Article
Pharmacology & Pharmacy
Tianyang Zhao, Xu Li, Honghao Qian, Xiaohan Miao, Ying Zhu, Jia Wang, Ju Hui, Liting Zhou, Lin Ye
Summary: The study used a microarray platform to investigate the effects of PM2.5 on lipid metabolism in normal arterial intima and advanced atherosclerotic plaque. The results showed changes in the expression levels of certain genes. Atherosclerosis was induced and aggravated in rats through PM2.5 exposure, resulting in damage to the physiological structure of the aorta. PM2.5 exposure also significantly affected the expression levels of certain genes involved in lipid metabolism and the Notch signaling pathway.
Article
Engineering, Biomedical
Muhammad Rizwan, Christopher Ling, Chengyu Guo, Tracy Liu, Jia-Xin Jiang, Christine E. Bear, Shinichiro Ogawa, Molly S. Shoichet
Summary: A defined viscoelastic hyaluronan hydrogel is designed to mimic the stress relaxation rate of liver tissue, promoting cholangiocyte organoid growth. Hydrogel viscoelasticity, YAP signaling, and Notch activation play critical roles in cholangiocyte organogenesis.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Immunology
Shi-Qian Liang, Peng-Hui Li, Yi-Yang Hu, Jun-Long Zhao, Fang-Ze Shao, Fang Kuang, Kai-Xi Ren, Tiao-Xia Wei, Fan Fan, Lei Feng, Hua Han, Hong-Yan Qin
Summary: Yolk sac-derived microglia and peripheral monocyte-derived macrophages play a key role in Parkinson's disease (PD) progression. The Notch signaling pathway mediated by RBP-J is activated in amoeboid microglia, and its disruption alleviates dopaminergic neurodegeneration and improves locomotor activity in a PD mouse model.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Paola Grazioli, Andrea Orlando, Nike Giordano, Claudia Noce, Giovanna Peruzzi, Behnaz Abdollahzadeh, Isabella Screpanti, Antonio Francesco Campese
Summary: This study reveals that dysregulation of the Notch signaling pathway in T cells promotes the production of MDSCs in a T-ALL mouse model. IL-6 is found to mediate the induction of MDSCs in both mouse and human models.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jian Ding, Ming Xu, Wei Du, Zhi-Qiang Fang, Hao Xu, Jing-Jing Liu, Ping Song, Chen Xu, Zhi-Wen Li, Zhen-Sheng Yue, Yu-Wei Ling, Juan-Li Duan, Tao . Kai-Shan, Fei He, Lin Wang
Summary: The Notch signaling pathway in macrophages plays a critical role in the development and progression of NAFLD. Inhibiting Notch signaling in macrophages can attenuate experimental steatohepatitis. Exosomes loaded with RBP-J decoy ODNs have the potential to effectively inhibit Notch signaling in liver macrophages in vivo and ameliorate steatohepatitis or steatosis in NAFLD mice.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Miao Qi, Xinyi Zhao, Runze Fan, Xinying Zhang, Sansan Peng, Dehui Xu, Yanjie Yang
Summary: This study found that plasma treatment can inhibit the engraftment of multiple myeloma in vivo and prolong survival time by suppressing the Notch pathway through ROS regulation. This provides a new approach for plasma treatment of multiple myeloma.
Article
Cell Biology
Xuexia Liu, Qian Li, Zhixin Wang, Fujun Liu
Summary: Cyclophosphamide (CP) treatment leads to reduced serum testosterone levels, impaired sperm quality, and disruption of meiosis and blood-testis barrier integrity in mice. CP induces germ cell apoptosis, inhibits germ cell proliferation, and negatively affects male reproductive function, providing insights for future research on protective strategies against CP toxicity.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Yi Tian Yap, Wei Li, Qi Zhou, Sarah Haj-Diab, Dipanwita Dutta Chowdhury, Asmita Vaishnav, Pamela Harding, David C. Williams, Brian F. Edwards, Jerome F. Strauss, Zhibing Zhang
Summary: SPAG6 and SPAG6L are proteins with different functions, playing important roles in sperm generation, hearing, and immunological synapse.
Article
Genetics & Heredity
Andriy S. Yatsenko, Halyna R. Shcherbata
Summary: This study reveals that multiple modes of Notch signaling activation contribute to the specification of spatial cellular interactions necessary for stem cell niche assembly in Drosophila. It demonstrates the trans-activation of Notch signaling among non-equipotent cells through distant induction, as well as a secondary security mechanism controlled by the soma to ensure the formation of the stem cell niche. These findings advance our understanding of Notch signaling and stem cell niche formation, with implications for stem cell biology and regenerative medicine.
Article
Developmental Biology
Yukino Wada, Hikaru Tsukatani, Chihiro Kuroda, Yurika Miyazaki, Miku Otoshi, Isao Kobayashi
Summary: This study identifies a previously unknown spatiotemporal regulatory mechanism of hematopoietic stem cell specification by somites in zebrafish, in which Jag2b-induced Notch signaling leads to the expression of wnt16 and HSC markers.
