Article
Biochemistry & Molecular Biology
Jie-Ning Li, Pai-Sheng Chen, Ching-Feng Chiu, Yu-Jhen Lyu, Chiao Lo, Li-Wei Tsai, Ming-Yang Wang
Summary: This study reveals the regulatory role of TARBP2 in the ubiquitination-proteasomal degradation of HIF-1 alpha protein in breast cancer, showing its significance in cancer progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Roman Marti-Diaz, Maria F. Montenegro, Juan Cabezas-Herrera, Colin R. Goding, Jose Neptuno Rodriguez-Lopez, Luis Sanchez-del-Campo
Summary: The study found that HIF-1α is constitutively expressed in melanoma cells under normoxic conditions and its elevated expression is associated with the aggressiveness of melanoma tumors. Using acriflavine as an inhibitor of HIF-1α dimerization, the researchers investigated whether HIF-1α-regulated pathways contribute to the growth of melanoma cells under normoxia. Results showed that acriflavine can differentially modulate HIF-1α-regulated targets, inhibiting glucose metabolism and the Warburg effect, ultimately leading to cell death.
Article
Biochemistry & Molecular Biology
Kshitiz, Junaid Afzal, Yasir Suhail, Hao Chang, Maimon E. Hubbi, Archer Hamidzadeh, Ruchi Goyal, Yamin Liu, Peng Sun, Stefania Nicoli, Chi Dang, Andre Levchenko
Summary: The response of individual cancer cells to hypoxic conditions is dynamic and variable, with oscillatory activity being one of the response classes. Reactive-oxygen-species-dependent chaperone-mediated autophagy of HIF-1 alpha plays a role in regulating these responses in some cells. The abundance of extracellular lactate modulates the oscillatory response.
Article
Cell Biology
Kateryna Kubaichuk, Thomas Kietzmann
Summary: Colorectal cancer is a common malignant disease and a leading cause of cancer-related deaths worldwide. In this study, we identified the deubiquitinase USP10 as a crucial player in colon cancer progression and hypoxia response modulation. Knockout of USP10 led to increased HIF-1α protein levels, which affected cellular migration, adhesion, and energy metabolism in colon cancer cells. The USP10-dependent increase in HIF-1α was mediated by mTOR/S6K signaling and not transcription or protein stability. These findings suggest that USP10 could be a potential therapeutic target for colon cancer.
Article
Multidisciplinary Sciences
Tatsuya Sato, Nobutoshi Ichise, Takeshi Kobayashi, Hiroyori Fusagawa, Hiroya Yamazaki, Taiki Kudo, Noritsugu Tohse
Summary: The initiation of heartbeat in the heart primordium leads to changes in metabolic characteristics, including increased ATP levels and decreased reduced glutathione levels. Glycolysis and ATP synthesis-linked mitochondrial respiration are enhanced, but subunits in complexes of mitochondrial oxidative phosphorylation are not upregulated. HIF-1 alpha is activated and related enzymes in metabolic pathways are upregulated to meet the increased energy demand in the heart primordium.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Yu Zhuang, Zhiyang Zhao, Mengjia Cheng, Meng Li, Jiawen Si, Kaili Lin, Hongbo Yu
Summary: Periosteum plays a crucial role in bone repair and contains skeletal stem cells (SSCs) that contribute to endogenous bone regeneration. This study investigated the effect of HIF-1 alpha on periosteum-derived stem cells (PDSCs) and the mechanisms of PDSCs activation in a hypoxic environment. The results showed that removing periosteum severely impaired bone regeneration and pluripotent PDSCs isolated from periosteum expressed mesenchymal stem cell (MSC) markers. Knockdown of HIF-1 alpha expression impaired bone regeneration and osteogenesis of PDSCs, which could be partly rescued by recombinant POSTN. Furthermore, the PI3K/AKT/POSTN pathway was found to be involved in the regulation of PDSCs osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hyun-Yoo Joo, Jin Kyu Jung, Mi-Yeon Kim, Seon Rang Woo, Jae Min Jeong, Eun-Ran Park, Yong-Min Kim, Joong-Jean Park, Joon Kim, Miyong Yun, Hyun-Jin Shin, Kee-Ho Lee
Summary: This study reveals that the high concentration of pyruvate in the culture medium can affect the stability of HIF-1α, and NADH serves as a signal for the degradation of HIF-1α under chronic hypoxia through the SIRT1 and VHL signaling pathway. This degradation leads to a reduction in hypoxia-enhanced invasion and angiogenic activities. Additionally, the study demonstrates that VHL is involved in the degradation of HIF-1α through recognition of hydroxylation in normoxia and acetylation in hypoxia.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Liang Chen, Jin Yang, Meng Zhang, Donglin Fu, Huan Luo, Xiaolei Yang
Summary: ARDS is a severe inflammatory pulmonary condition associated with the imbalance of Th17/Treg and M1/M2. Understanding the regulation of this balance may lead to novel therapeutic targets for ARDS.
