Article
Multidisciplinary Sciences
Marietta S. Kaspers, Vivian Pogenberg, Christian Pett, Stefan Ernst, Felix Ecker, Philipp Ochtrop, Michael Groll, Christian Hedberg, Aymelt Itzen
Summary: In this study, the authors uncover the mechanism and substrate recognition of Legionella effector Lem3, which removes a phosphocholine moiety from the human protein Rab1 during infection. They present the crystal structure of the stabilised Lem3:Rab1b complex, revealing the catalytic mechanism and substrate recognition of PPM phosphatases shaped Lem3. This research is of significant importance for understanding bacterial pathogenesis and protein substrate recognition.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Sun Cheol Park, So Yeon Cho, Tae Hee Kim, Kyung Yeol Ko, Wan Seok Song, Seung Goo Kang, Geun-Shik Lee, Sung-il Yoon
Summary: LegK7 is an atypical kinase that activates through interaction with the host MOB1 protein without the need for phosphorylation. Upon binding with MOB1, LegK7 undergoes a structural reshaping that allows it to adopt a closed conformation compatible with ATP binding.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Alix McCloskey, Kayla Perri, TaoTao Chen, Aidong Han, Zhao-Qing Luo
Summary: Legionella pneumophila uses effectors and metaeffectors to manipulate host processes and regulate its virulence through specific mechanisms such as protein modification and direct binding.
MICROBES AND INFECTION
(2021)
Review
Microbiology
Ashley M. Joseph, Stephanie R. Shames
Summary: This review discusses the function of metaeffectors in Legionella pneumophila, which regulate other effectors essential for pathogen replication. While there are at least a dozen known metaeffectors in L. pneumophila, their exact mechanisms of promoting virulence remain largely unknown. The review also highlights challenges in identifying and understanding the contribution of metaeffectors to bacterial pathogenesis.
Article
Multidisciplinary Sciences
Tao-Tao Chen, Yanling Lin, Shijun Zhang, Shuxin Liu, Lei Song, Wenhong Zhong, Zhao-Qing Luo, Aidong Han
Summary: The intracellular bacterial pathogen Legionella pneumophila hijacks host vesicular transport factor p115 to localize its effector protein LegA15 in host lipid droplets (LDs) and causes fragmentation of host Golgi apparatus. LegA15 is an atypical GTPase that modulates host LD homeostasis through its GTPase activity.
Article
Biochemistry & Molecular Biology
Miles H. Black, Adam Osinski, Gina J. Park, Marcin Gradowski, Kelly A. Servage, Krzysztof Pawlowski, Vincent S. Tagliabracci
Summary: ADP-ribosyltransferases (ARTs) are a widespread superfamily of enzymes commonly used by bacteria in pathogenic strategies. Legionella pneumophila has a diverse set of 330 translocated effectors, but the ART effectors influencing this bacteria have not been well defined. The discovery of Legionella ADP-Ribosyltransferase 1 (Lart1) targeting a host metabolic enzyme during infection represents a novel mechanism of pathogenicity for this bacterium.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Bethany Vaughn, Kevin Voth, Christopher T. Price, Snake Jones, Mateja Ozanic, Marina Santic, Miroslaw Cygler, Yousef Abu Kwaik
Summary: The MavE effector of Legionella pneumophila is essential for intracellular growth and lysosomal evasion, playing a crucial role in phagosome biogenesis.
Article
Biochemistry & Molecular Biology
J. Allombert, C. Jaboulay, C. Michard, C. Andrea, X. Charpentier, A. Vianney, P. Doublet
Summary: The Legionella pneumophila Icm/Dot T4SS can translocate a large number of effectors, and research has found that their delivery is controlled by specific timing to effectively manipulate host cells. The timed delivery of effectors depends not only on chaperone protein interactions but also involves cyclic-di-GMP signaling.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Ines P. Monteiro, Sofia Sousa, Vitor Borges, Paulo Goncalves, Joao Paulo Gomes, Luis Jaime Mota, Irina S. Franco
Summary: Two novel effectors, VFX05045 and VFX10045, of Legionella pneumophila were identified and their subcellular localizations were determined. Furthermore, the essential residues for nuclear targeting of VFX10045 were identified.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Tshegofatso Ngwaga, Deepika Chauhan, Stephanie R. Shames
Summary: The article discusses the immune responses mediated by effector proteins in Gram-negative bacterial pathogens and the potential future of developing new drugs using effector proteins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ivy Y. W. Chung, Lei Li, Oleg Tyurin, Alla Gagarinova, Raissa Wibawa, Pengfei Li, Elizabeth L. Hartland, Miroslaw Cygler
Summary: Legionella pneumophila is an intracellular pathogen that causes Legionnaire's disease in humans. It enters host cells through phagocytosis and affects host cellular processes by secreting over 300 effector proteins. RavA, a L. pneumophila effector, displays unique structural characteristics and localizes to the Golgi apparatus and plasma membrane in human cells.
Article
Chemistry, Physical
Tao-Tao Chen, Si-Ru Zheng, Li Tian, Sunliang Lv, Wenhong Zhong, Xiangliang Li, Dandan Zhang, Xuexing Zheng, Songying Ouyang
Summary: Legionella pneumophila, a pathogenic bacterium causing Legionnaires' disease, secretes Lgt family effectors that inhibit protein synthesis in human cells by specific glycosylation of eEF1A. In this study, the structural basis of the action mechanism of Lgt family effectors is revealed. Lgt2 interacts with negatively charged regions in eEF1A, while Lgt1 interacts with a negatively charged surface to inhibit host unfolded protein response. These findings provide insights into the role of glycosyltransferase in Legionella's lifecycle.
