Article
Fisheries
Shuangshuang Gao, Mingshu Yang, Kaijian Xu, Xiaodong Jiang, Xugan Wu, Yongjie Wang
Summary: A study on the intestinal bacterial communities of juvenile Chinese mitten crab investigated the effects of dietary supplementation with different amounts of micro-algal astaxanthin on crab hemolymph antioxidant capacity and immunity. The results showed that astaxanthin supplementation enriched the number of Bacteroidetes members, decreased the relative abundance of potential pathogenic bacteria, and improved the metabolic capacity of the intestinal bacteria. These improvements were correlated with the crab's hemolymph antioxidant capacity.
Article
Multidisciplinary Sciences
Yingkai Xu, Ziwei Huang, Baoli Zhang, Changyue Yu, Lisong Li, Xiaodong Li, Yingdong Li
Summary: This study investigated the effects of different feeding times on the composition of intestinal bacterial communities in Chinese mitten crabs. The results showed that different feeding times changed the intestinal flora composition of the crabs and identified specific feeding times associated with a more significant weight gain effect.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Zhiqiang Li, Zhigang Zhao, Liang Luo, Shihui Wang, Rui Zhang, Kun Guo, Yuhong Yang
Summary: This study investigated the effects of heat stress on the immune function, metabolism, and intestinal microbiota of Chinese mitten crab. The results showed that heat stress significantly affected the immune function and composition of the intestinal microbiota in this species.
Article
Fisheries
Kun Guo, Zhigang Zhao, Liang Luo, Shihui Wang, Rui Zhang, Wei Xu, Guo Qiao
Summary: This study found that aerial exposure stress affects oxidative damage and antioxidant capacity in the hepatopancreas of Eriocheir sinensis; immune-related enzyme activity and intestinal microbiota composition are also altered.
Article
Environmental Sciences
Yue Sun, Chunying Yuan, Qingman Cui
Summary: The study found that thiamethoxam has acute toxic effects on Eriocheir sinensis, reducing their survival rate and impairing their immune and metabolic systems. Higher concentrations of thiamethoxam resulted in a decrease in immune cells and affected enzyme activity and antioxidant capacity in crabs.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Xinping Guo, Ziang Qian, Qiqi Pan, Yuqing Hu, Wangxin Mei, Xiumei Xing, Shaowu Yin, Jie Ji, Kai Zhang
Summary: Excessive use of antibiotics in aquaculture leads to antibiotic residues in aquatic animal products, which poses a threat to human health. This study investigated the effects of florfenicol (FF) on Chinese mitten crabs, focusing on gut health, microbiota, and their relationship. The results showed that FF exposure caused significant changes in gut morphology, immune responses, apoptosis characteristics, and antioxidant enzyme activities. The high concentration of FF also led to a decrease in microbial diversity and changes in composition. These findings provide new insights into the impact of antibiotics on gut health and microbiota in aquatic invertebrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Tong Li, Demin Zhang, Yunfei Sun, Xiaozhen Yang, Wenwen Xiao, Yongxu Cheng, Zhiyuan Yao
Summary: This study used 16S rRNA gene amplicon sequencing to investigate the effects of short-term probiotic application on the intestinal microbial communities of Chinese mitten crab (CMC), revealing poor probiotic colonization in CMC intestines, no significant impact on alpha-diversity, significant changes in beta-diversity, and promotion of butanoate metabolism in CMC intestinal microbiota.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Xuran Liu, Wenlong Wan, Mengge Li, Jiayuan Shi, Jie Xu, Zihan Zhou, Anran Wang, Shuyan Miao
Summary: This study investigated the effects of dietary niacin on the growth performance, intestinal histomorphology, body composition, and antioxidant capacity of juvenile Eriocheir sinensis. The results showed that appropriate dietary niacin improved the growth performance and intestinal histomorphology of the crabs, and enhanced their antioxidant capacity.
AQUACULTURE NUTRITION
(2022)
Article
Biotechnology & Applied Microbiology
Hongli Liu, Yang Feng, Ma Yang, Ya Huang, Minghao Li, Yi Geng, Ping Ouyang, Defang Chen, Shiyong Yang, Lizi Yin, Liangyu Li, Xiaoli Huang
Summary: Research reveals that starvation leads to a decrease in the hepatopancreas index of Chinese mitten crab, affecting tissue structure, metabolism, and angiogenesis. The AKT signaling pathway plays a crucial role in this process, regulating gene expression related to angiogenesis and apoptosis, ultimately causing hepatopancreas atrophy.