Article
Veterinary Sciences
Xiaohua Du, James Blackar Mawolo, Xia Liu, Xiaoyu Mi, Qiao Li, Yongqiang Wen
Summary: The study explored the expression of Ngb and Hif-1 alpha in the telencephalon of adult yak, revealing their significance in neuroprotection and adaptation to high-altitude environments. Both proteins participate in oxygen transport throughout the telencephalon, with Ngb showing higher expression compared to Hif-1 alpha, especially in regions such as the cerebellar cortex, hippocampus, amygdala, cerebellum, and corpus callosum. Further research is needed to confirm the adaptive mechanisms at play.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Environmental Sciences
Ze Zhang, Liu Wu, Tenglong Cui, Rifat Zubair Ahmed, Haiyi Yu, Rong Zhang, Yanhong Wei, Daochuan Li, Yuxin Zheng, Wen Chen, Xiaoting Jin
Summary: Exposure to ambient PM2.5 leads to the upregulation of HIF-1α protein in mouse myocardium, resulting in myocardial injury and hypertrophy. PM2.5-induced HIF-1α accumulation is responsible for the promotion of myocardial hypoxia injury.
ENVIRONMENTAL POLLUTION
(2022)
Article
Oncology
Linling Zhang, Jingjing Ke, Shengping Min, Nan Wu, Fei Liu, Zhen Qu, Wei Li, Hongtao Wang, Zhongqing Qian, Xiaojing Wang
Summary: The study showed that HBO suppresses hypoxia-induced HIF-1 alpha expression and downstream signaling in NSCLC cells, leading to inhibition of the Warburg effect, hyperproliferation, and epithelial-to-mesenchymal transition. The HIF-1 alpha target gene PFKP plays a central role in mediating HBO's effects and may be a potential metabolic vulnerability in NSCLC tumors.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ziqi Yang, Biyan Ni, Tian Zhou, Zijing Huang, Hong Zhou, Yang Zhou, Shiya Lin, Chang He, Xialin Liu
Summary: This study discovered an unexpected anti-angiogenic effect of large diameter intravitreal punctures, which significantly suppressed retinal neovascularization, improved oxygenation, and reduced inflammatory responses. Lowering intraocular pressure may be a promising therapeutic avenue for treating retinal neovascular diseases.
Article
Green & Sustainable Science & Technology
Ali Sheikhbabaei, Aida Hosseini Baghanam, Mahdi Zarghami, Sepideh Pouri, Elmira Hassanzadeh
Summary: This paper presents a streamflow prediction model for the Aji-Chay watershed in Iran, using ensemble multi-GCM downscaling and system dynamics. It assesses future precipitation and temperature using statistical downscaling, develops a rainfall-runoff model using artificial neural networks, and creates an SD model for sustainable development. The results indicate changes in temperature and precipitation and reveal the importance of implementing multiple strategies for sustainable water management.
