Article
Immunology
Rachel E. Crossland, Jean Norden, Sakhila Ghimire, Mateja Kralj Juric, Kim F. Pearce, Clare Lendrem, Matthew Collin, Eva Mischak-Weissinger, Ernst Holler, Hildegard T. Greinix, Anne M. Dickinson
Summary: The study found that miR-155 and miR-146a were upregulated in gastrointestinal and skin tissue samples of aGvHD patients, and their expression levels in serum and urine samples were also associated with the risk of developing aGvHD. These results support the potential of miR-155 and miR-146a as biomarkers for aGvHD, but further investigation is needed to understand their role in generalized inflammation and specific pathophysiology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mona A. Eldosoky, Reham Hammad, Asmaa A. Elmadbouly, Reda Badr Aglan, Sherihan G. Abdel-Hamid, Mohamed Alboraie, Donia Ahmed Hassan, Mohamed A. Shaheen, Areej Rushdi, Reem M. Ahmed, Alzahra Abdelbadea, Neamat A. Abdelmageed, Ahmed Elshafei, Elham Ali, Omaima I. Abo-Elkheir, Samy Zaky, Nadia M. Hamdy, Claude Lambert
Summary: Early HCC diagnosis is challenging, especially for AFP-negative cases. miRNA profiles may serve as potential molecular markers for HCC. This study aimed to evaluate the expression levels of four miRNAs (hsa-miR-21-5p, hsa-miR-155-5p, hsa-miR-192-5p, and hsa-miR-199a-5p) as biomarkers for HCC in CHCV patients with liver cirrhosis, particularly AFP-negative HCC cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Rukiye Tekdemir, Yavuz Selvi, Kursat Altinbas, Nadir Kocak
Summary: This study found that miRNAs play an important role in the pathogenesis of bipolar disorder and may serve as biomarkers for diagnosis and screening of the disorder.
JOURNAL OF AFFECTIVE DISORDERS
(2022)
Article
Oncology
Reham Hammad, Mona A. Eldosoky, Asmaa A. Elmadbouly, Reda Badr Aglan, Sherihan G. Abdelhamid, Samy Zaky, Elham Ali, Fatma El-Zahraa Abd El Hakam, Alshaimaa M. Mosaad, Neamat A. Abdelmageed, Fatma M. Kotb, Hend G. Kotb, Ahmed A. Hady, Omaima I. Abo-Elkheir, Sandy Kujumdshiev, Ulrich Sack, Claude Lambert, Nadia M. Hamdy
Summary: The authors aim to investigate the altered monocytes subsets distribution in liver cirrhosis (LC) and subsequent hepatocellular carcinoma (HCC) in association with the expression level of plasma Homo sapiens (has)-miR-21-5p and hsa-miR-155-5p. This is a step towards non-protein coding (nc) RNA precision medicine based on the immune perturbation manifested as altered monocytes distribution, on top of LC and HCC.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Oncology
Reham Hammad, Mona A. Eldosoky, Asmaa A. Elmadbouly, Reda Badr Aglan, Sherihan G. AbdelHamid, Samy Zaky, Elham Ali, Fatma El-Zahraa Abd El Hakam, Alshaimaa M. Mosaad, Neamat A. Abdelmageed, Fatma M. Kotb, Hend G. Kotb, Ahmed A. Hady, Omaima I. Abo-Elkheir, Sandy Kujumdshiev, Ulrich Sack, Claude Lambert, Nadia M. Hamdy
Summary: The purpose of this study was to investigate the correlation between altered monocyte subsets distribution in liver cirrhosis (LC) and hepatocellular carcinoma (HCC) and the expression level of plasma Homo sapiens (has)-miR-21-5p and hsa-miR-155-5p. The results showed that the expression of hsa-miR-21-5p was positively correlated with intermediate monocytes, while the expression of hsa-miR-155-5p was negatively correlated with non-classical monocytes. Combining the frequency of intermediate monocytes and hsa-miR-21 could be a sensitive indicator of LC progression to HCC.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Medicine, Research & Experimental
Jose L. Sanchez-Gloria, Roxana Carbo, Mabel Buelna-Chontal, Horacio Osorio-Alonso, Adrian Henandez-Diazcouder, Rosa Luz de la Fuente-leon, Julio Sandoval, Fausto Sanchez, I. Rubio-Gayosso, Fausto Sanchez-Munoz
Summary: Chronic cold exposure exacerbates MCT-induced pulmonary arterial hypertension in rats, resulting in increased levels of inflammatory markers and miRNAs in plasma EVs and lungs.
