Article
Immunology
Philip Nikolic, Poonam Mudgil, David G. Harman, John Whitehall
Summary: This study aimed to identify and compare the general differences in protein profiles among clinical strains of S. aureus sensitive and resistant to methicillin. The proteomic analysis revealed differences in protein abundances related to virulence, metabolism, and protein synthesis between MSSA and MRSA strains.
MICROBIAL PATHOGENESIS
(2023)
Article
Public, Environmental & Occupational Health
Sara Quero, Marina Serras-Pujol, Noemi Parraga-Nino, Carmen Torres, Marian Navarro, Anna Vilamala, Emma Puigoriol, Javier Diez de los Rios, Elisenda Arque, Judit Serra-Pladevall, Alba Romero, Daniel Molina, Roger Paredes, Maria Luisa Pedro-Botet, Esteban Reynaga
Summary: This study investigated the prevalence of methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) among workers in the pork production chain. The results showed that the prevalence of MRSA was high among workers in contact with live animals, especially in pig farms, livestock transporters, and slaughterhouses.
Article
Genetics & Heredity
Hongbin Chen, Yuyao Yin, Lucy van Dorp, Liam P. Shaw, Hua Gao, Mislav Acman, Jizhen Yuan, Fengning Chen, Shijun Sun, Xiaojuan Wang, Shuguang Li, Yawei Zhang, Rhys A. Farrer, Hui Wang, Francois Balloux
Summary: The study reveals that in China, the community-associated MRSA strain ST59 is gradually replacing the healthcare-associated strain ST239, mainly due to its higher virulence potential rather than antibiotic resistance or biofilm production. The chemotaxis inhibitory protein (chp) carried by ST59 may be a significant factor contributing to its increased virulence.
Article
Biotechnology & Applied Microbiology
Han Young Chung, You-Tae Kim, Joon-Gi Kwon, Han Hyeok Im, Duhyun Ko, Ju-Hoon Lee, Sang Ho Choi
Summary: The study identified a MRSA strain that may originate from animal foods, with contamination leading to human infections. Transcriptome analysis revealed specific regulation mechanisms for obtaining amino acids from raw chicken breast by this MRSA strain. Additionally, upregulation of toxin gene expressions increased the risk of infection through contaminated food.
Article
Biotechnology & Applied Microbiology
Santosh Paudel, Kamal Bagale, Swapnil Patel, Nicholas J. Kooyers, Ritwij Kulkarni
Summary: Human urine significantly alters the physiology and gene expression of MRSA, aiding the bacterium in adapting to the nutrient-limiting and immunologically hostile conditions within the urinary tract to facilitate UTI.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Soo Tein Ngoi, Wen Kiong Niek, Yee Wan Lee, Sazaly AbuBakar, Cindy Shuan Ju Teh
Summary: This study investigates the genomic features of a highly virulent yet pan-susceptible MSSA strain known as HS-MSSA, which caused a fatal sepsis in a dengue patient. The analysis revealed a novel combined genotype and the presence of multiple pathogenic genes in this strain, highlighting its high virulence potential.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Maged El-Ashker, Stefan Monecke, Mayada Gwida, Thoraya Saad, Adel El-Gohary, Amro Mohamed, Annett Reissig, Katrin Frankenfeld, Dominik Gary, Elke Mueller, Ralf Ehricht
Summary: This study aims to investigate the clonal diversity of Staphylococcus aureus strains in healthy dairy cattle, buffaloes, and their caretakers in the Nile Delta region of Egypt. The results reveal a high clonal diversity of multi-drug resistant S. aureus clones that colonize the animals and caretakers, indicating a potential health threat for both populations.
VETERINARY MICROBIOLOGY
(2022)
Review
Infectious Diseases
Joshua. B. Parsons, Annette. C. Westgeest, Brian. P. B. Conlon, Vance. G. Fowler
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a deadly pathogen that can persist in the bloodstream for days despite appropriate antibiotics. Persistent MRSA bacteremia is common and associated with poor clinical outcomes. This review explores the factors related to host-pathogen interaction and discusses the clinical relevance of each element. Treatment options and diagnostic approaches for managing persistent MRSA bacteremia are also discussed.
