well as in complex media such as human serum. Integration with a wireless sensing system enables real-time monitoring via a smartphone. This bacterial sensor holds potential for rapid and accurate diagnosis of bacterial infections in clinical settings.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Min Jeong Shin, San Hae Im, Baekman Kim, Jieun Choi, Stephani Edwina Lucia, Wantae Kim, Jesse G. Park, Pilhan Kim, Hyun Jung Chung, Dong Ki Yoon
Summary: Using improper wound care materials may hinder wound healing and cause scar formation and infection. This study introduces a simple method to create a cell alignment scaffold that enhances cell growth and migration, facilitating the reproduction of cellular arrangements and tissue restoration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Joo Hoon Lee, Jea Sung Ryu, Yoo Kyung Kang, Haeshin Lee, Hyun Jung Chung
Summary: Biological catecholamines have different effects in the skin and brain, where they can be oxidatively polymerized to form melanin in the skin and are suppressed in the brain. Bacterial growth significantly influences dopamine polymerization, and the relationship between dopamine formation, oxygen levels, and fluorescence can be used to detect antibiotic-resistant bacteria.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Biophysics
Yoo Kyung Kang, San Hae Im, Jea Sung Ryu, Jaehyun Lee, Hyun Jung Chung
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Materials Science, Biomaterials
Jea Sung Ryu, San Hae Im, Yoo Kyung Kang, Yang Soo Kim, Hyun Jung Chung
BIOMATERIALS SCIENCE
(2020)
Article
Biochemical Research Methods
Seunggyu Kim, Fahim Masum, Ju-Kang Kim, Hyun Jung Chung, Jessie S. Jeon