Article
Biochemistry & Molecular Biology
Yi-Yung Hung, Chen-Kai Chou, Yi-Chien Yang, Hung-Chun Fu, El-Wui Loh, Hong-Yo Kang
Summary: This study found that in patients with major depressive disorder, the expression levels of miR-146a in exosomes were higher, and patients who achieved remission showed lower levels of various microRNAs before treatment and increased levels after treatment. Let-7e, miR-145, and miR-146a showed acceptable discrimination between the remission and non-remission groups.
Article
Multidisciplinary Sciences
Min Liu, Fei Mo, Xiaohan Song, Yun He, Yan Yuan, Jiaoyan Yan, Ye Yang, Jian Huang, Shu Zhang
Summary: The study utilized comprehensive bioinformatics analysis and experimental validation to identify hsa-miR-21-5p as significantly up-regulated in breast cancer tissues, cells, and exosomes. Further investigations confirmed the diagnostic value of plasma exosomal hsa-miR-21-5p in distinguishing healthy individuals from breast cancer patients, with a sensitivity of 86.7% and specificity of 93.3%.
Article
Biochemistry & Molecular Biology
Ayaka Koma, Shunichi Asai, Chikashi Minemura, Sachi Oshima, Takashi Kinoshita, Naoko Kikkawa, Keiichi Koshizuka, Shogo Moriya, Atsushi Kasamatsu, Toyoyuki Hanazawa, Katsuhiro Uzawa, Naohiko Seki
Summary: The study identified downregulated miR-139 in head and neck squamous cell carcinoma and demonstrated its role in attenuating cancer cell aggressiveness by regulating GNA12 and OLR1 expression, which are associated with patient survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jovana Despotovic, Sandra Dragicevic, Aleksandra Nikolic
Summary: This study examined the impact of chemotherapy on TGF-beta signaling and related miRNAs in mCRC, finding time-dependent alterations in the expression of several miRNAs in SW620 cells. The long-term downregulation pattern of hsa-miR-93-5p and hsa-miR-17-5p could potentially serve as biomarkers for monitoring treatment response in mCRC patients undergoing combined chemotherapy.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Immunology
Yeuni Yu, Sohee Park, Hansong Lee, Eun Jung Kwon, Hae Ryoun Park, Yun Hak Kim, Seung-Geun Lee
Summary: This study aimed to develop potential biomarkers for rheumatoid arthritis (RA) by sequencing small non-coding RNA (sncRNA) in circulating exosomes. The expression levels of hsa-miR-335-5p and hsa-miR-486-5p were found to be higher in RA patients compared to healthy controls, while the expression of the SRSF4 gene was decreased. These findings suggest that circulating exosomal miRNAs and SRSF4 could serve as valuable biomarkers for RA.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Zixu Bao, Ning Zhang, Wanxiang Niu, Maolin Mu, Xiaoming Zhang, Shanshan Hu, Chaoshi Niu
Summary: Exosomes released from glioma stem-like cells (GSCs) containing miR-155-5p can be horizontally transferred to surrounding glioma cells, promoting tumor progression and mesenchymal transition by targeting ACOT12. High expression of miR-155-5p in plasma exosomes is associated with glioma diagnosis and grading, suggesting its potential as a predictive biomarker for glioma.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Neha Pandey, Meghana Rastogi, Sunit K. Singh
Summary: This study highlights the role of hsa-miR-21-5p in regulating pro-inflammatory genes in CHPV-infected human microglial cells. Upregulation of hsa-miR-21-5p during CHPV infection leads to downregulation of PTEN and subsequent phosphorylation of AKT and NF-kappa Bp65 in microglial cells. Overexpression of hsa-miR-21-5p further promotes AKT and NF-kappa Bp65 phosphorylation, while inhibition of hsa-miR-21-5p restores expression levels.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Guowen Wang, Xuefang Tao, Linlin Peng
Summary: The miR-155-5p/PYGL pathway plays a crucial role in pulmonary arterial hypertension (PAH). Increased levels of miR-155-5p enhance cell proliferation, migration, and cell cycle progression in hypoxia-induced pulmonary artery smooth muscle cells (PASMCs) by directly targeting PYGL.
