Article
Biochemistry & Molecular Biology
Takahide Matsui, Futaba Osaki, Shu Hiragi, Yuriko Sakamaki, Mitsunori Fukuda
Summary: The study demonstrates that different types of small extracellular vesicles, known as exosomes, are differentially secreted from the apical and basolateral sides of polarized epithelial cells. Two independent machineries are responsible for the polarized exosome release from epithelial cells.
Article
Biology
Alberto T. Gatta, Yolanda Olmos, Caroline L. Stoten, Qu Chen, Peter B. Rosenthal, Jeremy G. Carlton
Summary: The ESCRT-III machinery is crucial for sealing the nuclear envelope and repairing it during interphase rupture. The assembly of ESCRT-III at the nuclear envelope is regulated by the interaction between CHMP7 and LEM2, with CDK1 phosphorylation of CHMP7 playing a key role in controlling this process during mitotic exit. Spatial and temporal differences in CHMP7 dephosphorylation also play a role in regulating its assembly at different cellular locations during cell division.
Review
Biochemistry & Molecular Biology
Rostyslav Horbay, Ali Hamraghani, Leonardo Ermini, Sophie Holcik, Shawn T. Beug, Behzad Yeganeh
Summary: In this review, we summarize recent advances on the complex roles of sphingolipids and lysosomes in the process of extracellular vesicle (EVs) biogenesis to uptake.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Chuanliang Liu, Yonglun Zeng, Hongbo Li, Chao Yang, Wenjin Shen, Min Xu, Zhidan Xiao, Tongsheng Chen, Baiying Li, Wenhan Cao, Liwen Jiang, Marisa S. Otegui, Caiji Gao
Summary: The study identified FYVE4 as a novel plant endosomal regulator, functioning in the ESCRT pathway to regulate MVE biogenesis.
Article
Biochemistry & Molecular Biology
Ningjing Liu, Lipan Hou, Xin Chen, Jingjing Bao, Fangyan Chen, Wenjuan Cai, Huixian Zhu, Lingjian Wang, Xiaoya Chen
Summary: In this study, TETRASPANIN8 (TET8) in Arabidopsis thaliana was identified as a carrier protein for sphingolipids and was found to regulate the export of glycosyl inositol phosphoceramides (GIPCs). TET8 interacted with the coat protein complex I (COPI) subunit gamma 2-COPI and facilitated the transportation of GIPCs from the Golgi apparatus to the plasma membrane.
Article
Cell Biology
Jin Dai, Gui Zhang, Rama A. Alkhofash, Betlehem Mekonnen, Sahana Saravanan, Bin Xue, Zhen-Chuan Fan, Ewelina Betleja, Douglas G. Cole, Peiwei Liu, Karl Lechtreck
Summary: The loss of Chlamydomonas ARL13 impedes BBSome-dependent protein transport, resulting in overlapping biochemical defects in arl13 and bbs mutant cilia.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Shuai Hu, Baiying Li, Fan Wu, Dongmei Zhu, Jan Zouhar, Caiji Gao, Tomoo Shimada, Enrique Rojo, Ikuko Hara-Nishimura, Liwen Jiang, Jinbo Shen
Summary: This study elucidates the role and mechanism of ALIX in regulating vacuolar transport of soluble proteins in plants. It is found that ALIX physically interacts with the retromer core subunits Vps26 and Vps29 and cooperatively regulates the trafficking of soluble vacuolar proteins. Depletion of ALIX disrupts the membrane recruitment of Vps26 and Vps29 and alters the endosomal localization of vacuolar sorting receptors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Li Yao, Mei-Yu Ruan, Shi-Wei Ye, Shi-Qing Cai
Summary: Research has discovered the important role of DNA topoisomerase 2-associated proteins, including PATR-1, PATL1, and PATL2, in regulating the biogenesis of the hERG channel. These proteins interact with the transcription factor TFIIE to facilitate the transcription of hERG mRNA. Knockdown of PATL1 and PATL2 leads to decreased protein levels of hERG channels and altered electrophysiological characteristics in the human heart.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Gianluca Figlia, Sandra Mueller, Anna M. Hagenston, Susanne Kleber, Mykola Roiuk, Jan-Philipp Quast, Nora ten Bosch, Damian Carvajal Ibanez, Daniela Mauceri, Ana Martin-Villalba, Aurelio A. Teleman
Summary: The research shows that different Rag GTPase genes and isoforms can differentially regulate mTORC1 activity and modulate the responsiveness of mammalian cells to amino acid availability. This sheds light on the important role of the mechanistic target of rapamycin complex 1 (mTORC1) in regulating cellular anabolism and catabolism.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Peng Yuan, Mengyang Zhang, Lei Tong, Thomas M. Morse, Robert A. McDougal, Hui Ding, Diane Chan, Yifei Cai, Jaime Grutzendler
Summary: In this study, amyloid-plaque-associated axonal spheroids were identified as prominent contributors to neural network dysfunction in Alzheimer's disease. The study found that the accumulation of endolysosomal vesicles and the growth of spheroids were linked with a potential Alzheimer's-disease-associated risk gene, Pld3. Modulation of endolysosomal biogenesis in neurons could potentially reverse axonal spheroid-induced neural circuit abnormalities in Alzheimer's disease, independent of amyloid removal.
