Article
Microbiology
Sophie Chagneau, Marie-Lou Gaucher, William P. Theriault, Philippe Fravalo, Alexandre Thibodeau
Summary: This study investigates the relationship between two different C. jejuni strains in poultry and their impact on gut colonization and liver spread. The findings suggest that there is both commensalism and competition between the strains, and the mode of colonization and spread differs depending on the strain. Further research is needed to elucidate the mechanisms of commensalism and competition between strains in order to develop appropriate control strategies for farms.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Harald Nothaft, Maria Elisa Perez-Munoz, Tianfu Yang, Abarna V. M. Murugan, Michelle Miller, Daniel Kolarich, Graham S. Plastow, Jens Walter, Christine M. Szymanski
Summary: The study shows that Campylobacter jejuni spreads through contaminated poultry products, and an attenuated Escherichia coli live vaccine strain expressing the C. jejuni N-glycan can reduce the Campylobacter load in poultry, with distinct responder and non-responder outcomes. Research indicates that antibodies from responder birds have higher Campylobacter-opsonophagocytic activity, and transplantation of cecal microbiota from responder birds can enhance vaccine-induced antigen-specific IgY responses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Ross C. Beier, J. Allen Byrd, Kathleen Andrews, Denise Caldwell, Tawni L. Crippen, Robin C. Anderson, David J. Nisbet
Summary: The study found that Campylobacter jejuni strains from broiler chicken house environments had low resistance to tetracycline, ciprofloxacin, and nalidixic acid, but resistance to disinfectants like triclosan. Didecyldimethylammonium chloride was the most effective component against C. jejuni among various disinfectants.
Article
Multidisciplinary Sciences
Yuli Melisa Sierra-Arguello, Gustavo Perdoncini, Laura Beatriz Rodrigues, Luciana Ruschel dos Santos, Karen Apellanis Borges, Thales Quedi Furian, Carlos Tadeu Pippi Salle, Hamilton Luiz de Souza Moraes, Marcos Jose Pereira Gomes, Vladimir Pinheiro do Nascimento
Summary: Campylobacter jejuni is a common cause of foodborne illnesses worldwide, with limited research on strains isolated from Latin-American countries. This study in Southern Brazil identified 140 C. jejuni strains from various poultry sources and analyzed their virulence markers, providing insight into factors related to the occurrence of this pathogen and their epidemiology. The PCR analysis revealed 25 different virulence profiles, with certain combinations of virulence genes being more common among the strains.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Katarina Simunovic, Orhan Sahin, Andi Erega, Polonca Stefanic, Qijing Zhang, Ines Mandic Mulec, Sonja Smole Mozina, Anja Klancnik
Summary: Campylobacter jejuni is the main cause of bacterial gastroenteritis worldwide, with poultry serving as a major source of infection. A study found that Bacillus subtilis PS-216 strain showed strong antimicrobial activity against C. jejuni isolates in vitro co-cultures, making it a promising probiotic for reducing C. jejuni colonization in poultry. In animal experiments, continuous treatment with B. subtilis PS-216 spores in drinking water significantly reduced Campylobacter counts in broiler chickens' caeca and improved their weight gain.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
G. Douglas Inglis, Nahal Ramezani, Eduardo N. Taboada, Valerie F. Boras, Richard R. E. Uwiera
Summary: This study highlights the importance of longitudinal examination of Campylobacter jejuni subtypes in the poultry production continuum to understand transmission mechanisms and foodborne risks. It shows that a limited number of C. jejuni subtypes from a small number of farms contaminate the abattoir, leading to a high prevalence of contaminated carcasses with diverse subtypes, which are transferred to retail settings. However, only a subset of these strains pose a high risk to human health.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Benjamin Duque, Sandrine Reze, Albert Rossero, Jeanne-Marie Membre, Sandrine Guillou, Nabila Haddad
Summary: Broiler meat is considered a significant source of Campylobacter jejuni, a foodborne pathogen. Stress conditions during slaughtering can affect bacterial adaptation and resistance, leading to changes in gene expression. RT-qPCR was used to measure gene expression in 44 C. jejuni genes after thermal stresses, revealing differential expression in 26 genes. The study provides insights into the molecular mechanisms of bacterial stress response in real-life processing conditions.