Article
Biochemistry & Molecular Biology
Rongzhi Li, Tomomi Sano, Akiko Mizokami, Takao Fukuda, Takanori Shinjo, Misaki Iwashita, Akiko Yamashita, Terukazu Sanui, Yusuke Nakatsu, Yusuke Sotomaru, Tomoichiro Asano, Takashi Kanematsu, Fusanori Nishimura
Summary: A well-tuned inflammatory response is crucial, and dysregulation of nuclear factor-kappa B (NF-kappa B) is associated with immune-related diseases. This study identified miR-582-5p as a miRNA that modulates NF-kappa B signaling during inflammation. It was found that miR-582-5p targets S-phase kinase-associated protein 1 (SKP1) and attenuates the NF-kappa B pathway, leading to reduced production of pro-inflammatory cytokines. This suggests that exogenous miR-582-5p could be a potential therapeutic strategy for inflammatory and immune diseases.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Fang Wang, Farrah El-Saafin, Tao Ye, Matthieu Stierle, Luc Negroni, Matej Durik, Veronique Fischer, Didier Devys, Stephane D. Vincent, Laszlo Tora
Summary: The co-activator complex SAGA, containing USP22 and ATXN7L3, is essential for normal embryonic development in mice, with Atxn7l3(-/-) embryos showing developmental delay at E7.5 and Usp22(-/-) embryos dying at E14.5. Deubiquitylation of histone H2Bub1 by these proteins does not directly regulate global Pol II transcription elongation.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Eva Erdmann, Pauline Ould Madi Berthelemy, Felicie Cottard, Charlotte Zoe Angel, Edwige Schreyer, Tao Ye, Bastien Morlet, Luc Negroni, Bruno Kieffer, Jocelyn Ceraline
Summary: Androgen receptor (AR) signaling is a key therapeutic target for hormone-naive-advanced prostate cancer (PCa) and castration-resistant PCa (CRPC). Recent studies have identified constitutively active AR variants in CRPC, which lack the ligand-binding domain. These variants contribute to tumor progression by affecting gene expression. However, the specific mechanism of action of these variants compared to wild-type AR (AR-WT) remains unclear. Transcriptome analysis and functional enrichment analysis revealed that AR-WT has a transcriptional repression function associated with genes involved in cell adhesion and epithelial-to-mesenchymal transition. This repression function is pathologically lost in AR variants found in PCa.
MOLECULAR ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xu Zhao, Ivo A. Hendriks, Stephanie Le Gras, Tao Ye, Lucia Ramos-Alonso, Aurelie Nguea P, Guro Flor Lien, Fatemeh Ghasemi, Arne Klungland, Bernard Jost, Jorrit M. Enserink, Michael L. Nielsen, Pierre Chymkowitch
Summary: Sumoylation pathway plays a crucial role in adipocyte differentiation, supporting both the establishment of mature adipocyte transcriptional program and rewiring of transcriptional networks.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Hematology
Marie Passet, Rathana Kim, Stephanie Gachet, Francois Sigaux, Julie Chaumeil, Ava Galland, Thomas Sexton, Samuel Quentin, Lucie Hernandez, Lise Larcher, Hugo Bergugnat, Tao Ye, Nezih Karasu, Aurelie Caye, Beate Heizmann, Isabelle Duluc, Patrice Chevallier, Philippe Rousselot, Francoise Huguet, Thibaut Leguay, Mathilde Hunault, Francoise Pflumio, Jean-Noel Freund, Camille Lobry, Veronique Lheritier, Herve Dombret, Claire Domon-Dell, Jean Soulier, Nicolas Boissel, Emmanuelle Clappier
Summary: A novel subtype of B-cell acute lymphoblastic leukemia (B-ALL) characterized by CDX2 cis-deregulation and UBTF::ATXN7L3 fusion was identified in young adults, showing poor response to treatment and high risk of relapse. This subtype represents a high-risk disease entity in adults with unique genetic alterations.
