Article
Chemistry, Multidisciplinary
Zhiwei Ye, Wei Yang, Ying Zheng, Shujing Wang, Xiaodong Zhang, Haibo Yu, Shuangshuang Li, Chunxiong Luo, Xiaojun Peng, Yi Xiao
Summary: In this study, a new membrane probe and microfluidic platform were developed to enable synchronous use of super-resolution imaging and single-molecule tracking. This method allows high-throughput observation and analysis of the ultrastructural and dynamic details of living red blood cell membranes, providing new perspectives for future disease diagnostics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biotechnology & Applied Microbiology
Weisong Zhao, Shiqun Zhao, Liuju Li, Xiaoshuai Huang, Shijia Xing, Yulin Zhang, Guohua Qiu, Zhenqian Han, Yingxu Shang, De-En Sun, Chunyan Shan, Runlong Wu, Lusheng Gu, Shuwen Zhang, Riwang Chen, Jian Xiao, Yanquan Mo, Jianyong Wang, Wei Ji, Xing Chen, Baoquan Ding, Yanmei Liu, Heng Mao, Bao-Liang Song, Jiubin Tan, Jian Liu, Haoyu Li, Liangyi Chen
Summary: Sparse structured illumination microscopy (Sparse-SIM) achieves nearly twofold resolution enhancement by utilizing sparse deconvolution algorithm, enabling the resolution of intricate biological structures such as small fusion pores, nuclear pores, and relative movements of inner and outer mitochondrial membranes.
NATURE BIOTECHNOLOGY
(2022)
Article
Optics
Zewei Luo, Ge Wu, Mengting Kong, Zhi Chen, Zhengfei Zhuang, Junchao Fan, Tongsheng Chen
Summary: Forster resonance energy transfer (FRET) microscopy is a valuable tool for studying biological systems, but super-resolution FRET imaging in living cells is challenging. In this study, we propose a SR FRET method called SIM-FRET that combines SR structured illumination microscopy (SIM) imaging and acceptor sensitized emission FRET imaging to achieve live-cell quantitative SR FRET imaging. Our method provides enhanced spatial resolution and maintains the advantages of quantitative FRET analysis. We validate the effectiveness of SIM-FRET through simulated models and live-cell samples, and demonstrate its ability to reveal the intricate structure of FRET signals.
PHOTONICS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Rajesh Ranjan, Xin Chen
Summary: This study presents a method for conducting super-resolution timelapse fluorescence live cell imaging in situ, compatible with low fluorescent intensity cells and capable of visualizing multiple subcellular structures. Special procedures in sample preparation and immobilizing specimens are necessary for this technique, but it is broadly applicable to various cell types.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Chemistry, Multidisciplinary
Raveen K. G. Tank, Victoria A. Lund, Sandip Kumar, Robert D. Turner, Lucia Lafage, Laia Pasquina Lemonche, Per A. Bullough, Ashley Cadby, Simon J. Foster, Jamie K. Hobbs
Summary: This study combines STORMForce and SIMForce technologies to map the synthesis of peptidoglycan, a structural macromolecule of bacterial cell walls, during growth and division in Bacillus subtilis. It reveals a two-stage process of septal synthesis during division and bands of synthesis during growth. The combination of super-resolution optics and AFM provides insights into the synthesis processes of bacterial structural biopolymers.
Article
Biochemical Research Methods
Romain F. Laine, Hannah S. Heil, Simao Coelho, Jonathon Nixon-Abell, Angelique Jimenez, Theresa Wiesner, Damian Martinez, Tommaso Galgani, Louise Regnier, Aki Stubb, Gautier Follain, Samantha Webster, Jesse Goyette, Aurelien Dauphin, Audrey Salles, Sian Culley, Guillaume Jacquemet, Bassam Hajj, Christophe Leterrier, Ricardo Henriques
Summary: This article presents an enhanced super-resolution radial fluctuations (eSRRF) method that improves image fidelity and resolution. The method incorporates automated parameter optimization and has been validated across various imaging modalities and biological systems. Additionally, eSRRF has been extended to three dimensions by combining it with multifocus microscopy, enabling live-cell volumetric super-resolution imaging.
Article
Chemistry, Multidisciplinary
Hao Luo, Chaodi Jiang, Yangdong Wen, Xiaoduo Wang, Feifei Wang, Lianqing Liu, Haibo Yu
Summary: The resolution of fluorescence imaging has been enhanced by the development of super-resolution techniques, but the resolution of bright-field imaging lags behind. This study uses microsphere superlenses to simultaneously enhance the resolution of both imaging techniques and achieves correlated super-resolution imaging. This allows for clear observation of the precise distribution of nanoparticles in cells and has the potential to capture ultrafast biological activity with high temporal resolutions.