Article
Neurosciences
Yuanyuan Zhang, Pengyu Jia, Kui Wang, Yan Zhang, Yuying Lv, Pei Fan, Liufei Yang, Shuyue Zhang, Tianyue Wang, Jing Zhao, Haixia Lv, Xinlin Chen, Yong Liu, Haidong Wei, Pengbo Zhang
Summary: Metabolic adaption drives microglial inflammatory responses, and lactate shapes immunological and inflammatory states. In this study, the researchers investigated the involvement of lactate in regulating microglial inflammatory responses after cerebral ischemia. They found that lactate treatment inhibited the increase in inflammatory markers and NF-kappa B pathway induced by oxygen-glucose deprivation in BV2 cells. Lactate also activated HIF-1 alpha, which played a role in the protective response of microglia. Furthermore, lactate-treated BV2 cells improved the viability and reduced the apoptosis of neurons after ischemia. These findings suggest that lactate can modulate microglial inflammatory responses and promote neuronal survival.
BRAIN RESEARCH BULLETIN
(2023)
Article
Biochemistry & Molecular Biology
Joao Seco, Clarence C. King, Gianmarco Camazzola, Jeannette Jansen, Luca Tirinato, Maria G. Marafioti, Rachel Hanley, Francesca Pagliari, Scott P. Beckman
Summary: This article proposes a novel mechanism that cancer cells can modulate the oxygen concentration within the nucleus, allowing them to resist chemo- and radio-therapy. The mechanism depends on the cholesterol content of cellular membranes and the competition between oxygen diffusion and oxygen consumption within the cell. Experimental evaluation of cellular and nucleus oxygen concentration during the cell cycle confirms the hypothesis and indicates increased radiation resistance during the S-phase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
History & Philosophy Of Science
Baptiste Bedessem
Summary: The issue of conservatism in scientific research focuses on the internal and external forces influencing the emergence of new research questions and the use of risky methods. Current literature explores the factors that promote conservative tendencies in scientific research. This paper contributes to the debate by clarifying internal sources of conservatism inherent to the research process, including difficulties in setting aside established conceptual frameworks and the practical conservatism inherent in scientific activities.
Article
Biology
Pierre Jacquet, Angelique Stephanou
Summary: Using the right terminology is crucial in accurately describing biological phenomena, as inappropriate use may lead to biases in understanding, which can have negative consequences. This article focuses on the term "reprogramming" associated with cancer cell metabolism, highlighting inconsistencies in its usage and criticizing the overemphasis on the role of mutations in metabolic adaptation. With the growing interest in metabolism and the Warburg effect in cancer research, it is important to discuss these terms and concepts in detail.
Article
Area Studies
Baptiste Bedessem, Bogna Gawronska-Novak, Piotr Lis
Summary: The study assesses the relationship between citizens' participation in scientific research and public trust in research results within social sciences. Through an online citizen science quasi-experiment conducted in two stages, it is found that the participation of local residents in the research process can increase public trust in the study results.
JOURNAL OF CONTEMPORARY EUROPEAN RESEARCH
(2021)
Editorial Material
Biology
A. Stephanou
PHYSICS OF LIFE REVIEWS
(2022)
Article
Multidisciplinary Sciences
Baptiste Bedessem, Romain Julliard, Eleonora Montuschi
Summary: This study provides a comparative evaluation of the scientific impact of a citizen science program in ecology managed by the French National Museum of Natural History. The results suggest that the yearly number of citations of articles from the program is significantly higher than other publications in the same domain, and this excess of citations tends to steadily grow over time. This indicates that large-scale biodiversity citizen science projects can have a high epistemic impact, but further investigations are needed to clarify specific management methods.
Article
Ethics
Ana-Cristina Torres, Baptiste Bedessem, Nicolas Deguines, Colin Fontaine
Summary: By studying an online communication space, we found that participants in citizen science projects share data and play various roles in this space, such as helping identify species, monitoring scientific research, and maintaining community life, which promotes exchanges and reflections on biodiversity. The existence of this space helps produce high-quality data, science learning, individual commitment to environmental issues, and enables citizens to build and strengthen collective epistemic and affective relationships with science.
