Article
Immunology
Nicole Prince, Su H. Chu, Yulu Chen, Kevin M. Mendez, Ellen Hanson, Leeanne Green-Snyder, Elizabeth Brooks, Susan Korrick, Jessica A. Lasky-Su, Rachel S. Kelly
Summary: Autism Spectrum Disorder (ASD) is a heterogeneous condition with a broad range of characteristics, and understanding the biology underlying phenotypic variability is important. This study analyzed the phenotype and metabolome of individuals with ASD to identify distinct subgroups and investigate their underlying biology. The results revealed unique metabolic patterns in each subgroup, which may contribute to specific ASD characteristics and have implications for personalized medicine approaches in managing ASD symptoms.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Biotechnology & Applied Microbiology
Shingo Noguchi, Kenichi Wakita, Fumio Matsuda, Hiroshi Shimizu
Summary: This study compared the metabolic phenotypes of cancer cells cultured in 2D and 3D environments using C-13 metabolic flux analysis. The results demonstrated distinct metabolic characteristics of cells cultured in 3D, showing significant differences in metabolic pathways compared to 2D cultures.
METABOLIC ENGINEERING
(2022)
Article
Immunology
Hyon-Xhi Tan, Jennifer A. Juno, Robyn Esterbauer, Hannah G. Kelly, Kathleen M. Wragg, Penny Konstandopoulos, Sheilajen Alcantara, Carolina Alvarado, Robert Jones, Graham Starkey, Boa Zhong Wang, Osamu Yoshino, Thomas Tiang, M. Lindsay Grayson, Helen Opdam, Rohit D'Costa, Angela Vago, Laura K. Mackay, Claire L. Gordon, David Masopust, Joanna R. Groom, Stephen J. Kent, Adam K. Wheatley
Summary: Recent studies have found that memory B cells can reside in inflamed tissues for a long time. This study characterized tissue-resident memory B cells in the lungs of mice after influenza infection and discovered that the mechanisms underlying their localization may be evolutionarily conserved.
SCIENCE IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sabine Groeger, Jens Martin Herrmann, Trinad Chakraborty, Eugen Domann, Sabine Ruf, Joerg Meyle
Summary: Periodontitis is a chronic inflammatory disease caused by a bacterial biofilm and is associated with cancer risk. Porphyromonas gingivalis is considered a keystone pathogen in periodontal biofilms. This study investigated the genome-wide impact of P. gingivalis W83 membranes on RNA expression of oral squamous carcinoma cells and found significant changes in cell metabolic pathways and replication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Daniel Winardi, Pei-Yi Chu, Guan-Yu Chen, Ke Wang, Wei-Yu Hsu, Ching-Liang Hsieh, Yung-Hsiang Chen, Yang-Chang Wu, Juan-Cheng Yang
Summary: Fangchinoline, a natural compound, is identified as a potential adjuvant for ovarian cancer therapy by inhibiting Aurora A kinase activity and enhancing the efficacy of cisplatin treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Gen Nishikawa, Kenji Kawada, Keita Hanada, Hisatsugu Maekawa, Yoshiro Itatani, Hiroyuki Miyoshi, Makoto Mark Taketo, Kazutaka Obama
Summary: Asparagine metabolism plays a critical role in cancer treatment, and inhibiting asparagine synthetase can effectively reduce tumor growth.
Article
Multidisciplinary Sciences
Nicholas S. Wilcox, Timur O. Yarovinsky, Prakruti Pandya, Vinod S. Ramgolam, Albertomaria Moro, Yinyu Wu, Stefania Nicoli, Karen K. Hirschi, Jeffrey R. Bender
Summary: Tissue resident macrophages largely originate from fetal liver and are long-lived, while bone marrow-derived macrophages are recruited after acute perturbations such as hypoxia in myocardial ischemia. This study identifies translational differences in gene expression programs between macrophage subsets in response to hypoxia, highlighting potential therapeutic targets in ischemic myocardium.