Article
Multidisciplinary Sciences
Vytautas Navikas, Samuel M. Leitao, Kristin S. Grussmayer, Adrien Descloux, Barney Drake, Klaus Yserentant, Philipp Werther, Dirk-Peter Herten, Richard Wombacher, Aleksandra Radenovic, Georg E. Fantner
Summary: The combination of scanning ion conductance microscopy (SICM) and live-cell super-resolution optical fluctuation imaging (SOFI) is introduced for non-invasive topographical imaging of soft biological samples, preserving high axial resolution. This method allows for the visualization of cellular structures in 3D with high resolution while maintaining the integrity of fragile membrane samples.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Jacob A. Erstling, Joshua A. Hinckley, Nirmalya Bag, Jessica Hersh, Grant B. Feuer, Rachel Lee, Henry F. Malarkey, Fei Yu, Kai Ma, Barbara A. Baird, Ulrich B. Wiesner
Summary: The use of ultrasmall fluorescent core-shell aluminosilicate nanoparticles (aC' dots) enables brighter and more photostable STORM imaging, allowing for quantitative assessment of cellular structures and processes.
ADVANCED MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Karolina Szafranska, Tanja Neuman, Zbigniew Baster, Zenon Rajfur, Oskar Szelest, Christopher Holte, Agata Kubisiak, Edyta Kus, Deanna L. Wolfson, Stefan Chlopicki, Balpreet S. Ahluwalia, Malgorzata Lekka, Marek Szymonski, Peter McCourt, Bartlomiej Zapotoczny
Summary: In this study, different types of microscopy techniques were compared to analyze fenestrations in liver sinusoidal endothelial cells (LSEC). Sample dehydration was found to alter fenestration diameters, and a combination of atomic force microscopy (AFM) with conventional microscopy was proposed to observe cell dynamics and obtain chemical information more effectively.
Article
Physics, Multidisciplinary
Yun-Qing Tang, Cai-Wei Zhou, Hui-Wen Hao, Yu-Jie Sun
Summary: A fully convolutional encoder-decoder network (FCEDN) was developed and applied to image scanning microscopy (ISM), achieving super-resolution imaging. The study demonstrated the feasibility and effectiveness of using this network in different microscopy systems.
Article
Neurosciences
Stefano Calovi, Federico N. Soria, Jan Tonnesen
Summary: STED microscopy is a super-resolution technique that allows imaging at higher spatial resolution than the diffraction limit of light, particularly well-suited for imaging inside live brain tissue. Over the past 20 years, STED microscopy has undergone extensive developments and facilitated remarkable neurophysiological discoveries.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Dokyung Jeong, Doory Kim
Summary: The combination of correlative super-resolution fluorescence microscopy (SRM) and electron microscopy (EM) is an effective hybrid technique that allows the simultaneous acquisition of spatial locations of specific molecules and the context of cellular ultrastructure. Although there are challenges in integrating these two microscopy techniques, extensive research has led to significant improvements and widespread applications.
MOLECULES AND CELLS
(2022)
Article
Chemistry, Multidisciplinary
Michael Holtmannspoetter, Eike Wienbeuker, Timo Dellmann, Isabelle Watrinet, Ana J. Garcia-Saez, Kai Johnsson, Rainer Kurre, Jacob Piehler
Summary: Self-labeling enzymes (SLE) like the HaloTag are valuable tools in high and super-resolution fluorescence microscopy. New fluorogenic substrates for SLE allow imaging with excess dye. Two engineered variants of HaloTag7 with restored dehalogenase activity, reHaloTagS (approx. 0.006 s(-1)) and reHaloTagF (approx. 0.055 s(-1)), showed different turnover kinetics in vitro. Imaging with reHaloTag labeling demonstrated 3-5 times enhanced photostability, and single molecule imaging enabled controlled and stable labeling density over extended periods. Combination with structured illumination allowed simultaneous visualization of single molecule diffusion and organellar dynamics. These applications showcase the potential of reHaloTag labeling in advancing fluorescence microscopy techniques.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Multidisciplinary
Adelaide Miranda, Ana I. Gomez-Varela, Andreas Stylianou, Liisa M. Hirvonen, Humberto Sanchez, Pieter A. A. De Beule
Summary: The invention of the Atomic Force Microscope (AFM) in 1986 allowed for the study of cellular specimens under nearly physiological conditions with nanometric resolution, and the application of AFM to biological research expanded with improvements in imaging modes. Advances in super-resolution fluorescence microscopy techniques have brought the resolution of light microscopy to a level comparable to that of AFM, leading to the combination of AFM with fluorescence based super-resolution microscopy modalities.
