Article
Biochemistry & Molecular Biology
Kejia Kan, Yifei Mu, Marielle Bouschbacher, Carsten Sticht, Natalia Kuch, Martin Sigl, Nuh Rahbari, Norbert Gretz, Prama Pallavi, Michael Keese
Summary: The study revealed a novel biphasic response of human umbilical vein endothelial cells to blue light illumination. Low fluence blue light promoted cell activities, while high fluence illumination had the opposite effect by upregulating pro-apoptotic signaling pathways. These findings provide insights into photobiomodulation by blue light and potential treatment strategies for angiogenesis-dependent diseases.
Article
Chemistry, Medicinal
Chin-Feng Hsuan, Thung-Lip Lee, Wei-Kung Tseng, Chau-Chung Wu, Chi-Chang Chang, Tsui-Ling Ko, Ya-Ling Chen, Jer-Yiing Houng
Summary: The study demonstrated that extracts from Glossogyne tenuifolia (GT) can significantly increase nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs), with the root methanol extract showing the most potent effect. The root methanol extract increased NO production by increasing eNOS expression and activating eNOS phosphorylation at serine(1177).
Article
Food Science & Technology
Ho-Jeong Bang, Mok-Ryeon Ahn
Summary: This study found that pinobanksin exhibits antiangiogenic effect by inducing endothelial cell apoptosis, and it can be developed as an antiangiogenic agent. The mechanism involves inactivation of the Raf/MEK/ERK pathway and activation of apoptotic signaling such as caspase cascades.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biochemistry & Molecular Biology
Thomas Jansen, Miroslava Kvandova, Isabella Schmal, Sanela Kalinovic, Paul Stamm, Marin Kuntic, Marc Foretz, Benoit Viollet, Andreas Daiber, Matthias Oelze, John F. Keaney Jr, Thomas Muenzel, Eberhard Schulz, Swenja Kroeller-Schoen
Summary: The study demonstrates that mice with endothelial-specific deletion of alpha 1AMPK have detrimental effects during voluntary exercise training, including increased reactive oxygen species production, eNOS uncoupling, and endothelial dysfunction. These results suggest that endothelial alpha 1AMPK plays a critical role in vascular protection and determines whether the effects of exercise on the vasculature are beneficial or harmful.
Article
Environmental Sciences
Jiajing Cui, Pingwei Wang, Shuqi Yan, Yujun Liang, Dongge Liu, Shuping Ren
Summary: The study aims to explore the role of ferroptosis in endothelial cell dysfunction and its underlying mechanisms.
Article
Endocrinology & Metabolism
Maria Krajewska, Ewelina Witkowska-Sedek, Malgorzata Ruminska, Anna M. Kucharska, Anna Stelmaszczyk-Emmel, Maria Sobol, Anna Majcher, Beata Pyrzak
Summary: This study aimed to investigate the relationship between vitamin D, chemerin, and metabolic profile in overweight and obese children. The results showed that vitamin D supplementation led to improvements in CRP, total cholesterol, ALT, and OGTT 150-minute glucose levels. Additionally, there was a tendency for a negative association between 25(OH)D and chemerin levels.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wenxue Ma, Tingting Zhong, Junqiu Chen, Xiao Ke, Huihua Zuo, Qiang Liu
Summary: This study aimed to investigate the effect of exogenous hydrogen sulfide (H2S) on high glucose-induced injury in endothelial progenitor cells (EPCs) and its underlying mechanisms. The results showed that H2S reversed the inhibitory effects of high glucose on EPCs proliferation, migration, and tube formation, promoting autophagic flux and reducing reactive oxygen species (ROS) production.
Article
Pharmacology & Pharmacy
Wei Liu, Yanghui Ou, Yumeng Yang, Xuemei Zhang, Liqi Huang, Xiaohua Wang, Buling Wu, Mingcheng Huang
Summary: The study demonstrated that punicalagin has a protective effect on HUVECs activation through an endothelial-mediated mechanism, which may be used in the treatment of various disorders such as cancer, rheumatoid arthritis, and cardiovascular disease. This effect is achieved by reducing adhesion of monocyte cells to HUVECs, decreasing expression of ICAM-1 and VCAM-1, and inhibiting permeability, proliferation, migration, and tube formation in VEGF-induced HUVECs. Additionally, punicalagin suppresses IKK-mediated activation of NF-kappa B signaling and inhibits VEGFR2 activation and downstream p-PAK1.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Hematology
Fahim Abbasi, Cindy Lamendola, Chelsea S. Harris, Vander Harris, Ming-Shian Tsai, Pragya Tripathi, Fakhar Abbas, Gerald M. Reaven, Peter D. Reaven, Michael P. Snyder, Sun H. Kim, Joshua W. Knowles
Summary: The study showed that high-intensity atorvastatin for 10 weeks increased insulin resistance and insulin secretion in individuals without type 2 diabetes, potentially leading to an increased risk of developing diabetes over time.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lukasz Gwozdzinski, Joanna Bernasinska-Slomczewska, Pawel Hikisz, Anna Wiktorowska-Owczarek, Edward Kowalczyk, Anna Pieniazek
Summary: In this study, the properties of human varicose vein endothelial cells (HVVEC) and human umbilical vein endothelial cells (HUVEC) were compared. Three bioactive compounds, diosmin, escin, and bromelain, were tested on the cells. The drugs showed different effects on reactive oxygen species and nitric oxide generation in the cells. The study revealed a potential mechanism of action for VV therapy drugs on HVVEC cells.