Article
Chemistry, Applied
Jiada Lu, Hongwei Luan, Change Wang, Lili Zhang, Wenzheng Shi, Shuang Xu, Yinzhe Jin, Ying Lu
Summary: This study discovered that hemocyanin in crustaceans is allergenic to humans, but there is limited information about its molecular, structural, and allergenic properties. The purified natural protein was identified as Eriocheir sinensis HC by LC-MS/MS, and it showed allergenicity by reacting with serum IgE from crustacean patients. Molecular property analysis revealed that HC is resistant to trypsin digestion, but not heat-stable. Boiling (55.05 ± 3.50%) and steaming (66.84 ± 1.65%) increased β-sheet content and decreased the allergenicity of HC. By comparing the amino acid sequences of eight crustaceans, HC was found to be highly conserved. Five epitopes of HC were identified and validated using a murine sensitization model, with two of them (P3 and P10) matching the predictions from six types of bioinformatics analysis. Combining multiple bioinformatics analyses with a murine sensitization model appears to be an effective method for identifying allergenic epitopes.
Article
Fisheries
Yufan Bao, Guoqing Shen, Yanan Guo, Qun Wang, Xinpeng Fan, Weiwei Li
Summary: By studying the TNF of Eriocheir sinensis, it was found that TNF binding TNFR can affect the proliferation of hemocytes, possibly by regulating the expression of some proliferation-related genes.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Baoli Zhang, Changyue Yu, Yingkai Xu, Ziwei Huang, Yuqiao Cai, Yingdong Li
Summary: Photoperiod plays a vital role in the growth, development, and metabolism of crustaceans. The study revealed that the photoperiod affects the gene expression in the hepatopancreas of Chinese mitten crab, leading to changes in immune mechanisms. High-throughput Illumina sequencing was used to explore the genes associated with immune function, and the results demonstrated the impact of photoperiod on gene expression patterns.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Ju Li, Yuxin Tian, Xiaohong Li, Jinmei Zuo, Ruihao Zhao, Jinsheng Sun
Summary: Limb autotomy has a significant impact on the culture of Chinese mitten crabs. The insulin-like signaling pathway plays a key role in limb regeneration of these crabs. Inhibition of this pathway may reduce limb regeneration rate and the size of newly formed limbs.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Bingchan Jia, Yifan Li, Xinyuan Zi, Xiaohong Gu, Hezhong Yuan, Erik Jeppesen, Qingfei Zeng
Summary: Microbial communities play a critical role in aquaculture ecosystems. This study investigates the influence of sediment nutrient levels on microbial communities in Chinese mitten crab culture ponds. The results show that the diversity and composition of microbial communities vary significantly with different levels of sediment nutrient enrichment. Total nitrogen, organic matter, and pH are identified as important factors driving sediment bacterial community aggregation.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Zhigang Yang, Yucheng Fang, Jiani Liu, Aqin Chen, Yongxu Cheng, Youji Wang
Summary: The study found that moderate acidification has a positive effect on the defense of Chinese mitten crabs against the harmful substance phenanthrene. Phenanthrene significantly inhibits the antioxidant capacity of crabs, but moderate acidification can alleviate this toxic effect.