Article
Cell Biology
Sebastian Baenfer, Sophie Kutscher, Fenja Fleck, Martina Dienst, Christian Preusser, Elke Pogge von Strandmann, Ralf Jacob
Summary: In this study, we demonstrate that E-cadherin can be recruited into the membrane of extracellular vesicles through a specific domain in its cytoplasmic tail that interacts with Tsg101. This finding contributes to a better understanding of the formation and function of extracellular vesicles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Gabrielle Parkinson, Peristera Roboti, Ling Zhang, Sandra Taylor, Philip Woodman
Summary: HD-PTP collaborates with ESCRTs to sort endosomal cargo into MVB, and is crucial for endosomal maturation. Depletion of HD-PTP inhibits MVB sorting and causes cargo to be trapped in Rab5-rich endosomes.
JOURNAL OF CELL SCIENCE
(2021)
Article
Microbiology
Amanda Bentley-DeSousa, Michael Downey
Summary: Polyphosphates, energy-rich polymers of inorganic phosphates, are present in all cells and involved in various cellular functions. The study focuses on the intracellular trafficking of polyphosphate biosynthetic machinery in yeast and highlights the critical role of the conserved intracellular trafficking complex in regulating polyP levels and defining pathways for polyP regulation in yeast and other species.
Article
Microbiology
Curtis B. Read, Mary Clark H. Lind, Travis J. Chiarelli, Jerilyn R. Izac, Haley E. Adcox, Richard T. Marconi, Jason A. Carlyon
Summary: Anaplasma phagocytophilum exploits multivesicular body biogenesis and trafficking to benefit various stages of its intracellular infection cycle. This bacterium causes granulocytic anaplasmosis, a globally emerging zoonotic disease with limited antibiotic treatment options.
Article
Cell Biology
Kamilla M. E. Laidlaw, Grant Calder, Chris MacDonald
Summary: Laidlaw et al. demonstrate the mechanisms of nutrient transporter recycling, including substrate withdrawal-triggered endosomal recycling pathway and the regulatory role of ubiquitination. These mechanisms play a critical role in controlling the activity of surface membrane proteins.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sophie D. Adams, Judit Csere, Gisela D'angelo, Edward P. Carter, Maryse Romao, Teresa Arnandis, Martin Dodel, Hemant M. Kocher, Richard Grose, Gracca Raposo, Faraz Mardakheh, Susana A. Godinho
Summary: Bidirectional communication between cells and their surrounding environment is crucial, with extracellular vesicles playing a key role in horizontal molecular transfer between cells. Abnormal EV secretion in cancer cells affects tumor growth and metastasis, while centrosome amplification may promote small extracellular vesicle (SEV) secretion, influencing communication between tumor cells and the surrounding stroma.
Article
Multidisciplinary Sciences
Marco Castagna, Sebastien Levilly, Perrine Paul-Gilloteaux, Said Moussaoui, Jean-Marc Rousset, Felicien Bonnefoy, Jerome Idier, Jean-Michel Serfaty, David Le Touze
Summary: This study developed a system based on LDV to validate new methods of 4D Flow MRI acquisition and WSS calculation, utilizing a hydraulic circuit for both MRI and LDV velocity measurements, and calculating WSS profiles using both 2D and 3D methods. Results showed excellent agreement between MRI and LDV velocity data, demonstrating the improved performance of 3D over the 2D-WSS calculation method.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Zackie Aktary, Alejandro Conde-Perez, Florian Rambow, Mathilde Di Marco, Francois Amblard, Ilse Hurbain, Graca Raposo, Cedric Delevoye, Sylvie Coscoy, Lionel Larue
Summary: Dynlt3 plays a crucial role in melanosome transport, maturation, and transfer to keratinocytes, essential for proper skin pigmentation. The Wnt/beta catenin signaling pathway regulates Dynlt3 levels, contributing to the control of melanocyte transport and skin pigmentation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Zoology
Silvia Benito-Martinez, Laura Salavessa, Graca Raposo, Michael S. Marks, Cedric Delevoye
Summary: The synthesis and transfer of melanin in mammalian skin are still debated and poorly understood, but likely play an important role in the photoprotective properties of melanin in the skin.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2021)
Article
Zoology
Linh Le, Julia Sires-Campos, Graca Raposo, Cedric Delevoye, Michael S. Marks
Summary: Melanins, the main pigments of the skin and hair in mammals, are synthesized within membrane-bound organelles of melanocytes called melanosomes. The structure and function of melanosomes are determined by resident transmembrane proteins, defects of which may lead to various forms of albinism. Further investigation is needed to address unanswered questions regarding the molecular mechanisms of melanosome formation and the delivery of specific components.