JOURNAL OF RESPONSIBLE INNOVATION
(2023)
Article
Green & Sustainable Science & Technology
Baptiste Bedessem, Lucie Morere, Louise Roblin, Anne Dozieres, Anne-Caroline Prevot
Summary: This paper aims to identify obstacles and tools for public participation in biodiversity governance through a comparative analysis of two case studies. The results suggest that skepticism exists regarding the political outputs of public participation, especially when the objectives and means are not clear enough. Furthermore, the efficiency of participative biodiversity governance depends on institutions proposing a variety of citizen engagement formats and objectives to reflect diverse motivations and preferences.
Review
Biochemistry & Molecular Biology
Pierre Jacquet, Angelique Stephanou
Summary: This review explores the evolving understanding of tumor cell metabolism, particularly glycolytic and oxidative metabolism, tracing its evolution from the pioneering work of Otto Warburg. Misinterpretations of Warburg's work have hindered the understanding of tumor cell metabolism, leading to new concepts like metabolic switch and metabolic reprogramming. The metabolic signature of cancer is complex and cannot be simplified to a single factor, but is expressed at the tissue level through cells' ability to dynamically explore a vast metabolic landscape in heterogeneous environments.
Article
Biology
Pierre Jacquet, Angelique Stephanou
Summary: Cancer cells' metabolism is of great interest in cancer research. However, there are few theoretical models addressing this issue due to the complexity of the metabolism. This study proposes a simplified model focusing on the interrelation of three main energy metabolites to better understand the dynamics of the core metabolic system. The model highlights the main rules allowing cells to adapt their metabolism to changing environments and suggests that metabolic features attributed to cancer cells may be due to the deregulated over-acidic environment rather than an intrinsic abnormality of the cells.
JOURNAL OF THEORETICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alaa Tafech, Celine Beaujean, Yves Usson, Angelique Stephanou
Summary: There are two groups of fluorescent probes for pH measurements in biological systems. The first group exhibits a single fluorescence emission peak, while the second group exhibits a dual fluorescence emission peak. This study presents a methodology using multiple wavelengths excitation to evaluate the precision of ratiometric measurements for pH determination in biological samples.
Article
Biology
Alaa Tafech, Pierre Jacquet, Celine Beaujean, Arnold Fertin, Yves Usson, Angelique Stephanou
Summary: Tumor cells have high metabolic activity, leading to increased acidity in the surrounding environment. This acidic environment promotes tumor aggressiveness and invasiveness, which are associated with a worse prognosis. This study found that different types of brain tumor cells handle acidity differently. The ability to regulate acidity appears to depend on the specific cell type and involves various mechanisms. These findings could impact treatment, as some drugs work better in certain pH environments. Adjusting the extracellular pH may improve drug effectiveness.
Review
Multidisciplinary Sciences
Dilek Fraisl, Gerid Hager, Baptiste Bedessem, Margaret Gold, Pen-Yuan Hsing, Finn Danielsen, Colleen B. Hitchcock, Joseph M. Hulbert, Jaume Piera, Helen Spiers, Martin Thiel, Mordechai Haklay
Summary: This article introduces citizen science as an effective method in the environmental and ecological sciences, highlighting its full life cycle and addressing the challenges it faces. The article also provides examples to showcase the diverse applications of citizen science in various domains.
NATURE REVIEWS METHODS PRIMERS
(2022)
Article
Computer Science, Interdisciplinary Applications
I. Manifacier, A. Chauviere, C. Verdier, G. Chagnon, I. Cheddadi, N. Glade, A. Stephanou
COMPUTER METHODS IN BIOMECHANICS AND BIOMEDICAL ENGINEERING
(2020)
Review
Information Science & Library Science
Baptiste Bedessem
RESEARCH EVALUATION
(2020)
Article
Mathematical & Computational Biology
Angelique Stephanou, Pascal Ballet, Gibin Powathil
MATHEMATICAL MODELLING OF NATURAL PHENOMENA
(2020)