Article
Biochemistry & Molecular Biology
Qi-Xin Xu, Wang-Qing Zhang, Xiao-Zheng Liu, Wan-Kun Yan, Lei Lu, Shan-Shan Song, Shu-Wen Wei, Ying-Nan Liu, Jin-Wen Kang, Ren-Wei Su
Summary: Studies suggest that the Notch signaling pathway may play a role in upregulating collagen expression in endometrial fibrosis. By mimicking fibrosis in the mouse uterus, it was found that Notch receptors were upregulated 3 days post-surgery, and most fibril-forming collagens began to upregulate 6 days post-surgery. Inducing intrauterine adhesion in N1ICD-OEx mice significantly enhanced collagen expression and fibrosis levels.
Article
Developmental Biology
Henning J. Falk, Takehito Tomita, Gregor Monke, Katie McDole, Alexander Aulehla
Summary: Coordination of signaling activities in space and time is crucial for embryonic development. By studying the dynamics of the segmentation clock gene Lfng during mouse embryo development, we found similarities to previous findings in zebrafish embryos and observed a gradual establishment of wave patterns during the initial oscillation cycles.
Article
Developmental Biology
Mingyao Yang, Wenzhi Ma, Jon Oatley, Wan-Sheng Liu
Summary: This study reveals the involvement of PRAMEL1 in the initiation and maintenance of spermatogenesis by regulating the responsiveness of prospermatogonia to retinoic acid (RA) and their homing process during spermatogenesis initiation. Pramel1 deficiency leads to altered fecundity in males and the occurrence of a Sertoli cell-only phenotype.
Article
Toxicology
Dou Yang, Juhua Xiao, Mengqi Wan, Jieping Liu, Ling Huang, Xue Li, Li Zhang, Fasheng Liu, Desheng Liang, Yongliang Zheng, Baogang Xie, Xinjun Liao, Guanghua Xiong, Huiqiang Lu, Zigang Cao, Shouhua Zhang
Summary: This study investigated the effects of roxadustat on early embryonic development using zebrafish. The results showed that roxadustat exposure caused abnormal embryonic development and liver metabolic imbalance in zebrafish embryos. Additionally, roxadustat down-regulated Notch signaling, which contributed to hepatotoxicity. This research provides valuable insights for the clinical use of roxadustat.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Reproductive Biology
Bongki Kim, Sylvie Breton
Summary: This study aimed to clarify the role of the MAPK/ERK signaling pathway in regulating the expression and localization of Cx43 in the mouse proximal epididymis. The results showed that PD325901 treatment significantly increased the localization of Cx43 on tight junctions, where it colocalized with ZO-1.
BIOLOGY OF REPRODUCTION
(2022)
Article
Microbiology
Marta F. Silva, Ana L. Pereira, Maria J. Fraqueza, Goncalo Pereira, Luisa Mateus, Luis Lopes-da-Costa, Elisabete Silva
Summary: This study evaluated the virulence potential and biovar characteristics of Cfv isolates, revealing the absence of putative VF genes in 92% of isolates and the invalidation of parA gene for diagnostic purposes. Three novel MLST sequence types were identified. In addition, WGS identified antimicrobial resistance-related genes and segregated the Cfv and Cfvi strains into different clusters based on SNP and accessory protein families analysis.
Article
Developmental Biology
Mariana R. Batista, Patricia Diniz, Daniel Murta, Ana Torres, Luis Lopes-da-Costa, Elisabete Silva
Summary: This study investigated the role of Notch and Wnt cell signaling interplay in the mouse early embryo, and found that they regulate the pace of embryo kinetics by respectively speeding and braking development. Notch and Wnt blockades had significant opposing effects on developmental kinetics, with Notch blockade retarding while Wnt blockade fastening development. The double blockade induced more severe phenotypes than those expected from the cumulative effects of single blockades, and significantly decreased fetal development to term.
Article
Agriculture, Dairy & Animal Science
Pedro Pinto-Bravo, Maria Rosa Rebordao, Ana Amaral, Carina Fernandes, Antonio Galvao, Elisabete Silva, Pedro Pessa-Santos, Graca Alexandre-Pires, Rosario P. Roberto da Costa, Dariusz J. Skarzynski, Graca Ferreira-Dias
Summary: The oviduct provides ideal conditions for fertilization and early embryonic development, with proper vascularization being essential for its physiological function. This study examined differences in microvascular density across different portions of the equine oviduct and found that angiogenic factors and their receptors are expressed in all studied regions, in line with microvascular patterns.
Article
Biotechnology & Applied Microbiology
Goncalo Pereira, Yongzhi Guo, Elisabete Silva, Claudia Bevilacqua, Gilles Charpigny, Luis Lopes-da-Costa, Patrice Humblot
Summary: The specific transcriptomic profiles of endometrial LE, GE, and ST cells undergo changes under progesterone regulation, with progesterone exerting an anti-proliferative effect on epithelial cells and downregulating estrogen receptor 1 (ESR1) and oxytocin receptor (OXTR) in glandular epithelial (GE) and stromal (ST) cells.