Article
Food Science & Technology
Daniel Eriksson, Ella Rahlen, Emma Bergenkvist, Moa Skarin, Lise-Lotte Fernstrom, Jesper Ryden, Ingrid Hansson
Summary: Most Campylobacter infections in humans are sporadic cases connected to private households. Chicken meat, believed to be the main source of human exposure, poses cross-contamination risks in the kitchen. Freezing can reduce Campylobacter concentrations on chicken meat. Different Campylobacter sequence types have varying abilities to withstand freezing stress. Campylobacter remaining on hands after washing and on cutting boards after wiping are likely sources of cross-contamination in the kitchen.
Article
Agriculture, Dairy & Animal Science
Pongthorn Pumtang-on, Timothy J. Mahony, Rodney A. Hill, Anthony Pavic, Thiru Vanniasinkam
Summary: Campylobacter spp. contamination in poultry products is a global concern for foodborne illnesses. Understanding the factors driving contamination and investigating genetic diversity can help in developing better intervention strategies. This study found potential sources of Campylobacter jejuni and Campylobacter coli colonization in free-range broiler farms, enhancing knowledge for controlling this foodborne pathogen effectively.
Article
Genetics & Heredity
Julia Hankel, Sophie Kittler, Bussarakam Chuppava, Eric Galvez, Till Strowig, Andre Becker, Maren von Koeckritz Blickwede, Madeleine Plotz, Christian Visscher
Summary: This study aimed to investigate the interactions between the host microbiota and Campylobacter jejuni in broiler chickens. The results showed that the infection status of C. jejuni significantly influenced the microbial composition in the chicken's intestines, with the presence of certain bacterial families being associated with C. jejuni infection. The Christensenellaceae R-7 group was found to have a negative correlation with the colonization of C. jejuni.
MICROBIAL GENOMICS
(2022)
Article
Engineering, Chemical
Ali Al-Sakkaf
Summary: This study aimed to determine the microbial kinetic parameters in chicken under dynamic temperature for campylobacteriosis strains in New Zealand. The obtained D and z values were consistent with international isothermal data, indicating that the high rate of campylobacteriosis in New Zealand is not due to the emergence of more heat resistant strains.
JOURNAL OF FOOD ENGINEERING
(2021)
Article
Veterinary Sciences
Yang-Chi Fan, Yi-Tei Wu, Yi-Hsieng Samuel Wu, Chia-Lan Wang, Chung-Hsi Chou, Yi-Chen Chen, Hsiang-Jung Tsai
Summary: The addition of trehalose to broiler feed does not have a negative impact on broiler farming, but it can increase the Lactobacillus counts in the chicken gut, improving gut health. This research has significance in studying the characteristics of trehalose and finding alternatives to antibiotics and antimicrobial growth promoters in animal feed.
VETERINARY SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Taketoshi Iwata, Ayako Watanabe-Yanai, Yukino Tamamura-Andoh, Nobuo Arai, Masato Akiba, Masahiro Kusumoto
Summary: Campylobacter jejuni is a leading cause of foodborne bacterial gastroenteritis worldwide, and raw or undercooked chicken meat is considered the major source of human campylobacteriosis. We identified 36 compounds with inhibitory effects on C. jejuni, and among them, tryptanthrin (TRP) showed bactericidal activity against C. jejuni. TRP can effectively reduce the level of C. jejuni infection in chicken farms.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Virology
Daniela D'Angelantonio, Silvia Scattolini, Arianna Boni, Diana Neri, Gabriella Di Serafino, Philippa Connerton, Ian Connerton, Francesco Pomilio, Elisabetta Di Giannatale, Giacomo Migliorati, Giuseppe Aprea
Summary: The study assessed the efficacy of two field-isolated bacteriophages against an antimicrobial-resistant Campylobacter jejuni strain in experimental infections. A two-step phage application successfully reduced the Campylobacter load in broilers, showcasing the potential of phage therapy in controlling Campylobacter in poultry.