Article
Cell & Tissue Engineering
Axelle Bouche, Benoit Borner, Chloe Richard, Ysaline Grand, Didier Hannouche, Thomas Laumonier
Summary: This study investigated the heterogeneity of human MuRC and characterized their molecular signature and metabolic profile. The results showed two distinct subpopulations of MuRC distinguished by their relative Pax7 expression, with the Pax7High subpopulation being in a deeper quiescent state, less committed to differentiation, and displaying reduced metabolic activity. These findings suggest that human Pax7High MuRC may be a suitable stem cell source for potential therapeutic applications in skeletal muscle diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Oncology
Siriwat Sukphokkit, Pichamon Kiatwuthinon, Supeecha Kumkate, Tavan Janvilisri
Summary: This study established a 3D culture model of cholangiocarcinoma and found that 3D culture altered cell migration activity and galectin-3 expression, providing a more accurate model for studying the development of cholangiocarcinoma.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Benjamin A. Kansy, Tim P. Wehrs, Kirsten Bruderek, Yu Si, Sonja Ludwig, Freya Droege, Pia Hasskamp, Uta Henkel, Nina Dominas, Thomas K. Hoffmann, Peter A. Horn, Martin Schuler, Thomas C. Gauler, Monika Lindemann, Stephan Lang, Agnes Bankfalvi, Sven Brandau
Summary: Patients with HPV-related head and neck cancer have poorer outcomes after surgery and radiochemotherapy, possibly due to differences in immune status and immune activity. This study found differences in the frequency and ratio of MDSC and effector T cells between HPV+ and HPV- disease, providing new insights for more personalized immunotherapeutic approaches in the future.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Siddharth Shanbhag, Ahmad Rashad, Ellen Helgeland Nymark, Salwa Suliman, Catharina de Lange Davies, Andreas Stavropoulos, Anne Isine Bolstad, Kamal Mustafa
Summary: Cell coculture strategies using HUVEC and GPC spheroids in HPLG showed enhanced in vitro sprouting, suggesting a promising approach for promoting angiogenesis in tissue engineering constructs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell & Tissue Engineering
Sumin Cho, Hyemin Choi, Hyundoo Jeong, Su Yeon Kwon, Eun Ji Roh, Kwang-Hun Jeong, Inho Baek, Byoung Ju Kim, Soo-Hong Lee, Inbo Han, Jae Min Cha
Summary: This study successfully applied 3D-spheroids of human bone marrow-dewrived mesenchymal stem cells (MSCs) to a mouse spinal fusion model, achieving more significant improvement compared to conventional 2D-cultured MSCs. The study proposes a GMP-applicable bioprocessing platform and quality control directions for MSC spheroids, aiming for their clinical application in spinal fusion surgery as a new bone graft substitute.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Rheumatology
Shijia Liu, Yingzhuo Wang, Huanhuan Liu, Tingting Xu, Ma-Jie Wang, Jiawei Lu, Yunke Guo, Wenjun Chen, Mengying Ke, Guisheng Zhou, Yan Lu, Peidong Chen, Wei Zhou
Summary: Lipidomics was used to characterize the systemic lipid profile of patients with asymptomatic hyperuricemia (HUA) and gout. Serum biomarkers panel that have the potential to predict and diagnose HUA and gout patients were identified.
Article
Multidisciplinary Sciences
Sirinya Sitthirak, Manida Suksawat, Jutarop Phetcharaburanin, Arporn Wangwiwatsin, Poramate Klanrit, Nisana Namwat, Narong Khuntikeo, Attapol Titapun, Apiwat Jarearnrat, Sakkarn Sangkhamanon, Watcharin Loilome
Summary: This study investigated the association between the intratumoral microbiome and chemotherapeutic resistance in cholangiocarcinoma tissues using 16S rRNA sequencing and 1H NMR spectroscopic analysis. The results showed that specific bacteria and metabolites were significantly different between resistance and sensitive groups, suggesting their potential use as predictive markers for drug responses.
Article
Oncology
Kathryn M. Appleton, Ashley K. Elrod, Katy A. Lassahn, Stephen Shuford, Lillia M. Holmes, Teresa M. DesRochers
Summary: This study examined the effectiveness of using three-dimensional (3D) spheroids to detect patient-specific immune-related activity in ovarian cancer. It found that some patients had higher sensitivity to immune checkpoint inhibitors and demonstrated increases in cytotoxic T cell activation and DC major histocompatibility complex (MHC) class-II expression upon cytokine stimulation. Ex vivo 3D spheroid platforms accurately detected immune cell activity and modulation, providing evidence for their utility in showing sensitivity to ICIs alone or in combination with PARP-Is in a preclinical setting.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Oncology
Bohuslava Vankova, Tomas Vanecek, Nikola Ptakova, Veronika Hajkova, Martin Dusek, Michael Michal, Peter Svajdler, Ondrej Daum, Magdalena Daumova, Michal Michal, Roman Mezencev, Marian Svajdler
GENES CHROMOSOMES & CANCER
(2020)
Article
Pathology
Martina Baneckova, Petr Martinek, Alena Skalova, Roman Mezencev, Ladislav Hadravsky, Michal Michal, Marian Svajdler
Article
Multidisciplinary Sciences
Roman Mezencev, Scott S. Auerbach
Article
Oncology
Roman Mezencev, Yury O. Chernoff
Article
Chemistry, Medicinal
Slavka Hamulakova, Zuzana Kudlickova, Ladislav Janovec, Roman Mezencev, Zachery J. Deckner, Yury O. Chernoff, Jana Janockova, Veronika Ihnatova, Petr Bzonek, Nikola Novakova, Vendula Hepnarova, Martina Hrabinova, Daniel Jun, Jan Korabecny, Ondrej Soukup, Kamil Kuca
Summary: The study reports on the synthesis and biological evaluation of new compounds that combine tacrine and indole moieties. These compounds, particularly 3c, show promising potential as acetylcholinesterase inhibitors and anti-amyloid agents that can cross the blood-brain barrier.
