Article
Multidisciplinary Sciences
Zihan Zhao, Xuejiao Xu, Hairong Cheng, Michelle C. Miller, Zhen He, Hongming Gu, Zhongyu Zhang, Avraham Raz, Kevin H. Mayo, Guihua Tai, Yifa Zhou
Summary: Gal-3's N-terminal tail with proline-rich residues plays a crucial role in mediating various cellular activities, affecting cell migration, activation, endocytosis, and hemagglutination. The research also reveals the significant impact of prolines in regulating Gal-3 oligomerization process.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Chong Ou, Chao Li, Chiguang Feng, Xin Tong, Gerardo R. Vasta, Lai-Xi Wang
Summary: A facile synthesis of beta-cyclodextrin-based multivalent ligands containing Tn and TF antigens was reported. These synthetic multivalent glycan ligands demonstrated enhanced binding affinity and clustering effect in binding to human Gal-3. The GalNAc-containing heptavalent ligand showed the highest affinity and the synthetic ligands could efficiently inhibit Gal-3 binding to human airway epithelial cells.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Alex D. Ho, Shang-Chuen Wu, Nourine A. Kamili, Anna V. Blenda, Richard D. Cummings, Sean R. Stowell, Connie M. Arthur
Summary: Numerous studies have shown the utility of glycan microarray analysis in understanding protein-glycan interactions. However, current studies often analyze these interactions at a single protein concentration, which fails to account for printing variations or other factors that may affect binding. To overcome this limitation, a study examined glycan array binding of three galectins over a range of concentrations and developed a method to optimize the efficiency of assessing association constants. The results demonstrate an effective approach to glycan array analysis that provides improved detail and efficiency compared to previous methods.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Multidisciplinary Sciences
Weizhen Jia, Lingyu Kong, Hiroyasu Kidoya, Hisamichi Naito, Fumitaka Muramatsu, Yumiko Hayashi, Han-Yun Hsieh, Daishi Yamakawa, Daniel K. Hsu, Fu-Tong Liu, Nobuyuki Takakura
Summary: This study demonstrates that Gal-3 expression in long-term repopulating HSCs is induced in response to Tie2 and Mpl, and plays a crucial role in maintaining HSC quiescence through regulation of p21.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Sarah-Jane Richards, Tessa Keenan, Jean-Baptiste Vendeville, David E. Wheatley, Harriet Chidwick, Darshita Budhadev, Claire E. Council, Claire S. Webster, Helene Ledru, Alexander N. Baker, Marc Walker, M. Carmen Galan, Bruno Linclau, Martin A. Fascione, Matthew I. Gibson
Summary: By integrating non-natural fluorinated glycans into nanomaterials, unprecedented selectivity can be achieved in biosensors with potential applications.
Article
Multidisciplinary Sciences
Shaheen A. Farhadi, Renjie Liu, Matthew W. Becker, Edward A. Phelps, Gregory A. Hudalla
Summary: Galectin-3 exhibits dynamic oligomerization and promiscuous binding, affecting cell behavior by influencing signaling pathways. Synthetic Gal3 constructs with defined valency can physically regulate extracellular signaling activity and cell death induction. Changes in multivalency impact caspase activation, membrane glycoprotein engagement, and intracellular pathway activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Gastroenterology & Hepatology
Diyoly Ayona, Sandra Madariaga Zarza, Ludovic Landemarre, Benoit Roubinet, Philippe Decloquement, Didier Raoult, Pierre-Edouard Fournier, Benoit Desnues
Summary: Tropheryma whipplei is an actinobacterium that causes various infections in humans, including Whipple's disease. The bacterium's complex surface glycoproteins contain different sugars, and they interact with Galectin-1 and Galectin-3 to promote infection.