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2021)
Article
Microscopy
Glyn Nelson, Ulrike Boehm, Steve Bagley, Peter Bajcsy, Johanna Bischof, Claire M. Brown, Aurelien Dauphin, Ian M. Dobbie, John E. Eriksson, Orestis Faklaris, Julia Fernandez-Rodriguez, Alexia Ferrand, Laurent Gelman, Ali Gheisari, Hella Hartmann, Christian Kukat, Alex Laude, Miso Mitkovski, Sebastian Munck, Alison J. North, Tobias M. Rasse, Ute Resch-Genger, Lucas C. Schuetz, Arne Seitz, Caterina Strambio-De-Castillia, Jason R. Swedlow, Ioannis Alexopoulos, Karin Aumayr, Sergiy Avilov, Gert-Jan Bakker, Rodrigo R. Bammann, Andrea Bassi, Hannes Beckert, Sebastian Beer, Yury Belyaev, Jakob Bierwagen, Konstantin A. Birngruber, Manel Bosch, Juergen Breitlow, Lisa A. Cameron, Joe Chalfoun, James J. Chambers, Chieh-Li Chen, Eduardo Conde-Sousa, Alexander D. Corbett, Fabrice P. Cordelieres, Elaine Del Nery, Ralf Dietzel, Frank Eismann, Elnaz Fazeli, Andreas Felscher, Hans Fried, Nathalie Gaudreault, Wah Ing Goh, Thomas Guilbert, Roland Hadleigh, Peter Hemmerich, Gerhard A. Holst, Michelle S. Itano, Claudia B. Jaffe, Helena K. Jambor, Stuart C. Jarvis, Antje Keppler, David Kirchenbuechler, Marcel Kirchner, Norio Kobayashi, Gabriel Krens, Susanne Kunis, Judith Lacoste, Marco Marcello, Gabriel G. Martins, Daniel J. Metcalf, Claire A. Mitchell, Joshua Moore, Tobias Mueller, Michael S. Nelson, Stephen Ogg, Shuichi Onami, Alexandra L. Palmer, Perrine Paul-Gilloteaux, Jaime A. Pimentel, Laure Plantard, Santosh Podder, Elton Rexhepaj, Arnaud Royon, Markku A. Saari, Damien Schapman, Vincent Schoonderwoert, Britta Schroth-Diez, Stanley Schwartz, Michael Shaw, Martin Spitaler, Martin T. Stoeckl, Damir Sudar, Jeremie Teillon, Stefan Terjung, Roland Thuenauer, Christian D. Wilms, Graham D. Wright, Roland Nitschke
Summary: Modern light microscopes have evolved into sophisticated, quantitative devices integral to physical and life science research, but widespread difficulties in evaluating and reproducing scientific experiments highlight a lack of understanding and knowledge in microscope operation and calibration principles. The QUAREP-LiMi initiative aims to establish common quality control standards for light microscopy image data to enhance reproducibility in scientific research, addressing the urgent need for broadly accepted standard practices in the field.
JOURNAL OF MICROSCOPY
(2021)
Editorial Material
Biochemical Research Methods
Ugis Sarkans, Wah Chiu, Lucy Collinson, Michele C. Darrow, Jan Ellenberg, David Grunwald, Jean-Karim Heriche, Andrii Iudin, Gabriel G. Martins, Terry Meehan, Kedar Narayan, Ardan Patwardhan, Matthew Robert Geoffrey Russell, Helen R. Saibil, Caterina Strambio-De-Castillia, Jason R. Swedlow, Christian Tischer, Virginie Uhlmann, Paul Verkade, Mary Barlow, Omer Bayraktar, Ewan Birney, Cesare Catavitello, Christopher Cawthorne, Stephan Wagner-Conrad, Elizabeth Duke, Perrine Paul-Gilloteaux, Emmanuel Gustin, Maria Harkiolaki, Pasi Kankaanpaa, Thomas Lemberger, Jo McEntyre, Josh Moore, Andrew W. Nicholls, Shuichi Onami, Helen Parkinson, Maddy Parsons, Marina Romanchikova, Nicholas Sofroniew, Jim Swoger, Nadine Utz, Lenard M. Voortman, Frances Wong, Peijun Zhang, Gerard J. Kleywegt, Alvis Brazma
Summary: The study proposes draft metadata guidelines to address the needs of diverse communities within light and electron microscopy, with the hope of stimulating discussions about their implementation and future extension.