FUTURE MEDICINAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Roman Mezencev, Scott S. Auerbach
Summary: This study analyzed the mechanisms involved in cancer development in mice exposed to Cr(VI) in drinking water, identifying CFTR gene's tumor-suppressor role and supporting the human relevance of Cr(VI)-mediated carcinogenesis in the small intestines of exposed mice. The research also predicted for the first time the role of CFTR inactivation in chemical carcinogenesis and expanded the range of plausible mechanisms involved in Cr(VI)-mediated carcinogenesis of intestinal and possibly other tissues.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Oncology
Peter Svajdler, Peter Vasovcak, Marian Svajdler, Monika Sedivcova, Veronika Urban, Michal Michal, Roman Mezencev
Summary: This study identified a specific germline mutation in the CHEK2 gene that is associated with an increased risk of developing adult-type granulosa cell tumors. Female carriers of this mutation were found to have a significantly higher proportion of ovarian adult-type granulosa cell tumors compared to the general population. These findings suggest potential avenues for the development of targeted interventions for this type of tumor.
Article
Multidisciplinary Sciences
Katherine M. Young, Congmin Xu, Kelly Ahkee, Roman Mezencev, Steven P. Swingle, Tong Yu, Ava Paikeday, Cathy Kim, John F. McDonald, Peng Qiu, Todd Sulchek
Summary: Stiffness decreases with increasing metastatic potential in various cancer cell types, but the molecular factors associated with these phenotypes are unknown. A workflow combining atomic force microscopy and multiplexed RT-qPCR gene expression analysis has been developed to measure mechanical properties and gene expression of single cells. Surprisingly, genes related to extracellular matrix remodeling, epithelial-to-mesenchymal transition, cell adhesion, and cancer stemness showed the strongest correlation with mechanical properties. Cell clustering was improved by combining mechanical and gene expression data types. The single cell genome mechanics method identifies molecular drivers that may affect biophysical processes underlying metastasis.
Review
Medicine, Research & Experimental
Martina Bago Pilatova, Zuzana Solarova, Roman Mezencev, Peter Solar
Summary: Programmed cell death is crucial for maintaining the homeostasis and integrity of multicellular organisms, and dysregulation of this process contributes to various diseases. Ceramides, a group of macromolecules and low-molecular messengers, play important roles in regulating programmed cell death. Different types of ceramides have distinct functions in cell death, and dihydroceramides have emerged as a new class of bioactive sphingolipids with future implications in cancer growth, drug resistance, and tumor metastasis.
ADVANCES IN MEDICAL SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
R. Mezencev, C. Gibbons
Summary: Epidemiological and animal studies have supported the carcinogenicity of hexavalent chromium (Cr(VI)) but the specific molecular changes responsible for this induction of cancer are still not fully understood. The role of oxidative stress and genotoxicity in Cr(VI)-mediated carcinogenesis has been suggested, but the specific types of DNA damage attributed to specific chromium species or other reactive byproducts in biological systems exposed to Cr(VI) have not been definitively determined. The complexity of chromium species and their reactivity with DNA and other biologically relevant molecules in vitro is discussed in this report to provide a more comprehensive understanding of Cr(VI)-mediated toxicity and to reconcile conflicting findings on the biological role of chromium species in previous studies.
Article
Medicine, General & Internal
M. E. Z. E. N. C. E. Roman, K. L. E. M. E. N. T. Cyril
Summary: This study examined the association between the stringency of containment measures and the cumulative incidence of COVID-19 cases in the first wave of the pandemic in 28 European countries. The results support the effectiveness of containment measures in mitigating or suppressing the COVID-19 epidemics. Early adoption of stringent containment measures and increasing containment stringency during the early days of the epidemics were associated with lower disease occurrence. Delayed adoption of stringent measures did not fully compensate for the lack of early response. Containment measures continue to play a significant role in controlling COVID-19 in the post-vaccination period.
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
(2022)
Letter
Immunology
Jana Kerlik, Maria Avdicova, Monika Musilova, Jana Beresova, Roman Mezencev
Summary: This is a case of probable transmission of Tick-borne encephalitis virus (TBEV) from an unvaccinated mother to an infant through breast-feeding. It highlights the importance of being cautious when exposed to infected animals or consuming raw food products, especially for individuals who are not vaccinated.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Immunology
R. Mezencev, C. Klement, S. Dluholucky
Summary: When efficient non-pharmaceutical public health measures are applied to control or mitigate the spread of COVID-19, the catastrophic collision of seasonal influenza and COVID-19 epidemics is unlikely. The extremely low seasonal influenza activity registered in the Southern Hemisphere in 2020 supports this conclusion, although mixed SARS-CoV-2 and influenza virus infections have been demonstrated in humans, highlighting the need for vigilance in the potential severity of these mixed infections.
EPIDEMIOLOGIE MIKROBIOLOGIE IMUNOLOGIE
(2021)
Meeting Abstract
Biophysics
Katherine M. Young, Congmin Xu, Kelly Ahkee, Roman Mezencev, Peng Qiu, Todd Sulchek
BIOPHYSICAL JOURNAL
(2020)
Meeting Abstract
Medicine, Research & Experimental
Marian Svajdler, Michael Michal, Petr Martinek, Zdenek Kinkor, Michal Michal, Peter Szepe, Roman Mezencev, Peter Svajdler
LABORATORY INVESTIGATION
(2019)