Article
Zoology
Abdul Muhaimin Wahab, Basit Zeshan, Naveed Ahmed, Muhammad Afzal, Muhammad Naveed
Summary: This study aimed to investigate the prevalence of C. jejuni in broiler chicken farms in Kelantan state, as well as the associated risk factors. By identifying and addressing these risk factors, control measures can be implemented to reduce the occurrence of this pathogen in poultry production.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)
Article
Chemistry, Medicinal
Silvia Wuerstle, Jana Stender, Jens Andre Hammerl, Kilian Vogele, Kathrin Rothe, Christian Willy, Joachim Jakob Bugert
Summary: This study aims to establish an efficient personalized phage delivery pipeline and provide guidelines and evaluation tools. By adjusting the components and set points of the pipeline, the efficiency of the process was successfully improved. This is of great importance for treating critically ill patients with multidrug-resistant Gram-negative bacterial infections.
Article
Biology
Tobias Lienen, Arne Schnitt, Jens Andre Hammerl, Sven Maurischat, Bernd-Alois Tenhagen
Summary: This study investigated the antimicrobial resistance potential of mammaliicocci isolates from German dairy farms. The findings showed that these isolates carry multiple antimicrobial resistance genes and exhibit resistance to various antibiotics. These mammaliicocci may play a significant role in the transmission of antimicrobial resistance genes.
Article
Biotechnology & Applied Microbiology
Katharina Juraschek, Janina Malekzadah, Burkhard Malorny, Annemarie Kaesbohrer, Stefan Schwarz, Diana Meemken, Jens Andre Hammerl
Summary: This study investigated the occurrence and genetic basis of the fluoroquinolone resistance enhancing determinant qnrB in ESBL-/non-ESBL-producing E. coli. The qnrB-carrying E. coli were found to be highly heterogenic in their multilocus sequence types (STs) and their phenotypic resistance profiles.
Article
Biotechnology & Applied Microbiology
Agnes Perrin-Guyomard, Sophie A. Granier, Jannice Schau Slettemeas, Muna Anjum, Luke Randall, Manal AbuOun, Natalie Pauly, Alexandra Irrgang, Jens Andre Hammerl, Jette Sejer Kjeldgaard, Anette Hammerum, Alessia Franco, Magdalena Skarzynska, Ewelina Kaminska, Dariusz Wasyl, Cindy Dierikx, Stefan Borjesson, Yvon Geurts, Marisa Haenni, Kees Veldman
Summary: This study evaluated the performance of a screening protocol to detect and isolate mcr-positive Escherichia coli and Salmonella spp. from animal caecal content and meat samples. The results showed that the combined method was effective in detecting and isolating E. coli or Salmonella spp. strains harboring different mcr genes, and could potentially be used as a harmonized protocol for screening mcr genes in food-producing animals and food products in Europe.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Food Science & Technology
A. Beterams, T. Tolksdorf, A. Martin, K. Stingl, N. Bandick, F. Reich
Summary: This study aimed to determine the influence of routinely used broiler meat packaging technologies on the change of Campylobacter, Salmonella, and Escherichia coli counts on chicken breast meat. The results showed that MAP packaging resulted in a reduction of thermophilic Campylobacter spp. counts, while viable Campylobacter spp. counts remained almost at the inoculation level according to the v-qPCR analysis.
Article
Food Science & Technology
Sevinc Ferrari, Asgeir Astvaldsson, Therese Jernberg, Kerstin Stingl, Ute Messelhaeuber, Hanna Skarin
Summary: This article outlines the validation process and organization of three multiplex PCR methods for identifying and confirming thermotolerant Campylobacter spp. These methods were compared to the reference method described in the EN ISO standard 10272:2017 through a method comparison study and an interlaboratory study based on EN ISO 16140-6:2019. The performance of each PCR target, in terms of inclusivity and exclusivity, was found to be comparable or better than the reference method. Overall, all three PCR methods were deemed equally qualified as the reference method for molecular identification and confirmation of thermotolerant Campylobacter spp., C. jejuni, C. coli, and C. lari isolated from the food chain, and have been included in Amendment 1 of ISO 10272:2017.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Virology
Christian Willy, Joachim J. Bugert, Annika Y. Classen, Li Deng, Anja Duechting, Justus Gross, Jens A. Hammerl, Imke H. E. Korf, Christian Kuehn, Simone Lieberknecht-Jouy, Christine Rohde, Markus Rupp, Maria J. G. T. Vehreschild, Kilian Vogele, Sarah Wienecke, Martin Witzenrath, Silvia Wuerstle, Holger Ziehr, Karin Moelling, Felix Broecker
Summary: Bacteriophage therapy shows promise in addressing global and German antibiotic resistance crises. The current situation in Germany regarding phage therapy and research is outlined. Critical shortcomings include a lack of clinical trials, regulation, and phages for clinical use. Phage therapy is being used on a limited number of patients as individual treatment trials.