Article
Environmental Sciences
Yuzhen Luo, Weiwei Zhang, Yaying Li, Yongjie Wang, Huaiying Yao, Jigang Han
JOURNAL OF SOILS AND SEDIMENTS
(2020)
Article
Fisheries
Wenxu Xia, Xiaobing Chen, Shuling Yan, Yongjie Wang
AQUACULTURE RESEARCH
(2020)
Article
Microbiology
Xiaosha Liang, Liang Zhou, Shuling Yan, Yongjie Wang
ARCHIVES OF MICROBIOLOGY
(2020)
Correction
Microbiology
Xiaosha Liang, Liang Zhou, Shuling Yan, Yongjie Wang
ARCHIVES OF MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Shuang Wu, Liang Zhou, Yifan Zhou, Hongming Wang, Jinzhou Xiao, Shuling Yan, Yongjie Wang
Article
Microbiology
Yuri Wolf, Sukrit Silas, Yongjie Wang, Shuang Wu, Michael Bocek, Darius Kazlauskas, Mart Krupovic, Andrew Fire, Valerian V. Dolja, Eugene Koonin
NATURE MICROBIOLOGY
(2020)
Article
Virology
Tianhui Jia, Yongxin Yu, Yongjie Wang
ARCHIVES OF VIROLOGY
(2020)
Article
Microbiology
Mingshu Yang, Feng Han, Yongxin Yu, Yongjie Wang
Summary: Bacterial strain ODT-83 isolated from oysters can adsorb norovirus via HBGA-like substances. The complete genome sequence of ODT-83 reveals it as a new Pseudomonas oleovorans strain with 5037 predicted ORFs, 66 tRNA genes, and 12 rRNA genes, along with gene clusters for synthesizing alginate and Pel polysaccharides. These findings set the stage for further research on the interaction between ODT-83 and NoV.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Mingshu Yang, Yongxin Yu, Yongjie Wang
Summary: A novel F-specific RNA bacteriophage, YM1, isolated from human stool samples, belongs to genogroup I of Levivirus. Its complete genomic sequence is 3551 nt in length and shows high host specificity towards E. coli strains. With a diameter of 23 nm, YM1 may serve as a candidate for monitoring norovirus contamination in the environments.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Fisheries
Shuaiming Bao, Shuangshuang Gao, Mengmeng Zhang, Yongjie Wang
Summary: The study showed that PirAB(vc)-like protein did not induce AHPND symptoms or death in shrimp, and its structure and function are similar to PirA and PirB proteins. However, subtle differences may affect ligand binding and binary protein complex formation.
JOURNAL OF FISH DISEASES
(2022)
Article
Virology
Yijian Sheng, Zhenqi Wu, Shengzhong Xu, Yongjie Wang
Summary: In this study, a novel tripartite interaction system consisting of micro-green alga (Chlorella sp.), Mimiviridae large green alga virus, and virophage was successfully discovered and identified at the coculture level, with Chlorella sp. as the eukaryotic host. The large green alga virus CV-XW01 is closely related to the Mimiviridae giant virus Cafeteria roenbergensis virus, host virus of the virophage Mavirus, as well as a novel large virus of Mimiviridae that infects a non-protozoan protist host. The virophage CVv-SW01 highly resembles Mavirus in its codon usage frequency and preference, although they are phylogenetically distantly related. These findings provide novel insights into the diversity of large/giant viruses and their virophages.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Yifan Zhou, Liang Zhou, Shuling Yan, Lanming Chen, Mart Krupovic, Yongjie Wang
Summary: This study assessed the utility of CRISPR spacer targeting, tRNA gene matching, and homology searches for the assignment of archaeal hosts of viruses and validated these approaches on metaviromes from Yangshan Harbor. The results showed a high diversity of archaeal viruses in the YSH virome and proposed three new viral families for the classification of marine archaeal viruses. The study highlights the importance of viral metagenomics in exploring the archaeal virome and provides new insights into the diversity, distribution, and evolution of marine archaeal viruses.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Virology
Fangxin Lu, Shuang Wu, Yimin Ni, Yongxin Yu, Songzhe Fu, Yongjie Wang
Summary: In this study, the complete genome sequence of a new RNA virus, named marine RNA virus Yangshan-LWW (YS-LWW), was obtained from Yangshan Harbor. Further analysis revealed that YS-LWW belongs to a new species in the genus Locarnavirus and is widely detected in coastal and freshwater environments, suggesting its potential significance in aquatic ecosystems.
ARCHIVES OF VIROLOGY
(2023)
Article
Virology
Zhenqi Wu, Ting Chu, Yijian Sheng, Yongxin Yu, Yongjie Wang
Summary: Virophages, small double-stranded DNA viruses that rely on the viral factory of large host viruses, are found abundantly in global marine environments. Analysis of the Global Ocean Viromes 2.0 dataset revealed 94 virophage sequences, including 8 complete genomes. Additionally, 45 large virus sequences were identified, possibly involved in cell-virus-virophage trisome relationships.
Article
Virology
Haoyun Cai, Yifan Zhou, Xiefei Li, Tianqi Xu, Yimin Ni, Shuang Wu, Yongxin Yu, Yongjie Wang
Summary: Seven uncultured dsDNA bacteriophages were found in Dishui Lake, Shanghai, and their whole genomes were analyzed. The phages showed a close association with protein synthesis and host metabolism. Phylogenetic analysis revealed the presence of three new genera within two known viral families, and four new families in Caudoviricetes.