Article
Multidisciplinary Sciences
Sophie Quintin, Theo Aspert, Tao Ye, Gilles Charvin
Summary: Environmental oxidative stress poses a threat to cellular integrity and therefore needs to be avoided by living organisms. However, our understanding of how organisms perceive environmental oxidative stress is limited. In this study, using microfluidics, researchers discovered that the tail phasmid PHA neurons in C. elegans function as oxidative stress sensing neurons, similar to 12 pharyngeal neurons. Interestingly, the two types of neurons displayed different responses to H2O2 and light. The study also identified the receptors GUR-3 and LITE-1 as mediators of H2O2 signaling in the neurons, with the peroxiredoxin PRDX-2 playing an essential role in both.
Article
Cell Biology
Justine De Azevedo, Jana Mourtada, Cyril Bour, Veronique Devignot, Philippe Schultz, Christian Borel, Erwan Pencreach, Georg Mellitzer, Christian Gaiddon, Alain C. Jung
Summary: The EXTREME protocol or cetuximab alone are capable of eliciting an anti-tumor immune response in HNSCC under conditions of moderate apoptosis induction.
Article
Oncology
A. Venkatasamy, E. Guerin, W. Reichardt, V. Devignot, M. P. Chenard, L. Miguet, B. Romain, A. C. Jung, I. Gross, C. Gaiddon, G. Mellitzer
Summary: By establishing patient-derived xenograft (PDX) models and using a morpho-functional approach combining high-resolution imaging with molecular analyses, we have successfully studied the complex pathology of gastric cancer. We have characterized the expression of important therapeutic biomarkers and observed the presence of muscle atrophy in the tumor ecosystem.
Article
Biology
Mira Brazane, Dilyana G. Dimitrova, Julien Pigeon, Chiara Paolantoni, Tao Ye, Virginie Marchand, Bruno Da Silva, Elise Schaefer, Margarita T. Angelova, Zornitza Stark, Martin Delatycki, Tracy Dudding-Byth, Jozef Gecz, Pierre-Yves Placais, Laure Teysset, Thomas Preat, Amelie Piton, Bassem A. Hassan, Jean-Yves Roignant, Yuri Motorin, Clement Carre
Summary: This study reports a novel pathogenic variant of FTSJ1 associated with X-linked intellectual disability (XLID). Using RiboMethSeq analysis, the researchers identified ribose methylation on all human tRNAs and novel targets. Transcriptome analysis revealed deregulation of genes associated with intellectual disability and cancers. The study also uncovered changes in the miRNA population and demonstrated defects in neuron morphology associated with FTSJ1 depletion.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biochemistry & Molecular Biology
Marta Martinez-Alonso, Albert Gandioso, Chloe Thibaudeau, Xue Qin, Philippe Arnoux, Nurikamal Demeubayeva, Vincent Guerineau, Celine Frochot, Alain C. Jung, Christian Gaiddon, Gilles Gasser
Summary: A novel Ru(II) cyclometalated photosensitizer, Ru-NH2, and its bioconjugates, Ru-Mal-CTX and Ru-BAA-CTX, were synthesized and characterized. Ru-NH2 showed absorption maxima around 580 nm and generated singlet oxygen upon light irradiation. In vitro experiments demonstrated the excellent phototoxicity of Ru-NH2 towards CT-26 and SQ20B cells under specific light conditions.
Article
Biochemistry & Molecular Biology
Helene Neyret-Kahn, Jacqueline Fontugne, Xiang Yu Meng, Clarice S. Groeneveld, Luc Cabel, Tao Ye, Elodie Guyon, Cle'mentine Krucker, Florent Dufour, Elodie Chapeaublanc, Audrey Rapinat, Daniel Jeffery, Laura Tanguy, Victoria Dixon, Yann Neuzillet, Thierry Lebret, David Gentien, Irwin Davidson, Yves Allory, Isabelle Bernard-Pierrot, Francois Radvanyi
Summary: Muscle-invasive bladder cancer (BLCA) has distinct molecular and clinical characteristics, with Luminal and Basal subtypes showing different gene expression profiles. In this study, the researchers established an integrated epigenetic map of BLCA, revealing the link between subtype and epigenetic control. They identified the activated super-enhancers and discovered the super-enhancer-regulated networks of candidate master transcription factors for Luminal and Basal subgroups. The study provides insights into the epigenetic regulation of BLCA and potential therapeutic targets.