Article
Cell Biology
Lingxin Zhu, Yi Tang, Xiao-Yan Li, Samuel A. Kerk, Costas A. Lyssiotis, Xiaoyue Sun, Zijun Wang, Jung-Sun Cho, Jun Ma, Stephen J. Weiss
Summary: The interaction between metalloproteins Mmp9 and Mmp14 and carbohydrate-binding protein galectin-3 plays a critical role in osteoclast-mediated bone resorption. The ability of Mmp9 and Mmp14 to degrade galectin-3 on the cell surface depends on its binding protein Lrp-1. These findings reveal a previously unrecognized galectin-3/Lrp1 axis that regulates both the transcriptional programs and intracellular signaling cascades essential for osteoclast function.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Shihan Zhen, Ruoxin Cai, Xuelian Yang, Yanan Ma, Deliang Wen
Summary: The study revealed a positive association between serum GAL-3BP levels and MetS, especially in postmenopausal women. Additionally, increased expression of GAL-3BP in the serum and visceral adipose tissue of high fat diet mice suggests its relevance to MetS.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Fei Fei, Mingfeng Zhang, Somayeh S. Tarighat, Eun Ji Joo, Lu Yang, Nora Heisterkamp
Summary: Galectin-1 and Galectin-3 have a complicated joint contribution to BCP-ALL survival, with stromal Galectin-3 playing an important role in maintaining BCP-ALL fitness during chemotherapy exposure, while stromal Galectin-1 does not significantly contribute to drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Adel Abo Mansour, Federica Raucci, Mustafa Sevim, Anella Saviano, Jenefa Begum, Zhaogong Zhi, Laleh Pezhman, Samantha Tull, Francesco Maione, Asif Jilani Iqbal
Summary: This study investigates the role of Gal-9 in the migration and adhesion of CD4+ and CD8+ T cells and reveals its potential therapeutic significance in inflammatory-based diseases. The findings suggest that Gal-9 supports T cell adhesion and transmigration in a glycan dependent manner and inhibits the chemotactic function of CXCL12 through direct binding.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Endocrinology & Metabolism
Zeqing Zhang, Xuehui Kang, Yaming Guo, Jianhua Zhang, Junhui Xie, Shiying Shao, Ying Xiang, Gang Chen, Xuefeng Yu
Summary: The study found that circulating galectin-3 levels may be associated with GDM, potentially mediated through increased insulin resistance. Additionally, there is an association between galectin-3 and progesterone, highlighting a potential role of progesterone in its interaction with galectin-3.
JOURNAL OF DIABETES
(2021)
Article
Medicine, General & Internal
Po-Yu Huang, Chen-Sen Huang, Yu-Li Lin, Yi-Hsin Chen, Szu-Chun Hung, Jen-Pi Tsai, Bang-Gee Hsu
Summary: A novel cardiovascular stress biomarker called galectin-3 shows potential for predicting adverse cardiovascular outcomes. This study aimed to assess the association between serum galectin-3 levels and aortic stiffness in peritoneal dialysis patients. The results demonstrated that serum galectin-3 levels were independently associated with carotid-femoral pulse wave velocity and aortic stiffness in peritoneal dialysis patients, along with gender and age.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Zak Vilen, Eugene Joeh, Meg Critcher, Christopher G. Parker, Mia L. Huang
Summary: Galectin-1 is a key protein in promoting myogenic differentiation, but the glycoprotein interactors of galectin-1 are not fully understood. A proximity tagging strategy has been used to capture and identify the glycan-mediated glycoprotein interactors of galectin-1 in myoblasts, providing valuable resources for studying the mechanisms through which galectin-1 promotes myogenic differentiation.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Stellee M. P. Biscaia, Cassiano Pires, Francislaine A. R. Livero, Daniel L. Bellan, Israel Bini, Silvina O. Bustos, Renata O. Vasconcelos, Alexandra Acco, Marcello Iacomini, Elaine R. Carbonero, Martin K. Amstalden, Fabio R. Kubata, Richard D. Cummings, Marcelo Dias-Baruffi, Fernanda F. Simas, Carolina C. Oliveira, Rilton A. Freitas, Celia Regina Cavichiolo Franco, Roger Chammas, Edvaldo S. Trindade
Summary: Melanoma is a highly metastatic and rapidly progressing cancer. The melanoma microenvironment plays a crucial role in drug resistance and tumor recurrence. The study found that MG-Pe can bind to Galectin-3 and has antitumor and antimetastatic effects, especially when used in combination with chemotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)