Article
Plant Sciences
Ji Hye Chun, Melissa M. Henckel, Leslie A. Knaub, Sara E. Hull, Greg B. Pott, David G. Ramirez, Jane E. -B. Reusch, Amy C. Keller
Summary: The study found that (-)-epicatechin can alleviate glucose intolerance induced by thermoneutral housing, and improve carbohydrate tolerance through increased insulin secretion.
Article
Nutrition & Dietetics
Ruihao Wu, Jiayin Zhong, Lei Song, Min Zhang, Lulu Chen, Li Zhang, Zhaohui Qiu
Summary: In this study, untargeted metabolomics was used to examine differentially expressed metabolites of vascular endothelial cells, aiming to understand the metabolic regulation of ischemic injury. Various methods including CCK8 detection, flow cytometry, ROS detection, JC-1 detection, and western blotting were employed to assess the effects of differentially expressed metabolites on apoptosis and oxidative stress in cells. The impacted metabolic pathways were further confirmed by western blotting and RT-PCR.
NUTRITION & METABOLISM
(2023)
Article
Genetics & Heredity
Regina L. Binz, Rupak Pathak
Summary: This study investigated the cytogenetic integrity of primary human umbilical vein endothelial cells (HUVECs) and found that approximately 50% of the cells have a deletion of the Xq terminal region. Additionally, a similar Xq deletion was identified in the daughter cell line generated by fusing HUVECs with A549 cells.
Article
Biochemistry & Molecular Biology
Spela Volcansek, Urska Rahne Perc, Mojca Lunder, Drazenka Pongrac Barlovic
Summary: Reactive postprandial hypoglycemia (RPH) is an understudied condition that lacks clinical definition and knowledge of future health implications. This study aimed to assess the glycemic response after glucose ingestion in individuals with RPH and compare it with healthy volunteers. The results showed that RPH subjects did not exhibit deteriorated insulin sensitivity or insulin resistance, and there were no significant differences in glucose-insulin curves during oral glucose tolerance tests compared to healthy controls.
Article
Integrative & Complementary Medicine
Ke Shi-ye, Yu Shu-jie, Liu Ding-hui, Shi Guang-yao, Wang Min, Zhou Bin, Wu Lin, Song Zhi-ming, Zhu Jie-ming, Wu Chao-dong, Qian Xiao-xian
Summary: The results showed that Rb1 can protect HUVECs from high glucose-induced apoptosis by promoting mitochondrial function and suppressing oxidative stress through the SIRT3 signaling pathway.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jaime A. Gutierrez, Isabel Gomez, Delia Chiarello, Rocio Salsoso, Andres D. Klein, Enrique Guzman-Gutierrez, Fernando Toledo, Luis Sobrevia
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Review
Biochemistry & Molecular Biology
Victoria Gallardo, Marcelo Gonzalez, Fernando Toledo, Luis Sobrevia
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Susana Rojas, Emerita Basualto, Luz Valdivia, Natalia Vallejos, Karen Ceballos, Eduardo Pena, Coralia Rivas, Francisco Nualart, Enrique Guzman-Gutierrez, Carlos Escudero, Fernando Toledo, Luis Sobrevia, Marcela Cid, Marcelo Gonzalez
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Miguel Merino, Sebastian San Martin, Pedro Sandana, Kurt Herlitz, Claudio Aguayo, Alejandro Godoy, Pablo Torres-Vergara, Marcelo Gonzalez, Felipe Troncoso, Jesenia Acurio, Carlos Escudero
PURINERGIC SIGNALLING
(2020)
Article
Developmental Biology
Juan Araya, Andres Rodriguez, Karin Lagos-SanMartin, Daniela Mennickent, Sebastian Gutierrez-Vega, Bernel Ortega-Contreras, Barbara Valderrama-Gutierrez, Marcelo Gonzalez, Marcelo Farias-Jofre, Enrique Guzman-Gutierrez
Summary: This study utilized machine learning to identify a potential correlation between maternal thyroid profile and gestational diabetes mellitus (GDM) during the first and second trimesters of pregnancy, suggesting that this correlation could serve as a complementary tool for a more sensitive GDM diagnosis.