Article
Infectious Diseases
Zahoua Mentfakh Laceb, Seydina M. Diene, Rym Lalaoui, Mabrouk Kihal, Fella Hamaidi Chergui, Jean-Marc Rolain, Linda Hadjadj
Summary: This study determined the prevalence of Staphylococcus aureus in a hospital environment in western Algeria and investigated nasal carriage among healthcare workers and patients. The study found a high prevalence of S. aureus in all three groups, as well as the presence of resistance and virulence genes. It also identified the hospital environment and nasal carriage as potential reservoirs for the persistence and spread of the same pathogenic clone.
Article
Immunology
Dan Yang, Shuang Wang, Erchao Sun, Yibao Chen, Lin Hua, Xiangru Wang, Rui Zhou, Huanchun Chen, Zhong Peng, Bin Wu
Summary: This study isolated a temperate bacteriophage vB_Saus_PHB21 and found that its integration into the host MRSA increased the host's capacities of cell adhesion, anti-phagocytosis, and biofilm formation, leading to severe mortalities in infection.
Article
Microbiology
Yanath Belguesmia, Giuseppe Spano, Djamel Drider
Summary: This study demonstrated the sensitivity of clinical MRSA-S1 strain to a combination of the bacteriocin EntDD14 and the antibiotic methicillin. The combination effectively reduced biofilm formation and downregulated virulence genes, suggesting the potential use of bacteriocins and antibiotics in treating bacterial infections.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Martyna Kasela, Agnieszka Grzegorczyk, Boiena Nowakowicz-Debek, Anna Malm
Summary: The study investigated the molecular and phenotypic characteristics of 21 MRSA collected from a nursing home in Lublin, Poland in 2018, identifying a low prevalence of multidrug-resistant MRSA strains. Furthermore, the emergence of novel MRSA strains with unique virulence and the presence of the epidemic clone EMRSA-15 present challenges for controlling the spread of MRSA in nursing homes.
Article
Biochemistry & Molecular Biology
Mark Hutchins, Richard A. Bovill, Peter J. Stephens, John A. Brazier, Helen M. I. Osborn
Summary: The increasing number of antibiotic-resistant bacteria poses a serious threat to global human health. This study examines the glycosylation of nadifloxacin to improve its solubility and finds that one glycoside derivative has the potential to be an effective antibacterial agent.
Article
Infectious Diseases
Hanzada T. Nour El-Din, Aymen S. Yassin, Yasser M. Ragab, Abdelgawad M. Hashenn
Summary: In this study, it was found that infectious MRSA isolates showed a higher tendency for biofilm formation and borderline minimum inhibitory concentrations. A positive correlation was observed between susceptibility to protein synthesis inhibitors and cell wall inhibitors. Additionally, nasal samples were enriched in rifampicin-resistant isolates, while urine and blood samples were enriched in susceptible ones.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Thomas H. McConville, Eloise D. Austin, Wenjing Geng, Qiuhu Shi, Divya Balasubramanian, Christine J. Kubin, Victor J. Torres, Anne-Catrin Uhlemann
Summary: This study investigated the association between cytotoxicity and 30-day mortality in patients with Methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infections. The results showed no significant association between low cytotoxic activity and poor outcomes. Different genotypes and primary infection sites may affect cytotoxicity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Vladimir Gostev, Olga Kalinogorskaya, Julia Sopova, Ofelia Sulian, Polina Chulkova, Maria Velizhanina, Irina Tsvetkova, Irina Ageevets, Vladimir Ageevets, Sergey Sidorenko
Summary: Vancomycin and daptomycin are commonly used antibiotics for treating methicillin-resistant Staphylococcus aureus infections, but their efficacy is limited by their resistance and associated resistance to each other. The effectiveness of novel lipoglycopeptides in overcoming this associated resistance is still unknown.