Article
Biochemical Research Methods
Andrew J. Bower, Tao Liu, Nancy Aguilera, Joanne Li, Jianfei Liu, Rongwen Lu, John P. Giannini, Laryssa A. Huryn, Alfredo Dubra, Zhuolin Liu, Daniel X. Hammer, Johnny Tam
Summary: The study developed an imaging platform combining multimodal AO-SLO with AO-OCT to compare four different modalities for imaging RPE cells in the same eye. Consistent cellular structure information was obtained across modalities, with variations in RPE cell contrast explored in a subject-, location-, and even cell-dependent manner. The benefit of using multiple modalities to infer the cellular structure of the RPE mosaic in an affected eye was illustrated through multimodal imaging in a patient with choroideremia.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Geriatrics & Gerontology
Joanne Li, Tao Liu, Oliver J. Flynn, Amy Turriff, Zhuolin Liu, Ehsan Ullah, Jianfei Liu, Alfredo Dubra, Mary A. Johnson, Brian P. Brooks, Robert B. Hufnagel, Daniel X. Hammer, Laryssa A. Huryn, Brett G. Jeffrey, Johnny Tam
Summary: A 3-year longitudinal study on a patient with oligocone trichromacy (OT) revealed persistent dark cones within and near the macula, but the patient had normal visual acuity, retinal sensitivity, and color vision. The findings suggest that a retina with predominant dark cones can support near normal central retinal function for at least 3 years, indicating that cones can survive under non-ideal conditions.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Computer Science, Interdisciplinary Applications
Jianfei Liu, Christine Shen, Nancy Aguilera, Catherine Cukras, Robert B. Hufnagel, Wadih M. Zein, Tao Liu, Johnny Tam
Summary: This paper introduces a method called A-GANs, which efficiently generates annotated images by combining ACAM and C-GANs. Experimental results show that it performs well in cell segmentation and synthesizing realistic images.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2021)
Article
Biochemical Research Methods
John P. Giannini, Rongwen Lu, Andrew J. Bower, Robert Fariss, Johnny Tam
Summary: Adaptive optics reflectance-based retinal imaging is a noninvasive imaging technique that allows for easier visualization of subcellular features in ex vivo samples. By using the same imaging modalities as in vivo imaging but with increased resolution, it enables simultaneous imaging and classification of cells and transparent retinal layers.
BIOMEDICAL OPTICS EXPRESS
(2022)
Meeting Abstract
Ophthalmology
Andrew Bower, Tao Liu, Zhuolin Liu, Nancy Aguilera, Joanne Li, Rongwen Lu, John Giannini, Steven Cornelissen, Alfredo Dubra, Daniel Hammer, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Meeting Abstract
Ophthalmology
Joanne Li, Tao Liu, Jianfei Liu, Andrew Bower, John Giannini, Rongwen Lu, Nancy Aguilera, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Meeting Abstract
Ophthalmology
John Giannini, Jianfei Liu, Tao Liu, Nancy Aguilera, Laryssa A. Huryn, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Meeting Abstract
Ophthalmology
Nancy Aguilera, Andrew J. Bower, Tao Liu, Joanne Li, John Giannini, Brian Patrick Brooks, Wadih M. Zein, LaryssaHuryn, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Meeting Abstract
Ophthalmology
Tao Liu, Nancy Aguilera, Andrew J. Bower, Jianfei Liu, Laryssa A. Huryn, Wadih M. Zein, Wendy J. Introne, Robert B. Hufnagel, Brian P. Brooks, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Proceedings Paper
Engineering, Biomedical
Jianfei Liu, Yoo-Jean Han, Tao Liu, Johnny Tam
Summary: Defects in RPE cells can lead to blinding diseases, and the combination of adaptive optics imaging with ICG allows for real-time monitoring. Spatially-aware deep learning networks, incorporating hexagonal arrangement information of RPE cells, improve accuracy in identifying low-contrast cells and may be beneficial for handling images with known spatial constraints.
MEDICAL IMAGING 2020: BIOMEDICAL APPLICATIONS IN MOLECULAR, STRUCTURAL, AND FUNCTIONAL IMAGING
(2021)
Article
Computer Science, Information Systems
Jianfei Liu, Yoo-Jean Han, Tao Liu, Nancy Aguilera, Johnny Tam
IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS
(2020)
Meeting Abstract
Ophthalmology
Joanne Li, Tao Liu, Oliver Flynn, Amy Turriff, Zhuolin Liu, Jianfei Liu, Alfredo Dubra, Robert Hufnagel, Mary A. Johnson, Brian Patrick Brooks, Daniel X. Hammer, Laryssa Huryn, Brett G. Jeffrey, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Jianfei Liu, Christine Shen, Yoo-Jean Han, Nancy Aguilera, Joanne Li, Tao Liu, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Tao Liu, Joanne Li, Nancy Aguilera, Andrew J. Bower, Rongwen Lu, Jianfei Liu, Brian Patrick Brooks, Wadih M. Zein, Laryssa Huryn, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Rongwen Lu, Nancy Aguilera, Tao Liu, John Giannini, Andrew J. Bower, Jianfei Liu, Johnny Tam
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