Article
Multidisciplinary Sciences
Yunjia Shi, Hongtao Liu, Kelong Ma, Zhao-Qing Luo, Jiazhang Qiu
Summary: Legionella organisms are environmental bacteria that cause Legionnaires' disease. A study characterized an effector protein, RavZ, in L. longbeachae, the second leading cause of Legionella infections. RavZ inhibits autophagy signaling in host cells challenged with L. pneumophila and L. longbeachae, indicating functional redundancy between RavZ(LLO) and RavZ(LP). RavZ(LLO) also reduces cellular levels of polyubiquitinated and polyneddylated proteins. RavZ(LLO) plays a role in the regulation of polyubiquitinated species on the Legionella-containing vacuole during L. longbeachae infection.
Article
Biochemistry & Molecular Biology
Yaobin Liu, Rukmini Mukherjee, Florian Bonn, Thomas Colby, Ivan Matic, Marius Glogger, Mike Heilemann, Ivan Dikic
Summary: The SidE family of Legionella effectors, particularly SdeA, catalyzes non-canonical phosphoribosyl-linked ubiquitination of host proteins to disrupt Golgi integrity. This disruption prevents Golgi linking proteins GRASP55 and GRASP65 from clustering and forming oligomeric structures on multiple serine residues, impacting the host secretory pathway. The Golgi manipulation strategy by Legionella highlights how the bacterium hijacks the secretory pathway to promote bacterial infection.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Cell Biology
Michelle E. Maxson, Yazan M. Abbas, Jing Ze Wu, Jonathan D. Plumb, Sergio Grinstein, John L. Rubinstein
Summary: This study shows that proton-pumping vacuolar H+ ATPases (V-ATPases) are responsible for the acidic environment in secretory and endocytic organelles. The researchers developed a new probe derived from SidK protein to visualize and quantify V-ATPases with high specificity. They found that the density of V-ATPases increases during phagosome maturation and their distribution varies in lysosomes depending on their subcellular localization.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lindsay M. Wright, Emily M. Carpinone, Terry L. Bennett, Mary K. Hondalus, Vincent J. Starai
MOLECULAR MICROBIOLOGY
(2018)
Article
Microbiology
J. L. Baker, E. L. Lindsay, R. C. Faustoferri, T. T. To, E. L. Hendrickson, X. He, W. Shi, J. S. McLean, R. G. Quivey
JOURNAL OF BACTERIOLOGY
(2018)
Article
Multidisciplinary Sciences
Terry L. Bennett, Shannon M. Kraft, Barbara J. Reaves, Joji Mima, Kevin M. O'Brien, Vincent J. Starai
Article
Multidisciplinary Sciences
Emily M. Carpinone, Zhiru Li, Michael K. Mills, Clemence Foltz, Emma R. Brannon, Clotilde K. S. Carlow, Vincent J. Starai
Article
Multidisciplinary Sciences
Anju Sreelatha, Terry L. Bennett, Hui Zheng, Qiu-Xing Jiang, Kim Orth, Vincent J. Starai
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Multidisciplinary Sciences
Anju Sreelatha, Terry L. Bennett, Emily M. Carpinone, Kevin M. O'Brien, Kamyron D. Jordan, Dara L. Burdette, Kim Orth, Vincent J. Starai
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biochemistry & Molecular Biology
Evgeniy Potapenko, Ciro D. Cordeiro, Guozhong Huang, Melissa Storey, Christopher Wittwer, Amit K. Dutta, Henning J. Jessen, Vincent J. Starai, Roberto Docampo
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Cell Biology
Andrew J. Stasic, Nathan M. Chasen, Eric J. Dykes, Stephen A. Vella, Beejan Asady, Vincent J. Starai, Silvia N. J. Moreno
Article
Biochemistry & Molecular Biology
Wei Peng, Amanda K. Casey, Jessie Fernandez, Emily M. Carpinone, Kelly A. Servage, Zhe Chen, Yang Li, Diana R. Tomchick, Vincent J. Starai, Kim Orth
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Microbiology
E. L. Lindsay, R. C. Faustoferri, R. G. Quivey
Summary: Streptococcus mutans, the etiologic agent of dental caries in humans, is a dominating force in the oral microbiome due to its highly-evolved survival propensity. This oral pathogen encodes regulatory elements such as CcpA and TreR, which play key roles in controlling sugar metabolism and stress response. Loss of these regulators impairs growth and fitness response, indicating their involvement in carbon catabolism control and stress response induction.
FEMS MICROBIOLOGY LETTERS
(2021)
Article
Cell Biology
Nathan K. Glueck, Kevin M. O'Brien, Danielle C. Seguin, Vincent J. Starai
Summary: Legionella pneumophila is a facultative intracellular bacterial pathogen that alters host organelle trafficking to construct the bacteria-laden LCV. The effector protein LegC7 functions to recruit host ER material to the bacterial phagosome during infection, potentially through interaction with host membrane tethering complexes and/or cargo adapters, inducing abnormal ER:endosome interactions.
Article
Microbiology
Elizabeth L. Tinder, Roberta C. Faustoferri, Andrew A. Buckley, Robert G. Quivey, Jonathon L. Baker
Summary: This study examines the proteome of Streptococcus mutans during growth in acidic and oxidative stresses, as well as in deletion mutants, and identifies differentially expressed proteins and coexpressed protein modules responsive to stress conditions. The presence of the TreR protein is linked to mutacin production and oxidative stress. This dataset provides valuable insights for understanding S. mutans pathogenesis and developing improved strategies for dental caries prevention and treatment.
Meeting Abstract
Biochemistry & Molecular Biology
Anju Sreelatha, Terry Bennett, Vincent Starai, Kim Orth