Article
Infectious Diseases
Vladimir Gostev, Ksenia Sabinova, Julia Sopova, Olga Kalinogorskaya, Ofeliia Sulian, Polina Chulkova, Maria Velizhanina, Polina Pavlova, Lavrentii Danilov, Lyudmila Kraeva, Dmitrii Polev, Elvira Martens, Sergey Sidorenko
Summary: The aim of this study was to investigate the phenotypic and genetic properties of oxacillin-susceptible methicillin-resistant Staphylococcus aureus (OS-MRSA) isolates and their beta-lactam resistant derivatives. The findings showed that OS-MRSA isolates were more prevalent among community-acquired MRSA than hospital-acquired MRSA. They were characterized by specific genetic mutations and lower levels of mecA expression compared to control MRSA isolates. The study emphasized the importance of accurate detection and appropriate treatment of OS-MRSA in order to prevent resistance development and treatment failures.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Kamilla B. Alieva, Maria N. Golikova, Svetlana A. Dovzhenko, Mikhail A. Kobrin, Elena S. Strukova, Vladimir V. Ageevets, Alisa H. Avdeeva, Ofeliia Sulian, Sergey Sidorenko, Stephen Zinner
Summary: OXA-48 carbapenemases are frequently expressed by Klebsiella pneumoniae clinical isolates and decrease the effectiveness of meropenem therapy. The emergence of resistance in susceptible carbapenemase-producing isolates and their resistance patterns when exposed to meropenem are not completely understood. This study confirmed the applicability of the mutant selection window (MSW) hypothesis to meropenem and carbapenemase-producing K. pneumoniae.
Article
Medicine, General & Internal
Ivan Evdokimov, Evgeny Papynov, Oleg Shichalin, Igor Afonin, Vladimir Apanasevich, Valeriy Tolmachev, Yuriy Krasnikov, Yuliya Gainullina, Galina Reva, Ivan Reva, Viktor Uso
Summary: The purpose of this study was to determine the regeneration ability of bone tissue using modern bioceramics synthesized through reactive spark plasma sintering. The study involved 26 female New Zealand White rabbits divided into two groups. X-ray densitometry was used to evaluate the regeneration of burr hole defects covered with bioceramic implants on days 7, 40, and 210 after surgery. The results showed tight fixation of the implants without dislocation, absence of osteoporosis and osteolysis in surrounding tissues, and the formation of dense bone tissue on the 210th day after surgery. The use of bioceramic implants allows for immediate closure of large bone defects and stimulation of dense bone formation.
Article
Medicine, General & Internal
Ivan Reva, Tatsuo Yamamoto, Ellada Slabenko, Marina Fleishman, Olga Lebedko, Mariya Tuchina, Viktoriya Semiglasova, Yuriy Krasnikov, Valeriy Tolmachev, Yuliya Gainullina, Galina Reva
Summary: The investigation of red bone marrow samples from deceased COVID-19 patients identified secondary hemophagocytosis. Phagocytic reactions in SARS-CoV-2-infected patients were found to affect both erythrocytes and leukocytes. These findings expand the understanding of COVID-19 pathogenesis and suggest new therapeutic measures to address the involvement of young erythrocytes and lymphocytes in the red bone marrow in the cascade of pathological reactions. The study also highlights the wide range of damaging effects of SARS-CoV-2 and its impact on multiple organ failure.
Article
Medicine, General & Internal
Ivan Reva, Tatsuo Yamamoto, Kirill Stegniy, Viktor Usov, Alexander Zolotov, Anatoliy Soroka, Natalya Struzkina, Ellada Slabenko, Pavel Zhibanov, Kseniya Porva, Galina Reva
Summary: The frequency of complications associated with cosmetic invasive interventions varies with age, gender, and location of skin lesions. In men, injuries are often related to burn injuries on the extremities, while complications of facial skin regeneration are more common in women aged 35 to 65 years, often due to the use of low-quality materials or a history of keloid scars.
Article
Medicine, General & Internal
Ivan Reva, Tatsuo Yamamoto, Kirill Stegniy, Viktor Usov, Alexander Zolotov, Anatoliy Soroka, Natalya Struzkina, Ellada Slabenko, Pavel Zhibanov, Kseniya Porva, Galina Reva
Summary: Pathological scarring lacks a clear explanation in terms of its cause, and it is characterized by the unpredictable and continuous growth of keloid scars, which do not regress for many years and are difficult to treat effectively. Research shows that the number of macrophages in keloid scars is reduced compared to areas of normal skin regeneration and hypertrophic scars, which may contribute to keloid scarring and connective tissue transformation.