Article
Agriculture, Dairy & Animal Science
Goncalo Pereira, Patricia Simoes, Ricardo Bexiga, Elisabete Silva, Luisa Mateus, Tatiane Fernandes, Susana P. Alves, Rui J. B. Bessa, Luis Lopes-da-Costa
Summary: Supplementing high-yielding dairy cows with fat, especially those enriched with n-3 PUFA, can improve energy balance and immune status, leading to enhanced overall health and reproductive efficiency. The study found a positive linear relationship between rumen-protected linseed fat intake and plasma n-3 PUFA concentrations, which influenced adiponectin expression and reproductive parameters.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Veterinary Sciences
Ana Catarina Neto da Silva, Ana Luisa Costa, Ana Teixeira, Joana Alpoim-Moreira, Carina Fernandes, Maria Joao Fradinho, Maria Rosa Rebordao, Elisabete Silva, Jose Ferreira da Silva, Miguel Bliebernicht, Graca Alexandre-Pires, Graca Ferreira-Dias
Summary: Increasing collagen fibers in the placenta of older mares may affect fertility, but not neonate weight. Older mares may develop a heavier, more vascularized functional placenta with more collagen, providing adequate nutrition to the equine fetus.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jorge M. B. G. A. Pimenta, Virginia M. R. Pires, Sofia Nolasco, Pedro Castelo-Branco, Carla C. Marques, Joana Apolonio, Rita Azevedo, Monica T. Fernandes, Luis Lopes-da-Costa, Jose Prates, Rosa M. L. N. Pereira
Summary: Prion proteins have been extensively studied in relation to male fertility, but their role in female fertility remains relatively unexplored. This study investigated the impact of SPRN and PRNP genes on bovine follicular steroidogenesis pathways. The findings suggest a potential interaction between SPRN and PRNP regulation, as well as the influence of SPRN expression on CYP11A1 and progesterone levels. These results provide new insights into the role of these genes in ovarian steroidogenesis and female reproductive physiology.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Goncalo Perelra, Yongzhi Guo, Elisabete Silva, Marta Filipa Silva, Claudia Bevilacqua, Gilles Charpigny, Luis Lopes-da-Costa, Patrice Humblot
Summary: This study discovered distinct transcription profiles of endometrial stromal, glandular, and luminal epithelial cells associated with different endometrial health statuses. Recovery or persistence of inflammation was related to gene expression patterns involved in immune function, tissue remodeling, cell adhesion, and uterine receptivity.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
C. Esteves, P. Fareleira, M. A. Castelo-Branco, I. G. Lopes, M. Mota, D. Murta, R. Menino
Summary: This study evaluated the agronomic value of black soldier fly larvae frass (BSFF) as an organic fertiliser in short-cycle crops. Although BSFF was not effective as an exclusive fertiliser for short-cycle crops, when combined with mineral fertilisation, it improved soil fertility and crop production sustainability.
JOURNAL OF INSECTS AS FOOD AND FEED
(2022)
Article
Infectious Diseases
Rafaela Zina, Eva Cunha, Isa Serrano, Elisabete Silva, Luis Tavares, Manuela Oliveira
Summary: Diabetic foot ulcers (DFU) caused by P. aeruginosa infections are a global public health concern. This study investigated the inhibitory effect of nisin Z on persister cells of P. aeruginosa, which are highly tolerant to antibiotics. Nisin Z showed a high inhibitory effect on persister cells in planktonic suspensions, but could not eradicate them in established biofilms. Transcriptome analysis revealed that persistence was associated with downregulation of genes related to metabolic processes, cell wall synthesis, and dysregulation of stress response and biofilm formation. Nisin Z could be considered as a potential complementary therapy for treating P. aeruginosa DFU, but should be used early or after wound debridement.
Article
Biotechnology & Applied Microbiology
Ana Rita Azevedo, Ana Sofia Pais, Teresa Almeida-Santos, Virginia M. R. Pires, Pedro Pessa, Carla C. Marques, Sofia Nolasco, Pedro Castelo-Branco, Jose A. M. Prates, Luis Lopes-da-Costa, Mafalda Laranjo, Maria Filomena Botelho, Rosa M. L. N. Pereira, Jorge M. B. G. A. Pimenta
Summary: This study evaluated and compared the application of honey and other strategies to improve ovarian grafts' viability. The results showed that honey stimulated angiogenesis and cell proliferation, while inducing cellular apoptosis within a healthy cellular turnover pathway. Therefore, honey could be considered as an effective and cost-efficient strategy to improve ovarian tissue transplantation.
BIOENGINEERING-BASEL
(2022)
Article
Veterinary Sciences
Vera Fernandes, Eva Cunha, Telmo Nunes, Elisabete Silva, Luis Tavares, Luisa Mateus, Manuela Oliveira
Summary: This study aimed to determine the susceptibility profile of E. coli isolates obtained from healthy and pyometra-presenting dogs and compare the application of different antimicrobial susceptibility guidelines. The results emphasize the importance of determining the antimicrobial susceptibility profile in veterinary isolates and the necessity of using standardized and validated testing methods and harmonized interpretive criteria.
VETERINARY SCIENCES
(2022)