Article
Biochemistry & Molecular Biology
Julia Sires-Campos, Ana Lambertos, Cedric Delevoye, Graca Raposo, Dorothy C. Bennett, Elena Sviderskaya, Celia Jimenez-Cervantes, Conchi Olivares, Jose Carlos Garcia-Borron
Summary: The absence of MGRN1 leads to cell-autonomous stimulation of pigment production in melanocytes by increasing tyrosinase activity through neutralizing melanosomal pH. Regulating melanosomal pH may involve several key genes such as subunits of V-ATPase and Mucolipin 3.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ilse Hurbain, Anne-Sophie Mace, Maryse Romao, Elodie Prince, Lucie Sengmanivong, Laurent Ruel, Renata Basto, Pascal P. Therond, Graca Raposo, Gisela D'Angelo
Summary: The microvilli in the epithelium of the wing imaginal disc in Drosophila are responsible for the generation of small extracellular vesicles (EVs) that transport the morphogen Hedgehog (Hh) across the tissue. The integrity of microvilli is maintained by the Prominin-like (PromL) protein, which is crucial for its secretory function. Importantly, the distribution of Hh to microvilli and its release via EVs contribute to the proper morphogenesis of the wing imaginal disc.
Article
Multidisciplinary Sciences
Tristan Gomez, Magalie Feyeux, Justine Boulant, Nicolas Normand, Laurent David, Perrine Paul-Gilloteaux, Thomas Freour, Harold Mouchere
Summary: In Vitro Fertilization (IVF) is a common treatment for infertile couples. Time-lapse imaging technology is considered a promising solution to improve embryo quality assessment and the clinical efficiency of IVF. However, the development of AI-based solutions for IVF is limited by the absence of a public reference dataset. This study presents a fully annotated dataset of time-lapse videos of developing embryos, aiming to enhance the application of deep learning in embryo development.
Article
Cardiac & Cardiovascular Systems
Constance Delwarde, Claire Toquet, Pascal Aumond, Amir Hossein Kayvanjoo, Adrien Foucal, Benjamin Le Vely, Manon Baudic, Benjamin Lauzier, Stephanie Blandin, Joelle Veziers, Perrine Paul-Gilloteaux, Simon Lecointe, Estelle Baron, Ilaria Massaiu, Paolo Poggio, Severine Remy, Ignacio Anegon, Herve Le Marec, Laurent Monassier, Jean Jacques Schott, Elvira Mass, Julien Barc, Thierry Le Tourneau, Jean Merot, Romain Capoulade
Summary: This study aimed to identify the main pathophysiological pathways involved in degenerative mitral valve dystrophy (MVD) through multimodality imaging and transcriptomic analysis of a unique knock-in rat model. The study found that extracellular matrix organization, epithelial cell migration, response to mechanical stress, and immune cell contribution are the main signaling pathways leading to MVD.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biology
Tony Durand, Perrine Paul-Gilloteaux, Michalina Gora, Lara Laboudie, Emmanuel Coron, Isabelle Neveu, Michel Neunlist, Philippe Naveilhan
Summary: Full-field optical coherence tomography (FFOCT) allows visualization and analysis of enteric neuronal cells. It can detect changes in the functions of enteric neurons and glia in normal and disease conditions. FFOCT is a suitable method for imaging enteric nervous system activity.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemical Research Methods
Nadav Scher, Katya Rechav, Perrine Paul-Gilloteaux, Ori Avinoam
Summary: This article presents a method for correlating cryo-fluorescence microscopy and cryo-focused ion beam scanning electron microscopy datasets using lipid droplets as in situ fiducials. It provides a step-by-step protocol for sample preparation and imaging, as well as details on performing 3D-correlation with sub-micron accuracy.
Proceedings Paper
Computer Science, Artificial Intelligence
Guillaume Potier, Frederic Lavancier, Stephan Kunne, Perrine Paul-Gilloteaux
Summary: Correlative imaging workflows are widely used in bio-imaging to image the same sample using at least two different and complementary imaging modalities. The estimation of registration error in point-based registration can be solved using multivariate linear regression, providing a framework for estimating the associated error, which can support biologists in analyzing multimodal correlative images.
2021 IEEE INTERNATIONAL CONFERENCE ON IMAGE PROCESSING (ICIP)
(2021)
Article
Biochemistry & Molecular Biology
Graca Raposo, Guillaume van Niel, Philip D. Stahl
Summary: FBA BioAdvances has created a special collections category called FBA special collections (FBA SC) focusing on emerging disciplines such as extracellular vesicles (EVs) and homeostasis. Global leading investigators have contributed articles covering a range of research activities related to EVs biology.