Article
Biochemistry & Molecular Biology
O. E. Khokhlova, I. V. Vladimirov, R. S. Kozlov, I. V. Lazareva, M. V. Edelstein, I. A. Larionova, A. V. Molodtsova, V. A. Avdeeva, N. K. Fursova, S. V. Sidorenko
Summary: This study investigates the mechanisms of antibiotic resistance in pathogens causing pyroinflammatory complications in burn-injury patients. The findings reveal a diverse distribution of resistant strains, some of which carry multiple resistance genes.
MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
(2022)
Article
Medicine, General & Internal
Dmitrii Puga, Ivan Reva, Tatsuo Yamamoto, Svetlana Nikolaenko, Grachik Abramyan, Marina Fleishman, Anastasia Shindina, Konstantin Lonshakov, Tatiana Lemeshko, Anatoliy Soroka, Galina Reva
Summary: Using immunohistochemistry, this study explored the features of gender hormone receptors in the structure of the cervix mucous membrane epithelium in postmenopausal women and their relationship with keratinocyte proliferative activity. It demonstrated the significance of estrogen deficiency in proliferative activity and identified potential therapeutic targets for regulating and preventing neoplasia and malignancy in the cervical epithelium structure.
Article
Medicine, General & Internal
Ivan Reva, Tatsuo Yamamoto, Grachik Abramyan, Marina Fleishman, Olga Lebedko, Anastasia Shindina, Konstantin Lonshakov, Tatiana Lemeshko, Anatoliy Soroka, Galina Reva, Dmitriy Puga
Summary: This article analyzes the changes in immune cells and their quantity in the cervical mucous membrane of postmenopausal women using immunohistochemistry, and finds differences in immune representation in different parts of the mucous membrane.
Article
Medicine, General & Internal
Ivan Reva, Tatsuo Yamamoto, Kirill Stegniy, Ellada Slabenko, Viktoriya Semiglasova, Igor Sementsov, Olga Lebed'ko, Marina Fleishman, Aleksey Kudelya, Mariya Tuchina, Viktor Usov, Yuriy Krasnikov, Ekaterina Mozhilevskaya, Tatiana Lemeshko, Aleksey Novikov, Valeriy Tolmachev, Galina Reva
Summary: With the emergence of new SARS-CoV-2 strains, studying the cellular damage caused by the virus is crucial for vaccine development and treatment strategies. Research has shown that the red bone marrow of COVID-19 patients exhibits signs of stromal and parenchymal damage, as well as pathologic damage to erythropoiesis, providing valuable insights for targeted therapy.
Article
Medicine, General & Internal
Ivan Reva, Kirill Stegniy, Ekaterina Dvoinikova, Ekaterina Mozhilevskaya, Marina Fleischman, Stanislav Ichenko, Valeriy Tolmachev, Igor Sementsov, Ellada Slabenko, Dmitriy Zvyagintsev, Rodion Gorbarenko, Aleksandr Zolotov, Tamara Obydennikova, Galina Reva
Summary: The development of targeted treatment methods for inhibiting angiogenesis in malignant tissues is crucial for modern oncology. This study focuses on the expression characteristics of CD34-positive cells at the border of the tumor and malignant tissue. High expression of CD34-positive cells in small and large vessels correlates with tumor size and disease age during malignancy. VEGF, the main regulator of angiogenesis during tissue malignancy, presents a promising target for targeted treatment.
Article
Medicine, General & Internal
Evgeniy Martynenko, Tamara Obydennikova, Kirill Stegniy, Ivan Reva, Marina Fleischman, Aleksandr Zolotov, Dmitriy Zvyagintsev, Sergey Terekhov, Olga Lebedko, Igor Sementsov, Ellada Slabenko, Galina Reva, Viktor Usov
Summary: This study aimed to optimize the timing of autodermoplasty in the treatment of burns by developing morphological criteria. The results showed that the optimal time for autodermoplasty is 7-8 days after thermal injury, while transplantation after 14 days is less effective.