Article
Multidisciplinary Sciences
Nisha Misra, Manohar Damara, Tao Ye, Pierre Chambon
Summary: This study reveals a tight functional relationship between the circadian clock and DNA demethylation-methylation of deoxyCpG islands. It is discovered that circadian clock-controlled output genes contain a specific intronic deoxyCpG island, whose demethylation-methylation is controlled by the circadian clock. These intronic islands undergo demethylation during transcriptional activation and store activating components for the initiation of transcription of their cognate pre-mRNAs. The study demonstrates how intronic deoxyCpG islands cooperate with cognate enhancers to boost circadian gene transcription and expression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Rheumatology
Renaud Felten, Tao Ye, Cedric Schleiss, Benno Schwikowski, Jean Sibilia, Fanny Monneaux, Helene Dumortier, Roland Jonsson, Christopher Lessard, Fai Ng, Tsutomu Takeuchi, Xavier Mariette, Jacques-Eric Gottenberg
Summary: This study analyzed the gene expression differences between pSS patients and healthy controls and identified 11 potential candidate drugs, including histone deacetylases and PI3K inhibitors. Additionally, 12 genes with a pSS-like profile and 23 genes with a pSS-revert profile were identified, most of which were interferon-regulated.
Article
Biochemistry & Molecular Biology
Ombline Conrad, Mickael Burgy, Sophie Foppolo, Aude Jehl, Alicia Thiery, Sebastien Guihard, Romain Vauchelles, Alain C. C. Jung, Jana Mourtada, Christine Macabre, Sonia Ledrappier, Marie-Pierre Chenard, Mihaela-Alina Onea, Aurelien Danic, Thomas Dourlhes, Claire Thibault, Philippe Schultz, Monique Dontenwill, Sophie Martin
Summary: The expression levels of the 3p passenger strands of miR-30a and miR-30e are correlated with the behavior and prognosis of head and neck squamous cell carcinoma. Low expression of miR-30a and miR-30e is associated with adverse prognosis and can potentially be used as biomarkers for diagnosis and treatment of head and neck squamous cell carcinoma. Additionally, miR-30a and miR-30e expression can affect tumor cell behavior and the tumor microenvironment, potentially improving treatment outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ferdinand Marletaz, Arnaud Couloux, Julie Poulain, Karine Labadie, Corinne Da Silva, Sophie Mangenot, Benjamin Noel, Albert J. Poustka, Philippe Dru, Cinta Pegueroles, Marco Borra, Elijah K. Lowe, Guy Lhomond, Lydia Besnardeau, Stephanie Le Gras, Tao Ye, Daria Gavriouchkina, Roberta Russo, Caterina Costa, Francesca Zito, Letizia Anello, Aldo Nicosia, Maria Antonietta Ragusa, Marta Pascual, M. Dolores Molina, Aline Chessel, Marta Di Carlo, Xavier Turon, Richard R. Copley, Jean-Yves Exposito, Pedro Martinez, Vincenzo Cavalieri, Smadar Ben Tabou de Leon, Jenifer Croce, Paola Oliveri, Valeria Matranga, Maria Di Bernardo, Julia Morales, Patrick Cormier, Anne-Marie Genevieve, Jean Marc Aury, Valerie Barbe, Patrick Wincker, Maria Ina Arnone, Christian Gache, Thierry Lepage
Summary: Through the analysis of genome assembly and gene expression in sea urchins, we have identified unique characteristics in their chromosomal structure and gene duplication. Additionally, we have discovered conserved gene-regulatory modules between sea urchins and chordates. These findings contribute to a better understanding of the evolutionary mechanisms behind development.
Article
Cell Biology
Anna Niewiadomska-Cimicka, Antoine Hache, Stephanie Le Gras, Celine Keime, Tao Ye, Aurelie Eisenmann, Imen Harichane, Michel J. J. Roux, Nadia Messaddeq, Emmanuelle Clerin, Thierry Leveillard, Yvon Trottier
Summary: SCA7 leads to reduced active transcription marks at gene promoters in the retina, with a preferential downregulation of highly expressed photoreceptor genes. The affected photoreceptor genes show altered histone acetylation, enhancer-related non-coding RNA expression, and increased RNA polymerase II pausing, suggesting impaired superenhancer-related features in SCA7.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)