Article
Microbiology
Dorothee Serian, Yury Churin, Jens Andre Hammerl, Manfred Rohde, Arne Jung, Anja Mueller, Min Yue, Corinna Kehrenberg
Summary: In this study, seven Bordetella avium phages were isolated and characterized, showing broad host range and strong bacteriolytic activity. These phages used lipopolysaccharides as host receptors and exhibited a myovirus structure. Although phenotypically thought to be lytic, these phages were found to undergo a lysogenic phase, but infection did not confer stable host superinfection immunity. These findings are important for potential phage therapy against avian bordetellosis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Anna-Delia Knipper, Steven Goehlich, Kerstin Stingl, Narges Ghoreishi, Carola Fischer-Tenhagen, Niels Bandick, Bernd-Alois Tenhagen, Tasja Crease
Summary: This study investigated the variation in the prevalence and concentration of Campylobacter spp. in cows' milk, feces, and the farm environment over one year at a small German dairy farm. The highest prevalence of Campylobacter spp. was found in feces (77.1%), while it was completely absent in milking equipment and low in raw milk (0.4%). The study highlights the persistence of Campylobacter spp. in individual cows' intestines and the farm environment for at least one year, as well as the possibility of fecal cross-contamination of teats even when raw milk contamination is rare.
Article
Virology
Julia Anabell Braeuer, Jens Andre Hammerl, Sabrin El-Mustapha, Julius Fuhrmann, Andrea Barac, Stefan Hertwig
Summary: Telomere phages are a small group of temperate phages that replicate as linear plasmids and have a specific host specificity. Their unique characteristics make them important in research.
Article
Multidisciplinary Sciences
Mateusz Noszka, Agnieszka Strzalka, Jakub Muraszko, Rafal Kolenda, Chen Meng, Christina Ludwig, Kerstin Stingl, Anna Zawilak-Pawlik
Summary: This study presents a comprehensive investigation of the redox switch protein HP1021 regulon in the gastric human pathogen Helicobacter pylori. The findings suggest that HP1021 plays a crucial role in modulating the response to oxidative stress in H. pylori, controlling the transcription of numerous genes and influencing the expression of proteins involved in various pathways. Additionally, HP1021 is found to regulate competence and glucose consumption, impacting the energetic balance in the cell.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Ulrike Binsker, Kathrin Oelgeschlaeger, Bernd Neumann, Guido Werner, Annemarie Kaesbohrer, Jens A. Hammerl
Summary: The mcr-1.26 gene, carried by E. coli isolated from poultry samples in Germany, was found to be mainly transmitted through plasmids. The genetic sequences of the plasmids in poultry and human isolates were highly similar, suggesting that poultry husbandry is the primary source of mcr-1.26.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Benjamin Reichelt, Vanessa Szott, Kerstin Stingl, Uwe Roesler, Anika Friese
Summary: Campylobacteriosis cases, caused by Campylobacter bacteria, are a global concern, particularly in poultry reservoirs. The bacteria can enter a viable but non-culturable (VBNC) state, leading to an underestimation of Campylobacter prevalence. Research found that VBNC Campylobacter can persist in soil for up to 28 days and in water for at least 63 days.
Article
Microbiology
Yulan Su, Thomas Alter, Greta Goelz
Summary: The aim of this study was to investigate the impact of surrounding viscosity on the expression of motility related genes in Campylobacter jejuni. RNA was extracted from liquid cultures and bacterial cells recovered from the edge and center of a swarming halo. The study found different gene expression patterns and higher energy demand in high viscous media compared to liquid culture, indicating the importance of considering viscosity in motility related studies.
EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Microbiology
Quantao Zhang, Thomas Alter, Eckhard Strauch, Jens Andre Hammerl, Keike Schwartz, Maria Borowiak, Carlus Deneke, Susanne Fleischmann
Summary: This study characterized the virulence potential of NOVC strains isolated from retail seafood at both genotypic and phenotypic levels. The results showed differences in the virulence potential among the analyzed strains, which may have implications for future seafood monitoring and risk assessment.