Article
Multidisciplinary Sciences
Sebastian Gutierrez-Vega, Axel Armella, Daniela Mennickent, Marco Loyola, Ambart Covarrubias, Bernel Ortega-Contreras, Carlos Escudero, Marcelo Gonzalez, Martin Alcala, Maria del Pilar Ramos, Marta Viana, Erica Castro, Andrea Leiva, Enrique Guzman-Gutierrez
Article
Multidisciplinary Sciences
J. Tapia, N. Vera, Joao Aguilar, M. Gonzalez, S. A. Sanchez, P. Coelho, C. Saavedra, J. Staforelli
Summary: Using dual time resolved membrane fluctuation spectroscopy, it was found that human red blood cell membrane fluctuation is dependent on d-glucose concentration, with mechanical parameters changing accordingly. Under different d-glucose concentrations, an increase in the fluctuation amplitude of the lipid bilayer was observed, along with a decrease in tension value, bending modulus, and fluidity.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Soumyalekshmi Nair, Dominic Guanzon, Nanthini Jayabalan, Andrew Lai, Katherin Scholz-Romero, Priyakshi Kalita de Croft, Valeska Ormazabal, Carlos Palma, Emilio Diaz, Elizabeth A. McCarthy, Alexis Shub, Jezid Miranda, Eduard Gratacos, Fatima Crispi, Gregory Duncombe, Martha Lappas, H. David McIntyre, Gregory Rice, Carlos Salomon
Summary: Gestational diabetes mellitus (GDM) poses a significant public health concern, affecting 9-15% of pregnancies worldwide. This study found that EV-associated miRNAs in GDM patients undergo significant changes during gestation and can be effectively used for classification. A set of proteins associated with JAK-STAT signaling in skeletal muscle biopsies from GDM patients was identified, which may be targeted by the miRNA-92a-3p carried by circulating EVs. Overexpression of miRNA-92a-3p was shown to increase insulin sensitivity in primary skeletal muscle cells.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Carlos Palma, H. David McIntyre, Carlos Salomon
Summary: Research on extracellular vesicles (EVs) has provided insights into physiological and pathophysiological conditions, particularly during pregnancy. Gestational diabetes mellitus (GDM) is a common and influential complication that affects the miRNAs and proteins within EVs, leading to metabolic changes.
Review
Physiology
Paola Ayala-Ramirez, Marcelo Gonzalez, Carlos Escudero, Laura Quintero-Arciniegas, Fernanda R. Giachini, Raiany Alves de Freitas, Alicia E. Damiano, Reggie Garcia-Robles
Summary: COVID-19 infection during pregnancy can have negative effects on pregnant women, causing severe systemic inflammatory response, vascular alterations, and abnormal placental histology. However, the risks of administering COVID-19 vaccines during pregnancy appear to be minimal.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Camila Reyes, Estefania Nova-Lamperti, Daniel Duran-Sandoval, Daniela Rojas, Jorge Gajardo, Enrique Guzman-Gutierrez, Camila Bustos-Ruiz, Valeska Ormazabal, Felipe A. Zuniga, Carlos Escudero, Claudio Aguayo
Summary: This study demonstrates the anti-inflammatory effect and tissue damage reduction of LOXIN. Overexpression of LOXIN blocks Lox-1-mediated uptake of ox-LDL, resulting in decreased Lox-1 expression in endothelial cells and reduced fatty streak lesions in the aorta, as well as inflammation and fibrosis in the liver.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Constanza Sabando, Maite Rodriguez-Diaz, Walther Ide, Edgar Pastene, Marcia Avello, Mario Simirgiotis, Susana Rojas, Enrique Villarroel, Tiare Silva-Grecchi, Cristian Gutierrez, Rebeca Bouza, Barbara Cicchelli, Marcelo Gonzalez, Saddys Rodriguez-Llamazares
BIOLOGICAL RESEARCH
(2020)
Article
Endocrinology & Metabolism
Ramkumar Menon, Christopher Luke Dixon, Samantha Sheller-Miller, Stephen J. Fortunato, George R. Saade, Carlos Palma, Andrew Lai, Dominic Guanzon, Carlos Salomon